Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	2,251,586	T→C	E104E (GAA→GAG)	LY180_10745 ←	major tail tube protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	2,251,586	0	T	C	92.9%	41.3 / ‑1.8	29	E104E (GAA→GAG)	LY180_10745	major tail tube protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (11/15);  total (12/17)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.04e-01

TTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCAATCATGTTGATGGTGTCGATTTCGACCAGCTCCTTACCGTCCATCGTCAGCCGGAAATAGGTGCAGACCACGGAGATTTTCGACTCGGTGTCTTCTCCCTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACTTCGACGGCCACCGTTTCGCCGGTATCGTCACGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATCCCCGCACCGCGATAGTTTTCGAGCTTACGGGTCAGTTTTGGCAGCGTGACGGACTTTGCAACGCCCTGATAGCTGTAGCCGTTCAGAAAGACGTTCATTAACT  >  CP006584/2251291‑2251875                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                  gtggccAGGGTAACACCATTTGCCTTGGTAAGGGTTTCCTCCCTTCGTCAGCGGGGTGAACCGGCCAGCCAGGCTGACCGGATGAGACTGCCGGGCCGATATTGCGGGGTGGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCAATCATGGTGATGGTGTCGATTTCGACCAGCTCCTTACCGTCCATCGTCAGCCGGAAATAGGTGCAGACCACGGAGATTTTCGACTCGGTGTCTTCTCCCTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACTTc                                                                                                                                                                                                                                                                                                 <  2:76120/294‑1 (MQ=1)                                                                                                                        gcgagggcgaggtcGTTCTCCTTCTGAATCAGGGTGATGGTTTCGTGTTCGGACAGTTCCTTACCTGCCATCGGTGGCCGGAAATAGGTGTAGGCCACGGAGATTTTCGACTCGGTGTCTTCTCCCTGGTTGCCCTCGCCGGTGTCGTTTTCTTTGTGGCGTCCACGCAGGACCACCTCGGCGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGa                                                                                                                                                                          <  2:334471/285‑1 (MQ=11)                                                                                                                            ggTCGACGCCGTTCACCTTCTCAATCATGTTGATGGTGTCGATTTCGACCAGCTCCTTACCGTCCATCGTCAGCCGGAAATAGGTGCAGACCACGGAGATTTTCGACTCGGTGTCTTCTCCCTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGa                                                                                                                                                                      >  1:421388/1‑298 (MQ=14)                                                                                                                                       ttCACCTTCTCAATCATGTTGATGGTGTCGATTTCGACCAGCTCCTTACCGTCCATCGTCAGCCGGAAATAGGTGCAGACCACGGAGATTTTCGACTCGGTGTCTTCTCCCTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTg                                                                                                                                                           <  1:543836/298‑1 (MQ=14)                                                                                                                                                       tGTTGATGGTGTCGATTTCGACCAGCTCCTTACCGTCCATCGTCAGCCGGAAATAGGTGCAGACCACGGAGATTTTCGACTCGGTGTCTTCTCCCTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGtcatc                                                                                                                                           >  1:406808/1‑298 (MQ=14)                                                                                                                                                                   cGATTTCGACCAGCTCCTTACCGTCCATCGTCAGCCGGAAATAGGTGCAGACCACGGAGATTTTCGACTCGGTGTCTTCTCCCTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTc                                                                                                                               <  1:329868/298‑1 (MQ=14)                                                                                                                                                                    gATTTCGACCAGCTCCTTACCGTCCATCGTCAGCCGGAAATAGGTGCAGACCACGGAGATTTTCGACTCGGTGTCTTCTCCCTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCt                                                                                                                              >  1:380144/1‑298 (MQ=14)                                                                                                                                                                         cccccAGCCCCTTTCCGTCCCTCGTCCGCCGGAAATCGGTGCAGACCACGGAGATTTTCGACTCGGTGTCTTCTCCCGGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGCCGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCgg                                                                                                                         <  2:391041/295‑1 (MQ=11)                                                                                                                                                                          gcccAGCTCCTTCCCGTCCATCGTCAGCCGGAAATAGGTGCAGACCACGGAGATTTTCGACTCGGTGTCTTCTCCCTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCTGAGGTCTACCGGt                                                                                                                        <  1:588155/296‑1 (MQ=14)                                                                                                                                                                              aGCTCCTTACCGTCCATCGTCAGCCGGAAATAGGTGCAGACCACGGAGATTTTCGACTCGGTGTCTTCTCCCTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCAAGAGAACCGAATCCGGGAAGCCACCGCGCGACCACCCCATTGCCAGCCCACCAACACACAGACCGCGGTCTACCGGtcgcc                                                                                                                    >  2:462754/1‑294 (MQ=11)                                                                                                                                                                               gCTCCTTACCGTCCATCGTCAGCCGGAAATAGGTGCAGACCACGGAGATTTTCGACTCGGTGTCTTCTCCCTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACTTCGACGGCCACCGTTTCGCCGGCATCGTCCCGCTGGTAAGAGCCTGCAAAACGAATCCGCCCAGCATCCACACCGGGTCCGGCGGAAAGCCCCCAGCAACACGAATCCGGGAAGCCACCGAGCGACCAACCCATTGACAGCGCACCGCCATCCAGCCCACGGCCTACCGCTccgcg                                                                                                                   >  2:562264/1‑297 (MQ=11)                                                                                                                                                                                 tCCTTACCGTCCATCGTCAGCCGGAAATAGGTGCAGACCACGGAGATTTTCGACTCGGTGTCTTCTCCCTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGc                                                                                                                 <  1:32773/298‑1 (MQ=14)                                                                                                                                                                                        cGTCCATCGTCAGCCGGAAATAGGTGCAGACCACGGAGATTTTCGACTCGGTGTCTTCTCCCTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCCCCCAGATAACCGAACCCGGGAAGCCACCGAGCGACCCCTCCATTGACAGCGCCTCGTCATCCAGGCCGAGGGCTACCGGTCCGCTGCCGTTCac                                                                                                          >  2:478448/1‑297 (MQ=255)                                                                                                                                                                                          tcgaagctgtgCCGGAAAGATGTGATGACTATGGGGATTTTCGATTCGGTGTTTTTTCCCTGGTTGCCCTTGCCGGTGTCGATGTCCTGGTGACGTCCTCGCATGACCACCTCGTCGGCCACCTTTTGTGAGCTTTCGTCTCGCCGGTAAGGGCCTGCAAAAAGAAGCGGCAATGCAACCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAAGCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATcc                                                                                                        <  2:345461/288‑1 (MQ=11)                                                                                                                                                                                          cccgctgccggcgggAAATGGGTGCGGTCCACGGGGATTTTCGTTTCGGGTTTTTCTCCCTGTTTCCCCCTGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACGTGGACGGCAACCGTTTCGACGGTAGCGTCGCGCTGTGAAGAGCCTGCAAAACGAATCTGCACAGCAGCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATcc                                                                                                        <  2:421388/285‑1 (MQ=11)                                                                                                                                                                                                                          ggAGATTTTCGACTCGGTGTCTTCTCCCTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATCCCCGCACCGCGATAGTTTTCGAGCTTACGGGTc                                                                        <  1:386735/298‑1 (MQ=14)                                                                                                                                                                                                                            gggttcgcGACTTGGTTTGTTCTCCGTGTTTGCCCTCGCCGTTGTCATTTTCTTTCTGGCGTCCACGCATGACCACCTGGA‑GCGCACCGGTTCGCCGGTCTCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATCCCCGCACCGCGATAGTTTTCGAGCTTACGGGTCAGt                                                                     <  2:89026/291‑1 (MQ=11)                                                                                                                                                                                                                              tttttCGGCTCGGTGTCTCTCCCCTGTTTGGCCTCGCCGGGGTCGATTTCTGTCTGACGTCCGCGCATGACCACCTCGACGGCCACCGTGTCGCCGGTATCGTCGCGCTGGGAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATCCCCGCACCGCGATAGTTTTCGAGCTTACGGGTCAGtt                                                                    <  2:109115/297‑1 (MQ=11)                                                                                                                                                                                                                                   cGACTCGGTGTCTTCTCCCTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATCCCCGCACCGCGATAGTTTTCGAGCTTACGGGTCAGTTTTGGc                                                               <  1:632208/298‑1 (MQ=14)                                                                                                                                                                                                                                    gaccctgtgtgtACTCCCTGTTGGCGCTCGCGCGTGTCGATTGCTGTGTGACGTGCACGCCGGGCCGCCCAGAGGGACACCGGGTCGCCGGTAGCGTCGCGCGGGTAAGAGGCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGAGAACCGAAGCCGGGAAGCCACCGGGCGGCCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATCCCCGCACCGCGATAGTTTTCGAGCTTACGGGTCAGTTTTGGCa                                                              <  2:656801/292‑1 (MQ=11)                                                                                                                                                                                                                                      cTCGGTGTCTTCTCCCTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATCCCCGCACCGCGATAGTTTTCGAGCTTACGGGTCAGTTTTGGCAGc                                                            >  1:589356/1‑298 (MQ=14)                                                                                                                                                                                                                                                     cTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATCCCCGCACCGCGATAGTTTTCGAGCTTACGGGTCAGTTTTGGCAGCGTGACGGACTTTGCa                                             >  1:312210/1‑298 (MQ=14)                                                                                                                                                                                                                                                         ttGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATCCCCGCACCGCGATAGTTTTCGAGCTTACGGGTCAGTTTTGGCAGCGTGACGGACTTTGCAACGc                                         <  1:200530/298‑1 (MQ=14)                                                                                                                                                                                                                                                          ggaccTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATCCCCGCACCGCGATAGTTTTCGAGCTTACGGGTCAGTTTTGGCAGCGTGACGGACTTTGCAACGcc                                        <  1:462754/295‑1 (MQ=14)                                                                                                                                                                                                                                                             ccacGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCCGCAAAACGAATCGGCCCAGCATCCACACCGGTTGCGGCGTCAAACTCCCAGATAACCGAATCCGGGAAGCCGCCGAGCGACCAGTCCATTGCCGGCGCAGCGTCACCAAGGCCGAGGTCTACCGGTCCGCTCCCCATCAACCCTGTCCCGCCATTGCTGTCGAACTCACGGGTCCGTCTCGGCAGCCTCTCGGACCTTGCAACCCCCTg                                     >  2:32305/4‑298 (MQ=11)                                                                                                                                                                                                                                                                   cGGTGTCGATTTCTTTCTGAGGCCCCGGGATCGCCACCTGGACGGCGCCGGTTTCGCCGGGATCGTCGCGGTGGTAAGAGCCGGCAACACGGATCGGCACAGCGTCCACACCGGTTGCGGCGTAAAGCTCCCAGATAGCCGAATCCGGGAAGCCACCGAGCGACCACTCCATGGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATCCCCGCACCGCGATAGTTTTCGAGCTTACGGGTCAGTTTTGGCAGCGTGACGGACTTTGCAACGCCCTGATAGCt                               <  2:389253/298‑1 (MQ=11)                                                                                                                                                                                                                                                                           aTTTCTTTCTGACGTCCACGCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATCCCCGCACCGCGATAGTTTTCGAGCTTACGGGTCAGTTTTGGCAGCGTGACGGACTTTGCAACGCCCTGATAGCTGTAGCCGt                       >  1:506698/1‑298 (MQ=14)                                                                                                                                                                                                                                                                                              gCATGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATCCCCGCACCGCGATAGTTTTCGAGCTTACGGGTCAGTTTTGGCAGCGTGACGGACTTTGCAACGCCCTGATCGCTGTAGCCGTTCAGAAAACCGTTCATTaa    >  2:296373/1‑298 (MQ=9)                                                                                                                                                                                                                                                                                                aTGACCACCTCGACGGCCACCGTTTCGCCGGTATCGTCGCGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATCCCCGCACCGCGATAGTTTTCGAGCTTCCGGGTCCGTTTTGGCAGCGTGACGGACTTTGCCACGCCCTGATCGCTGTAGCCGTTCAGAAAGGCGTTCGTTAACt  >  2:272367/1‑298 (MQ=9)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                                  TTGTCCAGGGTAATCACATTTTCTTTGTTCATGGTTTTCTCACTTCTGAATCGGGGTTAACCGGTCAGCCAGGCTGACCGGATGAAAATCACAGGCCGATATTGCGGCGGTGTTGCTCCAGCCGGTCGACGCCGTTCACCTTCTCAATCATGTTGATGGTGTCGATTTCGACCAGCTCCTTACCGTCCATCGTCAGCCGGAAATAGGTGCAGACCACGGAGATTTTCGACTCGGTGTCTTCTCCCTGTTTGCCCTCGCCGGTGTCGATTTCTTTCTGACGTCCACGCATGACCACTTCGACGGCCACCGTTTCGCCGGTATCGTCACGCTGGTAAGAGCCTGCAAAACGAATCGGCACAGCATCCACACCGGTTGCGGCGTAAAGCTCCCAGATAACCGAATCCGGGAAGCCACCGAGCGACCACTCCATTGACAGCGCATCGTCATCAAGGCCGAGGTCTACCGGTGCGCTGCCGTTCATCCCCGCACCGCGATAGTTTTCGAGCTTACGGGTCAGTTTTGGCAGCGTGACGGACTTTGCAACGCCCTGATAGCTGTAGCCGTTCAGAAAGACGTTCATTAACT  >  CP006584/2251291‑2251875

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑