Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	2,483,647	G→A	P236S (CCG→TCG)	LY180_11800 ←	NADH:ubiquinone oxidoreductase subunit M

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	2,483,647	0	G	A	100.0%	63.4 / NA	21	P236S (CCG→TCG)	LY180_11800	NADH:ubiquinone oxidoreductase subunit M
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/9);  total (12/9)

TCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGGCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGA  >  CP006584/2483371‑2483934                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                tCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGAt                                                                                                                                                                                                                                                                            <  1:190020/298‑1 (MQ=255)                    ggAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAAc                                                                                                                                                                                                                                                         <  1:84914/298‑1 (MQ=255)                             ggTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTcc                                                                                                                                                                                                                                                >  1:37247/1‑298 (MQ=255)                                gtAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCcaca                                                                                                                                                                                                                                             <  1:170774/298‑1 (MQ=255)                                                                   aaGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGCAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATa                                                                                                                                                                                                          <  1:17253/298‑1 (MQ=255)                                                                                                  aaCCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAg                                                                                                                                                                           >  1:2319/1‑298 (MQ=255)                                                                                                    cccccAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCCCTGGACATTGGCGCTATCATCACGCCTTCCTAGTCGAAGGTCCAGACGCCGGGCCCAATGAAGTg                                                                                                                                                                         >  2:155433/1‑298 (MQ=255)                                                                                                         aGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCTGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGCGGTCAACGGAACCGGCGGGCGGAGCCTGAGAGTGCGCATCCGGTCGCCAGCCATGCCGCAGCACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTACCAGGTATTCCACACCACTGCACATGCGCATATTCAGCAGCTCTCCATATATCACGGCCGAGACGCCACTGGCCTTGTCGTGAACaa                                                                                                                                                                    >  2:99151/1‑298 (MQ=255)                                                                                                            cacaTAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAGCCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTACGAAGGCGATGAAGAAGACCAGCATTAACAGGTATTAGACACCACTGGACATTGTCGTATTGGGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGGATTGTAGTGAACaaaaa                                                                                                                                                                 >  1:191639/1‑298 (MQ=255)                                                                                                             acagggcgtgtcgcgcAGACTGTCACGATGCGCGCGGGAACAGAGGCAGGGAGAACGGGAGCAAACCGTAAGAGGCAGTGTTCAGAAAGGTCGCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCGGAGAGTGCGCATCCGGTAGCCAGCGATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAAc                                                                                                                                                                <  2:37247/286‑1 (MQ=255)                                                                                                              caTAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAAcc                                                                                                                                                               >  1:88239/1‑298 (MQ=255)                                                                                                                                       gATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGGATTCAGCAGCTCTTCATAGTTGAAGGGCCAAGCGCCGGCCCCATTGTAGTGAACGAAAACCAGCCCCAGGATGGCCaacaaccaca                                                                                                                                      >  2:298936/1‑294 (MQ=255)                                                                                                                                                                         gcaAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACtt                                                                                                    <  1:155433/298‑1 (MQ=255)                                                                                                                                                                                         tctccaatctaaGAGCCCCGCAAGGTCAACGGAACCGGCGGTCGGGGCCGGAGGGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATa                                                                                    <  2:221566/288‑1 (MQ=255)                                                                                                                                                                                          gTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATAc                                                                                   <  1:246729/298‑1 (MQ=255)                                                                                                                                                                                                    aGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATAcagatcggaag                                                                         >  1:221566/1‑288 (MQ=255)                                                                                                                                                                                                              gAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCt                                                               >  1:296485/1‑298 (MQ=255)                                                                                                                                                                                                                                     gAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAg                                        <  1:275957/298‑1 (MQ=255)                                                                                                                                                                                                                                            agagTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTAc                                 >  1:274608/1‑298 (MQ=255)                                                                                                                                                                                                                                                        cGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCCGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATTCCCCCACAGTGCGAGCAGGAAGTACAGCGGCACCCGc                     >  2:244045/1‑298 (MQ=255)                                                                                                                                                                                                                                                                           ggAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCagaagaaga  >  1:179844/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                                TCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGGCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGA  >  CP006584/2483371‑2483934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑