Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	3,278,551	G→A	W246* (TGG→TGA)	LY180_15575 →	aldehyde oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	3,278,551	0	G	A	94.9%	119.1 / NA	39	W246* (TGG→TGA)	LY180_15575	aldehyde oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (19/18);  total (19/20)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGGGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCT  >  CP006584/3278265‑3278843                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                     tGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCa                                                                                                                                                                                                                                                                                           >  1:172188/1‑298 (MQ=255)   ctctcCCGTAAAACCACCGGGGGCAAGCGGGCGGTCCTGTCTGCCCGTTTTCGGCCGGGGGTTGCCGTGCTCGATCCGGGTTATACCTACACCCTGCCGCCGCGTCAGGTGGGTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAgg                                                                                                                                                                                                                                                                                         <  2:47733/297‑1 (MQ=255)      tccgatctaaCCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGgcgc                                                                                                                                                                                                                                                                                      <  1:272007/290‑1 (MQ=255)           tAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAAc                                                                                                                                                                                                                                                                                 <  1:193390/298‑1 (MQ=255)               aCCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTGGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAATATGGTCGGAAAGCCCTGAAAAAGCCAGAAAACTACGTCGTGCGCGCCACCGTCATGTGAGGGGCGAGGCCGgcgccagatggga                                                                                                                                                                                                                                                                             >  2:272007/1‑289 (MQ=255)                           aaGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTg                                                                                                                                                                                                                                                                 >  1:378668/1‑298 (MQ=255)                           aaGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTg                                                                                                                                                                                                                                                                 <  1:42413/298‑1 (MQ=255)                               aGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGt                                                                                                                                                                                                                                                             >  1:194051/1‑298 (MQ=255)                                 gCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTAc                                                                                                                                                                                                                                                           >  1:388033/1‑298 (MQ=255)                                              cccATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGc                                                                                                                                                                                                                                              >  1:397767/1‑298 (MQ=255)                                                               tttGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGcc                                                                                                                                                                                                                             >  1:87891/1‑298 (MQ=255)                                                                ttGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCa                                                                                                                                                                                                                            <  1:211049/298‑1 (MQ=255)                                                                            gtCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGTCGTGGTGGACGCCTTGGTACACACCGGGGAACAGTTGGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGc                                                                                                                                                                                                                <  2:194051/297‑1 (MQ=255)                                                                                       acccgcccccGGCGGCCGCGGCAGGTGGCTAACGGCGTAGGGGAGGCCTTTGTACAAACCGTGGAACAGTATGGTACCAAACCGGTTGATGCCAAAACTCAGGACCGTTTGGCAGAAGGGATTTTGCTGACGCGGAT‑CGAGGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAGCGACGATGTGCGCGCCAACGTCATGTGGGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTc                                                                                                                                                                                                     <  2:389125/291‑1 (MQ=255)                                                                                                      ccgcGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCaaa                                                                                                                                                                                      <  1:40063/298‑1 (MQ=255)                                                                                                      ccgcGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCACCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCaaa                                                                                                                                                                                      >  1:171232/1‑298 (MQ=255)                                                                                                               gTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTAGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTa                                                                                                                                                                             <  1:334864/298‑1 (MQ=255)                                                                                                                 ggCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATc                                                                                                                                                                           >  1:290963/1‑298 (MQ=255)                                                                                                                  gCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTATCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCg                                                                                                                                                                          <  1:356135/298‑1 (MQ=255)                                                                                                                   cTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTGGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCTATGCGCGGCCCGGAACACGCCCaacacccggccaccct                                                                                                                                                                         >  2:136808/1‑284 (MQ=255)                                                                                                                         gCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTcctgcc                                                                                                                                                                   <  1:120785/298‑1 (MQ=255)                                                                                                                                ggACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTATGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACtg                                                                                                                                                            <  1:77304/298‑1 (MQ=255)                                                                                                                                                   gTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCACTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCAGGAAAGAGCCCGAAAACTACGATGTCCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGCACGTTTTGATTGGCGCTGGCCTACCGACGGACTGGGCAACGCATCTTCGGGGCCGCGAACTGACTGCAGTGCACGGTCTGGACCAAGCGCCAACACTTGCTCACCTCCTCCCTTCACTGTGGAATGCAACAcgcgcca                                                                                                                                         >  2:413516/1‑295 (MQ=255)                                                                                                                                                         cAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGCTCACGCGCAAACACTGGCTATCGTCCTGCCTGCAAGGTGGGATGGAAAACGCGAGAcccacc                                                                                                                                   >  2:178321/1‑294 (MQ=255)                                                                                                                                                             aTGTTCCCAAGCCGGTTGATGCCAAAATTACGGCCCGGTTCGCAGAAGGCATTTTGCTGAGGCTGAT‑CGAAGATGGTCCGAAAGCCCGGAAAGAGCAAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGAGTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAgcgcgc                                                                                                                               <  2:290963/298‑1 (MQ=255)                                                                                                                                                              tGTTACCAAACCGGTTGATGCCAAAATTCCGGCCCGTTTCGCAGAAGGCATTTTGCGGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAACCTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACTAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCt                                                                                                                              <  1:413516/298‑1 (MQ=255)                                                                                                                                                                     aaaCCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCCGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGCATGAAAAACGCGAGACCAAGCGCCCTAAgcgcc                                                                                                                       >  2:13497/1‑295 (MQ=255)                                                                                                                                                                                    aaaaTTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAAc                                                                                                        >  1:234144/1‑298 (MQ=255)                                                                                                                                                                                           aGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTg                                                                                                 >  1:298507/1‑298 (MQ=255)                                                                                                                                                                                              agcgtggcggggAAGGTATTTTGGTGAGGCTGAT‑CGAAGATGGTCCGAAAGCCCGGCAAGAGCCAGAAAACTACGATTGGGCCGCCACGGTCATGGTAGGGGCGACGCAGGCGCTGAGCGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGaa                                                                                              <  2:388033/287‑1 (MQ=255)                                                                                                                                                                                                 gTTTCGCGGAGGGCATTTTGGTGACG‑GGATGCAAGGAGGGGCCGAAAGCCGTGGAAGAGCCGGAAAACTGCGATGTGCGCGCAAAGGGCGTGGGGGGGGCGACGCAGGCGGTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACGGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACAt                                                                                           <  2:397767/298‑1 (MQ=255)                                                                                                                                                                                                        aGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGCAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGACTACGCGCAAACACTAGCTAACGTCCTGCCTGCACTGGGGAATGGAAAACGCGAGACCAAGCCGCCCAAGCTGCTGACATATGCTGAACGCGTCCGGAACATccacgaa                                                                                    >  2:53154/1‑292 (MQ=255)                                                                                                                                                                                                          aaGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAgg                                                                                  <  1:13497/298‑1 (MQ=255)                                                                                                                                                                                                            ggCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGtt                                                                                >  1:289985/1‑298 (MQ=255)                                                                                                                                                                                                               aTTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCg                                                                             <  1:164049/298‑1 (MQ=255)                                                                                                                                                                                                                ttttGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCga                                                                            >  1:326310/1‑298 (MQ=255)                                                                                                                                                                                                                             gcggcgagaagggTCGGAAGGCCCTGAAAGGGCCACGAAAGTACGGTGGGCGCGCCAACGTCATGTGAGGGGGGAGGCAGGCGCTGAACGGTGTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTAt                                                                <  2:87891/287‑1 (MQ=255)                                                                                                                                                                                                                                                                         gATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAg                    <  1:178321/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                           aTGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAAAATCCCTGAAGGGTCCGATGAGCAGCGTATTGCCGCCCAGCTTTCCGCACCCCCCAAATTCCTTGACCAATTTGGCGGGCCCAccacccccc  >  2:8205/1‑291 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                     TGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGAT‑CGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGGGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGACCCACCTCT  >  CP006584/3278265‑3278843

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑