Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	3,545,689	T→C	noncoding (2811/2931 nt)	LY180_16870 ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	3,545,689	0	T	C	100.0%	20.0 / NA	15	noncoding (2811/2931 nt)	LY180_16870	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (5/10);  total (5/10)

TCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATTTAGCTAC  >  CP006584/3545394‑3545772                                                                                                                                                                                                                                                                                                        |                                                                                    gctcGCGTGGGGGGACCCGTCACTGCCATCGGGGGTACGGCGTTTCACTTCTGAGGTGGGCTTGGGGTCAGGTGGGACCCCCGCGCTAAGGGCGCCAGGCAAAGTCTGTTTTATCAGACCGCTTTTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGAccctt                                                                                  <  2:265538/298‑2 (MQ=11)       aTGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTaaaggg                                                                            >  1:440556/1‑295 (MQ=14)                   cacaCTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGCTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTCAGGTTAAGCCTCAAGGTTCATTAGTACCGGTTAGCTCAACGCATCCCTCCGCCTACACACCCGGCCTATCACCGCCGTCGCCTTCAACGCTCTTTCAGAACCCCTAAAGGGTCCGGGAGAACt                                                                >  2:554989/1‑299 (MQ=11)                     cacttcctccGGGGCTTCGGCGGTTCACTTCTGGGTTCGGCGTGGGGTCAGGTGGGACCACCGCGGTAAGGCGGCCCGGCAAATGTTGTTTAATCATCCCGCTTCTGCGTTCTGATTTAATCTGTAGCGGGCTGAAAATCTTCTCTCAGCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTAGCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCa                                                              <  2:466412/290‑1 (MQ=11)                     cacTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCa                                                              >  1:622107/1‑299 (MQ=14)                            aTCGGCGCTACGGCGTTTCACTTCTGAGTTCGGAATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCggg                                                       <  1:68349/299‑1 (MQ=9)                                   ggagcggggttCACTTGTGAGTTCGGGAGGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCGGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCGGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGtt                                                <  2:70678/290‑1 (MQ=11)                                           tttCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCtt                                        <  1:51765/299‑1 (MQ=14)                                                cTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATg                                   <  1:368434/299‑1 (MQ=14)                                                         tCGGCTTGGGATCAGGTGGGGCCCCCGGGCTTAAGCCGCCGGGCATATTCTGTTTTATCAGCCCGCCGCTTCGTTCTGATTTAATTTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACGCCTTCGGCGTGGTAAGGGTAGGCCTCACGGTTCATTGGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCa                          <  2:579506/299‑1 (MQ=11)                                                                ggggTCAGGTGGGACGACCGCGCTAAGGCCGCCAGGCAATTTCTGTTTGTTCAGACCGCTTCTCCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCGCTCAGCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTAtct                   <  2:91413/299‑1 (MQ=11)                                                                ggggTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTAtct                   <  1:14623/299‑1 (MQ=14)                                                                   gTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCtt                <  1:637210/299‑1 (MQ=14)                                                                     cAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCACAGCATCGCTGCGCTTACACACCCGGCCTACCCAAGCCGTCGTCTTCAACGTTACCACACGCCCCTGAAAGGGCCGGGGAGAACCCTTCCCGGGCAACGGTTCGCCCTTCGTTGCTTTCAGCAACTAtctccctc              >  2:472460/1‑295 (MQ=11)                                                                           gagccaccGGGCTAAGGCCGCCAGTCAAATTTTGTTTTAGCCGCCCGCTGCTGCGTTCGTATTTAATCTGTATCAGGCTGAAAGTCTGCGCCCCGCCGCCAAGACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTCCCGGTTAGCTCAACGCATCGCTGCTCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCAttt        <  2:354735/296‑1 (MQ=11)                                                                                 ccGCGCTAAGGCCGCCAGGCAAATTATGTTTTATCATACCGCTTCTGCGTTCTGATTTAATCTGTATCCGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCACGCATCGCTGCTCTTACACACCCGGCCTATCAACGTCGCCGTCTTCAACGGTCCTTCAGGACCATTAAAGGGCCAGGGAGAACCCACCTCGGGGCAAGTTTCGTTCTTATATGCTTCAAGCACTTAACTCTTCCGCATTTAGCTAc  >  2:612291/1‑299 (MQ=11)                                                                                                                                                                                                                                                                                                        |                                                                                    TCTCGCATGGGGAGACCCCACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATTTAGCTAC  >  CP006584/3545394‑3545772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑