breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 30 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0065841,563,2370TC72.5% 29.9 / 24.5 41V173A (GTT→GCT) LY180_07445transposase IS609
*CP0065841,643,5820GA61.3% 93.5 / 125.5 127intergenic (‑242/‑61)LY180_07760/LY180_07765sulfatase/transposase
*CP0065843,513,9650TC48.9% 0.8 / 63.4 47intergenic (‑141/+198)tldD/LY180_16725protease TldD/membrane protein
*CP006584269,1160CT45.2% 24.5 / 102.3 87R121R (CGC→CGTLY180_01180hypothetical protein
*CP006584269,4380TC36.8% 92.9 / 112.1 118L55P (CTC→CCC) LY180_01185hypothetical protein
*CP0065844,253,9020CT36.1% 134.5 / 112.3 124intergenic (+24/‑40)LY180_20335/LY180_20340hypothetical protein/hypothetical protein
*CP006584269,4000GA36.0% 107.9 / 98.9 112E42E (GAG→GAALY180_01185hypothetical protein
*CP0065841,310,8320TG35.7% 63.2 / 76.7 70intergenic (‑288/+141)LY180_06180/LY180_06185hypothetical protein/hypothetical protein
*CP006584269,4430TG35.5% 85.2 / 142.4 121F57V (TTT→GTT) LY180_01185hypothetical protein
*CP006584269,4520CT35.5% 120.8 / 112.4 122Q60* (CAA→TAA) LY180_01185hypothetical protein
*CP006584269,4530AG35.2% 105.4 / 121.8 122Q60R (CAA→CGA) LY180_01185hypothetical protein
*CP006584269,4030TC33.9% 103.5 / 105.3 113G43G (GGT→GGCLY180_01185hypothetical protein
*CP0065841,310,8330AG33.8% 70.2 / 60.5 69intergenic (‑289/+140)LY180_06180/LY180_06185hypothetical protein/hypothetical protein
*CP0065844,253,9760TA33.1% 151.3 / 135.0 133F12Y (TTC→TAC) LY180_20340hypothetical protein
*CP006584269,4920CT32.2% 133.0 / 93.7 121T73I (ACC→ATC) LY180_01185hypothetical protein
*CP006584269,0410TC32.1% 88.9 / 65.4 84D96D (GAT→GACLY180_01180hypothetical protein
*CP006584269,0440TC31.3% 88.6 / 65.3 84C97C (TGT→TGCLY180_01180hypothetical protein
*CP0065841,643,6180CG30.5% 186.7 / 123.6 132intergenic (‑278/‑25)LY180_07760/LY180_07765sulfatase/transposase
*CP0065844,253,8790GA30.5% 176.6 / 90.7 119intergenic (+1/‑63)LY180_20335/LY180_20340hypothetical protein/hypothetical protein
*CP0065844,254,0250GA29.5% 184.7 / 93.4 132A28A (GCG→GCALY180_20340hypothetical protein

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP006584 2253821 =30 (0.490)14 (0.240) 12/558 3.4 34.8% coding (38/414 nt) LY180_10760 hypothetical protein
?CP006584 2255662 = 24 (0.410)coding (826/1284 nt) LY180_10775 tail protein
* ? CP006584 4739807 =67 (1.100)8 (0.140) 8/570 4.4 10.3% intergenic (+48/‑432) LY180_22625/fimA tyrosine recombinase/type‑1 fimbrial protein subunit A
?CP006584 4740121 = 74 (1.250)intergenic (+362/‑118) LY180_22625/fimA tyrosine recombinase/type‑1 fimbrial protein subunit A
* ? CP006584 = 12134840 (0.000)8 (0.130) 8/588 4.6 100% intergenic (+183/‑112) LY180_05705/LY180_05710 DNAse/PTS glucose transporter subunit IIBC
?CP006584 1213492 = 0 (0.000)intergenic (+191/‑104) LY180_05705/LY180_05710 DNAse/PTS glucose transporter subunit IIBC
* ? CP006584 = 3884875120 (1.960)6 (0.100) 6/586 5.2 5.0% coding (905/1137 nt) LY180_18515 membrane protein
?CP006584 3885089 = 108 (1.770)coding (691/1137 nt) LY180_18515 membrane protein
* ? CP006584 794676 =NA (NA)7 (0.120) 6/578 5.2 NA coding (1871/4200 nt) LY180_03720 rhsA
?CP006584 = 794771 NA (NA)coding (1966/4200 nt) LY180_03720 rhsA
* ? CP006584 = 4400614NA (NA)4 (0.070) 3/586 6.5 5.0% intergenic (+871/‑175) LY180_21040/LY180_21045 hypothetical protein/5S ribosomal RNA
?CP006584 = 4416210 77 (1.260)coding (663/690 nt) LY180_21095 (alpha)‑aspartyl dipeptidase
* ? CP006584 2786005 =53 (0.870)3 (0.050) 3/588 6.5 4.0% coding (449/1623 nt) LY180_13205 L‑aspartate oxidase
?CP006584 = 3928372 92 (1.510)intergenic (‑290/‑123) LY180_18705/LY180_18710 lipopolysaccharide core heptosyltransferase RfaQ/3‑deoxy‑D‑manno‑octulosonic acid transferase
* ? CP006584 = 49793459 (0.970)3 (0.050) 3/578 6.5 4.8% coding (719/1848 nt) secD preprotein translocase subunit SecD
?CP006584 = 497954 60 (1.000)coding (739/1848 nt) secD preprotein translocase subunit SecD