Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	1,645,301	T→G	intergenic (+1095/+165)	LY180_07765 → / ← LY180_07770	transposase/sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	1,645,301	0	T	G	81.2%	33.9 / ‑4.0	16	intergenic (+1095/+165)	LY180_07765/LY180_07770	transposase/sulfatase
Reads supporting (aligned to +/- strand):  ref base T (3/0);  new base G (9/4);  total (12/4)
Fisher's exact test for biased strand distribution p-value = 5.29e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.72e-01

CAGCGAGTCGCTCTTTGTGGAGGTGACGATTACCTAACAATCGGTCGATTCGTTTGATGTTATGTTTTGTTCTCGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTAAGAGTGAGAGTTTTACAGTCAAGTAATGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATCTTGTTGTCTGATTATTGATTTTTCGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAAC  >  CP006584/1645168‑1645587                                                                                                                                      |                                                                                                                                                                                                                                                                                               cAGCGAGTCGCTCTTTGTGGAGGTGACGATTACCTAACAATCGGTCGATTCGTTTGATGTTATGTTTTGTTCTCGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTAAGAGTGAGAGTTTTACAGTCAAGTAATGCGTTGCAAGCCCACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGTGCAGAAATGGTAAAGAGAGTAGAGTAAAATATCGAGTTAGCACATCTTGTTGTCTTATTATTTATTTTTCGATAAACCTGTTGATCAAATTACATGATTCGTATCTACattaac                                                                                                                            >  2:51573/1‑298 (MQ=11)   gCGAGTCGCTCTTTGTGGAGGTGACGATTACCTAACAATCGGTCGATTCGTTTGATGTTATGTTTTGTTCTCGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTAAGAGTGAGAGTTTTACAGTCAAGTAATGCGTGGCCAGCCAACGTTAAGCTGTTGAGTCGTGTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATTGAGTTCGCACCTCTTGTTTTCTGATTATTGATTTTTCGCGAAACCATTTGATCATATGACAAGATTTGTTTcccccatacctt                                                                                                                          >  2:546231/1‑287 (MQ=1)     gAGTCGCTCTTTGTGGCGGTGACGATTACCTCACAATCGGTCGACTCGTTTGATGTTATGTTTTGTTCTCGCTTTGTTTGGCAGGTTACGCCCAAGTTCTGTAAGAGTGTGAGTTTTACAGTCACGTAAGGCGTGGCAAGCCAACGTCAAGCTGTTGAGTCGCGTTAAGTGTAATTTGGGGTAGAATTGGTCAAGAGAGTCGTATCAACTATCGAGTTCGACCAGCTTGATGTCTGAGTATTGAATCTTCGCCAAGCCATTTGCTCATATGGCCAGATGTTTCTCTCCcttacattat                                                                                                                        >  2:634117/1‑292 (MQ=11)                                        aTCGGTCGATTCGTTTGATGTTATGTTTTGTTCTCGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTCAGAGCGCGAGTTTTACAGTCAAGTAATGCGTGGCAAGCCAACGCTAAGCTGTGGCGTCGTTTTAAGTGTAATTCGGGGCAGCATTAGTAAAGAGAGTCGTGTAAAATCTCGAGCTGGCACATCTTGTTGTTTGCTTATTAACTTTTCACGAAACAATTTAATCATATGACATATTGTTTACCATCCTTAACTAAAACATTATCATATACATCATCATGCGATTCATCAga                                                                                     >  2:544508/1‑297 (MQ=11)                                                    gTTTGATGTTATGTTTTGTTCTCGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTAAGAGTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAACTGGTAAAGAGAGTCGTGTCAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGTTTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTACCTTAATGATATTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATaaa                                                                         >  2:177662/1‑298 (MQ=255)                                                     tttGATGTTATGTTTTGTTCTCGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTAAGAGTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATaaaa                                                                        >  1:257445/1‑298 (MQ=25)                                                                 ttttGTTCTCGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTAAGAGTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAgt                                                            >  1:291107/1‑298 (MQ=255)                                                                         tcGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTAAGAGTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGACCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGc                                                    >  1:213618/1‑298 (MQ=255)                                                                              ttggtgggCAGGTTACGGCCAAGTTCGGTAAGAGTGGGAGTTTTACAGTTAAGTAAGGCGGTGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGAt                                               <  2:577307/298‑1 (MQ=255)                                                                                                     ttCGGTAAGAGTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCg                        >  1:427135/1‑298 (MQ=255)                                                                                                           tagagTGTGTGTTTTACAGTCAAATAAGGTGTGGCAAGCCAAGTTGAAGCTGTGGAGTTTGTGTAATTGTAATTGTGGGCAGAATTGGTAAAGGAAGTCGTTGAAAATATCGAGTTCGGACATTTTGTGGTCTGAGTATTGAGTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAGATCAGTAGGTGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAAc                  <  2:479090/297‑1 (MQ=18)                                                                                                            agagTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACt                 >  1:470574/1‑298 (MQ=255)                                                                                                             gagTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTg                >  1:241145/1‑298 (MQ=255)                                                                                                              agTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGt                                                                 >  1:424962/1‑248 (MQ=255)                                                                                                              agTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGt                                                                 <  2:424962/248‑1 (MQ=255)                                                                                                                           cAGGCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAAc  <  2:294781/298‑1 (MQ=255)                                                                                                                                      |                                                                                                                                                                                                                                                                                               CAGCGAGTCGCTCTTTGTGGAGGTGACGATTACCTAACAATCGGTCGATTCGTTTGATGTTATGTTTTGTTCTCGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTAAGAGTGAGAGTTTTACAGTCAAGTAATGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATCTTGTTGTCTGATTATTGATTTTTCGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAAC  >  CP006584/1645168‑1645587

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑