Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	4,305,124	G→A	G42G (GGG→GGA)	LY180_20645 →	rhsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	4,305,124	0	G	A	92.3%	65.0 / NA	27	G42G (GGG→GGA)	LY180_20645	rhsA
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base A (9/15);  total (12/15)
Fisher's exact test for biased strand distribution p-value = 1.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGTAATTTGCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGCCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACA  >  CP006584/4304832‑4305312                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                             gcaAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGTAATTTGCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGAc                                                                                                                                                                                         >  1:320702/1‑298 (MQ=255)   aaaTAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGTAATTTGCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCCAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGt                                                                                                                                                                                       >  1:134398/1‑298 (MQ=255)          aCCGGGTTTCCGAAAAAAGGGGCGCACGGCGCTTTTTTTTGGCCTTTAATTTGCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGGTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCa                                                                                                                                                                                <  2:397726/298‑1 (MQ=255)            cGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGTAATTTGCCTTTTATAAATTCTGCAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGGACGTATTGGTGCCCCCACCGGCGTGGCCTGTTGGGTGTGCCTCGGCGGGGTGACCTCCCGCCATc                                                                                                                                                                              >  2:307367/1‑298 (MQ=255)              gATTTCCGAAAAAATCGCCGCACGGCGCTTTTTTTGTGCCTGTAATTTGCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGGGCGGAAAACCGGCGGCGCGTCAGGGCGCCATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCTGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCg                                                                                                                                                                            <  1:428944/298‑1 (MQ=255)                               ccgcACGGCGCTTTTTTTGTGCCTGTAATTTGCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCgg                                                                                                                                                           <  1:307367/298‑1 (MQ=255)                                       cgcTTTTTTTGTGCCTGTAATTTGCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGCGCGGAAAACCGGCGGCGCGTCCGGGCGACATGACGCAGTATGGCGGTAGCAGTGTCCCGGTTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGGGGCCTGTTCGGTGTGCCCCGGCGTGGTGACTTCCGGCCCCCCGGCCCATCCCCTGCCCGTGGCaacgt                                                                                                                                                   >  2:428078/1‑295 (MQ=255)                                             ttttGTGCCTGTAATTTGCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTc                                                                                                                                             <  1:422931/298‑1 (MQ=255)                                                                 ttttATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGcc                                                                                                                         >  1:39351/1‑298 (MQ=255)                                                                 tttcttaAATTATGAAAATGTCCGGCGTTTTACTTCTGTTTTGTTTCCGGTTGGTGATTGTGGCTTGGCTTCTGGTTCTTATTTGTTTAAAAAGGATTTTACGATGAGCGGAACACCGGCGGCGCGTCAGGGCGCCATGACGACGTATGGCGGTAGCATTGTCCCGGGTTCAGCCGGGGTACGTATGGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGcc                                                                                                                         <  2:191140/293‑1 (MQ=14)                                                                         aTTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATc                                                                                                                                                                     <  2:185350/246‑1 (MQ=255)                                                                         aTTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATc                                                                                                                                                                     >  1:185350/1‑246 (MQ=255)                                                                         aTTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTgcccgg                                                                                                                 >  1:168970/1‑297 (MQ=255)                                                                         aTTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGCTGTTGACTGGCGCTAGGCCTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGCAAAACCGGCGCCGCGTCAGGGCGACATGACCCCGTCTGGCGGTAGCATTGTCCAGGGATCACCCGGGGCCCCTCCTGGTCCCCCCACCGGCGCGGCCTCCTCGCTGTCCCCCGGCGGCGCGCACTCCGGCCCTCCGTCCCACCCCCCGCCCGGGCCAATCGGCCTCCCCGGTGCAACCCACCTCGCCCTgccctg                                                                                                                 >  2:424571/1‑296 (MQ=255)                                                                            cTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGcccggccc                                                                                                              <  1:156258/298‑1 (MQ=255)                                                                                                 aCTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCg                                                                                         <  1:82780/298‑1 (MQ=255)                                                                                                  cTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTTTCCAGGGTTCAGCCGGGGTACGTATTGGGGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCCTCCCGTCAACCCCCTGCCCCGTGCAAAGGTCCTTCCCGGTGAAACCGACACCCCCCTGCCCGGCCCCCCGCCGTTCCCCCTCTccccc                                                                                        >  2:376683/1‑296 (MQ=18)                                                                                                          cATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTAcag                                                                                <  1:184586/298‑1 (MQ=255)                                                                                                                     gttgACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGa                                                                     <  1:428078/298‑1 (MQ=255)                                                                                                                              gtgtatgCTTTTGGTGCTTAGTGATTTCAAGAGGTTTTTACGAGGGGGGGAAAACCGGCGGCGCGTCAGGGCGACAGGACGCAGTATGGCGGTAGCAGTGTCCAGGGTTCAGCCGGGGTACGGATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAgcgc                                                            <  2:131292/292‑1 (MQ=11)                                                                                                                                         tGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTg                                                 >  1:365065/1‑298 (MQ=255)                                                                                                                                             tCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGa                                             >  1:294169/1‑298 (MQ=255)                                                                                                                                               ttatttatTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAACATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGCTCGGTGTGCCCCGGCGGAGTGACGCCCGGCCCTCCGGTCAATCCCCTGCGCGGTGCAACGGCCCTTCCCGGGGAAACCGGCACCCCCCTGCCCTGCCCGCCGCCGTTCACCCCCCCCCGCACCTACAGCAGTTCCCGGCACAACCCGCCCGCGCCGGTGGGgcgc                                           >  2:158706/1‑298 (MQ=14)                                                                                                                                                   ttatttCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCg                                       <  1:216917/298‑1 (MQ=255)                                                                                                                                                        tCAAAAGGATTTTACGATGAGCGGAAACCCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGcccc                                  <  1:188658/298‑1 (MQ=255)                                                                                                                                                                         tGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCTCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGc                 <  1:423809/298‑1 (MQ=14)                                                                                                                                                                                        cggcgCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACa  <  1:25328/298‑1 (MQ=11)                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                             GCAAATAAGACCGGATTTCCGAAAAAAGCGCCGCACGGCGCTTTTTTTGTGCCTGTAATTTGCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGCCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGCTTACA  >  CP006584/4304832‑4305312

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑