Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	3,278,551	G→A	W246* (TGG→TGA)	LY180_15575 →	aldehyde oxidoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	3,278,551	0	G	A	98.2%	174.4 / NA	57	W246* (TGG→TGA)	LY180_15575	aldehyde oxidoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (25/30);  total (26/31)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGGGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGAC  >  CP006584/3278256‑3278835                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                               cAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTgaggg                                                                                                                                                                                                                                                                                             >  1:18776/1‑299 (MQ=255)                 ccGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTATATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAAACGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGCTGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGACGTGCTCCACAACTGCACGTGAGGGGCGAccgaagccctga                                                                                                                                                                                                                                                                             >  2:91471/1‑288 (MQ=255)                   gTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGGTACCACACCGGGTGATGCCAAAATTCCGGACCGGTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGACAGCCCTGAAACAGCCAGAAAACTAGGAAGTGCGCGCCACAGTCATGGGAGGGGCGgcgccagcccgcacc                                                                                                                                                                                                                                                                           >  2:581761/1‑288 (MQ=255)                    tAAAACCACAGGCGACAAGCAGGCGTTCCATTCCGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACg                                                                                                                                                                                                                                                                          >  1:98856/1‑299 (MQ=255)                     aaaaCCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACgg                                                                                                                                                                                                                                                                         <  1:616325/299‑1 (MQ=255)                                cggcAAGAAGGCGTTCCATTCTGCCCGTGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTCCACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGTGGCGACGCAGGCGCTGAACGGTTTGATTGgcg                                                                                                                                                                                                                                                              <  1:497609/296‑1 (MQ=255)                                     aGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAACGCCCTGAAAGAGCCAGACAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACCAAGGCGCTGACCGGGTTGCTTGGGGCTggg                                                                                                                                                                                                                                                         >  2:403192/1‑298 (MQ=255)                                        agggtgtcgatgcTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGAGCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑a                                                                                                                                                                                                                                                     <  2:146110/287‑1 (MQ=255)                                                cATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGa                                                                                                                                                                                                                                             >  1:537147/1‑299 (MQ=255)                                                        ccATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTTATTGGCGCTGGCGT‑ACCGCAGGACTGGGCaa                                                                                                                                                                                                                                     >  1:429339/1‑299 (MQ=255)                                                              tCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGAGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCata                                                                                                                                                                                                                               >  1:371598/1‑299 (MQ=255)                                                               cAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCatat                                                                                                                                                                                                                              <  1:69524/299‑1 (MQ=255)                                                                  ccGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGACCAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGCCCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGCGCCAGAAAACTACGATGGTCACGCCAACGTCATGGGAGGGGCGACGCACGCCCCGACAGGGTTGATTGGCG‑TGGCGTGACCGACGGACTGGGCaaacccggaggt                                                                                                                                                                                                                           >  2:310294/1‑289 (MQ=255)                                                                    ggTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAACTTCAGGACCGTTTCGCAGAAGGCATTTTGCTGCCGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCCGAAAACTACGATGTACGCGCCAACGGCCTGTGCGGGGGCACGCAGGCGCTGAACGGTTTGGTTGGCGCCGGCGG‑ACGGCCGGACTGGGCAGCGCagctcgtgg                                                                                                                                                                                                                         >  2:189973/1‑291 (MQ=255)                                                                     gTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTggg                                                                                                                                                                                                                        <  1:371379/299‑1 (MQ=255)                                                                              gTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAact                                                                                                                                                                                                               <  1:239518/299‑1 (MQ=255)                                                                                  tCGATCCGGTTTATACCTACCCCCTACCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGTCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAactgact                                                                                                                                                                                                           <  1:81373/299‑1 (MQ=255)                                                                                           tttATACCTACGCCCTGCCGCCGCGTCAGGGGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCAc                                                                                                                                                                                                  <  1:304738/299‑1 (MQ=255)                                                                                            ttATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACg                                                                                                                                                                                                 >  1:496681/1‑299 (MQ=255)                                                                                            tcttaCCTCCACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACg                                                                                                                                                                                                 <  1:16501/296‑1 (MQ=255)                                                                                                        ccTGCCGCCGCGGCATGTGGCTAACGGCGTAGTTGACGCCTTTGTACACACCGTGGAACAGTATTTTACCAAACCGGTTGATGCCAAAATTCAGGCCCGTTTCCCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCAcg                                                                                                                                                                                     <  1:360667/299‑1 (MQ=255)                                                                                                                 gcgTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAacac                                                                                                                                                                            <  1:65264/299‑1 (MQ=255)                                                                                                                    tCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTgg                                                                                                                                                                         <  1:346407/299‑1 (MQ=255)                                                                                                                               aggggggtggggACGGCTTTGTACACACCGTGGGCAGGTATGGTACCAAACCGGTTGAGGCCAAAATTCAGGACGGTGTCGCGGAAGGCATTTTGCAGACGCTGATCGAAGAGGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCAGGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTcctg                                                                                                                                                              <  2:663/289‑1 (MQ=255)                                                                                                                                    gTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGTGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCctgcctgc                                                                                                                                                         >  1:244167/1‑299 (MQ=255)                                                                                                                                     tattGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCa                                                                                                                                                        <  1:230031/296‑1 (MQ=255)                                                                                                                                        tGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACtg                                                                                                                                                     <  1:280530/299‑1 (MQ=255)                                                                                                                                           aCGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTgg                                                                                                                                                  >  1:215216/1‑299 (MQ=255)                                                                                                                                             gCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGaa                                                                                                                                                <  1:215532/299‑1 (MQ=255)                                                                                                                                              gtattgtataacccGTTGAACAGTAGTGTACCAAAGCGGTTGAGGCCCAGGTTCGTGAGCGTGTTGCCGAAGGTGTTATGCGGACGCGTATCGGAGATGGTCCGAAAGTCCTGAAGGGGCCATAAAACTACGGTTGGGGCGTCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTGTGATTGGGGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGaag                                                                                                                                               <  2:483713/287‑2 (MQ=255)                                                                                                                                               cTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATg                                                                                                                                              >  1:96990/1‑299 (MQ=255)                                                                                                                                                                         tcagcAAATCGGTGGAGGCGAAAATTGCGGGCCGGGTAGCGGAAGGCATTTTGCTGACGCTGATCGAAGGTGGTCCGAAAACCCTGATAGAGCCAGAAACGTACGAGGGGGGCGCAAACGTCATGTGAGGGGCGACGCAGGCGCTGAGCGGTTGGATTGGGGCGGGCGG‑ACCGCAGGACTGGGCAACGCATATGCGGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAg                                                                                                                    <  2:196937/295‑1 (MQ=255)                                                                                                                                                                           aCCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAATCTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAgct                                                                                                                  <  1:519402/299‑1 (MQ=255)                                                                                                                                                                             cAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAgctgc                                                                                                                <  1:189973/299‑1 (MQ=255)                                                                                                                                                                              aaaCCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAgctgct                                                                                                               >  1:345852/1‑299 (MQ=255)                                                                                                                                                                                         tGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTg                                                                                                    <  1:440320/299‑1 (MQ=255)                                                                                                                                                                                                      gACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGaa                                                                                      >  1:245630/1‑299 (MQ=255)                                                                                                                                                                                                         cGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACAt                                                                                   >  1:421112/1‑299 (MQ=255)                                                                                                                                                                                                           tttCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACATCa                                                                                 <  1:403192/299‑1 (MQ=255)                                                                                                                                                                                                           tttCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACATCa                                                                                 <  1:281832/299‑1 (MQ=255)                                                                                                                                                                                                              gtgaGAAGGCCTTTGACGGAAGCTGATCGAGGTGGGTCCGAAAGCCCGGAGAGAGCCAGAAAGCGCCGGTGTGCGCTCGAAAGTCAGGTGAGGGGCGACGCAGGCGCGAAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACATCACTg                                                                              <  2:18776/296‑1 (MQ=255)                                                                                                                                                                                                                        aTTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGCACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCCCGAACGGACTGCGCTGCACGGTCTGGCTCACGCGCAAACCCTGCCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCCCCCCAAGCTACTGAAATATGCTTAACCGCG‑ATGGAAAAACACGGCAGGTTccag                                                                    >  2:590482/1‑297 (MQ=255)                                                                                                                                                                                                                             gCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACATCACTGAAGGTTCCGatgatg                                                               >  1:35070/1‑299 (MQ=255)                                                                                                                                                                                                                              cTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACATCACTGAAGGTTCCGAtgatga                                                              <  1:269025/299‑1 (MQ=255)                                                                                                                                                                                                                                  cGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCGGGGCCACGAACTGACTGCGATGCACGGGCTGGATCACGCGCAAACACTGGCTATCGCCCTGCCTGCACTGTGCAATGAAAACCGCGAGACCAAGCGCGCGAAGCTGCTGCCCTATGCTCAA‑CGCGTCTGGAACATCACTGCAGGATCCGCTGCTGAGCGt                                                          >  2:54081/1‑299 (MQ=255)                                                                                                                                                                                                                                    cTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTAt                                                        >  1:522030/1‑299 (MQ=255)                                                                                                                                                                                                                                    cTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTAt                                                        <  1:91471/299‑1 (MQ=255)                                                                                                                                                                                                                                     tGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGa                                                                                                                                                 >  2:38636/1‑210 (MQ=255)                                                                                                                                                                                                                                     tGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGa                                                                                                                                                 <  1:38636/210‑1 (MQ=255)                                                                                                                                                                                                                                     tGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGCTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCCCGCTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCCAGACCAAGCCCGCTAACCTGCTGCAATCTGCTGAA‑CCAGTCCGGCACATCCCAGACGGTTCCAAtcatgacactatt                                                       >  2:548492/1‑292 (MQ=255)                                                                                                                                                                                                                                          gAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGAcgc                                                  >  1:461140/1‑299 (MQ=255)                                                                                                                                                                                                                                                ggTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGAt                                            >  1:447681/1‑299 (MQ=255)                                                                                                                                                                                                                                                     gAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGGCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGCCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCg                                       <  1:590482/299‑1 (MQ=255)                                                                                                                                                                                                                                                                   agCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTc                         >  1:523635/1‑299 (MQ=255)                                                                                                                                                                                                                                                                       aGAAACCTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAATATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTg                     <  1:206383/299‑1 (MQ=255)                                                                                                                                                                                                                                                                         aaaaCTACGATGTGCGCGCCAACGTCATGTGGGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAg                   <  1:310294/299‑1 (MQ=255)                                                                                                                                                                                                                                                                         aaaaCTACGATGTGCGCGCCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAg                   >  1:144729/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                          gcCAACGTCATGTGAGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGAc  <  1:642282/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                                                               CAGGCGCGGTGATCTCCCGTAAAACCACAGGCGACAAGCAGGCGTTCCATTCTGCCCATGTTCAGCCGGTATTTGCCGTGCTCGATCCGGTTTATACCTACACCCTGCCGCCGCGTCAGGTGGCTAACGGCGTAGTGGACGCCTTTGTACACACCGTGGAACAGTATGTTACCAAACCGGTTGATGCCAAAATTCAGGACCGTTTCGCAGAAGGCATTTTGCTGACGCTGATCGAAGATGGTCCGAAAGCCCTGAAAGAGCCAGAAAACTACGATGTGCGCGCCAACGTCATGTGGGGGGCGACGCAGGCGCTGAACGGTTTGATTGGCGCTGGCGT‑ACCGCAGGACTGGGCAACGCATATGCTGGGCCACGAACTGACTGCGATGCACGGTCTGGATCACGCGCAAACACTGGCTATCGTCCTGCCTGCACTGTGGAATGAAAAACGCGAGACCAAGCGCGCTAAGCTGCTGCAATATGCTGAA‑CGCGTCTGGAACATCACTGAAGGTTCCGATGATGAGCGTATTGACGCCGCGATTGCCGCAACCCGCAATTTCTTTGAGCAATTAGGCGTGCCGAC  >  CP006584/3278256‑3278835

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑