Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	4,305,151	G→C	P51P (CCG→CCC)	LY180_20645 →	rhsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	4,305,151	0	G	C	96.7%	81.0 / NA	30	P51P (CCG→CCC)	LY180_20645	rhsA
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (13/16);  minor base A (1/0);  total (14/16)
Fisher's exact test for biased strand distribution p-value = 4.67e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

AAGCGCCGCACGGCGCTTTTTTTGTGCCTGTAATTTGCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGCCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGC  >  CP006584/4304857‑4305307                                                                                                                                                                                                                                                                                                       |                                                                                                                                                             aaGCGCCGCACGGCGCTTTTTTTGTGCCTGTAATTTGCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCATGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCcctgc                                                                                                                                                          <  1:442587/299‑1 (MQ=255)  aGCGCCGCACGGCGCTTTTTTTGTGCCTGTAATTTGCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCcctgcc                                                                                                                                                         >  1:69721/1‑298 (MQ=255)            ggcgacttttgtGGGCTTGTCATTTGCCTTTTTTAAATTCTTAAAAATTCCGGGGGGTTAATGCATTATCTGTTCTTGTTGTTGTCGGGCGCTAGGCTTGTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGTCGGCGCGTCAGGGCTACAGGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTCGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAg                                                                                                                                               <  2:315482/293‑1 (MQ=18)            ggCGCTTTTTTTGTGCCGTTAATTTTCGTTTTTTAAATTCTGAAGAATGCCGGGGGGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCGAGGCTTCTGGTTCGTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAg                                                                                                                                               <  2:315506/299‑1 (MQ=21)                 tttttttGTGCGTTGAATTTGCTTTTTATAATTTCTGAAAAAGTCTGGGGTTTTAACTCGTTATCATTTCCTGTTGTTGCCTGGCGCTAGGCTTCTGGTTCTTATTTAGTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGCCGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCt                                                                                                                                          <  2:614658/299‑1 (MQ=21)                   tttttGTGCCTGTAATTTGCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTc                                                                                                                                        <  1:210655/299‑1 (MQ=255)                         tgCCTGTAATTTGCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTg                                                                                                                                  <  1:233205/299‑1 (MQ=255)                                 aTTTGCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGTCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCTTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGTGTTCAGCCGGGGGCCGTATTGGTCCCCCACCCGGCGTGGCCTGTACCGTGCGCCCCGGCGGTGTGCCCGCCGACCACCCGGCGCACCCCCTGCCCGGTGCACGGGTCCTTCCCgcgtacacccac                                                                                                                          >  2:92422/1‑288 (MQ=21)                                     gCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCAGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCATTATGGCGGTAGCATTCTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCACCACCGGCGTGGCCTTTTCGGTTTGCCCCCGCGGCGTGACGCCCGGACCTCCGGTCAATCCACTGCTGTGTCCAAAGCTCCTTCCCGGTAAAACCGaacgcg                                                                                                                      >  2:427708/1‑294 (MQ=255)                                          gttatattgtttaaacaTTCCTGTGTGTTAACTTATTATCATTTCCTGTTGTTTACTGGCGTTATGCTTTTGGTTCTTATTTATTTCAAACGGTTTTTAAGATGGGCGGAAAACAGGCGGCGCGTCAGGGAGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGGCCCCACCGGGGTGGCTTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTg                                                                                                                 <  2:356073/287‑1 (MQ=17)                                           tataAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTgc                                                                                                                <  1:359199/299‑1 (MQ=255)                                                  tCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGcccggccc                                                                                                         <  1:311234/299‑1 (MQ=255)                                                   cTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTgcccggcccg                                                                                                        <  1:453546/299‑1 (MQ=255)                                                      aaaaaTTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGCTGCAAAGGTCCTTCCCGGTGACACCGACATCGCCCTGCCCGGCCCGCTg                                                                                                     >  1:425874/1‑299 (MQ=255)                                                         aaTTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCCTTGTCCACGGTTCAGCCGCGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGCTCGGGGTGCCCCGGCGCAGTTACGTCCGCCCCTCCGGCCAACCCCCCGCTCTGTGCAAAGCTCCTTCCCCGTGAAACCCACACCCCCCCGCCCGCCCCCCCGccc                                                                                                  >  2:557454/1‑298 (MQ=21)                                                              gggggTGTTAACTGTTTATCATTTCCTGTGTTTGACTGGCGTAAGGCTTCTGGTTGTTATTTATTTCAAAAGGATTTTAGGGTGGGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCAt                                                                                             <  2:122617/298‑1 (MQ=17)                                                                  gtgtTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGGGACGCCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGCCCCTCCCGGTGAAACCGACACCGCCCcccccgcccccccgcccgtcctccct                                                                                         >  2:408811/1‑289 (MQ=18)                                                                   tgtTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGATGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCctct                                                                                        >  1:96533/1‑299 (MQ=255)                                                                      tAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTccc                                                                                     <  1:239361/299‑1 (MQ=255)                                                                           cATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCAcc                                                                                <  1:390973/299‑1 (MQ=255)                                                                                     tCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGtt                                                                      >  1:132769/1‑299 (MQ=255)                                                                                         gttgttGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTAATACTCTCCCGCACCTAAAGCAGTTAcag                                                                  >  1:429005/1‑297 (MQ=255)                                                                                           tgttgACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTTAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGa                                                                >  1:144917/1‑299 (MQ=255)                                                                                               gagtGGCGCTGGGCTTCGGGTTCTTATTTATTTCGAAAGGATTTGAGGAGGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACaaa                                                            <  2:132769/296‑1 (MQ=14)                                                                                                      gcTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCACAGGTCCTTCCCGTTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACCGCAGTTACCGGACAAAAACGcac                                                     >  1:254719/1‑297 (MQ=255)                                                                                                                                cAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCgg                           >  1:213595/1‑299 (MQ=25)                                                                                                                                    aGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTgg                       <  1:171529/299‑1 (MQ=21)                                                                                                                                         tttACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAAt                  >  1:573241/1‑299 (MQ=17)                                                                                                                                              gATGGGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTg             <  1:74124/299‑1 (MQ=14)                                                                                                                                                    cGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGata       >  1:510524/1‑299 (MQ=14)                                                                                                                                                       aaaaCCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGTGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATcc    <  1:123058/299‑1 (MQ=14)                                                                                                                                                         aaCCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGAGTGACGTCCGGCCATCCGGTCAATCCCCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGGCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGc  >  1:172593/1‑299 (MQ=14)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                             AAGCGCCGCACGGCGCTTTTTTTGTGCCTGTAATTTGCCTTTTATAAATTCTGAAAAATTCCGGGGTGTTAACTCATTATCATTTCCTGTTGTTGACTGGCGCTAGGCTTCTGGTTCTTATTTATTTCAAAAGGATTTTACGATGAGCGGAAAACCGGCGGCGCGTCAGGGCGACATGACGCAGTATGGCGGTAGCATTGTCCAGGGTTCAGCCGGGGTACGTATTGGTGCCCCCACCGGCGTGGCCTGTTCGGTGTGCCCCGGCGGGGTGACGTCCGGCCATCCGGTCAATCCGCTGCTCGGTGCAAAGGTCCTTCCCGGTGAAACCGACATCGCCCTGCCCGCCCCGCTGCCGTTCATCCTCTCCCGCACCTACAGCAGTTACCGGACAAAAACGCCCGCGCCGGTGGGGAGCCTCGGCCCCGGCTGGAAAATGCCTGCGGATATCCGC  >  CP006584/4304857‑4305307

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑