Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	1,563,485	A→G	T256A (ACC→GCC)	LY180_07445 →	transposase IS609

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	1,563,485	0	A	G	98.1%	98.1 / NA	53	T256A (ACC→GCC)	LY180_07445	transposase IS609
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (26/25);  total (27/26)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAAGTGGTACATCAGTATTCAGACAGAAAGTGAAGTATCCACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGACCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGAGTACAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTACACAAGCCAGCGTTGCGCGTGCTGTG  >  CP006584/1563189‑1563731                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       ttAAGTGGTACGACAGAATTCAGACAGAAAGTGAAGTATCCACCCCGGCTCACCCTTCAGCGTCAATGGTCTGGCTGGATGCGGGCGTGGCTAAACGCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGcc                                                                                                                                                                                                                                                      <  2:213176/298‑1 (MQ=11)    aGTGGTACATCAGTATTCAGACAGAAAGTGAAGTATCCACTCCGGCTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTc                                                                                                                                                                                                                                                   >  1:501553/1‑299 (MQ=17)      tGGTACATCAGTATTCAGACAGAAAGTGAAGTATCCACTCCGGCTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAg                                                                                                                                                                                                                                                 <  1:159231/299‑1 (MQ=17)                    tCAGACAGAAAGTGAAGTATCCACTCCGGCTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCAAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGTAGAAGCAGAAACACAAAATACAGCGAATGCATTCCTGTATCGCAAATTTCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCATCAGCAAAAACCACGCaa                                                                                                                                                                                                                                   >  1:378101/1‑299 (MQ=255)                     cagacagaAAGTGAAGTATCCACTCCGGCTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAAt                                                                                                                                                                                                                                  <  1:171666/299‑1 (MQ=17)                      agacagaAAGTGAAGTATCCACTCCGGCTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATg                                                                                                                                                                                                                                 >  1:178584/1‑299 (MQ=17)                       gacagaAAGTGAAGTATCCACTCCGGCTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGa                                                                                                                                                                                                                                <  1:644678/299‑1 (MQ=17)                             aaGTGAAGTATCCACTCCGGCTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATAAAGCGACTGCCTTCCTGTATCGCAAATAACCGACGGGACTAACTTCCACAAAGAC‑CCACGCCCGCTGGCAAAAACCaacccatacacctcc                                                                                                                                                                                                                          >  2:624777/1‑285 (MQ=11)                               gTGAAGTATCCACTCCGGCTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCAtt                                                                                                                                                                                                                        >  1:112331/1‑299 (MQ=17)                                 gAAGTATCCACTCCGGCTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGa                                                                                                                                                                                                                      <  1:489154/299‑1 (MQ=17)                                     tATCCACTCCGGCTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGAt                                                                                                                                                                                                                  >  1:71315/1‑299 (MQ=17)                                          aCTCCGGCTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGaa                                                                                                                                                                                                             >  1:226875/1‑299 (MQ=17)                                           cTCCGGCTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAg                                                                                                                                                                                                            <  1:5949/299‑1 (MQ=17)                                                   cACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGGAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGCCTTAAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAATTACAGCGACTGCATTCCAGAATAGCAAATCTCCGCAGGGACTCCCCT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCCCCCAATGATAGACATTAAGACTTTAAAAGTCAGCaa                                                                                                                                                                                                    >  2:350800/1‑299 (MQ=11)                                                      ctgtccggaTCAAGGGTCGGGTGGGTGGAGGGGGTGGCTAACATCGCCACGCTGTCAGATGGAACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCGGGCGAGACTGCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTAGCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTGGAAGGTCAGCAACAt                                                                                                                                                                                                 <  2:389622/291‑1 (MQ=11)                                                      ccTTCAGCAGTAATGGGCGTGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCGTTGAGCGTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACGTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCAGTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACAt                                                                                                                                                                                                 <  2:45267/299‑1 (MQ=255)                                                        ttCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGt                                                                                                                                                                                               <  1:566033/299‑1 (MQ=17)                                                           aGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCaa                                                                                                                                                                                            <  1:504740/299‑1 (MQ=17)                                                                cAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTca                                                                                                                                                                                       <  1:202720/299‑1 (MQ=17)                                                                     gtgggggTGGGTGCTGGCGTGGCTAAACTCGCCACGCTGTAAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGACACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCATGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTcagcagg                                                                                                                                                                                  <  2:407526/296‑2 (MQ=12)                                                                                 gCTGGCGTGGCTAAACGCGGCACGGGATCAGCTGGGACAGTACTTGAGTCTGTAAGCAGTTTTCAGGAAATCCAGAAGACGCTGGCTAGACTTCATCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAGAGACAGCGACTGCATTCCTGTATCGCCAACATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTcag                                                                                                                                                                      <  2:15145/299‑1 (MQ=11)                                                                                                    ccACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTc                                                                                                                                                   <  1:494047/299‑1 (MQ=17)                                                                                                                   gCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGt                                                                                                                                    <  1:70720/299‑1 (MQ=17)                                                                                                                        gTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTaaa                                                                                                                               <  1:441661/299‑1 (MQ=17)                                                                                                                        gTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCCTCAGCAAAAACCACGCAATGCTAGGCATTGAGTAATTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGCGTACGGGCAGCCCACACGGGCGCAAGGGCCGGGCAAAACAATGTTTaaa                                                                                                                               >  2:534623/1‑299 (MQ=11)                                                                                                                          cTTTGAGCCTGTACACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCAGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCCAATACCCGCAGGGACTACCCT‑CACAAAGTCACAACGGCCTCCAGCAAAAAACACGCAATGCTAGTCACTGAGGCATTGCAGGTCAGCAAAAAGTCAAAGTCAGCAGCAGGACAGGTAAGCCAGCCGGGGCCCAATGACGGGGCAAAAGCAgggatcaacc                                                                                                                             >  2:27646/1‑291 (MQ=11)                                                                                                                                          aGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCa                                                                                                             <  1:383267/299‑1 (MQ=17)                                                                                                                                           gTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGCCTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAg                                                                                                            >  2:634255/1‑299 (MQ=17)                                                                                                                                               tCAGAAACACCAGAAGACTCTGGCGAGACTTCAGCTAAAGTTAAGCCTCAAGTTCAAATTCAGCAACAACAGGCAGAAGCAGAAACGCAAAATACAGCGTCTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCTAAATCCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCCGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTCTAAACCGTTCGATACTGGATCAGGGCt                                                                                                        <  1:394623/299‑1 (MQ=11)                                                                                                                                                        ccAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGACAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAACTATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCACCCGGGGCGCAATGTCCGGGCAAAATTCGGTTTAAACCTGTCGATACTGGAACAGGGCTGGGATGaaa                                                                                               >  2:267742/1‑299 (MQ=11)                                                                                                                                                            aagaCGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATgca                                                                                           >  1:41598/1‑298 (MQ=17)                                                                                                                                                              gaCGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGcacc                                                                                         >  1:77261/1‑299 (MQ=17)                                                                                                                                                                      cGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTCTCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCCAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGCCAAAGTCCGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGCCAAACTCAGGTTTAAACCGTTCCATCCGGGACCGGGGCGGGAACGAACTGCCCGGCCCGCta                                                                                 >  2:160872/1‑298 (MQ=11)                                                                                                                                                                      agaaaCTTCAGCCACAGGTCAGCCGCAAGGTCAAATTCAGCAACAACTGCCAGAAGCAGAAACGCAATATTCAGCGCCTGCATTCCGGTATCGCAAAGATCCGCAGGTGAGACCTG‑CACAAAGTCACAACGACCGCCAGCAAAAGCCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGGCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCtt                                                                                 <  2:601161/295‑1 (MQ=11)                                                                                                                                                                        agaCTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCACCATGTCAACGTCAGCAGCTGGTCCGGTCAGCCAGCCGGGGCGCACTGTCCGGCCAAAATCCGGATTAAACCTTTCGATACTGGACAAGGGAGGGTATGAAATgcactccaacattgc                                                                               >  2:247441/1‑286 (MQ=11)                                                                                                                                                                                acGAAAGTTAAACCGCAAGGTAAAAATCCGCAACAACTGGCAGAAGAAGAAACCCAAAATACAGCGACGGCATTCCTGGATCGAAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCAGTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCACCGCCAGCTTGAGTACAagc                                                                       <  2:41598/298‑1 (MQ=11)                                                                                                                                                                                    cAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCACCGCCAGCTTGAGTACAAGCAGct                                                                   <  1:416035/299‑1 (MQ=17)                                                                                                                                                                                            ccGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCCGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCCGGGCTGGTATGAAATGCACCGCCAGCTTGAGTACAAGCAGCTCtggcgtg                                                           >  1:48065/1‑299 (MQ=17)                                                                                                                                                                                                        aTTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCACCGCCAGCTTGAGTACAAGCAGCTCTGGCGTGGCGGTCAGGtgc                                               >  1:265976/1‑299 (MQ=14)                                                                                                                                                                                                            aGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAAGTTTCAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCACCGCCAGCTTGAGTACAAGCAGCCCTGGCGTGGCGGTCAGGtgctgcc                                           >  2:397522/1‑296 (MQ=9)                                                                                                                                                                                                                     tGGCAGAAGCAGAACCGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCAGTCAGCAAAAACCACGCAAAGATAGTCATCGAGGATGTGAAGGGCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTACACCGTTCGATACAGGATCAAGGCCGGTCTGACAGTCACCACACGCTTGAGTACAACAAGCCCTGCCCGTGCGGTCAGGGCCTTCCTGtcacccc                                  >  2:580632/1‑293 (MQ=11)                                                                                                                                                                                                                         agaagcagaaACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGACGGGGCGGAATGTCCGGGCAAAATCAGGTTTCAACCGTTCGATACTGGATCAGGGCTGGTCTGCAATGCACCGCCAGCTTGAGTCCAAGCAGCCCTGGCTTGGCGGTCAGGTGCTTGCGTTTCCGCCAGCg                              >  2:181462/1‑299 (MQ=11)                                                                                                                                                                                                                          gaagcagaaACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTAATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCCGGGCTGGTATGAAATGCACCGCCAGCTTGAGTACACGCCGCTTCGGCGTGGCGGTCAGGTGCTTGCTTTTCCCCCCGCGt                             >  2:24726/1‑299 (MQ=11)                                                                                                                                                                                                                            agcagaaACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCACCGCCAGCTTGAGTACAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTat                           >  1:145149/1‑298 (MQ=14)                                                                                                                                                                                                                            agcagaaACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCAAGCCGGGGCGCAATGTCCGGGCAAACTCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTAGGAAAGGCACCGGCAGCTTGAGTACAAGCAGCCCTGGCTTGGGGGTCAGGTTCTTGCTGTTCCGCCacggtgt                           >  2:282153/1‑293 (MQ=11)                                                                                                                                                                                                                             gcagaaACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCACCGCCAGCTTGAGTACAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTata                          <  1:350800/299‑3 (MQ=14)                                                                                                                                                                                                                              cagaaACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCACCGCCAGCTTGAGTACAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTatac                         <  1:481731/299‑1 (MQ=14)                                                                                                                                                                                                                               agaaACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCACCGCCAGCTTGAGTACAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTAtaca                        >  1:210926/1‑299 (MQ=14)                                                                                                                                                                                                                                  aaCGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCACCGCCAGCTTGAGTACAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGc                     >  1:570004/1‑299 (MQ=14)                                                                                                                                                                                                                                         aaTACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATa                                                                                                                     <  2:423882/196‑1 (MQ=14)                                                                                                                                                                                                                                         aaTACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATa                                                                                                                     >  1:423882/1‑196 (MQ=14)                                                                                                                                                                                                                                                  aCTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCACCGCCAGCTTGAGTACAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACt     <  1:247441/299‑1 (MQ=14)                                                                                                                                                                                                                                                     gCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGGCCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCACCGCCAGCTTGAGTACAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTATACAAGCCAGCGTTGCGCGTACtgtg  <  1:282153/299‑1 (MQ=14)                                                                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                       GTAAGTGGTACATCAGTATTCAGACAGAAAGTGAAGTATCCACTCCGGTTCACCCTTCAGCATCAATGGTCGGGCTGGATGCTGGCGTGGCTAAACTCGCCACGCTGTCAGATGGCACAGTCTTTGAGCCTGTAAACAGTTTTCAGAAAAACCAGAAGACGCTGGCGAGACTTCAGCGACAGTTAAGCCGCAAGGTCAAATTCAGCAACAACTGGCAGAAGCAGAAACGCAAAATACAGCGACTGCATTCCTGTATCGCAAATATCCGCAGGGACTACCTT‑CACAAAGTCACAACGACCGTCAGCAAAAACCACGCAATGATAGTCATTGAGGATTTGAAGGTCAGCAACATGTCAAAGTCAGCAGCGGGTACGGTCAGCCAGCCGGGGCGCAATGTCCGGGCAAAATCAGGTTTAAACCGTTCGATACTGGATCAGGGCTGGTATGAAATGCGCCGCCAGCTTGAGTACAAGCAGCTCTGGCGTGGCGGTCAGGTGCTTGCTGTTCCGCCAGCGTACACAAGCCAGCGTTGCGCGTGCTGTG  >  CP006584/1563189‑1563731

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑