Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	1,324,434	A→C	K833T (AAA→ACA)	narZ →	nitrate reductase A subunit alpha

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	1,324,434	0	A	C	100.0%	71.9 / NA	22	K833T (AAA→ACA)	narZ	nitrate reductase A subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (13/9);  total (13/9)

CTGGGGCAACAGGGCGGCGTGAAGCCGGAAGA‑AGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATG  >  CP006584/1324166‑1324711                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                      ggggggtggaggggCGGCGGGAAGGCGGAAGAGAGTGG‑GTGGCAGGACAATGGTTTGGAGGGGTAGCTGGTGCTGGTGGTTACGCTGGACTGCCGGCTGTCGCGGCGCTTGCTGTATTCCGACATCATTTGGCCGGAGGCGAGCTGGTACGAAAATGACTACATGAATACTTCGGGTATGCATGCGTTTATTCACCCGCTGGCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTgg                                                                                                                                                                                                                                                          <  2:320307/287‑1 (MQ=255)          cAGGGCGGCGTGAAGCCGGAAGA‑AGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGGAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGaaa                                                                                                                                                                                                                                                 >  1:6661/1‑298 (MQ=255)                          cGGAAGA‑AGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACCTTCTCCGAAGTGTGCGTCGGTCATCTGGGAAAAGCAACCGACCTCGTCAcccc                                                                                                                                                                                                                                 >  2:386627/1‑295 (MQ=255)                                  agTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATc                                                                                                                                                                                                                          >  1:210173/1‑298 (MQ=255)                                                          ggAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATCTGCATCCGTTTACTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGAACGGGAAATGTACAAAGCCATCGCGAAGACATTCTCCGAAGCGTGCGTCGGTCACCCGGGTAAGGAAACCGACATCGTCAGTCTGCCTCTCCCGCCCGACCCAGccccaggaacg                                                                                                                                                                                                  >  2:332543/1‑289 (MQ=255)                                                                        aTCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGa                                                                                                                                                                                    <  1:175935/298‑1 (MQ=255)                                                                                    cGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTg                                                                                                                                                                        <  1:386627/298‑1 (MQ=255)                                                                                                        aGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAACAAGACGACATGAATACTTCTGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCGCCGAACTGTGCGTCGGTCATCTGGGTAAAGCAACCGACATCGTCACGCTGCCTCTCCAGCACGACTCTGCCGCTGAACTGGCGCATCCGCTCGATGTCACAGACCGGAAACAAGGccaatcacacc                                                                                                                                                    >  2:367472/1‑288 (MQ=255)                                                                                                            ccTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGAt                                                                                                                                                >  1:112946/1‑298 (MQ=255)                                                                                                             cTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGCTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGCAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCCTCACCCTGCCTATCCACCACGACCCTGCCGCTGCACTGGCGCAGCCGCTGGATTCGGAAGCCTGGAAAAACGGGCAGCGCGAccagcac                                                                                                                                               >  2:162639/1‑293 (MQ=255)                                                                                                                tctcTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCt                                                                                                                                            >  1:361448/1‑298 (MQ=255)                                                                                                                       tCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTaaaa                                                                                                                                     <  1:162378/298‑1 (MQ=255)                                                                                                                                 ttttGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCcaca                                                                                                                           <  1:367851/298‑1 (MQ=255)                                                                                                                                                ccTGGTACGAAAAAGACGACATGAAGACTTCGGTGATGCATCCGTTTATTCACCCGCGGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTaga                                                                                                            <  2:210173/298‑1 (MQ=255)                                                                                                                                                                            ttCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGc                                                                                >  1:338013/1‑298 (MQ=255)                                                                                                                                                                                       aTCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTAt                                                                     <  1:322584/298‑1 (MQ=255)                                                                                                                                                                                                     ccGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAAATTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATgg                                                       >  1:184087/1‑298 (MQ=255)                                                                                                                                                                                                                     tCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGc                                                             <  1:116511/276‑1 (MQ=255)                                                                                                                                                                                                                     tCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCCCTATCGGCCCGc                                                             >  2:116511/1‑276 (MQ=255)                                                                                                                                                                                                                          ccGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTa                                  >  1:299557/1‑298 (MQ=255)                                                                                                                                                                                                                                         aaaaGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGa                   >  1:321525/1‑298 (MQ=255)                                                                                                                                                                                                                                                          ttACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATg  <  1:332543/298‑1 (MQ=255)                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                      CTGGGGCAACAGGGCGGCGTGAAGCCGGAAGA‑AGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTCTATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAACCGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATG  >  CP006584/1324166‑1324711

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑