Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	2,251,239	G→A	P28P (CCC→CCT)	LY180_10740 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	2,251,239	0	G	A	100.0%	13.5 / NA	10	P28P (CCC→CCT)	LY180_10740	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/4);  total (6/4)

TTCGGTCAGGCTCATGGGATACAGTTCTGATGGCGGCCAGTGAAATATCACTGCCACATCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTGGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCAATCATGTTGATGGTGTCGA  >  CP006584/2250949‑2251455                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                         ttCGGTCAGGCTCATGGGATACAGTTCTGATGGCGGCCAGTGAAATATCACTGCCACATCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCCTTAGGTTtca                                                                                                                                                                                                                   >  1:185972/1‑298 (MQ=21)                                                    ggcaacttCCGCCATCAGGTATTTGACCGGGAGATTTTCCGGGAACATCACTGCACCGGGTTCGGCAACAAAAAACCGACCGCCCTACCGGCCCGCGCCACAAGGTCAGGCAGTTCCAGGGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCa                                                                                                                                                                <  2:311966/291‑1 (MQ=11)                                                                                                              aGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGt                                                                                                      <  1:142916/298‑1 (MQ=18)                                                                                                               gTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTc                                                                                                     >  1:255399/1‑298 (MQ=18)                                                                                                                                             ggCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATCACCTGCTCACCACGCGTGACCGGATTGCCCAGGGTCATCACATTTTCTTTGTTCATTGTTTCCTCACTTCTGAATCGTGGTACCCCGTTCAGCCCGGCTGTCCGCACGACCATcaccagc                                                                       >  2:205537/1‑294 (MQ=11)                                                                                                                                                                 gCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAAACACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGc                                                   >  1:154327/1‑298 (MQ=18)                                                                                                                                                                    gTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGTGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTcc                                                <  1:92493/298‑1 (MQ=18)                                                                                                                                                                                                             gTCATGCGCGGCAGCACCTTAATCAGTGCATCGACCTCGGAGTTTGCGACCGCAGCCCGCCTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGCTCGCTAACCTGCCCACCACGCTTGACCGGATTGCCCAGGGTAATCACATTTTCTTTGGTCATGGTTTTCCCACTTCTGCATCGGGGTTAACCGGTCAGCCAGGCTGACCGGCTGAAAATCACACGCCGATATTGCCGCGGCGCTGCTCCAGCCGGTCAACGCCGTTCACCTCCTCACTCCTGttcagagc       >  2:36883/1‑292 (MQ=255)                                                                                                                                                                                                                  gcgcGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTAGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCAATCATGTTGATGGTGTCGa  <  1:303724/298‑1 (MQ=14)                                                                                                                                                                                                                  gcgcGGCAGCACCTTAATCAGTGCATCGACGTCGGCGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCACCCAATTAGGTTTCATCAGCGTGCACTGTTAGATAACCTGCTCACACCGCTTGCCCCGATTGTCCAGCGTCATCACATTTTCTTTGTTCATGGTTTTCTACCTTCTGCATCCGGGTTAACCGGACAGCCAGGCTGACCTGCTGCAACTCCCAGGCCGACATATCGTAGGTCTTCCTCCAGCCGGCCTACCCCTTTCGCCTTCTCCCTCATGTTGaagatgcgga  >  2:246282/1‑289 (MQ=2)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                         TTCGGTCAGGCTCATGGGATACAGTTCTGATGGCGGCCAGTGAAATATCACTGCCACATCCGCCATCAGGTCATCGACCGAGAGATTTTTCGGGAACGTCACTGCACCGAGTTCGGCGACAAAAAACCGACCACCTTACCGGCCAGCGCCACAAGGTCAGGCAGTTCCAGTGCGGCGACCTCCTGCTCGGTCAGCATCGGTGCCGTCATGCGCGGCAGCACCTTAATCAGTGCATCGACTTCGGAGTTTGCGACCGCAGCCAGACTGACACCGCGCAGCGTCCCGGCATTGGGTTTCATCAGCGTGACCTGTTCGATAACCTGCTCACCACGCTTGACCGGATTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCAATCATGTTGATGGTGTCGA  >  CP006584/2250949‑2251455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑