Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	1,464,821	A→G	intergenic (+486/‑118)	LY180_06955 → / → LY180_06960	hypothetical protein/regulatory protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	1,464,821	0	A	G	100.0%	94.8 / NA	29	intergenic (+486/‑118)	LY180_06955/LY180_06960	hypothetical protein/regulatory protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (15/14);  total (15/14)

TACAGAACAAAATCACAGAAACTATACCCAATAGTTGCATTGAATCACTGACGAGACATCCTCATATTTATCAGGACTGGTGTACGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCAGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATTGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGC  >  CP006584/1464561‑1465090                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                              gtgctcttccgaTCTCAGAAACTATACCCAATAGTTGCATTGAATCACTGACGAGACTTCCTCATATTTATCAGGACTGGTGTACGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGTGTGCTTGATGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCa                                                                                                                                                                                                                                          <  1:211410/287‑1 (MQ=255)     gAACAAAATCTCAGAAACTATACCCAATAGTTGCATTGAATCACTGACGAGACATCCTCATATTTATCAGGACTGGTGTACGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTa                                                                                                                                                                                                                                      >  1:264468/1‑298 (MQ=255)                caGACACTATACCCAATAGTTGCATTGAATCCCTGACGAGACATCCTCATATTTATCAGGACTGGTGTACGTCCAATACAGGAGGCTGCCGTGCTGGCTCTCAAATGTGCGCTAGCTATTGCGGATGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCCTTGTGATTGATTTTGTATTGTCGAGGTGAAGGGGTTTAGGTATAATTGATGCTGATGATTGGGATCATCTGTCTCGCGCGTACCCACGAAACGCAATGAAGAAAAAAGCCACAGTTAACagtgtgcat                                                                                                                                                                                                                           >  2:22146/1‑290 (MQ=255)                     aCTATACCCAATAGTTGCATTGAATCACTGACGAGACATCCTCATATTTATCAGGACTGGTGTACGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGc                                                                                                                                                                                                                      >  1:318031/1‑298 (MQ=255)                         taCCCAATAGTTGCATTGAATCACTGACGAGACATCCTCATATTTATCAGGACTGGTGTACGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGa                                                                                                                                                                                                                  <  1:283159/298‑1 (MQ=255)                                 aGTTGCTTTGAACCCCTGACGAGACATCCTCATATTTATGAGGACTGGTGTACGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAAc                                                                                                                                                                                                          <  2:209944/298‑1 (MQ=255)                                           aaTCACTGACGAGACATCCTCATATTTATCAGGACTGGTGTACGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGa                                                                                                                                                                                                >  1:172081/1‑298 (MQ=255)                                                                      aTCAGGACTGGTGTACGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCACTTTATTGTCTTTCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGCGGTGCCGGAGGTTAAGTAAACTTTCTGCGGGTGCTTGAGGCTCTCTGTCTCGGGCATGAACACCAAAAGTCAGATAGAGAAACGCCCCCGTTCACATTACGCGTCTGGCCAGACGCGTAAACTTCAACTGAGCCCCTCTCTCTGCCCCCCATAGAGAt                                                                                                                                                                     >  2:251112/1‑298 (MQ=255)                                                                           gaatGGTGTACGTCAAATACAGGAGGTTGTTGGGCTTGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCGGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGATTGAGGCTATCTGTCTCGTGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGcc                                                                                                                                                                <  2:267858/295‑1 (MQ=255)                                                                                    aCGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTAtgtg                                                                                                                                                       <  1:350843/298‑1 (MQ=255)                                                                                       tCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATCTATTGTCTTGCTGTTGTTCTTATGGATCGCC‑TTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTATCGGTAATGAACACCCAAAGGCAGCTGGAGAACAGCCCCCGTTAAACTTACGCGTCCGGCACGACGATTAACACTAATCTGAGGACATATCTAAGCCACACCTAGAGATTATCCTCATATGTTccta                                                                                                                                                   >  2:370415/1‑296 (MQ=255)                                                                                                ggaggTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGACAAg                                                                                                                                           >  1:327031/1‑298 (MQ=255)                                                                                                          gTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAgg                                                                                                                                 >  1:228443/1‑298 (MQ=255)                                                                                                                 ttCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGaa                                                                                                                          >  1:144303/1‑298 (MQ=255)                                                                                                                            gcgcTATCTATTGCGGTTGTAATTGGAATTTATTGTTTTGTTTTTGTTCTTATGGATAGTCTTTCTGGTTGAGTTTATATTGTGGCGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGACGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAgg                                                                                                               <  2:422351/298‑1 (MQ=25)                                                                                                                                      ttGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTAAt                                                                                                     >  1:56760/1‑298 (MQ=255)                                                                                                                                      ttGAGGCTTTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATTGCCTTTCTGATTTATTTTATATTGGCGAGGTGAGGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGACGCAGGCTATGAAGCAGCAAAAGGCGATGTTAAt                                                                                                     <  1:285363/298‑1 (MQ=255)                                                                                                                                          ggCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTAATCGcc                                                                                                 >  1:300861/1‑298 (MQ=255)                                                                                                                                              gTAATGGCAATTTATTGTCTTGCTGTAGTTCTTATGGATCGTCTGTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGTGTGCTGTAGGTTATCTGTCTCGGGCATGAACACCAAACGGTAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGGTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAATGAGAAGCAGGATATTAAGCAGCAAAAGGCGATGTTAATCGCCCTGa                                                                                             <  1:110100/298‑1 (MQ=37)                                                                                                                                               tAATGGCAATTTTTTTTTTTGCGGTTGTTCTTTTGGTTGGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGTGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGAt                                                                                            <  2:172081/298‑1 (MQ=255)                                                                                                                                                               tCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAAcc                                                                            >  1:354271/1‑298 (MQ=255)                                                                                                                                                                                   aTCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATTGTGACGGCACTGGt                                                        >  1:194256/1‑298 (MQ=255)                                                                                                                                                                                     cGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGTTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATTGTGACGGCACTGGTaa                                                      <  1:22146/298‑1 (MQ=255)                                                                                                                                                                                      gctttttttattgtttTTATTGTGGCGGGTTGCGTGGGTTTAAATAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGCACACCAAAAGGCAGAAAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATTGTGACGGCACTGGTAAc                                                     <  2:418044/289‑1 (MQ=38)                                                                                                                                                                                             tgattgatTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATTGTGACGGCACTGGTAACGAGGaaa                                              <  1:63701/298‑1 (MQ=255)                                                                                                                                                                                                ttgattTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGTGACACATAGAGATTAGCCTATTATGTGCCGAACGGCACGGAGAAGCAGGCTATGAAGCATCAAAATGCGTGGTTAACCGGCCTGCTCGTCATCTGTTTTACCTGCATTTTTGCGGCATCGTGTACCAGGAAAGAc                                           >  2:98383/1‑298 (MQ=21)                                                                                                                                                                                                       tataTTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATg                                                                                                                            >  2:161997/1‑210 (MQ=255)                                                                                                                                                                                                       tataTTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATg                                                                                                                            <  1:161997/210‑1 (MQ=255)                                                                                                                                                                                                                                         gctgcGGGTGCTTGAGGCTATCTGTCTCGGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATTGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGc  <  1:413322/298‑1 (MQ=255)                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                              TACAGAACAAAATCACAGAAACTATACCCAATAGTTGCATTGAATCACTGACGAGACATCCTCATATTTATCAGGACTGGTGTACGTCCAATACAGGAGGTTGTCGTGCTGGTTCTCAAATGTGCGCTAGCTATTGCGGCTGTAATGGCAATTTATTGTCTTGCTGTTGTTCTTATGGATCGCCTTTCTGATTGATTTTATATTGGCGAGGTGACGGGGGTTAAGTAGAATTGCTGCGGGTGCTTGAGGCTATCTGTCTCAGGCATGAACACCAAAAGGCAGATAGAGAAAAGCCCCAGTTAACATTACGCGTCCGGCAAGACGCTTAACATTAATCTGAGGCCATATCTATGCGACACATAGAGATTAGCCTCTTATGTGCCGAAAGGCAAGGAGAAGCAGGCTATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATTGTGACGGCACTGGTAACGAGGAAAGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGC  >  CP006584/1464561‑1465090

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑