Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006584 2,483,647 G→A P236S (CCG→TCG)  LY180_11800 ← NADH:ubiquinone oxidoreductase subunit M

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0065842,483,6470GA96.5% 182.5 / NA 58P236S (CCG→TCG) LY180_11800NADH:ubiquinone oxidoreductase subunit M
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (27/29);  total (27/31)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGGCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAG  >  CP006584/2483360‑2483941
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                       
gtAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTg                                                                                                                                                                                                                                                                                              <  1:614499/299‑1 (MQ=255)
                 cGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGaagaag                                                                                                                                                                                                                                                                             >  1:470461/1‑299 (MQ=255)
                 cGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGaagaag                                                                                                                                                                                                                                                                             <  1:454347/299‑1 (MQ=255)
                  gAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGGCGGGGCCTGAGAGTGCCCCACCGGTAGCCAGCCATGCAGCGGAACCACCCACATTTTGACCGCGAAGGCGAAGAAGAAGc                                                                                                                                                                                                                                                                            >  2:319668/1‑299 (MQ=255)
                       ccATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGAAGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGAAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAAACGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCAAGCAAGGCAGCGGAACCCCCGACATTCTGACTGCGAAGGCGATGAAGAACCCCAGc                                                                                                                                                                                                                                                                       >  2:285964/1‑299 (MQ=255)
                           gtgGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTa                                                                                                                                                                                                                                                                   <  1:319668/299‑1 (MQ=255)
                                               cGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTg                                                                                                                                                                                                                                               >  1:377126/1‑299 (MQ=255)
                                                        gTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGc                                                                                                                                                                                                                                      <  1:167455/299‑1 (MQ=255)
                                                             atatCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTAtt                                                                                                                                                                                                                                 >  1:591296/1‑299 (MQ=255)
                                                              tatCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGTCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTc                                                                                                                                                                                                                                <  1:641680/299‑1 (MQ=255)
                                                              tatCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTc                                                                                                                                                                                                                                <  1:104941/299‑1 (MQ=255)
                                                                tCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTcag                                                                                                                                                                                                                              <  1:47815/299‑1 (MQ=255)
                                                                        ggCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCtt                                                                                                                                                                                                                      >  1:648547/1‑299 (MQ=255)
                                                                        ggCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGACCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCtt                                                                                                                                                                                                                      <  1:76862/299‑1 (MQ=255)
                                                                          tgaaactcgcgcACGCAGCGGAGACGTCGCCGATAGCCCCCCGCCAGACGTGGAGTGGTGGAAACCGTGGCGGTGGGCTCGGGGAGAGCGGCAGGGAGGAACGGAGCAAAGCGTGAGCCGCAGGTTTCAGCAAGAGCGCCGCGAGGTCAACGGAAGCGGGGGTCGGGGCCTGAGAGTGCGCAGCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCGTTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCa                                                                                                                                                                                                                    <  2:13645/288‑1 (MQ=255)
                                                                                ccATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGCTTTCAGCAAGATCCCCGCCAGGTCAACGGAACCGGCGGTCGGAGCCGGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTCACTGCGAAGCGCATGAAGAAGCCCAGCATTAACAGGTATCCCACACCCCGGCACCTTGGCGTCTTCAGCAGCTCTTCagatctg                                                                                                                                                                                                              >  2:508784/1‑293 (MQ=255)
                                                                                          accgtgagaggcgCCGAGAACCCCCGGGCAGAGGGAGAGTGGGGCAAACTCGGCCGATGCGGTCGGGAACGGCGGAAGGGAGAAACGGAGCAAAGCGTAGGCGGCAGTTTGCAGCAAGAGCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGa                                                                                                                                                                                                   <  2:559016/287‑1 (MQ=255)
                                                                                           ccGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGAc                                                                                                                                                                                                  <  1:371605/299‑1 (MQ=255)
                                                                                               agaagaTGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCg                                                                                                                                                                                              <  1:508784/299‑1 (MQ=255)
                                                                                                          aTAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTa                                                                                                                                                                                   >  1:643466/1‑299 (MQ=255)
                                                                                                                        caTAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATACCCGCGAGGTCAACGGAACCGGCGGTCCTAGCCGAAGAGTGCTCATCCGGTAGCCAGCCAGGCAGCGGAACCACCGACATCTTGACTGCGAAGGCGATGCAGAAGCCCAGCACTAAAAGACTTTACACACCACTGGAACCTGGTGTATTCACCAGC‑CCTGATACGTTAGAGGTCCACACGCCGGTCCCCTGGTAGTGCACAAAAAcaa                                                                                                                                                                     >  2:634139/1‑297 (MQ=255)
                                                                                                                         aTAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCAACGACATTTTGACTGCGAAGGCGATGAAGACGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATCAACCACCTCTTAATA‑GGTGAAGTTCCAGACGCCGGACGCGCTATAGGAAACAAAAACCac                                                                                                                                                                    >  2:205907/1‑298 (MQ=255)
                                                                                                                           aGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAgcg                                                                                                                                                                  <  1:297104/299‑1 (MQ=255)
                                                                                                                              gATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGCAGCCCAGCATTAACAGGTATTCCACACCACCGGACATTGGCGTAATCAGCAGCTCGTGATA‑GTCGAAGGTCCAGACGCCGGGCGCCTTGTAGAGGACAAAAAACAACGCCa                                                                                                                                                               >  2:464082/1‑299 (MQ=255)
                                                                                                                              gATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCa                                                                                                                                                               >  1:380874/1‑299 (MQ=255)
                                                                                                                                  ggCGCAAACTCGGCCGAGGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGAt                                                                                                                                                           <  1:410466/299‑1 (MQ=255)
                                                                                                                                               ccGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATc                                                                                                                                              <  1:447509/299‑1 (MQ=255)
                                                                                                                                                 gATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCAc                                                                                                                                            >  1:77416/1‑299 (MQ=255)
                                                                                                                                                  aTGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCAAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCAcc                                                                                                                                           >  2:347888/1‑299 (MQ=255)
                                                                                                                                                       ttCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGcc                                                                                                                                      <  1:330737/299‑1 (MQ=255)
                                                                                                                                                           ggacaacgggaaGGGGGAAACCCAGCAAACCGTAGCGGGCAGCTTCCAGCAAGCACCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGGGAGTGGGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTc                                                                                                                                  <  2:591296/288‑1 (MQ=255)
                                                                                                                                                                            aaaCGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATgaa                                                                                                                 >  1:60324/1‑299 (MQ=255)
                                                                                                                                                                             aaCGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATgaag                                                                                                                >  1:105019/1‑299 (MQ=255)
                                                                                                                                                                              aCGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATgaaga                                                                                                               >  1:501312/1‑299 (MQ=255)
                                                                                                                                                                                agagcaAACCGGAGGCGTCAGTTTTAAGAAAGAGCCCCGTGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCCTCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGGAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAAc                                                                                                             <  2:591879/297‑1 (MQ=255)
                                                                                                                                                                                  agcaAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACtt                                                                                                           <  1:611731/299‑1 (MQ=255)
                                                                                                                                                                                               cGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCAGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTg                                                                                              <  1:98716/299‑1 (MQ=255)
                                                                                                                                                                                                   aGTGTTCCGCAAGGTCGCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCGGAGAGTGGGCAGCCGGGAGCCAGCCAGGCAGCGGAACCACGGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATAc                                                                                          <  2:648547/299‑1 (MQ=255)
                                                                                                                                                                                                        tCAGCAAGATCCCCGCGGGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGtt                                                                                     <  1:614323/299‑1 (MQ=255)
                                                                                                                                                                                                              aGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCg                                                                               >  1:522719/1‑299 (MQ=255)
                                                                                                                                                                                                              aGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCg                                                                               >  1:332068/1‑299 (MQ=255)
                                                                                                                                                                                                               gATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGt                                                                              >  1:317821/1‑299 (MQ=255)
                                                                                                                                                                                                                     cgcgAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCAACCACACGGGGGTAAATGAAGAAATGGGTTGCCGCCGAGTAACGCGTGTTACCGTCATAgg                                                                        >  2:339182/1‑299 (MQ=255)
                                                                                                                                                                                                                                               gAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAgg                                              >  1:624714/1‑299 (MQ=255)
                                                                                                                                                                                                                                                    tggcggTGCGCCACCGGTGGCGAGACATGCGGCGGAACCACCGGCATTTTGACTGCGAAGGCGATGAAGAAGCCCCGCATTAACAGGGATGCCACACCGCTGGACATTGGCGTATTCAGCAGCTCTACATA‑GTTGAAGGTCCAGACGCCGGTCGCAGTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTa                                         <  2:105019/294‑1 (MQ=255)
                                                                                                                                                                                                                                                     gagaGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTAc                                        <  1:604172/299‑1 (MQ=255)
                                                                                                                                                                                                                                                       gagTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAGGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATTGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACAt                                      <  1:526328/299‑1 (MQ=255)
                                                                                                                                                                                                                                                          ggcccATCCGGTGGCCAGCCATGCCGGGGAACCAGTGGCATGTTGCCGTGGCAGGCGATTAATAAGCCCCGCATTAACGGGTATTCCACACCACTGGACAGTGGCGTATTCAGCAGGTATTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAGCTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCgg                                   <  2:377126/295‑1 (MQ=255)
                                                                                                                                                                                                                                                            cgcATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAGGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCACATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATgaag                                                                                                                <  2:224393/220‑1 (MQ=255)
                                                                                                                                                                                                                                                            cgcATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAGGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCACATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATgaag                                                                                                                >  1:224393/1‑220 (MQ=255)
                                                                                                                                                                                                                                                               aTCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCa                              >  1:57527/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                    gagaccacccaTGCAGCGGAACCTCCGACAGTTTGACTGGGAAGGCGTTGAAGAAGCCCAGGATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGCCGCCGGTCGCATTGTAGTGACCAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGcatc                         <  2:643466/295‑1 (MQ=255)
                                                                                                                                                                                                                                                                       gccagccaTGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCAtt                      <  1:339182/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                       gccagccaTGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCAtt                      >  1:124243/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                        ccagccaTGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTTAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCAttt                     <  1:464082/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                            ccaTGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCa                 >  1:193129/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                       aaCCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCagaagaagaaga      >  1:15647/1‑299 (MQ=255)
                                                                                                                                                                                                                                                                                           accGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAg  <  1:285964/299‑1 (MQ=255)
                                                                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                                                                       
GTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGGCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATA‑GTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGAAGAACAG  >  CP006584/2483360‑2483941

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑