Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	4,023,563	T→C	L4P (CTC→CCC)	rnpA →	ribonuclease P

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	4,023,563	0	T	C	98.2%	188.6 / NA	55	L4P (CTC→CCC)	rnpA	ribonuclease P
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (25/29);  total (26/29)
Fisher's exact test for biased strand distribution p-value = 4.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTG  >  CP006584/4023276‑4023850                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                gtgtACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCCCTTTTCAATCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAACGGTCGTCAGGTTCTGGCACGTCTGCGACGTAAAGTCCATGCTCGTGGTACGGTTTCACCATTCTAACCCTAACCCCCGATTGGGTAAGCTAGCAtccccc                                                                                                                                                                                                                                                                                       >  2:110671/1‑293 (MQ=255)         ttattaCAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGtt                                                                                                                                                                                                                                                                               >  1:236972/1‑298 (MQ=255)                  tCCGGCCTCTTTAATCCCCCGTGTTTTCGGTGTCAATCGTTTCATTTTTCGGCGGATAGCCAATAAAGCCATTGAATTTATTCAAGTTTATGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGGGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGtt                                                                                                                                                                                                                                                                      <  2:267359/298‑1 (MQ=255)                         tctTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAACAAAGCTAACCCCTGAGGGGTTAAGCCCCCATTTCACAGGGAGTTACGCTTGttcaacccc                                                                                                                                                                                                                                                               >  2:360713/1‑291 (MQ=255)                                            tCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAGGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCt                                                                                                                                                                                                                                            <  1:196392/298‑1 (MQ=255)                                                tgtCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCca                                                                                                                                                                                                                                        >  1:250750/1‑298 (MQ=255)                                                                                 tAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAACGGCAGCGCTAGTCTGACCGTTTCTCAGTACTAAAGCTAACCCCTGAGTGGTTAAGCCCGCCTTTCCCAGGGAGTTACCCTTGTTAAGTCCCAGTCAATCCACATTCGCTTTCCAGCAGACACAACGGGCTGCCACGCCCCaacctaa                                                                                                                                                                                                       >  2:117998/1‑293 (MQ=255)                                                                                      ccATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATtc                                                                                                                                                                                                  <  1:258895/298‑1 (MQ=255)                                                                                                    aaaGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCAC‑ACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAAtt                                                                                                                                                                                   <  1:360713/297‑1 (MQ=255)                                                                                                      aGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACggg                                                                                                                                                                                                                          <  1:176755/258‑1 (MQ=255)                                                                                                      aGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACggg                                                                                                                                                                                                                          >  2:176755/1‑258 (MQ=255)                                                                                                      aGTTGAGGTACAGATGGCATTGAATCGCCTTTTTCCACCGTCTGTAATGAAGCGCAACCGTTTTCACGGCTTCCGTGCTCGTATGGCTACGAAAAATGGTCGGAAGGTTCTGGCACGTCGTCGTGCGAAAGGCCGCGCTCGTCTGACCGTGTCTAAGTAATAAAGCTAACACCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTc                                                                                                                                                                                  <  2:88744/298‑1 (MQ=255)                                                                                                           aGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCCCGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAACGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTAAATTCACATTCGTCTGCCAGCAGCCACAACGGCCGGCCACCACACAAATTACCATTTTCCgcccccgccatccgctgc                                                                                                                                                                             >  2:260607/1‑286 (MQ=255)                                                                                                           aGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTgg                                                                                                                                                                             <  1:391459/298‑1 (MQ=255)                                                                                                                       ccATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTACGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCCAGCAATTCCCTTTCGTCTTCCACCAGCCACAACGGCCCGGCACCCCGCAAATTCCCATTCCCGGCCCCCTGAATTCGCTGGGGCCTCCCCGTa                                                                                                                                                                 >  2:63993/1‑298 (MQ=255)                                                                                                                       ccATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGCCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTa                                                                                                                                                                 >  1:305422/1‑298 (MQ=255)                                                                                                                                              tCTGTACTGAAGCGCAACCGTTCTCACGGCTCCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACTCCGCAAAGTACCATTCTCCGCCGCCTGAATTCGCTGGGGCATCCCCGGCACCGTCTTAAAGTCGCCCAGaaa                                                                                                                                          >  2:335474/1‑298 (MQ=255)                                                                                                                                                      gAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTCCGCCTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCCCGGCCGCCTGAATTCCCTGGGGCCTCCCCGTATCCGCCTTCCAGTCGCACAGGAAAACGTTCg                                                                                                                                  >  2:385027/1‑298 (MQ=255)                                                                                                                                                      gAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCg                                                                                                                                  <  1:58364/298‑1 (MQ=255)                                                                                                                                                         gcgcAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGAcg                                                                                                                               <  1:1843/298‑1 (MQ=255)                                                                                                                                                            cAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACCTTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCCCTGGGGCATCCCCGGCACGGTCTTCCACTCGCCAAGAAAAACGTTCGACgcgc                                                                                                                            >  2:149091/1‑298 (MQ=255)                                                                                                                                                             aaCCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGTAAAACGTTCGACGCGcc                                                                                                                           >  1:221798/1‑298 (MQ=255)                                                                                                                                                               ccGTTCTCACGGCTTCCGTGCTCGTCTGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAACGGCCGCGCTCGTCTGACCGTTTCTACGCAATAAAGCTAACACCTGCGTGGTTAAGCCCGCATTACCCCGGGACTTACGCTGTGTAACTCCAAATCAATTCACATTTGTATTCAAGCAGCCACAACGGGACGGCACGCCGCAAATTACCATTCTCGCCCGCCGGAATCGCCCGGGTCATCCACACAGCGGTCTGAAAGTCGCCAACAAAAACGtctcagccaccca                                                                                                                         >  2:336661/1‑286 (MQ=255)                                                                                                                                                                cGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCAt                                                                                                                        <  1:152827/298‑1 (MQ=255)                                                                                                                                                                  ttCTCACTGCTTGCGTGCTCGGATGTACACTAACAAGGGGCGGCAGGTTATTGGAAGTCGTGGGGATAAAGGCCCGGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGAATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATGCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATGCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGa                                                                                                                      <  2:236972/298‑1 (MQ=255)                                                                                                                                                                     tcACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACg                                                                                                                   >  1:331982/1‑298 (MQ=255)                                                                                                                                                                       aCGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCa                                                                                                                 >  2:343325/1‑298 (MQ=255)                                                                                                                                                                                             tactaAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGAcgcg                                                                                           >  1:419088/1‑298 (MQ=255)                                                                                                                                                                                               ctaAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTg                                                                                         <  1:308805/298‑1 (MQ=255)                                                                                                                                                                                                 aaaaaTGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATCACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGaa                                                                                       >  1:268350/1‑298 (MQ=255)                                                                                                                                                                                                   aaaTGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAg                                                                                     <  1:343325/298‑1 (MQ=255)                                                                                                                                                                                                   aaaTGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAg                                                                                     <  1:63993/298‑1 (MQ=255)                                                                                                                                                                                                     aTGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCt                                                                                   <  1:335474/298‑1 (MQ=255)                                                                                                                                                                                                      tGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACCTTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCtt                                                                                  <  2:76263/298‑1 (MQ=255)                                                                                                                                                                                                         tcgtcAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCg                                                                               <  1:248045/298‑1 (MQ=255)                                                                                                                                                                                                           gtcAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTc                                                                             >  1:114947/1‑298 (MQ=255)                                                                                                                                                                                                                     ggCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACa                                                                   <  1:260607/298‑1 (MQ=255)                                                                                                                                                                                                                           tcgtcgtGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACt                                                             >  1:174537/1‑298 (MQ=255)                                                                                                                                                                                                                           tcgtcgtGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATACGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTATGACGCGGGAAAGCTTCCGTCTCGCCCAACATGAAcc                                                             >  2:271178/1‑297 (MQ=255)                                                                                                                                                                                                                             gtcgtGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCCGTCTTCCCGTCGCCAAGAAAAAAGTTCGACGCCCCCAAGAACGCAATCGGCATAACCGACAGACCCCTGAAAGGTTCCGTCTTCGCCCACTACAACTcc                                                           >  2:426174/1‑298 (MQ=255)                                                                                                                                                                                                                                               cTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCgtgg                                         <  1:336661/298‑1 (MQ=255)                                                                                                                                                                                                                                                tCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCgtggt                                        <  1:325885/298‑1 (MQ=255)                                                                                                                                                                                                                                                 ggTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCgtggtg                                       <  2:331982/297‑1 (MQ=255)                                                                                                                                                                                                                                                                  gTAATAAAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGt                      <  1:17955/298‑1 (MQ=255)                                                                                                                                                                                                                                                                  gTAATAAAGCTAACCCCTGAGTGGCTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGCTTAAACGTCTGACGCCGGAAAGCTTCCGGCTGCCCAAACATGAACTCCAGGCCATGGATTTCTGGGTGGTGGCGAAAAAAGGGGt                      >  2:183998/1‑298 (MQ=255)                                                                                                                                                                                                                                                                     ataaAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGc                   <  1:117998/298‑1 (MQ=255)                                                                                                                                                                                                                                                                     ataaAGCTAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGc                   <  1:385027/298‑1 (MQ=255)                                                                                                                                                                                                                                                                            tAACCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTc            >  1:257472/1‑298 (MQ=255)                                                                                                                                                                                                                                                                              aCCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTCCAGTCGCCAAGAAAACCGTTCGACGCGCCCATGAACGCACTCGGATTAAACGTCTGACGCGTGAAAGCTTCCCTCTGCGCCAACATGACACCCCGCCCATGGATTTACCGGGGGGTGCCAAAACAGGGGtgtcccactccgc          >  2:366458/1‑286 (MQ=255)                                                                                                                                                                                                                                                                              aCCCCTGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAACAGTCTGACGCGTGAAAGCTTCCGTCTGCGCCCACATGAACTCCCGGCCATGGATTTCGTGGTGGTGGCGAAACAAGGGGTTGcggcccccgc          >  2:238925/1‑289 (MQ=255)                                                                                                                                                                                                                                                                               ccccTGTGTTGTTCAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGAt         <  2:250750/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                  cTGAGTGGCTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAAc      <  1:183998/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                   tGAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAAcc     <  1:110671/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                    gAGTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATACGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCg    <  1:271178/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                      gTGGTTAAGCCCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTg  <  1:375625/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                                                                GTGTACAATTATTACAATCCGGCCTCTTTAATCACCCATGGCTTCGGTGTCCATCGTTTCATTTTTCGGCGGATATCCAATAAAGCCATTGAATTTATTCAAGTTTAGGTAGAAATCGCCATGAAACGCACTTTTCAACCGTCTGTACTGAAGCGCAACCGTTCTCACGGCTTCCGTGCTCGTATGGCTACTAAAAATGGTCGTCAGGTTCTGGCACGTCGTCGTGCTAAAGGCCGCGCTCGTCTGACCGTTTCTAAGTAATAAAGCTAACCCCTGAGTGGTTAAGCTCGCATTTCCCAGGGAGTTACGCTTGTTAACTCCCAGTCAATTCACATTCGTCTTCCAGCAGCCACAACGGGCTGGCACGCCGCAAATTACCATTCTCGGCCGCCTGAATTCGCTGGGGCATCCCCGTATCGGTCTTACAGTCGCCAAGAAAAACGTTCGACGCGCCCATGAACGCAATCGGATTAAACGTCTGACGCGTGAAAGCTTCCGTCTGCGCCAACATGAACTCCCGGCTATGGATTTCGTGGTGGTGGCGAAAAAAGGGGTTGCCGACCTCGATAACCGTG  >  CP006584/4023276‑4023850

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑