Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	3,545,695	G→A	noncoding (2805/2931 nt)	LY180_16870 ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	3,545,695	0	G	A	100.0%	24.6 / NA	18	noncoding (2805/2931 nt)	LY180_16870	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/9);  total (9/9)

GGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATTTAGCTACC  >  CP006584/3545404‑3545773                                                                                                                                                                                                                                                                                                    |                                                                               gggtacgccACACTATCGTCGGGGGTTAGGCGTTTCGCGTCTGGGGTCGGCATGGGGTCAGTGGGGACCACCGCGCTAAGGCCGCCAGGCAAATTATGTTTTATCAGACCGCTTCTGCGTGCTGATTTAATCTGTATCAGGCTGAAACGCTTCTCTAATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCa                                                                          <  2:325920/291‑1 (MQ=11) ggAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCa                                                                          <  1:137250/298‑1 (MQ=14)     aCCCCACCCTCACATCGGCGCTACTGCGTTTCTCGTCGGAGTTCGGCATGGGGCCGGGGGGGCCCACCGCGCTAAGGCCGCCAGGCAAATTGTGTTTGATCAGACCGCTTCTGCGTTCTTATTTAATCTGTAGCAGGCGGAAAATCTTCTCTCATCCGCCAAACCACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGga                                                                      <  2:152058/298‑1 (MQ=11)         cgcactgactgcGGTGCTACGTCGTTGCCCTCCTTGGTTCGGACTGGGGTAAGGTGGGCCACCCGCGCTCAGGCCGCCAGGCAAATTCTGTTTACTCTGACCGCGTCTGCGGTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTGCATGAGTACCGGTTGGCTCAACGCATCGCTGCGATTACACACCCGGCCTAGCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAAc                                                                  <  2:70892/287‑1 (MQ=11)         cacaCTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAAc                                                                  <  1:62049/298‑1 (MQ=14)                ccATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCAtctc                                                           >  1:8086/1‑298 (MQ=14)                      gcgcTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCa                                                     >  1:406973/1‑298 (MQ=14)                          tACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGtt                                                 <  1:410733/298‑1 (MQ=14)                             ggCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACCTTCCTTCCGGACCCTTAAAGGGCCAGGGAAACCTCATCCCGGGGCAAAttttg                                              >  2:240117/1‑296 (MQ=11)                                gTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACAGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGGCGTCGTCTTCAACGTTCCTTCAGGCCCCTTAAAGGTTCAGGGCGAACTCATCTCGGGGCCAGTTTCGTGc                                           >  2:39309/1‑298 (MQ=11)                                 tttCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCt                                          <  1:182508/298‑1 (MQ=14)                                  ttCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCCTCCGCCCAAACACCTTCGGCGTTGTAAGCTTAAGCCTCACGGTTCATTAGTACCGGTTAGCCCAACCCATCGCCGCCCTTACACACCCGGCCCACTACCGCCGCCGTCTGCAAACGTCCTTCCCGCCCCTCAAAGGGTCCGGGAGACCTCGTCACCGGGCCAGCTTctgtctt                                         >  2:217343/1‑292 (MQ=11)                                     aCTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCGCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGCCGTCGTCTCCAACGCTCCTTCAGGACCCTTAAAGTGCCAGGGAGAACTCATATCGGGGCAAGTCTCTGGCTTAgt                                      >  2:411886/1‑297 (MQ=11)                                                  gCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACt                         >  1:194909/1‑298 (MQ=14)                                                        ggTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTAtctc                   <  2:406973/298‑1 (MQ=14)                                                               tGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATTTCTTCCGCa            >  1:314763/1‑298 (MQ=9)                                                                gggACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATGTAATCTGTGTCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTAAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCAt           >  1:268895/1‑298 (MQ=9)                                                                         gcgcTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTTAACGCATCGCTGCGGTTACACATCCGTCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATTTAGCTAcc  <  1:168800/298‑1 (MQ=9)                                                                                                                                                                                                                                                                                                    |                                                                               GGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATTTAGCTACC  >  CP006584/3545404‑3545773

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑