| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | CP006584 | 3,943,262 | G→A | G302D (GGC→GAC) | LY180_18795 → | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | CP006584 | 3,943,262 | 0 | G | A | 98.4% | 206.4 / NA | 64 | G302D (GGC→GAC) | LY180_18795 | bifunctional (p)ppGpp synthetase II/ guanosine‑3',5'‑bis pyrophosphate 3'‑pyrophosphohydrolase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (35/28); minor base C (1/0); total (36/28) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGCGCGGCAACCGTAAAGAGATGATCCAAAAAATCCTCTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑CGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTG > CP006584/3942970‑3943557 | gcgcgggGCAACCGGAAGTCGATTGTGCCAGAAATCTTGTCTGAAAGATAAGGGAGTTGGTAGGACGCGGGAATACCGGTCTGCGTGAGTGGTCGCGAAAGGCATCTTTTGTCGCTTTACTGCTAAATGGTGCGCAAAGAGCAGCGTTTTCACTTAAGCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCAAGATGCAC‑TGCCTTTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCa < 2:528436/299‑1 (MQ=255) cgcgcgGCAACCGTAAAGAGATGATCCAAAAAATCCTCTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAg < 1:221648/299‑1 (MQ=255) gcgcgGCAACCGTAAAGAGATGATCCAAAAAATCCTCTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGt < 1:433571/299‑1 (MQ=255) ggCAACCGTAAAGAGATGATCCAAAAAATCCTCTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCttt > 1:53848/1‑299 (MQ=255) cGTAAAGAGATGATCCAAAAAATCCTCTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCacac > 1:147531/1‑299 (MQ=255) gatgatCCAAAAAATCCTCTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCgatga < 2:53848/299‑1 (MQ=255) gatgatCCAAAAAATCCTCTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCgatga < 1:284850/299‑1 (MQ=255) aaaaaTCCTCTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGc > 1:631455/1‑299 (MQ=255) aaTCCTCTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCCTGCTGGGCCAAACTCAC‑AACCTGTCCAACCCGCGCCCAGGCCCCGTGAAAGACTATAACACCATTCCAAAAGCGATCGACCAAAAGCCACTGCACACACCCCTGATTGGcccgcccg > 2:132515/1‑299 (MQ=255) aTCCTCTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACgg < 1:147806/299‑1 (MQ=255) aaaTCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGtt > 1:82247/1‑299 (MQ=255) gTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGCTCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCCCGTGAAAGGCTATATCGCCATTCCAAAACCCACCAACCATCAGTCTTTGCACACCTCGACGATTGCCCCGCCCGGCGTGCCAGTTGCGGTcacaaac > 2:555413/1‑293 (MQ=255) gCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTa > 1:108901/1‑299 (MQ=255) aGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTAcc < 1:607694/299‑1 (MQ=255) aGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATCATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGACGCGTTAAAGCATATAGCGCCATTCCAAAAGCGAACGCCAACACGTCTTTGCCAACCTCGAGGATTGGCCCGCACGGGGTGCCGGTTGAGGCCCAGATCCGTAcc > 2:324789/1‑299 (MQ=255) aaGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTACGGGCCGCGTGAAAGACTATATCGCCATTCCAAAACCGAACGACTTTCAGTCTTTTCACACCTCGCACACTTGCCCGCCCGGCCGGCCGGCGGGGGTcccgccccgaacagaa > 2:356395/1‑285 (MQ=255) aCCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATc < 1:27045/299‑1 (MQ=255) cgcgTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGc < 1:647445/299‑1 (MQ=255) aaaGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCcg < 1:427225/299‑1 (MQ=255) aGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCcgcg > 1:329468/1‑299 (MQ=255) tCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACt > 1:420428/1‑299 (MQ=255) cTTTTTTCGATTTACTGGAAAATGGGGTTCAAAGAGAAGGGTTTTCCGTCAACCATGGACATCTAGCCTTTCCGCGTGACCGTCAATGAGTC‑TGACACCGGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTg < 2:112667/299‑1 (MQ=255) tATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGc < 1:556076/299‑1 (MQ=255) aTTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCt > 1:4676/1‑299 (MQ=255) gATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCCCACTGGGCTtata > 1:47461/1‑299 (MQ=255) tttACTGCAAAATGGTGCCCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGCGCCGCGGGAAAGCCTATAACGCCATTCCAAAAGCGAACGACTCTCAGGCTTTGCACACCCCGATGATTGGCCCCCACGGCGTGCCGGCTCGGGTCCAGATCCGTCCCGAAGATATGCAGCAGACGGCGGCGAGG‑GGTGTTGCCCCGCACTGGGCttcaaaa > 2:396771/1‑294 (MQ=255) tACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAaga > 1:536796/1‑299 (MQ=255) aaaaTGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCGTATAAAGAGCACgg > 1:526926/1‑299 (MQ=255) gTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAAc > 1:568427/1‑299 (MQ=255) tGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGAt > 1:170419/1‑256 (MQ=255) tGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGGGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGAt < 2:170419/256‑1 (MQ=255) aaGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGtact > 1:47246/1‑299 (MQ=255) aaGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGtact > 1:385338/1‑299 (MQ=255) cGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAACGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACCCCTCCATGATTGGCCCCCCCGGCCTGCCGTTTGGGGTCCAGACACGTACCGAAATTATGGAACCAAACGCGGCGTGGAGGTGTTG‑CGCCGAGTGGGCCTATCAATAGGACGGCGGAACCAggccagtcgcaca > 2:551719/1‑287 (MQ=255) cGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGcaca < 1:451784/299‑1 (MQ=255) cACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCcg < 1:132515/299‑1 (MQ=255) cTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCcgcg > 1:270697/1‑299 (MQ=255) tCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAg < 1:109289/299‑1 (MQ=255) ctgggcctCTGCGCGTTCCGTGTGTTCGTCAATGATTC‑TTCACCCTGTTATGGCGTGCTGGGCCAGATGCAC‑AGCCTGTCCAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCGTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAgcg < 2:329468/292‑1 (MQ=255) ggACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGCTGCAC‑AGCCTGTACACGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCACAAGCGAACGACTATCCTTCTTTGCACCCCCCGATGATTGGCCCGCACGGCCGCCCGGTTGCGGTCCACATCCGTACCGACCACATGGATCCGATGGCCGCGCTG‑GGCGCTGCCGCCCCCTTGGCTTCTACTGGGCCCCGCCAACCCCGTACCACCCCACACATCCGCCCCCacgcgc > 2:294891/1‑294 (MQ=255) ttCTACCCTGTCCGCGTGAACGTCAATGATACTTGAC‑CCTGTTCTGGCGGGCTGGCCCAG‑TGGACAAGCCTGTACAGCACGGGTCCGTGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACAACTCGATGATTGGCCCGCACGGCGGGCCGGTTGAGGTCCAGATCCGTACCGAAGGTATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGAt < 2:147531/298‑1 (MQ=255) gCTTTCCGCGTGATTGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGCTGCAAAg > 1:627004/1‑299 (MQ=255) tCAATGATTC‑TGCCACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGACCGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCCCGGCGTGGCGGTTGAGGCCCCGCCCCGTACCGAAGATATGGATCAGATGGCGGAGAGG‑GGTGGTGCCGCGCGATGGGGTTATAAAGAGGACGGGGGAACCGGTACTCCCGCACAAAACCCCGCACCGCGCCGGAACGACAGCCTGCCGGAGcgacac > 2:25295/1‑294 (MQ=255) gTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCg > 1:578447/1‑299 (MQ=255) ttATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGt < 1:589575/299‑1 (MQ=255) cgcgTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGGTGGCGGAGATG‑GGGGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCCAAACCAGTACCACCACACAAATCCACCCCCCTCGCCGGCTGCAAAGCCTGCTGGACGTGCAACAGCACGCCGGTCGTTCtttgta > 2:414657/1‑296 (MQ=255) gATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑Cg < 1:555413/299‑1 (MQ=255) aTGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑CGt < 1:25295/299‑1 (MQ=255) gCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑CGTTAAATcc > 1:103591/1‑299 (MQ=255) tGTACAAGCCGCGTCCGGCCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑CGTTAAATCCGAt < 1:15780/299‑1 (MQ=255) gTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑CGTTAAATCCGAtc < 1:232852/299‑1 (MQ=255) aaGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGACCGACTCTCAGTCTTTGCCCACCTCGATGATTGGCCCGCCCGGCGTGCCGGTTTAGGTCCAGATCCGTACCGAAGCTATTGTTCATATGTCGGAGATG‑GGTTTTGCCGCGCACTGGCCTTATACAGAGCCCGGCCACACCAGTACTACCGCACAAATCAGCGCCCAGCCCGTGACGCAACGCCTGCTGAAGCTGCCACAGCACGCCGGCACTTCGTTTGGATTTATCAAGAC‑CGTTAAATCCGaatcctt > 2:579029/1‑293 (MQ=255) ccGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCAAGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑CGTTAAATCCGATCTCTTccc < 1:315759/299‑1 (MQ=255) tCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑CGTTAAATCCGATCTCTTCCCGGATg < 1:423118/299‑1 (MQ=255) cggTGAAATACAATATCGCCATTCCAAAAGCGAACGACTATCAGGCTTTGCACACCGCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑CGTTAAATCCGATCTCTTCCCGGATGAGATTTAc < 2:578447/297‑1 (MQ=255) cgTGAAAGACTATATCGCCATTCCAAAAGCGTACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑CGTTAAATCCGATCTCTTCCCGGATGAGATTTACg > 1:184451/1‑299 (MQ=255) aaGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACCGGGCTTATAAAGAGCACGGCCAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGGCCCCCCACAGACCCCCGGTAGTTCGGTTGAATTT‑TCCAAAGCCGTCAAATCCCACCCCTTCCCGCATGCAAtttcacttttt > 2:47371/1‑291 (MQ=255) aGACTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGAACAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTAGTTTGAATTTATCGCGAG‑CGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTca > 1:366106/1‑299 (MQ=255) cTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑CGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCacac < 1:324789/299‑1 (MQ=255) cTATATCGCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑CGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCaaac > 1:377070/1‑299 (MQ=255) gCCATTCCAAAAGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑CGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAgg > 1:112264/1‑299 (MQ=255) aaaaGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑CGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTg > 1:209635/1‑299 (MQ=255) aaaaGCGAACGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTATTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑CGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTg > 1:581613/1‑299 (MQ=255) aCGACTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑CGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGctg < 1:356395/299‑1 (MQ=255) | GCGCGCGGCAACCGTAAAGAGATGATCCAAAAAATCCTCTCTGAAATCGAAGGGCGTTTGCAGGAAGCGGGAATACCGTGCCGCGTCAGTGGTCGCGAAAAGCATCTTTATTCGATTTACTGCAAAATGGTGCTCAAAGAGCAGCGTTTTCACTCAATCATGGACATCTACGCTTTCCGCGTGATCGTCAATGATTC‑TGACACCTGTTATCGCGTGCTGGGCCAGATGCAC‑AGCCTGTACAAGCCGCGTCCGGGCCGCGTGAAAGACTATATCGCCATTCCAAAAGCGAACGGCTATCAGTCTTTGCACACCTCGATGATTGGCCCGCACGGCGTGCCGGTTGAGGTCCAGATCCGTACCGAAGATATGGATCAGATGGCGGAGATG‑GGTGTTGCCGCGCACTGGGCTTATAAAGAGCACGGCGAAACCAGTACTACCGCACAAATCCGCGCCCAGCGCTGGATGCAAAGCCTGCTGGAGCTGCAACAGAGCGCCGGTAGTTCGTTTGAATTTATCGAGAG‑CGTTAAATCCGATCTCTTCCCGGATGAGATTTACGTTTTCACACCGGAAGGGCGCATTGTCGAGCTG > CP006584/3942970‑3943557 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |