Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	1,645,397	C→T	intergenic (+1191/+69)	LY180_07765 → / ← LY180_07770	transposase/sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	1,645,397	0	C	T	86.7%	76.8 / ‑4.8	30	intergenic (+1191/+69)	LY180_07765/LY180_07770	transposase/sulfatase
Reads supporting (aligned to +/- strand):  ref base C (4/0);  new base T (15/11);  total (19/11)
Fisher's exact test for biased strand distribution p-value = 2.68e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGAGTCGCTCTTTGTGGAGGTGACGATTACCTAACAATCGGTCGATTCGTTTGATGTTATGTTTTGTTCTCGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTAAGAGTGAGAGTTTTACAGTCAAGTAATGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATCTTGTTGTCTGATTATTGATTTTTCGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAACCTTTTGCGGGGACACGTTCACGATTTTTGAACAGTGAAGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGG  >  CP006584/1645168‑1645685                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                 cAGCGAGTCGCTCTTTGTGGAGGTGACGATTACCTAACAATCGGTCGATTCGTTTGATGTTATGTTTTGTTCTCGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTAAGAGTGAGAGTTTTACAGTCAAGTAATGCGTTGCAAGCCCACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGTGCAGAAATGGTAAAGAGAGTAGAGTAAAATATCGAGTTAGCACATCTTGTTGTCTTATTATTTATTTTTCGATAAACCTGTTGATCAAATTACATGATTCGTATCTACattaac                                                                                                                                                                                                                              >  2:51573/1‑298 (MQ=11)   gCGAGTCGCTCTTTGTGGAGGTGACGATTACCTAACAATCGGTCGATTCGTTTGATGTTATGTTTTGTTCTCGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTAAGAGTGAGAGTTTTACAGTCAAGTAATGCGTGGCCAGCCAACGTTAAGCTGTTGAGTCGTGTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATTGAGTTCGCACCTCTTGTTTTCTGATTATTGATTTTTCGCGAAACCATTTGATCATATGACAAGATTTGTTTcccccatacctt                                                                                                                                                                                                                            >  2:546231/1‑287 (MQ=1)     gAGTCGCTCTTTGTGGCGGTGACGATTACCTCACAATCGGTCGACTCGTTTGATGTTATGTTTTGTTCTCGCTTTGTTTGGCAGGTTACGCCCAAGTTCTGTAAGAGTGTGAGTTTTACAGTCACGTAAGGCGTGGCAAGCCAACGTCAAGCTGTTGAGTCGCGTTAAGTGTAATTTGGGGTAGAATTGGTCAAGAGAGTCGTATCAACTATCGAGTTCGACCAGCTTGATGTCTGAGTATTGAATCTTCGCCAAGCCATTTGCTCATATGGCCAGATGTTTCTCTCCcttacattat                                                                                                                                                                                                                          >  2:634117/1‑292 (MQ=11)                                        aTCGGTCGATTCGTTTGATGTTATGTTTTGTTCTCGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTCAGAGCGCGAGTTTTACAGTCAAGTAATGCGTGGCAAGCCAACGCTAAGCTGTGGCGTCGTTTTAAGTGTAATTCGGGGCAGCATTAGTAAAGAGAGTCGTGTAAAATCTCGAGCTGGCACATCTTGTTGTTTGCTTATTAACTTTTCACGAAACAATTTAATCATATGACATATTGTTTACCATCCTTAACTAAAACATTATCATATACATCATCATGCGATTCATCAga                                                                                                                                                                                       >  2:544508/1‑297 (MQ=11)                                                    gTTTGATGTTATGTTTTGTTCTCGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTAAGAGTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAACTGGTAAAGAGAGTCGTGTCAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGTTTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTACCTTAATGATATTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATaaa                                                                                                                                                                           >  2:177662/1‑298 (MQ=255)                                                     tttGATGTTATGTTTTGTTCTCGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTAAGAGTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATaaaa                                                                                                                                                                          >  1:257445/1‑298 (MQ=25)                                                                 ttttGTTCTCGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTAAGAGTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAgt                                                                                                                                                              >  1:291107/1‑298 (MQ=255)                                                                         tcGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTAAGAGTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGACCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGc                                                                                                                                                      >  1:213618/1‑298 (MQ=255)                                                                              ttggtgggCAGGTTACGGCCAAGTTCGGTAAGAGTGGGAGTTTTACAGTTAAGTAAGGCGGTGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGAt                                                                                                                                                 <  2:577307/298‑1 (MQ=255)                                                                                                     ttCGGTAAGAGTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCg                                                                                                                          >  1:427135/1‑298 (MQ=255)                                                                                                           tagagTGTGTGTTTTACAGTCAAATAAGGTGTGGCAAGCCAAGTTGAAGCTGTGGAGTTTGTGTAATTGTAATTGTGGGCAGAATTGGTAAAGGAAGTCGTTGAAAATATCGAGTTCGGACATTTTGTGGTCTGAGTATTGAGTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAGATCAGTAGGTGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAAc                                                                                                                    <  2:479090/297‑1 (MQ=18)                                                                                                            agagTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACt                                                                                                                   >  1:470574/1‑298 (MQ=255)                                                                                                             gagTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTg                                                                                                                  >  1:241145/1‑298 (MQ=255)                                                                                                              agTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGt                                                                                                                                                                   <  2:424962/248‑1 (MQ=255)                                                                                                              agTGAGAGTTTTACAGTCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGt                                                                                                                                                                   >  1:424962/1‑248 (MQ=255)                                                                                                                           cAGGCAAGTAAGGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAAc                                                                                                    <  2:294781/298‑1 (MQ=255)                                                                                                                                                   ttagtgtttctcttGTGTCGTGTTAATTGTAATTCGGGGCAGGATTGGTAATGAGAGTTGTGTAGAATATCGAGTTCTAACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGTTCATATGACAAGATGTGTATCTACCTTAACTTAATGATTGTGATAAAAATCATTAGTGGATTCATCAGTGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAACCTTTTGCGGGGACACGTTCACGAt                                                                            <  2:89499/286‑1 (MQ=21)                                                                                                                                                   aaCGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCACCAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAACCTTTTGCGGGGACACGTTCACGAt                                                                            >  2:327301/1‑298 (MQ=255)                                                                                                                                                            cTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAACCTTTTGCGGGGACACGTTCACGATTTTTGAACa                                                                   <  1:189359/298‑1 (MQ=255)                                                                                                                                                             tGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAACCTTTTGCGGGGACACGTTCACGAttagatcggaa                                                                  >  1:89499/1‑289 (MQ=255)                                                                                                                                                                      gTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAACCTTTTGCGGGGACACGTTCACGATTTTTGAACAGTGAAGGGGa                                                         >  1:376326/1‑298 (MQ=255)                                                                                                                                                                      gTTTTAAGTGTAAGTTGGGGAAGAATTGGTAAAGATGTTAGTGTAAAATATCGAGTTGGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAGAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGGGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAACCTTTTGCGGGGACACGTTCACGATTTTTGAACAGTGAAGGGGa                                                         <  2:278847/298‑1 (MQ=25)                                                                                                                                                                        tttAAGGGTAATTCGGGAATGAATTTGTAAAGGGAGTCGTGTAAAATTTCGAGTTCGTACTTTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAACCTTTTGCGGGGACACGTTCACGATTTTTGAACAGTGAAGGGGAGt                                                       <  2:3532/298‑1 (MQ=25)                                                                                                                                                                             gtgtAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGACCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAACCTTTTGCGGGGACACGTTCACGATTTTTGAACAGTGAAGGGGAGTTGTaa                                                  <  2:213618/298‑1 (MQ=255)                                                                                                                                                                                                            tCGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACACCGCCAATTGCCTCATCGGTTAATTGATCGCTGATATAACCTTTTTCGGGGACACTTTCAAGATTTTTGAACAGTGAAGGGGAGTGGTAATATTCCGTTCCTTCAGCGTGGAATACAATaa                   >  2:372834/1‑298 (MQ=37)                                                                                                                                                                                                             cGTGTAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAACCTTTTGCGGGGACACGTTCACGATTTTTGAACAGTGAAGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATaaa                  <  1:311941/298‑1 (MQ=255)                                                                                                                                                                                                               tgtAAAATATCGAGTTCGGAATATTTGTTGTCTGATTATTGATTTTTGGCAAAGCCATTTGATCATATGAAAAGATGTTTATCTAACTTAACTTAATGATTTTGAAAAAAATAATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAACCTTTTGCGGGGACACGTTCACGATTTTTGAACAGTGAAGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGt                <  2:257445/298‑1 (MQ=255)                                                                                                                                                                                                                 tAAAATATCGAGTTCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAACCTTTTGCGGGGACACGTTCACGATTTTTGAACAGTGAAGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTaa              >  1:13426/1‑298 (MQ=255)                                                                                                                                                                                                                          gAGTTCGCACATTTAGTTGTCTGATTATTGATTTTTGGCGAAACCATTCGATCATATGACCAGATGTGTATATACCTTAACTTAATGATTTTGATAACTATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGTCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATCTAACCTTTTGCGGGGCCCAGTTCACGATTTTTGAACAGTGAAGGGGAGTTGTAATAACCAGTCCGTACAGAGTAGAATCCCCTCAAGTacaaaccgcca     >  2:55951/1‑288 (MQ=25)                                                                                                                                                                                                                             ttCGCACATTTTGTTGTCTGATTATTGATTTTTGGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCCGATATAACCTTTTGCGGGGACACGTTCACGATTTTTGAACAGTGAAGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACgg  >  1:197987/1‑298 (MQ=255)                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                                                                 CAGCGAGTCGCTCTTTGTGGAGGTGACGATTACCTAACAATCGGTCGATTCGTTTGATGTTATGTTTTGTTCTCGCTTTGGTTGGCAGGTTACGGCCAAGTTCGGTAAGAGTGAGAGTTTTACAGTCAAGTAATGCGTGGCAAGCCAACGTTAAGCTGTTGAGTCGTTTTAAGTGTAATTCGGGGCAGAATTGGTAAAGAGAGTCGTGTAAAATATCGAGTTCGCACATCTTGTTGTCTGATTATTGATTTTTCGCGAAACCATTTGATCATATGACAAGATGTGTATCTACCTTAACTTAATGATTTTGATAAAAATCATTAGGGGATTCATCAGGGTAAAGCATAAAAGGCTGGTCAAGTGTTTTGGCACGATCAACAACGCCAATTGCCTCATCGGTTAACTGATCGCTGATATAACCTTTTGCGGGGACACGTTCACGATTTTTGAACAGTGAAGGGGAGTTGTAATATGCCGTTCCTGCAGCGTGGAATCCCATAAAGTAATCAAAGCCACGG  >  CP006584/1645168‑1645685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑