breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 21 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0065843,849,2980CT70.5% 78.6 / 37.8 63R121C (CGC→TGC) LY180_18350transcriptional regulator
*CP0065841,643,5820GA64.6% 58.1 / 58.7 66intergenic (‑242/‑61)LY180_07760/LY180_07765sulfatase/transposase
*CP006584269,4380TC45.6% 8.4 / 50.2 47L55P (CTC→CCC) LY180_01185hypothetical protein
*CP006584269,4520CT44.7% 13.8 / 49.7 48Q60* (CAA→TAA) LY180_01185hypothetical protein
*CP006584269,4920CT43.8% 20.8 / 47.3 49T73I (ACC→ATC) LY180_01185hypothetical protein
*CP0065843,513,9650TC43.8% ‑0.6 / 16.9 16intergenic (‑141/+198)tldD/LY180_16725protease TldD/membrane protein
*CP006584269,4430TG42.5% 4.6 / 61.6 47F57V (TTT→GTT) LY180_01185hypothetical protein
*CP006584269,4530AG42.5% 9.9 / 53.0 47Q60R (CAA→CGA) LY180_01185hypothetical protein
*CP0065844,254,0250GA42.0% 23.1 / 50.0 50A28A (GCG→GCALY180_20340hypothetical protein
*CP006584269,4000GA41.3% 25.4 / 40.5 46E42E (GAG→GAALY180_01185hypothetical protein
*CP006584269,1160CT40.9% 15.4 / 40.0 45R121R (CGC→CGTLY180_01180hypothetical protein
*CP006584269,4030TC39.1% 20.0 / 43.3 46G43G (GGT→GGCLY180_01185hypothetical protein
*CP0065844,253,9760TA38.3% 49.2 / 69.2 61F12Y (TTC→TAC) LY180_20340hypothetical protein
*CP0065844,253,9020CT31.8% 84.6 / 49.8 68intergenic (+24/‑40)LY180_20335/LY180_20340hypothetical protein/hypothetical protein
*CP0065841,643,6180CG28.6% 106.4 / 50.5 64intergenic (‑278/‑25)LY180_07760/LY180_07765sulfatase/transposase
*CP0065844,253,8790GA28.6% 105.4 / 34.3 63intergenic (+1/‑63)LY180_20335/LY180_20340hypothetical protein/hypothetical protein
*CP006584269,0440TC27.7% 56.5 / 29.0 48C97C (TGT→TGCLY180_01180hypothetical protein
*CP006584269,0410TC25.5% 63.8 / 24.8 48D96D (GAT→GACLY180_01180hypothetical protein
*CP0065841,643,6420AG25.0% 109.1 / 41.5 72intergenic (‑302/‑1)LY180_07760/LY180_07765sulfatase/transposase
*CP0065844,254,1360CT23.3% 67.8 / 18.9 44N65N (AAC→AATLY180_20340hypothetical protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP006584 4739807 =41 (1.460)5 (0.180) 5/568 2.7 11.8% intergenic (+48/‑432) LY180_22625/fimA tyrosine recombinase/type‑1 fimbrial protein subunit A
?CP006584 4740121 = 35 (1.290)intergenic (+362/‑118) LY180_22625/fimA tyrosine recombinase/type‑1 fimbrial protein subunit A
* ? CP006584 = 381523926 (0.930)6 (0.220) 3/562 3.4 19.4% intergenic (‑652/+261) LY180_18180/LY180_18185 peptide ABC transporter substrate‑binding protein/tRNA‑Pro
?CP006584 = 4312417 NA (NA)intergenic (‑191/+57) LY180_20660/LY180_20665 hypothetical protein/transcriptional regulator
* ? CP006584 = 367595134 (1.210)3 (0.110) 3/586 3.6 9.0% coding (2284/2448 nt) LY180_17595 glycogen phosphorylase
?CP006584 4110793 = 27 (0.960)coding (569/1047 nt) LY180_19600 lipopolysaccharide biosynthesis protein WzzE
* ? CP006584 = 25812733 (1.180)3 (0.110) 3/586 3.6 7.1% coding (1379/1476 nt) LY180_01140 type VI secretion protein
?CP006584 = 4456821 45 (1.600)intergenic (+160/‑93) alr/LY180_21295 alanine racemase/aromatic amino acid aminotransferase