Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	3,010,362	G→T	E48* (GAA→TAA)	LY180_14330 →	type VI secretion protein ImpK

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	3,010,362	0	G	T	100.0%	70.1 / NA	22	E48* (GAA→TAA)	LY180_14330	type VI secretion protein ImpK
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/14);  total (8/14)

CCGCTGCGACTGGAGAACCAGTATTTTGCGCTGGATATGCACAGTGACGCGGCTAAATCGATGCTGGAGTCCGGGTGCTGCATGATTTATGCGCCAGGAACCATGGGCGACCTGAAACCTGAACTGTTTGCGGTGCTGCGCGCATGAAAAAAGAGACGGATATCAATATCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCGAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCTGGATGAAGGATCCACTCCAGTCGCATTTCTTCAACACCCTGCAGGCAGGCGAGCTGCTGTACGAGCGGATGAAGCAGGTGTTGCAGGAGCCCGCGCCTGCACAGGCTGTGCTGACCTGCTTTCACCGTGTCCTGCTGCTGGGATTTCGCGGGCGTTATCAGGATCC  >  CP006584/3010078‑3010648                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                               ccgctgcGACTGGAGAACCAGTATTGGGCGCTGGATATGCACAGTGACGCGGCTAAATCGATGCTGGAGTCCGGGTGCTGCATGATTTATGCGCCAGGAACCATGGGCGACCTGAAACCTGAACTGTTTGCGGTGCTGCGCGCATGAAAAAAGAGACGGATATCAATATCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGa                                                                                                                                                                                                                                                                                   <  1:210914/298‑1 (MQ=255)               ggaCCAGGTTTTTGGGCTGGTTATGACCAGGGAGGCGACGAAATCGGTGCTGGAGTCCGGGTGCTGCATGATTTATGCGCCAGGAACCATGGGCGACCTGAAACCTGAACTGTTTGCGGTGCTGCGCGCATGAAAAAAGAGACGGATATCAATATCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGcc                                                                                                                                                                                                                                                                     <  2:14093/296‑1 (MQ=255)                aaCCAGTATTTTGCGCTGGATATGCACAGTGACGCGGCTAAATCGATGCTGGAGTCCGGGTGCTGCATGATTTATGCGCCAGGAACCATGGGCGACCTGAAACCTGAACTGTTTGCGGTGCTGCGCGCATGAAAAAAGAGACGGATATCAATATCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCg                                                                                                                                                                                                                                                                    >  1:14093/1‑298 (MQ=255)                                             tGACGCGGCTAAATCGATGCTGGAGTCCGGGTGCTGCATGATTTATGCGCCAGGAACCATGGGCGACCTGAAACCTGAACTGTTTGCGGTGCTGCGCGCATGAAAAAAGAGACGGATATCAATATCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGTAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACgcgc                                                                                                                                                                                                                                       <  1:214630/298‑1 (MQ=255)                                                                                ggatgtgttAGGCGCCAGGAACGATGGGCGACCGGAACGCTGAACTGTTTGCGGGGCTGCGCGCAGGACAAAAGAGACGGGTGCCAATAGCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGGCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACAGTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGc                                                                                                                                                                                                    <  2:72566/291‑1 (MQ=255)                                                                                   tGATTTATGCGCCAGGAACCATGGGCGACCTGAAACCTGAACTGTTTGCGGTGCTGCGCGCATGAAAACAGAGACGGATATCAATATCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGCCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGACCTGGTCCGCTAACGCCTGAAAAATGCCGGGTTTCGCCGGGAGAGCGTGGAGCACATTACATCCGCCCCGTGGCCCCCGCGGGGCGACACAGAACTGGGTCgaggg                                                                                                                                                                                                 >  2:22341/1‑294 (MQ=255)                                                                                                    aCCATGGGCGACCTGAAACCTGAACTGTTTGCGGTGCTGCGCGCATGAAAAAAGAGACGGATATCAATATCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCa                                                                                                                                                                                <  1:236147/298‑1 (MQ=255)                                                                                                            cGACCTGAAACCTGAACTGTTTGCGGTGCTGCGCGCATGAAAAAAGAGACGGATATCAATATCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCt                                                                                                                                                                        <  1:309087/298‑1 (MQ=255)                                                                                                                 tgaaacctgaaCTGTTTGCGGTGCTGCGCGCATGAAAAAAGAGACGGATATCAATATCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCTGGATg                                                                                                                                                                   >  1:138504/1‑298 (MQ=255)                                                                                                                         gaaCTGTTTGCGGTGCTGCGCGCATGAAAAAAGAGACGGATATCAATATCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCTGGATGAAGGATcc                                                                                                                                                           >  1:33702/1‑298 (MQ=255)                                                                                                                         gaaCTGTTTGCGGTGCTGCGCGCATGAAAAAAGAGACGGATATCAATATCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCTGGATGAAGGATcc                                                                                                                                                           <  1:91863/298‑1 (MQ=255)                                                                                                                                            gcgcATGAAAAAAGAGACGGATATCAATATCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCTGGATGAAGGATCCACTCCAGTCGCATttcttc                                                                                                                                        <  1:151986/298‑1 (MQ=255)                                                                                                                                                             cGGATATCAATATCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCTGGATGAAGGATCCACTCCAGTCGCATTTCTTCAACACCCTGcaggcagg                                                                                                                       <  1:82701/298‑1 (MQ=255)                                                                                                                                                                atatCAATATCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCTGGATGAAGGATCCACTCCAGTCGCATTTCTTCAACACCCTGCAGGCAGGCGa                                                                                                                    >  1:141260/1‑298 (MQ=255)                                                                                                                                                                       tatCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTAAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCTGGATGAAGGATCCACTCCAGTCGCATTTCTTCAACACCCTGCAGGCAGGCGGGctgctg                                                                                                             >  1:70098/1‑298 (MQ=255)                                                                                                                                                                                        gcgACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCTg                                                                                                                                                                       >  1:182972/1‑223 (MQ=255)                                                                                                                                                                                        gcgACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCTg                                                                                                                                                                       <  2:182972/223‑1 (MQ=255)                                                                                                                                                                                          gACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCTGGATGAAGGATCCACTCCAGTAGCATTTCTTCAACACCCTGCAGGCAGGCGAGCTGCTGTACGAGCGGATGAAGCAgg                                                                                          >  1:103905/1‑298 (MQ=255)                                                                                                                                                                                                 ttCTGACGGTTGTGGAACTGCGTAAGGGAACCACCGTCCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCTGGATGAAGGATCCACTCCAGTCGCATTTCTTCAACACCCTGCAGGCAGGCGAGCTGCTGTACGAGCGGATGAAGCAGGTGTTGCa                                                                                   <  1:136800/298‑1 (MQ=255)                                                                                                                                                                                                                                                             gTCACTGCCAGCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTACTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCTGGATGAAGGATCCACTCCAGTCGCATTTCTTCAACACCCTGCAGGCAGGCGAGCTGCTGTACGAGCGGATGAAGCAGGTGTTGCAGGAGCCCGCGCCTGCACAGGCTGTGCTGACCTGCTTTCACCGTGTCCTGCTGCTGGGAtt                       <  1:22341/298‑1 (MQ=255)                                                                                                                                                                                                                                                                       gCGGCAGGTGGAACTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCTGGATGAAGGATCCACTCCAGTCGCATTTCTTCAACACCCTGCAGGCAGGCGAGCTGCTGTACGAGCGGATGAAGCAGGTGTTGCAGGAGCCCGCGCCTGCACAGGCTGTGCTGACCTGCTTTCACCGTGTCCTGCTGCTGGGATTTCGCGGGCGt             <  1:111525/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aaCTGGTCCGCTAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCTGGATGAAGGATCCACTCCAGTCGCATTTCTTCAACACCCTGCAGGCAGGCGAGCTGCTGTACGAGCGGATGAAGCAGGTGTTGCAGGAGCCCGCGCCTGCACAGGCTGTGCTGACCTGCTTTCACCGTGTCCTGCTGCTGGGATTTCGCGGGCGTTATCAGGATcc  <  1:57196/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                                                               CCGCTGCGACTGGAGAACCAGTATTTTGCGCTGGATATGCACAGTGACGCGGCTAAATCGATGCTGGAGTCCGGGTGCTGCATGATTTATGCGCCAGGAACCATGGGCGACCTGAAACCTGAACTGTTTGCGGTGCTGCGCGCATGAAAAAAGAGACGGATATCAATATCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAACCACCGTGCGTCACGGTATGGAGCTGTATCGTCACTGCCAGCGGCAGGTGGAACTGGTCCGCGAACGCCTGAAAGATGCCGGGTTTAGCCGGGAGAGCGTGGAGCACATTACCTACGCGCAGTGCGCCCTGCTGGACGAAACAGTCCTGAGTCGCAGCGGCATGGACGACGGTCAGGCCATCTGGATGAAGGATCCACTCCAGTCGCATTTCTTCAACACCCTGCAGGCAGGCGAGCTGCTGTACGAGCGGATGAAGCAGGTGTTGCAGGAGCCCGCGCCTGCACAGGCTGTGCTGACCTGCTTTCACCGTGTCCTGCTGCTGGGATTTCGCGGGCGTTATCAGGATCC  >  CP006584/3010078‑3010648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑