Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	3,278,551	G→A	W246* (TGG→TGA)	LY180_15575 →	aldehyde oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	3,278,551	0	G	A	94.4%	104.0 / NA	36	W246* (TGG→TGA)	LY180_15575	aldehyde oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (18/16);  minor base C (2/0);  total (20/16)
Fisher's exact test for biased strand distribution p-value = 4.92e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

CGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGGGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTC  >  CP006584/3278262‑3278844                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                      cgGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGGCATGTGAGGGGCGAc                                                                                                                                                                                                                                                                                               >  1:193222/1‑298 (MQ=255)      aTCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAATAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGGGAGGGGCGACGCAgg                                                                                                                                                                                                                                                                                          >  1:44506/1‑298 (MQ=255)                             cAAGCGGGCGTCCCGGTCGGCCCCTGTTTAGCCGGTAGTGGCCGTGGTCGTTCCGGTTTAGACCTGCGCCCGGCCGCGGCGTTAGGTGGCTAACGGCGTAGGGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGACAAAATTCAGGACCGTTTCGCAGAAGGCATGTTGCTGACGCTGAGCGAAGATGGTCCGAAAGCCCCGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCt                                                                                                                                                                                                                                                                   <  2:287939/298‑1 (MQ=255)                                 cAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTCCACACCGTGGAACCGTATGTTACCAAAACGGGTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGAATTTTGCTGACGCTGAACGAAGATGGTCCGAAAGCCTTGCACGAGCCAGAAAACTACGACGCTCGCGCCAACGTACTGGACAGGGCGACCCAGGCGCGGAAAGGGTTGATTGGCACTGGct                                                                                                                                                                                                                                                               >  2:137817/1‑297 (MQ=255)                                   ggCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCCGACGGCATTTTGCTGACGCTGATCGAAGATGGTCCGCAAGCCCTGAAAGAGCCAGAAAACTACGCAGTGCGCCCCAACGTCACGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTTGCCCTTGTGTa                                                                                                                                                                                                                                                             >  2:49298/1‑298 (MQ=255)                                              cTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTg                                                                                                                                                                                                                                                  <  1:237099/298‑1 (MQ=255)                                              cTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCACAAGGCATTTTTCTGACCCTGATCGAAGAGGGGCCGCAAGCCCTGACAGAGCCAGCAAACTACGACGGCCGGCCCAAAGTCAAGTGAGGGGCGACGCAGGCGCAGCACGGTGTGAGTGCAGCTGGCGtccagcgagacag                                                                                                                                                                                                                                                  >  2:20826/1‑286 (MQ=255)                                                      gTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCCCCAACGCCATGTGCGGGGCGACGCAGGCGCCGAACGGTTTGATTGCCGGCGGCCTACCGCAGGAAGGGGccaagc                                                                                                                                                                                                                                          >  2:58060/1‑293 (MQ=255)                                                          ggCCGGTGTTTGGCCTGATCGATCCGGGTTATGCCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACCGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGAGCGGTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCatat                                                                                                                                                                                                                                      <  2:123907/297‑1 (MQ=255)                                                                tATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTggg                                                                                                                                                                                                                                >  1:324123/1‑298 (MQ=255)                                                                         tGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAact                                                                                                                                                                                                                       <  1:137817/298‑1 (MQ=255)                                                                                                                 ggTGGCTAACGGCGTCGTGGACGCCTTTGTCCACACCGTGGAACAGTATGTTACCAAACAGGTGCATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCTAAGATGGTCCGAAAGCCCTGAAAGCGCCAGAAAACTACGATGTGCACGCCAACGTCCTGTAAGGGGCGACGCAGGCGCGGAACGGGATGATTGGCGCTGGCGTACCGCAGGACAGAGCAACGCACATCCTGGGCAACGCACTACACGCGATGCACGGTCAGGAGCACGCGCACGCACAGGCt                                                                                                                                                                               >  2:236857/1‑298 (MQ=255)                                                                                                                       tAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTc                                                                                                                                                                         <  1:145486/298‑1 (MQ=255)                                                                                                                                    gACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACtgt                                                                                                                                                            <  1:61784/298‑1 (MQ=255)                                                                                                                                               acacacCGTGGACCAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGCGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCCACGCAGGCGCTGCACGGTTTGATTTGCGCTGGCGTACCGCAGGACTGGGCAGCGCATCTGCTGGGACACGAACTGCCTGCAATGCCCGGTCTGGATCCCGACCACACACCTGCCAACCTCCTTGCCGCACAGTGGCATGaaaac                                                                                                                                                 >  2:269700/1‑297 (MQ=255)                                                                                                                                               acacacCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGaaaaa                                                                                                                                                 >  1:87858/1‑298 (MQ=255)                                                                                                                                                      gTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCgaga                                                                                                                                          <  1:269873/298‑1 (MQ=255)                                                                                                                                                                 tGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCCCTGTGGAATGAAAAACGCGAGACCAAGCGCGCt                                                                                                                               >  1:9460/1‑298 (MQ=255)                                                                                                                                                                      ccAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAgct                                                                                                                          <  1:160923/298‑1 (MQ=255)                                                                                                                                                                                 gATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGTGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATg                                                                                                               <  1:49298/298‑1 (MQ=255)                                                                                                                                                                                             cAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGACCGAAAGCCCCGAAAGAGCCAGAAACCTACGATGCGCGCGCCACCGTCATGTGAGGGGGGACGCAGGAGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGCTCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCt                                                                                                   <  1:12018/298‑1 (MQ=255)                                                                                                                                                                                                           aGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGaa                                                                                     <  1:146281/298‑1 (MQ=255)                                                                                                                                                                                                            gAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAg                                                                                    >  1:141614/1‑298 (MQ=255)                                                                                                                                                                                                                        cTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGatgatg                                                                        <  1:58060/298‑1 (MQ=255)                                                                                                                                                                                                                         tGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGAtgatga                                                                       >  1:72304/1‑298 (MQ=255)                                                                                                                                                                                                                                 aTCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTg                                                               >  1:147370/1‑298 (MQ=255)                                                                                                                                                                                                                                      aGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGAcgcc                                                          >  1:11168/1‑298 (MQ=255)                                                                                                                                                                                                                                                        gAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGc                                        >  1:4185/1‑298 (MQ=255)                                                                                                                                                                                                                                                        gAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCCGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTATGGATCACGCGCAAACACTGGCTATCCGCCTGCCTGCACTGTGGACCGAAAAACGCGAGACCAAGCGCGCTAACCTGCCTCAATATGCTAAACCCGTCTGGAACATCGCTGAAGGTTCAGATCATGCGCGTATTAACGCCCCACTTGCCTCacaccga                                        >  2:164504/1‑292 (MQ=255)                                                                                                                                                                                                                                                                 aGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCttt                               <  1:269700/298‑1 (MQ=255)                                                                                                                                                                                                                                                                  gAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTg                              <  1:135426/298‑1 (MQ=255)                                                                                                                                                                                                                                                                         aCGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGCCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCCACGCCGCTCCAATATGCGTAACGCGTCTGGAACACCACCGAAGGTTCCGATGATAGGCCTACTGCCCCCGCGCTTGCCCCCACCCCCCATTTCcttcgccaaag                       >  2:156089/1‑290 (MQ=255)                                                                                                                                                                                                                                                                          cGAGGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTa                      <  1:20826/298‑1 (MQ=255)                                                                                                                                                                                                                                                                               tgCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGTTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGt                 >  1:334452/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                  gcgcCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGcc              <  1:332355/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                             aTGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACctct   >  1:120854/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                              tggggggggggTCGCAGGGGCTGAATGGTTTGGTGGGCTCGAGAGTACGGCAGGAGTGGTGAACGCATATGCGTGGCCACGAACGGATTGGTATGCACGGTCTTGATCAGGCGAGAACACTGGTTATCGTCCTGCCTGCACGGGGGAATGAAAACCGGGGGACTAAGTGGGCTAAGCTGCTGCAATCTGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGCTGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCGTTGAGCAATTAGGCGTGCCGACCCACGtctc  <  2:147370/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                      CGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGGGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCTC  >  CP006584/3278262‑3278844

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑