Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	2,265,396	A→G	intergenic (‑149/‑25)	LY180_10855 ← / → LY180_10860	precorrin‑8W decarboxylase/terminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	2,265,396	0	A	G	100.0%	19.7 / NA	15	intergenic (‑149/‑25)	LY180_10855/LY180_10860	precorrin‑8W decarboxylase/terminase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (5/10);  total (5/10)

CATAGACTCGCGGGTCAAAGGTTTCGGCCATTTCCTGAATATCCTGCGCACTGATGACACGCCCGTCACAGGTGTCACCCTCAACGCCGATACGAAAGAATTTTGAGACTTTTTTTGCCATTGTCAGGAGTCCTGAATAGTGATTAGAGGAGTCACATGTCGGCATCAGTTTCCCGACGATGCGCATCCTCCGCCATCAGTCCCGGATGGCTTATCACTGGCACAACAGCACCTTAGCGAATCGCGGGGCGCGACTCAGTAGCCTTGCCATGGATTCATCACGGCGAGGTATTCATGACCATCACCACAGACACCACTCTTTTACACGACCCGCGTCGTCAGGCGGCGCTGCTGTACTGGCAGGGATTTTCCGTGCCGCAGATTGCCGCCATGTTGCAGATGAAACGCCCGACGGTGCAGAGCTGGAAACAGCGCG‑ACGGCTGGGACAGCGTTGCCCCCATCAGCCGTGTCGAAATGAGTCTGGAAGCGCGGCTGACCCAGCTCATCATCAAACCGCAGAAAACCGGCGGTGACTTCAAGGAAATTGACCTGC  >  CP006584/2265127‑2265679                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                             ataggagtCGCGGGTAAAAGGTTTGGGCCATTTTCTGAATATCCTGCGCACTGATGACACGCCCGTCCCAGGTGTAACCCTCAACGCGGATACGAAAGAATTTTGCGACTTGTTTTGCCATTGTCAGGAGTCCTGACTAGTGATGAGAGGAGTAACATGTCGGCATCAGTTTCCCGACGATGCGCATCCTCCGCCATCAGTCCCGGATGGCTTATCACTGACACAACAGCACCTTAGCGAATCGCGGGGCGCGACTCAGTAGCCTTGCCGTGTATTCATCACGGCGAGGTATTCATGa                                                                                                                                                                                                                                                                  <  2:28435/291‑1 (MQ=1)                                                                                        cGATACGAAAGAATTTTGAGACTTTTTTTGCCATTGTCAGGAGTCCTGAATAGTGATTAGAGGAGTCACATGTCGGCATCAGTTTCCCGACGATGCGCATCCTCCGCCATCAGTCCCAGATGGCTTATCACTGACACAACAGCACCTTAGCGAATCGCGGGGCGCGACTCAGTAGCCTTGCCGTGTATTCATCACGGCGAGGTATTCATGACCATCACCACAGACACCACTCTTTTACACGACCCGCGTCGTCCGCCCGCCCTGCTGTCCTGGCAGGGATTTTCCGTGCCGCCGGTTg                                                                                                                                                                           >  2:149886/1‑298 (MQ=11)                                                                                                               ttttttGCATTTGTACGGTGGCCTGAATAGTGATTAGAGGTGTCAAATTTCGTAATCAGTTTCCCGACGATGCGCATCCTCCGCCATCGGTCCCGGATGGCTTATCACTGACCCAACGGCACCTTAGCGAATCGCGGGGCGCGACTCAGTAGCCTTGCCGTGTATTCATCACGGCGAGGTATTCATGACCATCACCACAGACACCACTCTTTTACACGACCCGCGTCGTCAGGCGGCGCTGCTGTACTGGCAGGGATTTTCCGTGCCGCAGATTGCCGCCATGTTGCAGATGAAACGc                                                                                                                                                    <  2:5521/298‑1 (MQ=11)                                                                                                                                gAGTCCTGAATAGTGATTAGAGGAGTCACATGTCGGCACCAGTTTCCCGACGATGCGCATCCTCCGCCATCAGTCCCGGATGGCTTATCACTGACACAACAGCACCTTAGCGAATCGCGGGGCGCGACTCAGTAGCCTTGCCGTGTATTCATCACGGCGAGGTATTCATGACCATCACCACAGACACCACTCTTTTACACGACCCGCGTCGTCAGGCGGCGCTGCTGTACTGGCAGGGATTTTCCGTGCCGCAGATTGCCGCCATGTTGCAGATGAAACGCCCGACGGTGCAGAGCTg                                                                                                                                   <  1:31038/298‑1 (MQ=14)                                                                                                                                               aTTAGAGGAGTCACATGTCGGCATCAGTTTCCCGACGATGCGCATCCTCCGCCATCAGTCCCGGATGGCTTATCACTGACACAACAGCACCTTAGCGAATCGCGGGGCGCGACTCAGTAGCCTTGCCGTGTATTCATCACGGCGAGGTATTCATGACCATCACCACAGACACCACTCTTTTACACGACCCGCGTCGTCAGGCGGCGCTGCTGTACTGGCAGGGATTTTCCGTGc                                                                                                                                                                                    <  1:228717/234‑1 (MQ=14)                                                                                                                                               aTTAGAGGAGTCACATGTCGGCATCAGTTTCCCGACGATGCGCATCCTCCGCCATCAGTCCCGGATGGCTTATCACTGACACAACAGCACCTTAGCGAATCGCGGGGCGCGACTCAGTAGCCTTGCCGTGTATTCATCACGGCGAGGTATTCATGACCATCACCACAGACACCACTCTTTTACACGACCCGCGTCGTCAGGCGGCGCTGCTGTACTGGCAGGGATTTTCCGTGc                                                                                                                                                                                    >  2:228717/1‑234 (MQ=14)                                                                                                                                                      gagGCACAGGCCGGCAACAGTGTCCCGCCGCTGCCCACCCTCCGCCATCAGTCCCGGATGGCTTATCACTGACACAACAGCACCTTAGCGAAGCGCGGGGCGCGACTCAGTAGCCTTGCCGTGTATTCATCACGGCGAGGTATTCATGACCATCACCACAGACACCACTCTTTTACACGACCCGCGTCGTCAGGCGGCGCTGCTGTACTGGCAGGGATTTTCCGTGCCGCAGATTGCCGCCATGTTGCAGATGAAACGCCCGACGGTGCAGAGCTGGAAACAGCGCG‑ACGGCTGGGAc                                                                                                            <  2:37448/298‑1 (MQ=11)                                                                                                                                                            caTGTCGGCATCAGTTTCCCGACGATGCGCATCCTCCGCCATCAGTCCCGGATGGCTTATCACTGACACAACAGCACCTTAGCGAATCGCGGGGCGCGACTCAGTAGCCTTGCCGTGTATTCATCACGGCGAGGTATTCATGACCATCACCACAGACACCACTCTTTTACACGACCCGCGTCGTCAGGCGGCGCTGCTGTACTGGCAGGGATTTTCCGTGCCGCAGATTGCCGCCATGTTGCAGATGAAACGCCCGACGGTGCAGAGCTGGAAACAGCGCG‑ACGGCTGGGACAGCGtt                                                                                                      >  1:141992/1‑298 (MQ=14)                                                                                                                                                                                             ctccGCCATCAGTCCCGGATGGCTTATCACTGACACAACAGCACCTTAGCGAATCGCGGGGCGCGACTCAGTAGCCTTGCCGTGTATTCATCACGGCGAGGTATTCATGACCATCACCACAGACACCACTCTTTTACCCGACCCGCGTCGTCAGGCGGCGCTGCTGTACTGGCAGGGATTTTCCGTGCCGCAGATTGCCGCCATGTTGCAGATGAAACGCCCGACGGTGCAGAGCTGGAAACAGCGCG‑ACGGCTGGGACAGCGTTGCCCCCATCAGCCGTGTCGAAATGAGTCTGGaa                                                                     <  1:156665/298‑1 (MQ=14)                                                                                                                                                                                                 gCCATCAGTCCCGGATGGCTTATCACTGACACAACAGCACCTTAGCGAATCGCGGGGCGCGACTCAGTAGCCTTGCCGTGTATTCATCACGGCGAGGTATTCATGACCATCACCACAGACACCACTCTTTTACACGACCCGCGTCGTCAGGCGGCGCTGCTGTACTGGCAGGGATTTTCCGTGCCGCAGATTGCCGCCATGTTGCAGATGAAACGCCCGACGGTGCAGAGCTGGAAACAGCGCG‑ACGGCTGGGACAGCGTTGCCCCCATCAGCCGTGTCGAAATGAGTCTGGAAgcgc                                                                 <  1:17130/298‑1 (MQ=14)                                                                                                                                                                                                        gTCCCAGATGGCTTATCACTGACACAACAGCACCTTAGCGAATCGCGGGGCGCGACTCAGTAGCCTTGCCGTGTATTCATCACGGCGAGGTATTCATGACCATCACCACAGACACCACTCTTTTACACGACCCGCGTCGTCAGGCGGCGCTGCTGTACTGGCAGGGATTTTCCGTGCCGCAGATTGCCGCCATGTTGCAGATGAAACGCCCGACGGTGCAGAGCTGGAAACAGCGCG‑ACGGCTGGGACAGCGTTGCCCCCATCAGCCGTGTCGAAATGAGTCTGGAAGCGCGGCTGAc                                                          <  1:149886/298‑1 (MQ=14)                                                                                                                                                                                                                                   caGCACCTTAGCGAATCGCGAGGCGCGACTCAGTAGCCTTGCCGTGTATTCATCACGGCGAGGTATTCATGACCATCACCACAGACACCACTCTTTTACACGACCCGCGTCGTCAGGCGGCGCTGCTGTACTGGCAGGGATTTTCCGTGCCGCAGATTGCCGCCATGTTGCAGATGAAACGCCCGACGGTGCAGAGCTGGAAACAGCGCG‑ACGGCTGGGACAGCGTTGccccc                                                                                                >  1:171230/1‑233 (MQ=14)                                                                                                                                                                                                                                   caGCACCTTAGCGAATCGCGAGGCGCGACTCAGTAGCCTTGCCGTGTATTCATCACGGCGAGGTATTCATGACCATCACCACAGACACCACTCTTTTACACGACCCGCGTCGTCAGGCGGCGCTGCTGTACTGGCAGGGATTTTCCGTGCCGCAGATTGCCGCCATGTTGCAGATGAAACGCCCGACGGTGCAGAGCTGGAAACAGCGCG‑ACGGCTGGGACAGCGTTGccccc                                                                                                <  2:171230/233‑1 (MQ=14)                                                                                                                                                                                                                                                aaTCGCGGGGCGCGACTCAGTAGCCTTGCCGTGTATTCATCACGGCGAGGTATTCATGACCATCACCACAGACACCACTCTTTTACACGACCAGCGTCGACAGGCGGCGCTGCTGTACTGGCAGGGATTTTCCGTGCCGCACATAGCCGCCATGTTTCAGATGACAAGCCCGACGGTACAGATCTGGAAACCG‑GCGAACGACCGGGACAGCTGTCCCCCCACCCGCCGGTTCACAATGGGGCTGGAAGCGCACCTGCCCCGGCGCAACAGTACACCACAGAACAccagggtgatcgcc                  >  2:10922/1‑286 (MQ=2)                                                                                                                                                                                                                                                                tCAGTAGCCTTGCCGTGTATTCATCACGGCGAGGTATTCATGACCATCACCACAGACACCACTCTTTTACACGACCCGCGTCGTCAGGCGGCGCTGCTGTACTGGCAGGGATTTTCCGTGCCGCAGATTGCCGCCATGTTGCAGATGAAACGCCCGACGGTGCAGAGCTGGAAACAGCGCG‑ACGGCTGGGACAGCGTTGCCCCCATCAGCCGTGTCGAAATGAGTCTGGAAGCGCGGCTGACCCAGCTCATCATCAAACCGCAGAAAACCGGCGGTGACTTCAAGGAAATTGACCTGc  <  1:47289/298‑1 (MQ=14)                                                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                                                             CATAGACTCGCGGGTCAAAGGTTTCGGCCATTTCCTGAATATCCTGCGCACTGATGACACGCCCGTCACAGGTGTCACCCTCAACGCCGATACGAAAGAATTTTGAGACTTTTTTTGCCATTGTCAGGAGTCCTGAATAGTGATTAGAGGAGTCACATGTCGGCATCAGTTTCCCGACGATGCGCATCCTCCGCCATCAGTCCCGGATGGCTTATCACTGGCACAACAGCACCTTAGCGAATCGCGGGGCGCGACTCAGTAGCCTTGCCATGGATTCATCACGGCGAGGTATTCATGACCATCACCACAGACACCACTCTTTTACACGACCCGCGTCGTCAGGCGGCGCTGCTGTACTGGCAGGGATTTTCCGTGCCGCAGATTGCCGCCATGTTGCAGATGAAACGCCCGACGGTGCAGAGCTGGAAACAGCGCG‑ACGGCTGGGACAGCGTTGCCCCCATCAGCCGTGTCGAAATGAGTCTGGAAGCGCGGCTGACCCAGCTCATCATCAAACCGCAGAAAACCGGCGGTGACTTCAAGGAAATTGACCTGC  >  CP006584/2265127‑2265679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑