Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	4,305,201	C→G	A68G (GCC→GGC)	LY180_20645 →	rhsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	4,305,201	0	C	G	94.1%	41.0 / ‑6.6	17	A68G (GCC→GGC)	LY180_20645	rhsA
Reads supporting (aligned to +/- strand):  ref base C (0/1);  new base G (8/8);  total (8/9)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGCCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACTCAGTGATAACGGCGGCAGAAGCCTGTATTTTGAGCACCTGTTTCCCGGTGAGGACGGTTACAGCCGCAGCGAGTCACTGTGGCTGGTGCGCGGCGGCGTGGCGAAACTGGATGAAGGTCACCGGCTGGCCGCACTCTGGCAGGCGCTGCCG  >  CP006584/4304908‑4305487                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                               tGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTCTGGCGGTAGAATTGTCCACGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCGGTTCCGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGGCAATCCCCTGCCCGGTACAACGGTCCTTCCCGGCTACACCGCCAACCCCCCCcccggccca                                                                                                                                                                                                                                                                                            >  2:326831/1‑297 (MQ=18)  gAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGcccggcccgc                                                                                                                                                                                                                                                                                           <  1:234165/298‑1 (MQ=255)      aaTTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGct                                                                                                                                                                                                                                                                                       >  1:252018/1‑297 (MQ=255)         tCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGtt                                                                                                                                                                                                                                                                                    <  1:317044/298‑1 (MQ=255)            ggggTGTTAACTCATTATCATTTCCTGTTGTTGACTGGTGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCCGCCGGGGTACGTCTTGGTGCCCCCACCGGCGTGGCCTGTTCGGTTTGCCCCGGCGGAGCGACGACCGGCCCTCCGGTCACTCCCTTGCCCGGCGCCAAGGGCCTTCCCGTCAAACCCGACCTCGCCATCCCCGGCCcgccgcactgccc                                                                                                                                                                                                                                                                                 >  2:160653/1‑291 (MQ=18)             gggTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATc                                                                                                                                                                                                                                                                                >  1:215659/1‑298 (MQ=255)                  ttAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCtctc                                                                                                                                                                                                                                                                           <  1:326831/298‑1 (MQ=255)                                 ttCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTAcagcag                                                                                                                                                                                                                                                            <  1:262299/298‑1 (MQ=255)                                                   gcTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGcc                                                                                                                                                                                                                                          <  1:100374/298‑1 (MQ=255)                                                      aGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCcgc                                                                                                                                                                                                                                       >  1:145537/1‑298 (MQ=255)                                                          ttCTGGTTCTTATTTATTTCAAAAGGTTTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCTGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCATCTACAGCAGTTACCGGACAAAAACGCCCGCGCCg                                                                                                                                                                                                                                   <  1:104399/298‑1 (MQ=255)                                                                   ttatttatTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGc                                                                                                                                                                                                                          >  1:74888/1‑298 (MQ=255)                                                                      tttatttCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGGCTc                                                                                                                                                                                                                       >  1:125313/1‑298 (MQ=255)                                                                                           gATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCt                                                                                                                                                                                                  >  1:163673/1‑298 (MQ=14)                                                                                                 cGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGat                                                                                                                                                                                            <  1:84293/298‑1 (MQ=14)                                                                                                             ggcgCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCTCGGCTGGAAAATGCCTGCGGATATCCGCTTACAg                                                                                                                                                                                <  1:230722/298‑1 (MQ=11)                                                                                                             ggcgCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTACCCCGGCGGAGTGACGTCCGGCCATCCGATCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCAGGTGGGGAGCCTCGGCCCCGGCTGGGAGATGCCCGCGGATAAACGCTTACAg                                                                                                                                                                                >  1:102937/1‑298 (MQ=11)                                                                                                                 cgtgcgggTGACATGACGGAGTATTGGGGTGGGATTGTCCGGGGGTGAGCGGGGGGACGTGTTGGTGCCCCCCCCGGCGTGGCCTGTTGGGTGTGCCCGGGCGGGGGGACGTCCGGCCATCCGGGCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCTGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTgc                                                                                                                                                                            <  2:252018/293‑1 (MQ=11)                                                                                                                                                                                                                                                                                           cGCCCTGCCCGCCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACTCAGTGATAACGGCGGCAGAAGCCTGTATTTTGAGCACCTGTTTCCCGGTGAGGACGGTTACAGCCGCAGCGAGTCACTGTGGCTGGTGCGCGGCGGCGTGGCGAAACTGGATGAAGGTCACCGGCTGGCCGCACTCTGGCAGGCGCTGCCg  <  1:78135/298‑1 (MQ=31)                                                                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                               TGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGCCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACAGCTGCGCGATAACACACTGATACTCAGTGATAACGGCGGCAGAAGCCTGTATTTTGAGCACCTGTTTCCCGGTGAGGACGGTTACAGCCGCAGCGAGTCACTGTGGCTGGTGCGCGGCGGCGTGGCGAAACTGGATGAAGGTCACCGGCTGGCCGCACTCTGGCAGGCGCTGCCG  >  CP006584/4304908‑4305487

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑