Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	1,459,895	C→T	T49T (ACC→ACT)	LY180_06935 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	1,459,895	0	C	T	98.3%	191.2 / NA	60	T49T (ACC→ACT)	LY180_06935	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (34/25);  minor base A (1/0);  total (35/25)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

ATGAAAACATTGCTCGGCGAGAGGATTATGGATCGCATGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACCTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACTGCATGCCGGGCGGTAATTTACCAGCAGAGCCGAAAGCTG  >  CP006584/1459607‑1460190                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                        aTGAAAACATTGCTCGGCGAGAGGATTATGGATCGCATGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGCATTGCGAAGTAATTTGTACCGGTAGAAAAATTTAATGGAGACCGTTTTTGACGCACTGAAAGCAATGGGAACAGCAACCTCCATAGAAACTGCTGCGCGAACTCATATCAGCCGTAAAGAAGT‑GCTGAACGAAACGTGAGAACTAAAAAAACGTGGTTTAGTTGCTAAAAGACCGTAGACTTggggcgtag                                                                                                                                                                                                                                                                                               >  2:395889/1‑292 (MQ=255)   gAAAACATTGCTCGGCGAGAGGATTATGGATCGCATGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCt                                                                                                                                                                                                                                                                                             <  1:534069/299‑1 (MQ=255)         aTTGCTCGGCGAGAGGATTATGGATCGCATGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTGTCGTTGATAAAAGCGAGTACACTTGGCGTGTGGCTGATaac                                                                                                                                                                                                                                                                                       >  1:486164/1‑299 (MQ=255)             cTCGGCGAGAGGATTATGGATCGCATGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACg                                                                                                                                                                                                                                                                                   >  1:377071/1‑299 (MQ=255)             cTCGGCGAGAGGATTATGGATCGCATGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACg                                                                                                                                                                                                                                                                                   >  1:272592/1‑299 (MQ=255)               cGGCGAGAGGATTATGGATCGCATGACCATGATCGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGtt                                                                                                                                                                                                                                                                                 <  1:150854/299‑1 (MQ=255)                  cGAGAGGATTATGGATCGCATGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAACCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTcag                                                                                                                                                                                                                                                                              >  1:584019/1‑299 (MQ=255)                     gagGATTATGGATCGCATGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTcagcag                                                                                                                                                                                                                                                                           <  1:241450/299‑1 (MQ=255)                                aTCGCATGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGCATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGAGAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAAcagccagc                                                                                                                                                                                                                                                                >  2:557836/1‑299 (MQ=255)                                  cGCATGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGACGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGAGCGTGCATTAAAACAACGTTAAGCAAGGAcacacagcca                                                                                                                                                                                                                                                              >  2:222845/1‑297 (MQ=255)                                     aTGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCgg                                                                                                                                                                                                                                                           >  1:76234/1‑299 (MQ=255)                                     aTGACCAGGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCgg                                                                                                                                                                                                                                                           <  2:76234/299‑1 (MQ=255)                                       gACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGGCGGCa                                                                                                                                                                                                                                                         >  1:586425/1‑299 (MQ=255)                                          cATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGaa                                                                                                                                                                                                                                                      >  1:624044/1‑299 (MQ=255)                                                        gATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAAc                                                                                                                                                                                                                                        >  1:111813/1‑299 (MQ=255)                                                         aTGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTTGTCTAGCCAGGAATTTCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAAACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACt                                                                                                                                                                                                                                       <  1:65591/299‑1 (MQ=255)                                                                    ttAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAACAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGACCTGAAAACGGCTGGTTTCGTTGATACAAGCGCGTACACTTGGCGTGTGGCTGATAACACCGTTCAGCAGGAACAGCCAGCGCCGGCAGCACTGCCGGAAGAAACCACCACGGCacc                                                                                                                                                                                                                            >  2:465800/1‑297 (MQ=255)                                                                      aaCTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAg                                                                                                                                                                                                                          <  1:282834/299‑1 (MQ=255)                                                                             agagCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCGGGAACAGCCAGCGCCGGCAGAACTGCCGGACGAAACTACCACGGCAACCGTCGCaaa                                                                                                                                                                                                                   >  2:594119/1‑299 (MQ=255)                                                                                               tcgtcCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGAttt                                                                                                                                                                                                 <  1:277819/299‑1 (MQ=255)                                                                                                   ccagccagGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACt                                                                                                                                                                                             >  1:622225/1‑299 (MQ=255)                                                                                                       ccagGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGcga                                                                                                                                                                                         >  1:255184/1‑299 (MQ=255)                                                                                                           gAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCTTACACTTGGCGCGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGCACGAAACTACACCGGCAACAGTCGCGAAAATTTCGGCAAGCGATATAACCGCAACCAt                                                                                                                                                                                     >  2:226289/1‑299 (MQ=255)                                                                                                                  gAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGaacaa                                                                                                                                                                              >  1:114592/1‑299 (MQ=255)                                                                                                                    aGTAATTTTTACCGTGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACg                                                                                                                                                                            <  1:309501/299‑1 (MQ=255)                                                                                                                                         aaTTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGACCTATGGCCACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGCACTGCCGGAAGAAACTACCCCGGCAACAGACGCCAAAATTTCAGACAGCGATTTAACCACAACCATCGAACAACACCGTCCACTAAcacagccaga                                                                                                                                                       >  2:404094/1‑290 (MQ=255)                                                                                                                                               ctGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAACAGCGCGTAGACATGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCAAGCGCCGGCAGAACTGCCGGAAGCAACTACCACGGCAACAGTCGCGAAAATTTCAGAACACAATTTAACCGCGAAGAATGAACAACCAGGTCCACTAACTGCGTATGacatcac                                                                                                                                                 >  2:597562/2‑293 (MQ=255)                                                                                                                                                         tttttGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTc                                                                                                                                       <  1:477238/299‑1 (MQ=255)                                                                                                                                                                       aagagaaTGAGAAAAGCCACAAGCGTAGAACATGCGGGTGGACGTGTTATCAGACGTGGAGAAGT‑GCTGAACGAACTATGGGAGATGAAGAAGGCTGGTTTCGTTGACAAAAGCGCGTACACTTGGCGGGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCg                                                                                                                         <  2:295619/294‑1 (MQ=255)                                                                                                                                                                       aaaGCAAGGGGAAAAGCCACACCCCTAAAACTTGCTGCGCGACTTGATATCAGCCGGGAAGAAGT‑GCATAACGAACTATGGGAACTGAAAGAGGCTGTTTTCGTTGATAAAAGCGCGTACCCTTGGCGTGGGGCTGATAACAACGGTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCg                                                                                                                         <  2:624044/299‑1 (MQ=255)                                                                                                                                                                               gggAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATACAAGCGCGTACACTTGGCGTGCGGCTGATAACAACGTTCAGCAGGACCAGCCAGCGCCGGCAGAACTGCAGGAAGCACCTACCAAGCCAACAGTCGAGAAAATTCCAGCGAGCGATTTAACCACGACGATTGAAAAACGTCGTCCACTCACCGCGGATGACCTGCAGACAGTGTTCGCTACCACATCCCGCaacttgt                                                                                                                 >  2:28783/1‑294 (MQ=255)                                                                                                                                                                                   aatgacacaTCCATAGAACTTGCTGTGCGACGTGGTAGCAGCCGTGAAGAAGG‑GGTGAACGAAGTAGTGGAACTGAAAAAGGCTGGTTTAGTTGATAAAAGCGCGTACACTTGGCGTGTTGCTGATAACAACGTGCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACCACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTc                                                                                                             <  2:15458/294‑1 (MQ=255)                                                                                                                                                                                        cacaTCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTCCCACATCCCGCAAAGTAGCTTCAACGc                                                                                                        >  1:293684/1‑299 (MQ=255)                                                                                                                                                                                             ccATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCtggca                                                                                                   >  1:238035/1‑299 (MQ=255)                                                                                                                                                                                                gaGAACTTGCGGGGCGACTTGATATCAGCCGTGAA‑AAGTGGCGGAAAGAACTAGGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGGGCGGCCACTTGGGTGGGGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCtggcaatg                                                                                                <  2:377071/298‑1 (MQ=255)                                                                                                                                                                                                   aaCTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCACCTAACTGCGGCTGAACTGGCCACGTTGTTCGGTACCACATCCCGCAAAGTGGCTCTAACGCtggccattgca                                                                                             >  2:518702/1‑299 (MQ=255)                                                                                                                                                                                                         ctgcGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGc                                                                                       <  1:637147/299‑1 (MQ=255)                                                                                                                                                                                                                gattGGTATCAGCCGTTAAGAATT‑GCTGAACGAACGATGGGAACTGAAAAAGGAGGGTTTAGTTGATAAAAGCGCGGACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGGGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGAGTGAACTGCGACGAGTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTc                                                                                <  2:323865/297‑1 (MQ=255)                                                                                                                                                                                                                              gTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACGACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGtt                                                                  <  1:507117/299‑1 (MQ=255)                                                                                                                                                                                                                               tGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTCCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTa                                                                 >  1:104491/1‑299 (MQ=255)                                                                                                                                                                                                                                       t‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAAc                                                         >  1:641383/1‑299 (MQ=255)                                                                                                                                                                                                                                       t‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAAc                                                         <  1:518702/299‑1 (MQ=255)                                                                                                                                                                                                                                         gCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACTTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACg                                                        <  1:263818/299‑1 (MQ=255)                                                                                                                                                                                                                                          cTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACgg                                                       >  1:550757/1‑299 (MQ=255)                                                                                                                                                                                                                                            gaacgaacTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTa                                                     <  1:650418/299‑1 (MQ=255)                                                                                                                                                                                                                                            gaacgaacTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTa                                                     <  1:594119/299‑1 (MQ=255)                                                                                                                                                                                                                                              acgaacTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTaaa                                                   >  1:273537/1‑299 (MQ=255)                                                                                                                                                                                                                                                gaacTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAAtt                                                 <  1:557836/299‑1 (MQ=255)                                                                                                                                                                                                                                                         gAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACTg                                        <  1:222845/299‑1 (MQ=255)                                                                                                                                                                                                                                                                   aGGCTGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACTGCATGCCGGGc                              >  1:137609/1‑299 (MQ=255)                                                                                                                                                                                                                                                                       tGGTTTCGTTGATAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCAGCAAAGTGGATTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTATGATTCGTGTTAATGAGACCGGTCAATTTCGTTACTGCATGCCGGGCGGTa                          >  1:112619/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                  aTAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACTGCATGCCGGGCGGTAATTTACcagca               >  1:213740/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                   tAAAAGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCCGCAAAGCTCGTCGCATTCGCGTTAATCAGAACGGTAAATTTCGTTACTGCATGCCGGCCGGTAATTTAAcagacg              >  2:144359/1‑296 (MQ=255)                                                                                                                                                                                                                                                                                     aagacgcgTAAAGTTGGCGTGTGGCTGATAACAACGTTCCACAGGAACAGCCAGGGCCGGCAGAACTGCCGGAAGAAACTACCCGGGAAACAGGCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACTGCATGCCGGGCGGTAATTTACCAGCagag            <  2:114592/295‑1 (MQ=255)                                                                                                                                                                                                                                                                                       aGCGCGTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACTGCATGCCGGGCGGTAATTTACCAGCAGAGcc          <  1:395889/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                         cgcgTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACTGCATGCCGGGCGGTAATTTACCAGCAGAGCCGa        >  1:503002/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                           cgTACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGATACGTTGTTCGGTACCACATCCCGCCAAGTGGCTTCAACGCTGGCAATGGCACTCAGCAAAGCTCGTCTGATTCGCGTTACTCAGAACGGCAAATCTCGTTACGGCCAGGCGGGCGGAAATTTCCaagcaggcgagaaa      >  2:489105/1‑291 (MQ=255)                                                                                                                                                                                                                                                                                             tACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCaa                                                                                     <  2:12724/218‑1 (MQ=255)                                                                                                                                                                                                                                                                                             tACACTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCaa                                                                                     >  1:12724/1‑218 (MQ=255)                                                                                                                                                                                                                                                                                               cacTTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACTGCATGCCGGGCGGTAATTTACCAGCAGAGCCGAAAGCTg  >  1:262049/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                                                                        ATGAAAACATTGCTCGGCGAGAGGATTATGGATCGCATGACCATGAACGGCGGGCGATGGGTGAATTTTAACTGGGAGAGCTGGCGTCCGAATGTCGTCCAGCCAGGAATTGCGAAGTAATTTTTACCGGGAGAAAAATTTAATGGAGACTGTTTTTGACGCACTGAAAGCAATGGGAAAAGCCACATCCATAGAACTTGCTGCGCGACTTGATATCAGCCGTGAAGAAGT‑GCTGAACGAACTATGGGAACTGAAAAAGGCTGGTTTCGTTGATAAAAGCGCGTACACCTGGCGTGTGGCTGATAACAACGTTCAGCAGGAACAGCCAGCGCCGGCAGAACTGCCGGAAGAAACTACCACGGCAACAGTCGCGAAAATTTCAGAGAGCGATTTAACTGCGACGATTGAACAACGTGGTCCACTAACTGCGGATGAACTGGCTACGTTGTTCGGTACCACATCCCGCAAAGTGGCTTCAACGCTGGCAATGGCAATCAGCAAAGCTCGTCTGATTCGCGTTAATCAGAACGGTAAATTTCGTTACTGCATGCCGGGCGGTAATTTACCAGCAGAGCCGAAAGCTG  >  CP006584/1459607‑1460190

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑