Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	814,037	G→C	T139S (ACC→AGC)	gltA ←	type II citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	814,037	0	G	C	100.0%	175.8 / NA	52	T139S (ACC→AGC)	gltA	type II citrate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (28/24);  total (28/24)

AGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTG  >  CP006584/813748‑814330                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                        agagGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGATGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACcac                                                                                                                                                                                                                                                                                                >  1:484407/1‑299 (MQ=255)                         gTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGt                                                                                                                                                                                                                                                                        >  1:550478/1‑299 (MQ=255)                            cAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTcgc                                                                                                                                                                                                                                                                     >  1:538188/1‑299 (MQ=255)                                             ccATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAAc                                                                                                                                                                                                                                                    <  1:612853/299‑1 (MQ=255)                                                  gCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACg                                                                                                                                                                                                                                               <  1:583480/299‑1 (MQ=255)                                                      gTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCACGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTa                                                                                                                                                                                                                                           <  1:257543/299‑1 (MQ=255)                                                                   aTTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGGAATCTGCTCGTGGa                                                                                                                                                                                                                              >  1:579154/1‑299 (MQ=255)                                                                     tAACTTCATACGGTTCGCACGGATTGGAGAACATCATATTCAGGAAGTTACCGGCAGAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAAAACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATc                                                                                                                                                                                                                            <  1:360188/299‑1 (MQ=255)                                                                       aCTTCTTCCGGTTCGCCGGGGGTGGAAAAAATCATATTCAGGAAGTTACCGGCATAGGCGAGATCGGTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCAt                                                                                                                                                                                                                          <  2:507190/299‑1 (MQ=255)                                                                        cTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAGTCTGCTCGTGGATCATg                                                                                                                                                                                                                         >  1:323383/1‑299 (MQ=255)                                                                          gcgtgcGGTGCGGACGGCGTGGAGACCACAGTGTTCAGGAAGGTACGGGACTAGGTGAAATCGGTTGGCGGATAAACAAATGGCTGACCAATGGAAGACTTGTAACAGATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCTGCAATTTCACGATGACGAGGATTGTTAACAGCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGGCTGCCAGTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGt                                                                                                                                                                                                                       <  2:404745/294‑1 (MQ=255)                                                                            aTACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACAGGTAATCTGCTCGTGGATCATGGTAt                                                                                                                                                                                                                     >  1:322647/1‑299 (MQ=255)                                                                                   tCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGt                                                                                                                                                                                                              <  1:116379/299‑1 (MQ=255)                                                                                     gagaggaggggAGAACATTATATACAGGAAGTTACCGGCATAGGCGAGATCGGTGCGCGGGTAAACCAAAGGCGGACCAATGGAATTGTGTGAACACATCGCGGCCATGGTCGGCATGTTCGACAGCAGGCGGAGCGCGGCAATTTCACGATGACGAGGATTGTGAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACAGGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCa                                                                                                                                                                                                            <  2:583029/290‑1 (MQ=255)                                                                                      cACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCAc                                                                                                                                                                                                           >  1:221842/1‑299 (MQ=255)                                                                                         ggCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCCCGTTGATCATGGTAGGACGGGTCACCGt                                                                                                                                                                                                        >  2:174459/1‑299 (MQ=255)                                                                                               gagaACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGtttt                                                                                                                                                                                                  <  1:280291/299‑1 (MQ=255)                                                                                                agaACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAACCGTGGAACAGACGGGGAATACGCGCGTGGATCATGGTTTAGCGGGGCACCGGCGTTTTa                                                                                                                                                                                                 >  2:358351/1‑299 (MQ=255)                                                                                                agaACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCCCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGACACAGACGGGTAATCTGCTCGTGGACCATGGTATGACGGGGCCCCCTGGttttc                                                                                                                                                                                                 >  2:253273/1‑298 (MQ=255)                                                                                                   aCATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGCACGCCGCCAGCGCGCGCCTAATACCACCAATGACTGCACTAGGATGTGAGTCGCGACGGAAACCGGGGAACAGGTGGGTAAGTCGCACTGGGACCATGGTATCACGGGTACACGCAGTTTAAAAt                                                                                                                                                                                              >  2:636640/1‑299 (MQ=255)                                                                                                    catgatATTCGGGTTGTTACGGGCATAGGGGATATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACACCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCGAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAAtt                                                                                                                                                                                             <  2:484407/299‑1 (MQ=255)                                                                                                                  aGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTcc                                                                                                                                                                              <  1:358351/299‑1 (MQ=255)                                                                                                                               ggAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGttttt                                                                                                                                                                >  1:111877/1‑299 (MQ=255)                                                                                                                                      tCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCAtt                                                                                                                                                         >  1:78520/1‑299 (MQ=255)                                                                                                                                            cgcgGATAAACAAATGGCTGAACAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GGGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACCGCCATTGGGAGAGAGTCCAGACGGAAAGCGGTGAAAAGCCGGGGCAAAGGCCTGTGGATTATGGTCTGCAGGGTGCCACGAGGTTTAAATTCGTCAT‑TAGGTTTCTGAGTC‑GGttttaaccgcttgtgcag                                                                                                                                                   >  2:80096/1‑285 (MQ=255)                                                                                                                                                   aaaCAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGCTGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGCTGTGAGTCGCCACGGAAAGCGTGGAACAGACGGGAACTCGGCTCGTGGCACATGGTATGACGGGGCACCGTAGGTTTAACTTCATCATCACTG‑TCCTCAGTC‑AGTTTTTCACCCTTCCTCAGGCTGTaa                                                                                                                                            >  2:42269/1‑299 (MQ=255)                                                                                                                                                   aaaCAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTACCATCCAGCGAGTC‑GGGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGAATGCCATTGGATGTTAGTCGCGACGGAAAGCGTGGCACAGACGGGTAATCTCCTCGTGGATCATGTTATGGCGGGTCCACCAAGTTTTGAATTCGTAAT‑ACTGTTTCTTCGGG‑GGGTTTTCTCCCTTCAGCAggcggcaa                                                                                                                                            >  2:387854/1‑293 (MQ=255)                                                                                                                                                        aaTGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATCTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTTAGTCGCGACGGCAAGCCGGGAACAGACGGGTAAGCTGCCCCTGGATCAAGGTGTGCCTGTGCACGGGCGCTTTAAATCTGTCAG‑ACTGTTCCTG‑GTCGGGTTTTTCACTATTACATAGGATGGAACAAAc                                                                                                                                       >  2:82826/1‑299 (MQ=255)                                                                                                                                                                  cAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCCTGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAg                                                                                                                             >  2:132302/1‑299 (MQ=255)                                                                                                                                                                     tGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAATC‑GGCTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTa                                                                                                                          >  1:118133/1‑299 (MQ=255)                                                                                                                                                                                tAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGc                                                                                                               >  1:444690/1‑299 (MQ=255)                                                                                                                                                                                 aaCACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGcc                                                                                                              >  1:197702/1‑299 (MQ=255)                                                                                                                                                                                  acacATAGGGGCCATGGTGGGCGGTGGGGACAGCGGGCGGAACGCGGCAAGTTGGCGAGGACTAGGATTGTCAACATCCGGCGAGTC‑GTGAGAGAACGCCGCGAGCGGGCCGCTAATACCACACATGACTGCGAGTGGATGTGAGTCGCGACGGAAAGCATGGAACAGACGGGTAGTCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTGTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGcaa                                                                                                             <  2:562333/299‑3 (MQ=255)                                                                                                                                                                                           ggCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcga                                                                                                    <  1:304151/299‑1 (MQ=255)                                                                                                                                                                                            gCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcgat                                                                                                   >  1:248022/1‑299 (MQ=255)                                                                                                                                                                                             ccATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcgatc                                                                                                  <  1:335760/299‑1 (MQ=255)                                                                                                                                                                                               aTGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTa                                                                                                                          <  1:210191/273‑1 (MQ=255)                                                                                                                                                                                               aTGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGAAGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTa                                                                                                                          >  2:210191/1‑273 (MQ=255)                                                                                                                                                                                                      gCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAATC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGCATTTAGAATCGGTCGGCGGCTAACCGAACGGGAAACcg                                                                                         >  2:180425/1‑299 (MQ=255)                                                                                                                                                                                                      gCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTAACCGTAGTTTTAACTTCGTAAT‑ACTTTTCCTTAATC‑GGTTTTTCACCCTTCAGGAGGATCGAACAAACTTCCCGGAAGTTAGAATCGGTACGCAGAGGATGGAACGGGAAAccc                                                                                         >  2:501887/1‑298 (MQ=255)                                                                                                                                                                                                        aTTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACcgcg                                                                                       <  1:487161/299‑1 (MQ=255)                                                                                                                                                                                                             cGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGAGAGTC‑GTGACAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTTGATGTGAGTCGCGACGGAAAGCGTGGAACACACAGGTAATCTGCTCGTGGATCATGGTATGACGGGTCAACGTAGTTTTAAATTAGTCAT‑ACTTGTCCGAAGTC‑GGTTTTGCACCTATCAGCATGCTGTAACAAACTACCCGGAACTTAGAAAGCGTCTCCACCTGATCAATAGGGAAACCACGGTgac                                                                                  >  2:24697/1‑297 (MQ=255)                                                                                                                                                                                                              gACGGCAGGCGGAACGCTGAGATTGCACGAGGCCTGTGAAGTTTAACATCCAGCGAGTC‑GTGATAAAACGCCGCCAGCGCGCCGCTAATACCACACAGGCCTGCCAGTGGATGTGAGTCGAGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTgcag                                                                                 <  2:550478/299‑1 (MQ=255)                                                                                                                                                                                                                          aaCGCGGCAATTCAATGCAGACGAGGATAGTTAACAGCCAGGCAGTCTGTG‑GAGAACGCCGCCAGCGCGCCGCTAACACCACACATGACGGCCATGGGGTGTGAGTCGCGACGGAAAGCGGGGAACAGACGGGTAAGCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCttg                                                                     <  2:30443/299‑2 (MQ=255)                                                                                                                                                                                                                                      tCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAAt                                                         <  1:387854/299‑1 (MQ=255)                                                                                                                                                                                                                                                   ggttGTTAACATCCAGCAGGTC‑GTGATAGAACCCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGGCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTa                                            <  2:78520/297‑1 (MQ=255)                                                                                                                                                                                                                                                   gATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTa                                            <  1:35412/299‑1 (MQ=255)                                                                                                                                                                                                                                                            cATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAg                                   <  1:583689/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                  aCGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCt              <  1:224549/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                      cgccAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGt          >  1:44874/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                          aGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGGGCAGCAAAATACCTTCATCCCCATCAATAAAAGTAATTTTAGATTCGCAGGATACGGTTGAAGTGAAGACAGGGGCaaa      >  2:450568/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                             gcgcCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGt   <  1:82826/299‑1 (MQ=255)                                                                                                                                                                                                                                                                                              cgccgcTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTg  >  1:194588/1‑299 (MQ=255)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                        AGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTC‑GTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATGACGGGTCACCGTAGTTTTAAATTCGTCAT‑ACTGTTCCTGAGTC‑GGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAAAGGTG  >  CP006584/813748‑814330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑