Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	3,505,943	C→T	G41D (GGT→GAT)	LY180_16680 ←	malate dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	3,505,943	0	C	T	97.7%	137.8 / NA	44	G41D (GGT→GAT)	LY180_16680	malate dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (22/20);  total (24/20)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01

ACACCGGCTTTTTTCAGCACTTCAGCAGCAATCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAATTGCCGTAATCAGGAACCTAACTTACGTAATTAACA  >  CP006584/3505655‑3506234                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                    acacCGGCTTTTTTCAGCACTTCAGCAGCAATCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCa                                                                                                                                                                                                                                                                                            >  1:362337/1‑298 (MQ=255)                     ttCAGCAGCAATCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACaga                                                                                                                                                                                                                                                                        >  1:351659/1‑298 (MQ=255)                               aTCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCCCGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCCTCCAGCGCCGGAGTCGCATCTTCACAAGCAAAACCTTTCATTTTCACAGCCGTAGGGATATGACCCAGTACGACAGCCACAACGGAGTTCACTGTAGCAAAACCATAAACAGAGAGTGCta                                                                                                                                                                                                                                                              >  2:73257/1‑297 (MQ=255)                               aTCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGACCGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACCGCCACACCGGGAGTCACTGGACCGATATCACACAGAGAGCGTTATg                                                                                                                                                                                                                                                              >  2:58992/1‑298 (MQ=255)                                      cTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGa                                                                                                                                                                                                                                                       <  1:240935/298‑1 (MQ=255)                                          ggtgTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGc                                                                                                                                                                                                                                                   >  1:188811/1‑298 (MQ=255)                                                                 taCCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGc                                                                                                                                                                                                                            >  1:416813/1‑298 (MQ=255)                                                                     catgtccggttgctggCAGGTTTTCCAAAATTGGTGTACCCGGTTTTTCCCGATGGCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTCCGGGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAgc                                                                                                                                                                                                                        <  2:351659/284‑1 (MQ=255)                                                                                   ggCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATAcc                                                                                                                                                                                                          >  1:147446/1‑298 (MQ=255)                                                                                           ttCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCcagcag                                                                                                                                                                                                  <  1:146216/298‑1 (MQ=255)                                                                                                  tttgtgttagccggTTTTGAATGATGTGGGCGTTAACAGTAAAAAGCTCGTGACGTGCCATCCCCGGGTTAAGCGGTATCTCTGCAGAGCAAAGAACTACATCTGCGCCGTCCAGCGCCGGACTCGCATATGCACCAGAAAGAAATTTGATTTTCACAGCAGTAGGGATATGGCGAATATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAg                                                                                                                                                                                           <  2:325678/286‑1 (MQ=255)                                                                                                     gCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGag                                                                                                                                                                                        >  1:198680/1‑297 (MQ=255)                                                                                                        gTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGc                                                                                                                                                                                     <  1:162826/298‑1 (MQ=255)                                                                                                              ggTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCCGTTGGGGTTTTAACCGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTggcccttt                                                                                                                                                                               >  2:396419/1‑291 (MQ=255)                                                                                                               gtgtttAACGATCCCGTGGTTAACAGTAACCAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTc                                                                                                                                                                              <  2:362337/295‑1 (MQ=255)                                                                                                                 ttttCACGATCCGGTCATTAACGTTAAACAGGTCGGGACGTTCCATACCCGGTTTACGGGCTACCCCTGCAGAGATCACAACGTCAGCTGCGCCTTCCAGCGCCGGAGTCGCATCGTCACCAGAAGATGCTTTGATGTGAGCAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGGCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCAt                                                                                                                                                                            <  2:249161/298‑1 (MQ=255)                                                                                                                      aCGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTaa                                                                                                                                                                       >  1:52064/1‑298 (MQ=255)                                                                                                                                            aCAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAAc                                                                                                                                                 >  1:314427/1‑298 (MQ=255)                                                                                                                                            aCAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAAACGTAGTTCAAGCGCATGGCCACTACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAACATCTTTATCATATTTATAAAc                                                                                                                                                 >  2:419797/1‑298 (MQ=255)                                                                                                                                                   ggAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAAAAGTAGTGCAAGCGCCTGGCCAATCACGCCAGCAGCGCCGAGGAATGCGGCGTTCATACTAAACTCCTTATTAATTTGATTaaataaaata                                                                                                                                          >  2:202637/1‑298 (MQ=255)                                                                                                                                                       cGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGtt                                                                                                                                      <  1:58992/298‑1 (MQ=255)                                                                                                                                                       cGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGtt                                                                                                                                      <  1:386645/298‑1 (MQ=255)                                                                                                                                                        gATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTg                                                                                                                                     >  1:320699/1‑298 (MQ=255)                                                                                                                                                         acccATAACCGGTATACGGGCTACACATGCAGAGATAAGAACGACATGTGAGCCTTCCAGCGCCGGAGGCGCATCTTCACCAGAAAAACCTTTGATGTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGc                                                                                                                                    <  2:351523/296‑1 (MQ=255)                                                                                                                                                         aTCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCCGAAGGCAGTTTGGTTTTTAACAGTAGTTCAAGCACCTGGACAAACCCGCCCACAGCGCCGAGGACTGGGACTTTAAACCCAAGATCCTATTTATAGTAACAAAATAAGATAAATTGc                                                                                                                                    >  2:203579/1‑298 (MQ=255)                                                                                                                                                                                    tGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACaaa                                                                                                         >  1:108386/1‑298 (MQ=255)                                                                                                                                                                                       agagATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAAtt                                                                                                      <  1:210747/298‑1 (MQ=255)                                                                                                                                                                                             aaGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGtt                                                                                                <  1:202637/298‑1 (MQ=255)                                                                                                                                                                                                                    gcgcCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCTTTTACAATGACCACATCCCAACAAt                                                                         >  2:209745/1‑298 (MQ=255)                                                                                                                                                                                                                           aGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGcc                                                                                                   <  1:357026/265‑1 (MQ=255)                                                                                                                                                                                                                           aGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGcc                                                                                                   >  2:357026/1‑265 (MQ=255)                                                                                                                                                                                                                            gTCGCTTCTTCACCAGAAAAGCCTTTGATTTTAACAGCAGTAGGGATAGGGCCCAGATCGACGGCCACATCGGGAGTCACTGGGGCGATATCATACAGAGGGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTc                                                                 <  2:320699/298‑1 (MQ=255)                                                                                                                                                                                                                                    ttCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATCACCACATCACACGAATGTGTAGTCACTCAAGt                                                         >  2:272949/1‑298 (MQ=255)                                                                                                                                                                                                                                         cAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGc                                                    >  1:256602/1‑298 (MQ=255)                                                                                                                                                                                                                                          aGAATATCCTTTGTTGTTCACAGCAGTAGGGATATGGCTGAGATCGACAGCCACATCGGGATGAACTGAGGCGATATCATACTGGGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTGAACGGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGCATGTGTAGTCACGCAAGTTTAGCg                                                   <  2:413118/298‑1 (MQ=255)                                                                                                                                                                                                                                                         tttCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAAtt                                    <  1:209745/298‑1 (MQ=255)                                                                                                                                                                                                                                                         tttCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAAtt                                    <  1:190721/298‑1 (MQ=255)                                                                                                                                                                                                                                                             acaGCAGTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAATTGCCg                                >  1:343491/1‑298 (MQ=255)                                                                                                                                                                                                                                                                gcagTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAATTGCCGTaa                             <  1:272949/298‑1 (MQ=255)                                                                                                                                                                                                                                                                gcagTAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAATTGCCGTaa                             >  1:152586/1‑298 (MQ=255)                                                                                                                                                                                                                                                                    tAGGGATATGGCTCAGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAATTGCCGTAATCAg                         >  1:130450/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                  aGATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCCCCTCACAAGAAAGTATAGTCACGCAAGTTTAACGGTTATTCATTTAATTCCCGTAATCAGGAACCCAAATTAcc           >  2:129056/1‑297 (MQ=255)                                                                                                                                                                                                                                                                                   gATCGACAGCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAATTGCCGTAATCAGGAACCTAACTTACGt          <  1:158174/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                           gCCACATCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAATTGCCGTAATCAGGAACCTAACTTACGTAATTAACa  <  1:126067/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                    ACACCGGCTTTTTTCAGCACTTCAGCAGCAATCGCAACTGTGGTGTTAACCGGGTTAGTGATAATACCAATGCACGCTTTCGGGCAGGTTTTCGAAACTTGCTGTACCAGGTTTTTCACGATGCCGGCGTTAACGTTAAACAGGTCGGAACGATCCATACCCGGTTTACGCGCTACACCTGCAGAGATAAGAACGACATCTGCGCCTTCCAGCGCCGGAGTCGCATCTTCACCAGAAAAACCTTTGATTTTCACAGCAGTAGGGATATGGCTCAGATCGACAGCCACACCGGGAGTCACTGGAGCGATATCATACAGAGAGAGTTCTGAACCTGAAGGCAGTTGGGTTTTTAACAGTAGTGCAAGCGCCTGGCCAATACCGCCAGCAGCGCCGAGGACTGCGACTTTCATCCTAAACTCCTTATTATATTGATAAACTAAGATATGTTGCTCCGCTGCCGCGACCTTAATCCACAAAATTGCCGTTTACAATGACCACATCTCAAGAATGTGTAGTCACGCAAGTTTAGCGTTTATGCATTTAATTGCCGTAATCAGGAACCTAACTTACGTAATTAACA  >  CP006584/3505655‑3506234

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑