| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006584 | 1,324,434 | A→C | K833T (AAA→ACA) | narZ → | nitrate reductase A subunit alpha |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006584 | 1,324,434 | 0 | A | C | 98.0% | 171.9 / NA | 51 | K833T (AAA→ACA) | narZ | nitrate reductase A subunit alpha |
| Reads supporting (aligned to +/- strand): ref base A (1/0); new base C (23/27); total (24/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.71e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01 | |||||||||||
ACGGAGCACGGTATCCAGGGTAAAGATCTGGGGCAACAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCT > CP006584/1324139‑1324728 | aCGGAGCACGGTAGCCAGGGTAAAGATCTGGGGCAACAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACAtt < 1:550735/299‑1 (MQ=255) ggAGCACGGTATCCAGGGTAAAGATCTGGGGCAACAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGACAAAGACGACATGAATACTTCGGATATGAATCCGTTTATTCCCCCGCTGTCGTCGGCGGTCGCACCGGCGTGGGCAGCGACAGGCGAGTGGGAAATTTACAAAGCCATCGCGAAGACAttag > 2:308351/1‑297 (MQ=255) ccAGGGTAAAGATCTGGGGCAACAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCg > 1:382254/1‑299 (MQ=255) cAGGGTAAAGATCTGGGGCAACAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGt < 2:161955/299‑1 (MQ=255) aGGGTAAAGATCTGGGGCAACAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTc < 1:28420/299‑1 (MQ=255) ggTAAAGATCTGGGGCAACAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGCCGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCCCCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAACTTTACACACACAACGCGACGACATTCCCCGAAGTGCGCGCCgg > 2:618102/1‑299 (MQ=255) cAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAAc > 1:455766/1‑299 (MQ=255) tGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTc > 1:273835/1‑299 (MQ=255) tGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTc > 1:310284/1‑299 (MQ=255) aaGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCAc < 1:447206/299‑1 (MQ=255) ccGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCt < 1:353751/299‑1 (MQ=255) cGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTg > 1:2405/1‑299 (MQ=255) aagaagTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCt < 1:65752/299‑1 (MQ=255) tGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAg < 1:132571/299‑1 (MQ=255) gCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGCAAACGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAGAGCGACTGGGAAAGTTACAAAGCCATCGCGAATACATTCCCCGAAGTGTGAGTAGGTCATCTGTGTAAAGAAACCGAACTCGTACCGATGCCTCCACAGCCCGAcc > 2:98298/1‑298 (MQ=255) aCAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGcc < 1:649639/299‑1 (MQ=255) aCAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGcc > 1:165589/1‑299 (MQ=255) cAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCg < 1:450736/299‑1 (MQ=255) ctggtaaggtgaGCTGGGTCGGTTGGGTAGGGTGGACTTCGGTCTGTC‑AGTACCCGGGTCATATTCCGACAGAGTTTTGGGGGAGGAGACCTGGTGCGAAGAAGACGACATTAATACTGCGGTTATGCATCCGTTTATGAACCCGCGGTCGGCGGCGGTCTATCCGGCCGGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAAc < 2:655639/288‑1 (MQ=255) ggaGGCGAGCTGGTTCTGGTGTTCACGCTGGTCTTCCGGCGGGCGAGCAGCGGTCTC‑TATTCCGACATCATTGTGCCGACGGCGACCTGGTACGAAACAGACGAAAGGAAGACTTCGGAGATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGgc < 2:455766/297‑1 (MQ=255) cAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGc > 1:38155/1‑299 (MQ=255) aTCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGAt > 1:258192/1‑299 (MQ=255) cTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGaa < 1:273057/299‑1 (MQ=255) cTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAg < 1:607993/299‑1 (MQ=255) tCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGc < 1:444324/299‑1 (MQ=255) gTCTGTCGAGCACCTGTCTC‑TATTCCGACATCCTTTTGCCGAAGCCGACCGGGTCCGAACAAGACGACATGAAGACTTCAGATATGCATCCGTTTATTAACACGCTGTCTGCGGCGCTCGATCCGGCTTGGGAATCGAAAAGAGACTGGGAAATGTACAACCCCATCGCGAAGCAGTTCTCCGAAGGTTACGTCGCACATCTGGGCAATGACACCGACTTCACACCGCTCCCCACCCCGCCCGACTCTGCCTCTGCACCGCCTACGCCCCTGTATGTCCACGACTGGAAAAAAGCCGaa > 2:619369/1‑298 (MQ=255) gCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTg > 1:474583/1‑299 (MQ=255) cttcaTTCCGGTATCATTTTGCCGACGGCGACGTGGTAAGAAATAGCGGATATGAGTACTGCGGATAGGCATCCGTTTATGCACCCGCTGTCTTCGGCGGGCTATCCGGCCTGGGAATCGAAAAGCGAATGGGAAATTTAAAAAGCCATCGCGAAGACATTCTACGAAGTGTGCGTCGGTCAGCTGGGTAAAGAAACCGGCATCGGCACGCTGTCTAGCCAGCACGAGTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTgg < 2:512426/295‑1 (MQ=255) cGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTc < 1:527434/299‑1 (MQ=255) ggCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCg < 1:537659/299‑1 (MQ=255) acccgggTACGAAACAGACGGCATGAATCCTTGGGTTATGCATCCGTTTATTCGCCCGCTGTCTGGGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTag < 2:246707/294‑1 (MQ=255) cTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAgc > 1:267790/1‑299 (MQ=255) cTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTATGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAgc < 1:49533/299‑1 (MQ=255) acgacATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCg < 1:308351/299‑1 (MQ=255) gttggATGTGCTGCCGTTTATTCACCCGCGGTCGGCGGCGGCCGAGCCGGCCTGGGAGGCGAAAAGGGACTGAGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGGAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCt < 2:2405/296‑1 (MQ=255) tgttggctgtttttCCCCCGCGGGGTGGGGGGGTCGATCGGGCCTGGGAAGAGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAGGTGTGCGTCGGTCATCTGGGTAAAGCAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACctct < 2:38155/287‑1 (MQ=255) cATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGCCTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTACAGAAACCGACGTCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAAATCGCGCCGCCGCCGGATGTGCAAGACTGGAAAAAAGGCAAGTGCGAGCTGATCCCTGGTCAAACACGC‑CAACACATTAAGGTCGACGAGCGTGATTTTACGGCGACATACAGACGCATTAccgcgct > 2:126764/1‑294 (MQ=255) cATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGGTTTACCTCTAt > 1:10525/1‑299 (MQ=255) cGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGCAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCCCGCTGCCTATCCAGCACGAATCTGCAGATGAACTGGCGCAGCCGCCGGATGTGAAAGACTGGAAAAAAGGCATGTGCGACCTGCACCCTGGAAAAAC‑CCAGCCCCCCATTAGGTACTGAGCGCGGGAGTATCGTGCGCCTTCCAAAAGCTTTACCTCTacgtcc > 2:599066/1‑294 (MQ=255) tATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGc > 1:290525/1‑299 (MQ=255) attcccgctcggTCGGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTTGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGCGCGTCGGTCTTCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCt < 1:274249/288‑1 (MQ=255) ttCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGGGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTg < 1:483544/299‑1 (MQ=255) ccGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGga > 1:133647/1‑299 (MQ=255) cTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTCGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGTGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGTAGaa > 1:411887/1‑299 (MQ=255) aTCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGt < 1:174775/299‑1 (MQ=255) gacacaagggaccTGGGAAATTTACCAACACATCGCGAAGACATTCTCCGTGATGGGCGGCGGCCATCTGGGGAAAGAACCCGACAGCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCt < 2:290525/287‑1 (MQ=255) aaaGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAac > 1:286159/1‑299 (MQ=255) aaGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCt > 1:533957/1‑236 (MQ=255) aaGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCt < 2:533957/236‑1 (MQ=255) aGCGACTGGGAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCAACACCTTATGGTCGAAGCCGGCGCTCATCCGGCGACTTACTAACGCTTTACCTCTAACGGCCCGCCTACGGCGAAAATCGGTAAAGGCGGTAAAGGGCTTTCCTCGTacac > 2:293074/1‑299 (MQ=255) gAAATTTACAAAGCCATCGCGAAGACATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCga > 1:231452/1‑299 (MQ=255) aagacATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCt < 1:162770/299‑1 (MQ=255) | ACGGAGCACGGTATCCAGGGTAAAGATCTGGGGCAACAGGGCGGCGTGAAGCCGGAAGAAGTGGACTGGCAGGACAATGGTCTGGAAGGCAAGCTGGATCTGGTGGTTACGCTGGACTTCCGTCTGTCGAGCACCTGTCTC‑TATTCCGACATCATTTTGCCGACGGCGACCTGGTACGAAAAAGACGACATGAATACTTCGGATATGCATCCGTTTATTCACCCGCTGTCTGCGGCGGTCGATCCGGCCTGGGAAGCGAAAAGCGACTGGGAAATTTACAAAGCCATCGCGAAGAAATTCTCCGAAGTGTGCGTCGGTCATCTGGGTAAAGAAACCGACATCGTCACGCTGCCTATCCAGCACGACTCTGCCGCTGAACTGGCGCAGCCGCTGGATGTGAAAGACTGGAAAAAAGGCGAGTGCGACCTGATCCCTGGTAAAAC‑CGCGCCACACATTATGGTCGTAGAGCGCGATTATCCGGCGACTTACGAACGCTTTACCTCTATCGGCCCGCTGATGGAGAAAATCGGTAATGGCGGTAAAGGGATTGCCTGGAACACCCAGAGCGAGATGGATCTGCTGCGTAAGCT > CP006584/1324139‑1324728 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |