Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006584 3,278,551 G→A W246* (TGG→TGA)  LY180_15575 → aldehyde oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0065843,278,5510GA93.9% 145.4 / NA 51W246* (TGG→TGA) LY180_15575aldehyde oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base A (21/25);  total (23/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

GGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGGGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGA‑ACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCC  >  CP006584/3278258‑3278832
                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                          
ggCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTgagggg                                                                                                                                                                                                                                                                                        >  1:353009/1‑298 (MQ=255)
      gTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGc                                                                                                                                                                                                                                                                                  <  1:437998/298‑1 (MQ=255)
      gTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGc                                                                                                                                                                                                                                                                                  >  1:577499/1‑298 (MQ=255)
        gATCGCCCGTAAAACCACCGGCGACCAGCAGGGGTTCCATTCTGCCCCTGTTCAGCCGGTATTTGCCGTGCGCGGACCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAg                                                                                                                                                                                                                                                                                <  2:295228/298‑1 (MQ=255)
           ctcCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGgcg                                                                                                                                                                                                                                                                             >  1:396299/1‑298 (MQ=255)
                     aacgcaGGCGACAGGCAGGCGTTTCATTCTTCCCATGGTCAGGCGGGATTTGCCGTGCTCGAGCCGGGTTATACCTACAACCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGGACACACCGTGGAACAGTATGTTAACAAACCGGTTGATGCCAAAATGCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGGCCGAAAGCCCTGAAAGAGCCAGAAGACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGtt                                                                                                                                                                                                                                                                   <  2:28806/294‑1 (MQ=255)
                     aCCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCCGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCCACGCCGGCGCTGCACGGtt                                                                                                                                                                                                                                                                   >  1:170791/1‑298 (MQ=255)
                            gCGACAAGCAGACGCTTCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTATATACCTACACTCTGCCGCCGCGTCAGGTGGCTAACGGCCTAGTGGACCCGTTTGTACCCACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATCCAGGACCGTCTCGCCTCAGCCACTATGCTGAGCCTAGTCACAGACGTACCGAAAACCCAGCAGCAGCCAGAAACATACATAGCGTGCACCAACGTCATGACGGGGGCCAAGCTTGCGCTGagaggtgtccgtgc                                                                                                                                                                                                                                                            >  2:225763/1‑285 (MQ=255)
                                 aaGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTg                                                                                                                                                                                                                                                       >  1:500670/1‑298 (MQ=255)
                                  aGCAGGCGTTCCGTCCGTCCCATGTGGCGACGGTATTACCAGTGACCGCTCGCGTGTATCCTTGCACCCTGGCGCCGCGTTGGGCGGCGAACGGGGGAGGTGGCGCCTTTGTCCAGCGCGGTGAACGGGATGTTACCACACCGGTTGATGCCAAAATTCAGGACAGTTTCGCAGAAGGCAGGTTGCTGCGGCTGACCGAAGATGGGCCGAACGCCCTGGAAGAGCCCGCAACATACGGTGTGCGCGCAAACGTCCTGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTgg                                                                                                                                                                                                                                                      <  2:237487/298‑1 (MQ=255)
                                    cAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCg                                                                                                                                                                                                                                                    <  1:142624/298‑1 (MQ=255)
                                      ggCGGTCCATGCTGCCCGTGTTGAGCCGGTATTTGCCGTGCGCGATCCGGTTTATGCCTACACCCGGCCGCCGCGTCAGGGTGCTAACGGCGGAGTGGACGCCTTTGTACACACCGTGGAACAGTATGGTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGAGGGTCCGAAAGCCCTGAAAGAGCCAGAAAGCTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTa                                                                                                                                                                                                                                                  <  2:140228/298‑1 (MQ=255)
                                          ttCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGc                                                                                                                                                                                                                                              >  1:532884/1‑298 (MQ=255)
                                             cATTCTGCCCATGTTCAGCCGGTCTTTGCCGTGCTCGATCCGGTTTATACCTACACCCTCCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGTTGCCAAACTTCCGGACCGCTTCGCAGACGGCATTTTGCTGACGCTGATCGACGAGGGTCCGCAAGCCCTGACAGAACCAGCAAACTACGCAGCGAGCGCGAACGTCATGTGAGGGGCGCCAACGGCGGTGAACGGCGTGATTGGCGCCTGCGCTCCGCacg                                                                                                                                                                                                                                           >  2:559480/1‑296 (MQ=255)
                                                ccTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACt                                                                                                                                                                                                                                        <  1:342439/297‑1 (MQ=255)
                                                  tGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGCGGGGCGACGCAGGCGCTGACCGGTTTGCTTGGCGCTGGCGTCCCGCCGGACTgg                                                                                                                                                                                                                                      >  1:469491/1‑298 (MQ=255)
                                                                       ttcattgttCGATTCGGTTTATCCCACCCCCCTGCGCCCGGGTAAGTTGGCTAAGGGCCTAGTGGCCGCCTTTGGACAAACCGTTGCACAGTATGTTACCAAACCGGTTGAGGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCAc                                                                                                                                                                                                                 <  2:396299/290‑1 (MQ=255)
                                                                                       cttttctccccgtcccTTCCTCCGCGGCTGTTCGCTACCGGCGTGTTGTCCGCCTTTTTTCACCCCTTGTGACATGATGTTACCAACCCGGTGGCGGCCAAACTCCAGGACCGTTTCGCGGAAGGCATTTTGCTGCCGCTGACCGAAGATGGCCCTATAGCCCGGAAAGAGCCAGACAACTTCGATGTGCGCACCAACGTCATGTGCGTGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGTACTGGGCCACGCATATGCTGGGCCACTCTCTGACTGCGTTGc                                                                                                                                                                                                 <  2:243941/285‑1 (MQ=255)
                                                                                         ttatgccctcacCCTGCCGCCGCGTCTGGGGGCGAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTGTGTTACCAAACCGGGTGATGCCAAAAATCAGGACCGTTTCGCAGAAGGCATTTTGCGGACGCGGATCGAAGATGTGCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGGGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCAc                                                                                                                                                                                               <  2:152845/289‑1 (MQ=255)
                                                                                           ataCCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTGTGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCAAGAACTGACTGCGATGCACga                                                                                                                                                                                             >  1:152845/1‑297 (MQ=255)
                                                                                                     ccTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCAc                                                                                                                                                                                   <  1:587421/298‑1 (MQ=255)
                                                                                                                gTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACt                                                                                                                                                                        <  1:567875/298‑1 (MQ=255)
                                                                                                                           aaCGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTcc                                                                                                                                                             >  1:636939/1‑298 (MQ=255)
                                                                                                                                  tAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGCAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGACGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACCTACTTCCTGCCATGCCAGGTCAGGACCAACCCCCACAACAGGCTACCGTcctgcaccc                                                                                                                                                      >  2:556883/1‑294 (MQ=255)
                                                                                                                                      ggcagCCTTTGTACACACCGTGTAACATTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACtg                                                                                                                                                  <  1:186760/294‑1 (MQ=255)
                                                                                                                                         cGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTgg                                                                                                                                               >  1:279547/1‑298 (MQ=255)
                                                                                                                                           ccTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGaa                                                                                                                                             >  1:175990/1‑298 (MQ=255)
                                                                                                                                                  acacacCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGaaaaa                                                                                                                                      <  1:639374/298‑1 (MQ=255)
                                                                                                                                                           ggAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGAcc                                                                                                                             >  1:461594/1‑298 (MQ=255)
                                                                                                                                                            gAACAATATGATACCAACCCGGTTGATGCCAAAACTAGGGAACGTGTCGCAGAGGGGATTGTGCTGACGCGGGGCGAAGATGGGCCGAAAGCGCTGAAAGAGCGAGAAAACTGCGATGTGCGCGCCATGTTCATGGGAGGGGCGACGGAGGCGCTGGAGGGTTGGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACGGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCa                                                                                                                            <  2:466324/298‑1 (MQ=255)
                                                                                                                                                                aggaTGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAgcg                                                                                                                        <  2:175990/295‑1 (MQ=255)
                                                                                                                                                                aGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAgcg                                                                                                                        >  1:599142/1‑298 (MQ=255)
                                                                                                                                                                 gTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAgcgc                                                                                                                       <  1:260725/298‑1 (MQ=255)
                                                                                                                                                                         cccaaCCGGTTGGTGCCAAAATTCAGGACCGGGTCGCAGAAGGCATTTTGCTAACGAGGATCGAAGAGGGTCGGAAAGCCCTGAAAGAGCCAGAAAACGACGAGGTGCTGGGCAACGTCGTGTGGGGGGGGACGACGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGGCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAgct                                                                                                               <  2:532884/295‑1 (MQ=255)
                                                                                                                                                                         ccAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAgct                                                                                                               <  1:50352/298‑1 (MQ=255)
                                                                                                                                                                                              ttCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAAAGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGATTGGCCCCGAACTGACTGCGATGCCCGGTCTGGACCACGCCCCACCACTGGCTAACGTCCTCCCTGCAATCTGGAATGCAAAACGCGAGCCCAAGCGCCCACAGCCGCTGCCatattgagaacggcg                                                                                          >  2:216062/1‑287 (MQ=255)
                                                                                                                                                                                              ttCAGAGCCGTTTGGCAGTAGGCATTTTGCTGACAGTGATCGAAGATGGGCCGAAGGCCCTGAAAGAGCCAGAAAACTACGATGGGCGCGCAAACGTCAGGTGAGGGGCGACGCGGGCGCTGAGCGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGt                                                                                          <  2:616923/298‑1 (MQ=255)
                                                                                                                                                                                                 aGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTg                                                                                       <  1:364921/298‑1 (MQ=255)
                                                                                                                                                                                                                aaGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGA‑ACATCACTGAAgg                                                                       <  1:79425/298‑1 (MQ=255)
                                                                                                                                                                                                                           cTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCCAAACTGCTTCAATCTGCTAAACCCGTCCGGACACA‑CCCTGAAGGTTCCgatggct                                                            >  2:270199/1‑295 (MQ=255)
                                                                                                                                                                                                                            tGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGA‑ACATCACTGAAGGTTCCGAtgatga                                                           <  1:421725/298‑1 (MQ=255)
                                                                                                                                                                                                                            tGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGCCTATCGTCCTGCCTGCACTGTGGAATGAAAAACCCCAGACCAAGCCCGCTAACCTGCTGCACTATGCGCAACGCGCCCGGA‑ACATCACCGAAGGGCCCGAtgctga                                                           >  2:511735/1‑298 (MQ=255)
                                                                                                                                                                                                                                   gATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGA‑ACATCACTGAAGGTTCCGATGATGAGCGTAtt                                                    <  1:225763/298‑1 (MQ=255)
                                                                                                                                                                                                                                               tCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGCAGCACGGCCTGGATCACCCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAACGAAAAACGCACGACCCCGCGCCCGAAGCTCCTCCAATATGCTTCACCCCACCGGA‑ACATCACCGAAGCATCACATGATCAGCCTATTCAcacctgcctt                                        >  2:12805/1‑292 (MQ=255)
                                                                                                                                                                                                                                                ccGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGA‑ACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTg                                       >  1:336877/1‑298 (MQ=255)
                                                                                                                                                                                                                                                 cGAAAGCCCGGAAACGCACAGAAAGCGACGTGGTGGGCGCCAACGTGAGGGGGGGGGCGACGCAGTCGCTGAACGGTTTGAGTGGCGCTGGCGTACCGCAGGCCTGGGCAACGCATATGCTGGGCCACGAACTGGCTGCGAGGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGA‑ACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGc                                      <  2:370885/298‑1 (MQ=255)
                                                                                                                                                                                                                                                   aaaGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGA‑ACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCg                                    >  1:122233/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                  ccAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGA‑ACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCt                     >  1:369785/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                                 gtgCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGA‑ACATCACTGAAGGGTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTCAGCAATTAGGCg      >  1:152228/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                                  tgCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGA‑ACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGt     <  1:467017/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                                    cgcgcCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGA‑ACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGc   <  1:270774/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                                     gcgcCAACGTCAGGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGA‑ACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGcc  <  1:32284/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                                                          
GGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGGGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGTACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAACGCGTCTGGA‑ACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCC  >  CP006584/3278258‑3278832

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑