New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	CP006584	346068 =	NA (NA)	37 (0.890)	35/586	0.3	100%	intergenic (‑51/‑107)	LY180_01675/LY180_01680	CadC family transcriptional regulator/hypothetical protein
	?	CP006584	= 363812	0 (0.000)					intergenic (‑39/‑249)	LY180_01770/LY180_01775	outer membrane phosphoporin protein E/gamma‑glutamyl kinase

ATCGATAGCAACGCGCAACGCATGGATCGCCGTCGGCAGGCTGTTTCCGCCGACAATGCGGTTCTTCCATACTTCGGCACATAAATAAGATCGCGATAAAACCACATCGGCATTTTTTGCTAATGTTTCCAGCAAAATAAAATGGTATTCGCCGAGACGACGTGTTTCACCCGTTTCGCAATGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAACGACTCAACAGCTTAACGTTGGCTTGCCACGCATTACTTGACTGTAAAACTCTCACTCTTACCGAACTTGGCCGTAACCTGCCAACCAAAGCGAGAACAAAACATAACATCAAACGAATCG  >  CP006584/345781‑346358                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                   atcGATAGCAACGCGCAACGCATGGATCGCCGTCGGCAGGCTGTTTCCGCCGACAATGCGGTTCTTCCATACTTCGGCACATAAATAAGATAGCGATAAAACCACATCGGCATTTTTTGCTAATGTTTCCAGCAAAATAAAATGGTATTCGCCGAGAAGACGTGTTTCACCCGTTTCGCAATGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGCTAAATGTTACTTGATAGTGCTTTGCACTGATGAATcc                                                                                                                                                                                                                                                                                          >  1:325034/1‑298 (MQ=255)    gATAGCAACGCGCAACGCATGGATCGCCGTCGGCAGGCTGTTTCCGCCGACAATGCGGTTCTTCCATACTTCGGCACATAAATAAGATCGCGATAAAACCACATCGGCATTTTTTGCTAATGTTTCCAGCAAAATAAAATGGTATTCGCCGAGACGACGTGTTTCACCCGTTTCGCAATGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACACATAGTTGGGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGGGTTTTGCATTGATGAATCCCCt                                                                                                                                                                                                                                                                                       >  2:264414/1‑298 (MQ=255)     attccaagaggacacgtATGGTTCGCCGTCGGCGGGCTTTTTCCGCGGCAAATGAGGTCTTTCCGTATTTGGGCACATAAAGAAGAGCGCGATAAAACCACATCTGCCTTTTTTGCTAATGTTTCCAGCAAAATAAAATGGTATTCGTAGAGACGACGGGTTTCACCCGTTTCGCAATGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTa                                                                                                                                                                                                                                                                                      <  2:300467/285‑1 (MQ=255)           acgcgcaacgcATGGATCGCCGTCGACAGGCTGTTTCCGCCGACAATGCGGTTCTTCCATACTTCGGCACATAAATAAGATCGCGATAAAACCACATCGGCATTTTTTGCTAATGTTTCCAGCAAAATAAAATGGTATTCGCCGAGACGACGTGTTTCACCCGTTTCGCAATGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGAtt                                                                                                                                                                                                                                                                                >  1:217404/1‑298 (MQ=255)                         gATCGCCGTCGGCAGGCTGTTTCCGCCGACAATGCGGTTCTTCCATACTTCGGCACATAAATAAGATCGCGATAAAACCACATCGGCATTTTTTGCTAATGTTTCCAGCAAAATAAAATGGTATTCGCCGAGACGACGTGTTTCACCCGTTTCGCAATGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCAt                                                                                                                                                                                                                                                                  <  1:2254/298‑1 (MQ=255)                                   ggCAGGCTGTTTCCGCCGACAATGCGGTTCTTCCATACTTCGGCACATAAATAAGATCGCGATAAAACCACATCGGCATTTTTTGCTAATGTTTCCAGCAAAATAAAATGGTATTCGCCGAGACGACGTGTTTCACCCGTTTCGCAATGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAgg                                                                                                                                                                                                                                                        >  1:368669/1‑298 (MQ=255)                                                    gACAATGCGGTTCTTCCATACTTCGGCACATAAATAAGATCGCGATAAAACCACATCGGCATTTTTTGCTAATGTTTCCAGCAAAATAAAATGGTATTCGCCGAGACGACGTGTTTCACCCGTTTCGCAATGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTc                                                                                                                                                                                                                                       <  1:81614/298‑1 (MQ=255)                                                        aTGCGGTTCTTCCATACTTCGGCACATAAATAAGATCGCGATAAAACCACATCGGCATTTTTTGCTAATGTTTCCAGCAAAATAAAATGGTATTCGCCGAGACGACGTGTTTCACCCGTTTCGCAATGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCatat                                                                                                                                                                                                                                   >  1:201464/1‑298 (MQ=255)                                                                     aTACTTCGGCACATAAATAAGATCGCGATAAAACCACATCGGCATTTTTTGCTAATGTTTCCAGCAAAATAAAATGGTATTCGCCGAGACGACGTGTTTCACCCGTTTCGCAATGTATTATGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGttt                                                                                                                                                                                                                      <  1:356148/298‑1 (MQ=255)                                                                                                                                                ggTATTCGCCGAGACGACGTGTTTCACCCGTTTCGCAATGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGccc                                                                                                                                           <  1:320747/298‑1 (MQ=255)                                                                                                                                                           agaCGACGTGTTTCACCCGTTTCGCAATGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAATAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTGAGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCTCAGACTTCCACt                                                                                                                                >  2:109283/1‑298 (MQ=255)                                                                                                                                                             acgacgTGTTTCACCCGTTTCGCAATGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTa                                                                                                                              <  1:264414/298‑1 (MQ=255)                                                                                                                                                                  gtgtTTCACCCGTTTCGCAATGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAACg                                                                                                                         >  1:242794/1‑298 (MQ=255)                                                                                                                                                                        aagccatttgtcattGTATTAAGGGAGTTAACGAGGGGTTCATTCGCCCGTGTTTGAGAAAAATACTCTGATTTTAGTTGACCGTAGGAAGGGATACATGTTTCCTGATAGTGCTTTACACTTATGAATTCCATAATGATTTTGGTAAAAATCATTAAGTTAAGATGGATACACATCTTTTCATATGAGCAAATGATTTATCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTAACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAACGACTCaa                                                                                                                   <  2:325034/284‑1 (MQ=14)                                                                                                                                                                              tttCGCAATGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAACGACTCAACAGCtt                                                                                                             <  1:243542/298‑1 (MQ=255)                                                                                                                                                                                    aaTGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATATTAATACTCATAATTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATAATTAAGTTACGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGTTAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTTTGCCCATACTTTAACTTAAAACGACACaaaacatgaacttt                                                                                                       >  2:179595/1‑286 (MQ=21)                                                                                                                                                                                     aTGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTCAGAACTCGATATTTTACCAGAATATCTTTACCAATTCTGCCCCGAATTAAATTTAAAACAACTCAACACCTTAACCTTg                                                                                                      >  2:374947/1‑298 (MQ=21)                                                                                                                                                                                                        gACGGGCCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAACGACTCAACAGCTTAACGTTGGCTTGCCACGCATTACTTg                                                                                   >  2:68878/1‑298 (MQ=255)                                                                                                                                                                                                                        aGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATTAATCCCCTAATGATTTTGGTCAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAACATCAATAATCAGCCAACACGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAACGACTCAACAGTTTAACGTTAGCTGCCCCCGCATTACTAGACTGTAAACctctacc                                                                   >  2:335584/1‑295 (MQ=17)                                                                                                                                                                                                                                                ttGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAACGACTCAACAGCt                                                                                                              >  2:193228/1‑231 (MQ=255)                                                                                                                                                                                                                                                ttGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAACGACTCAACAGCt                                                                                                              <  1:193228/231‑1 (MQ=255)                                                                                                                                                                                                                                                        aCCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAACGACTCAACAGCTTAACGTTGGCTTGCCACGCATTACTTGACTGTaaaa                                                                          <  1:418303/259‑1 (MQ=255)                                                                                                                                                                                                                                                        aCCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAACGACTCAACAGCTTAACGTTGGCTTGCCACGCATTACTTGACTGTaaaa                                                                          >  2:418303/1‑259 (MQ=255)                                                                                                                                                                                                                                                        aCCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAACGACTCAACAGCTTAACGTTGGCTTGCCACGCATTACTTGACTGTAAAACTCTCACTCTTACCGAACTTGGCCGTAACCTGccaacca                                   >  1:38143/1‑298 (MQ=255)                                                                                                                                                                                                                                                         ccTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAACGACTCAACAGCTTAACGTTGGCTTGCCACGCATTACTTGACTGTAAAACTCTCACTCTTACCGAACTTGGCCGTAACCTGccaaccaa                                  >  1:212939/1‑298 (MQ=255)                                                                                                                                                                                                                                                          cTTAGCAAGGGATAAATGTTTCCTGATAGTGCTCTGCACTGATGAATCCGCTAATGAGTTTGGTAAAAATCATTAAGTTAAGATGCATACACATCTTGTCATATGATCAAATGGTTTAGCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAAAGACTAAACAGCTTAACGTTGGCTTGCAACGCATTACTTCACTTTAAAACTCTCCCCCTTACGCAACCTGGACGTAACCTTCACACCaaa                                 >  2:359232/1‑298 (MQ=14)                                                                                                                                                                                                                                                               cAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAACGACTCAACAGCTTAACGTTGGCTTGCCACGCATTACTTGACTGTAAAACTCTCACTCTTACCGAACTTGGCCGTAACCTGCCAACCAAAGCgag                            >  1:404299/1‑298 (MQ=40)                                                                                                                                                                                                                                                                      tAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAACAATCATTAAGTTAAGGTGTATACACATCTTGTCATATGATCACATGGTTTCGCGAAAACTCAATAATACGATAACAAGATGTGCGAACTCGATATTTTACACGACTCTATTTACCAATTCTGCCCAGACTTACACTTAAAACGCCTCAACAGCTTAACGTTGGCTTGCCACGCACTACTTGCCTGTCAAACTATCACTCTTACCGCACTTGCACGTAACCTTcaaaccaagcctcaaacacaa                     >  2:38372/1‑285 (MQ=11)                                                                                                                                                                                                                                                                               ccagATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTTGTAAAAATCATTAAGTCAAGGTGGATACACAACGTGACATATGATCAAATGGTATCGCAAAAAATCAATAATCAGATAACAAGATATGCGAACTCGATCTTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTAAACTTAAAACGAATCAACCGCTAAACGTTCGCTTGCCACGCATTACATGACTTAAAAACTCACTCTCTCACCGAACATGACCGAAAACTGACAACCAAATCCTAAACAAaacacaaactc            >  2:205892/4‑294 (MQ=11)                                                                                                                                                                                                                                                                                      gTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCCTTAAGTTAAGGTAGATACACATCTTGTCATATGATCACATGGTTTCGCGAAAAATCACTAATCAGACAACAAGATGTGCGAACTCGATCTTTTACCCGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAACCGACTCACCATCTTAATGTTGGCTTGCAACGCATTACATCACTGTAAAACTCTAACCCTTGCCGAACTTGGCCATAACCTCCCAACCACACCGAAAACAAAACATAACAAcaaacaaa     >  2:278596/1‑298 (MQ=11)                                                                                                                                                                                                                                                                                         cTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAACGACTCAACAGCTTAACGTTGGCTTGCCACGCATTACTTGACTGTAAAACTCTCACTCTTACCGAACTTGGCCGTAACCTGCCAACCAAAGCGAGAACAAAACATAACATCAAACGAATCg  >  1:252854/1‑298 (MQ=31)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                   ATCGATAGCAACGCGCAACGCATGGATCGCCGTCGGCAGGCTGTTTCCGCCGACAATGCGGTTCTTCCATACTTCGGCACATAAATAAGATCGCGATAAAACCACATCGGCATTTTTTGCTAATGTTTCCAGCAAAATAAAATGGTATTCGCCGAGACGACGTGTTTCACCCGTTTCGCAATGTATTAAGGCATTAAGCGACGGGTCCATCCGCCAGTTGTTGATAATAATACTCATAGTTGAGTTTACCTTAGCAAGGGATAAATGTTTCCTGATAGTGCTTTGCACTGATGAATCCCCTAATGATTTTGGTAAAAATCATTAAGTTAAGGTGGATACACATCTTGTCATATGATCAAATGGTTTCGCGAAAAATCAATAATCAGACAACAAGATGTGCGAACTCGATATTTTACACGACTCTCTTTACCAATTCTGCCCCGAATTACACTTAAAACGACTCAACAGCTTAACGTTGGCTTGCCACGCATTACTTGACTGTAAAACTCTCACTCTTACCGAACTTGGCCGTAACCTGCCAACCAAAGCGAGAACAAAACATAACATCAAACGAATCG  >  CP006584/345781‑346358

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.