Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	3,545,695	G→A	noncoding (2805/2931 nt)	LY180_16870 ←	23S ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	3,545,695	0	G	A	100.0%	17.8 / NA	14	noncoding (2805/2931 nt)	LY180_16870	23S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/10);  total (4/10)

CACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATTTAGCTAC  >  CP006584/3545412‑3545772                                                                                                                                                                                                                                                                                            |                                                                              cacaCTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGCTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTCAGGTTAAGCCTCAAGGTTCATTAGTACCGGTTAGCTCAACGCATCCCTCCGCCTACACACCCGGCCTATCACCGCCGTCGCCTTCAACGCTCTTTCAGAACCCCTAAAGGGTCCGGGAGAACt                                                                >  2:554989/1‑299 (MQ=11)   cacttcctccGGGGCTTCGGCGGTTCACTTCTGGGTTCGGCGTGGGGTCAGGTGGGACCACCGCGGTAAGGCGGCCCGGCAAATGTTGTTTAATCATCCCGCTTCTGCGTTCTGATTTAATCTGTAGCGGGCTGAAAATCTTCTCTCAGCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTAGCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCa                                                              <  2:466412/290‑1 (MQ=11)   cacTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCa                                                              >  1:622107/1‑299 (MQ=14)          aTCGGCGCTACGGCGTTTCACTTCTGAGTTCGGAATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCggg                                                       <  1:68349/299‑1 (MQ=9)                 ggagcggggttCACTTGTGAGTTCGGGAGGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCGGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCGGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGtt                                                <  2:70678/290‑1 (MQ=11)                         tttCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCtt                                        <  1:51765/299‑1 (MQ=14)                              cTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATg                                   <  1:368434/299‑1 (MQ=14)                                       tCGGCTTGGGATCAGGTGGGGCCCCCGGGCTTAAGCCGCCGGGCATATTCTGTTTTATCAGCCCGCCGCTTCGTTCTGATTTAATTTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACGCCTTCGGCGTGGTAAGGGTAGGCCTCACGGTTCATTGGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCa                          <  2:579506/299‑1 (MQ=11)                                              ggggTCAGGTGGGACGACCGCGCTAAGGCCGCCAGGCAATTTCTGTTTGTTCAGACCGCTTCTCCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCGCTCAGCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTAtct                   <  2:91413/299‑1 (MQ=11)                                              ggggTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTAtct                   <  1:14623/299‑1 (MQ=14)                                                 gTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCtt                <  1:637210/299‑1 (MQ=14)                                                   cAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCACAGCATCGCTGCGCTTACACACCCGGCCTACCCAAGCCGTCGTCTTCAACGTTACCACACGCCCCTGAAAGGGCCGGGGAGAACCCTTCCCGGGCAACGGTTCGCCCTTCGTTGCTTTCAGCAACTAtctccctc              >  2:472460/1‑295 (MQ=11)                                                         gagccaccGGGCTAAGGCCGCCAGTCAAATTTTGTTTTAGCCGCCCGCTGCTGCGTTCGTATTTAATCTGTATCAGGCTGAAAGTCTGCGCCCCGCCGCCAAGACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTCCCGGTTAGCTCAACGCATCGCTGCTCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACCCTTAAAGGGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCAttt        <  2:354735/296‑1 (MQ=11)                                                               ccGCGCTAAGGCCGCCAGGCAAATTATGTTTTATCATACCGCTTCTGCGTTCTGATTTAATCTGTATCCGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCCACGCATCGCTGCTCTTACACACCCGGCCTATCAACGTCGCCGTCTTCAACGGTCCTTCAGGACCATTAAAGGGCCAGGGAGAACCCACCTCGGGGCAAGTTTCGTTCTTATATGCTTCAAGCACTTAACTCTTCCGCATTTAGCTAc  >  2:612291/1‑299 (MQ=11)                                                                                                                                                                                                                                                                                            |                                                                              CACACTACCATCGGCGCTACGGCGTTTCACTTCTGAGTTCGGCATGGGGTCAGGTGGGACCACCGCGCTAAGGCCGCCAGGCAAATTCTGTTTTATCAGACCGCTTCTGCGTTCTGATTTAATCTGTATCAGGCTGAAAATCTTCTCTCATCCGCCAAAACACCTTCGGCGTTGTAAGGTTAAGCCTCACGGTTCATTAGTACCGGTTAGCTCAACGCATCGCTGCGCTTACACACCCGGCCTATCAACGTCGTCGTCTTCAACGTTCCTTCAGGACTCTCAAGGAGTCAGGGAGAACTCATCTCGGGGCAAGTTTCGTGCTTAGATGCTTTCAGCACTTATCTCTTCCGCATTTAGCTAC  >  CP006584/3545412‑3545772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑