Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	1,505,880	T→C	L35L (CTA→CTG)	LY180_07200 ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	1,505,880	0	T	C	98.1%	186.3 / ‑6.2	53	L35L (CTA→CTG)	LY180_07200	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (25/27);  minor base A (1/0);  total (26/27)
Fisher's exact test for biased strand distribution p-value = 4.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01

CATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAATAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGAATTACGTCGTTGGATTTATCTTCAAAGAATAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGATTTTTCAGCGCTTCAATTGGCGTAACG  >  CP006584/1505592‑1506033                                                                                                                                                                                                                                                                                                 |                                                                                                                                                          cATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGCGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAAATGCACGttc                                                                                                                                                  >  2:247549/1‑297 (MQ=255)   ttagttccACCTCCCTACATCAGCAGGCTTACGGCAACGAGCGCGGCTACTTTCTTCATGGTGGTGTTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTGGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCGGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTtgtg                                                                                                                                                <  2:613336/298‑1 (MQ=255)          cacaTCCCGCCATCAGCAGGCTTAGCGCAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTTCAGCGTAGGCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTggcaggca                                                                                                                                         >  2:181128/1‑298 (MQ=255)           acaTCCCGCCATCAGCAGGCTTAGCGCAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGgcaggcag                                                                                                                                        >  1:84623/1‑298 (MQ=255)           acaTCCCGCCAGCAGCAGGCTTATCGCAACGGGCGCGGGTACTTTCTTCATTGTGGTTTTCAAGTACAGTTTCTGACTCAGGGCTAGTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGgcaggcag                                                                                                                                        <  2:381642/298‑1 (MQ=255)             aTCCCGCCATCAGCAGGCTTAGCGCAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGAc                                                                                                                                      <  1:501517/298‑1 (MQ=255)              tCCCGCCATCAGCAGGCTTAGCGCAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACa                                                                                                                                     >  1:825234/1‑298 (MQ=255)                 cGCCATCAGCAGGTTTAGCGCAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGc                                                                                                                                  <  1:660034/298‑1 (MQ=255)                  gCCATCAGCAGGCTTAGCGCAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCTCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCTGCGTAGTCTGAGACATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCg                                                                                                                                 >  1:940987/1‑298 (MQ=255)                      tCAGCAGGCTTAGCGCAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGCAATGCCTGTTGCCCGGTAAACAGCACGTTGTGGCAGGCAGCCGGGCGACgg                                                                                                                             >  2:979067/1‑298 (MQ=255)                              cTTAGCGCAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTc                                                                                                                     <  1:524439/298‑1 (MQ=255)                               ttAGCGCAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCGGGGAAACAGCACGTGTTGGCAGGCAGACAGGCGACGGAAAACGTCa                                                                                                                    >  2:250021/1‑298 (MQ=255)                               ttAGCGCAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCa                                                                                                                    >  1:259010/1‑298 (MQ=255)                                    tcAACGTGCGGGGCTATTTTCGTTATTGTGGTTCTGCGTTATAGTCTCAGACTGAGGGCTATTGTAAAGATAGAGGAGGAAAGGTCATGGGGGCTTATCTGAAGCAGGTCCACTGGGTTGCAGGGCAGCGGCAAGATTAAACGAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTTCTTGAGTTTGGCAGCGTAGTCTGAGAAATACTGGTGAGAGCTTGTGCTGTCAGGAAAGCCTGGTGCCCGGTAAACAGCACGTGGTGGCAGGCAGACAGGCGACGTAATACGTCATCCTg                                                                                                               <  2:778979/297‑1 (MQ=255)                                            cgcgGCTACTTTCTTGATTGGTGTTCTCAATTACAGTTTCTGACTAAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAGGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGGGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCAcgt                                                                                                       <  2:967888/298‑1 (MQ=255)                                                gCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGtt                                                                                                   <  1:85291/298‑1 (MQ=255)                                                        cttcATTATGGTTCTCAATTACAGTTTCTGACTCAGTGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTAt                                                                                           <  1:747694/298‑1 (MQ=255)                                                         ttcATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATc                                                                                          <  1:559910/298‑1 (MQ=255)                                                                tgGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAg                                                                                   >  1:835942/1‑298 (MQ=255)                                                                  gTTCTCAATTACTGTTTCTTACTCAGGTCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCGCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGaa                                                                                 <  2:215319/298‑1 (MQ=255)                                                                   ttCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGaac                                                                                >  1:589754/1‑297 (MQ=255)                                                                     ctcAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAg                                                                              >  1:681339/1‑298 (MQ=255)                                                                                tttCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCTGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGCACAGATCGcgttcgt                                                                   >  1:931473/1‑298 (MQ=255)                                                                                 ttCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGcgttcgtt                                                                  <  1:95104/298‑1 (MQ=255)                                                                                   gtggatccgggCTATTTTAAGAATAGAGGATGAAAGGTCATTTGGGATTGTCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTGTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTtct                                                                <  2:404779/290‑1 (MQ=255)                                                                                     gACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCa                                                              <  1:968074/298‑1 (MQ=255)                                                                                      aCTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGCTTGCTTAAGCTTTGCAGCGTAGTCTGAGACATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTTAACAGGACGTTGTGGCAGGAAGACCGGCCGCGGACTAACTCACCCTGGATCAGGTGGTCTGCGTTATCTTCCAAGAACAGATCGCGTTCGTtctcta                                                             >  2:222195/1‑296 (MQ=255)                                                                                       cTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATa                                                            <  1:601452/298‑1 (MQ=255)                                                                                           gggCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTGCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGAAGGCAGACAGGCTACGGAATACGTCATCCCGGATCACGTCGTTGGATTTATCTTAAAAGAACAGATCGCGTTCGGTCCCAAACCCg                                                        >  2:875466/1‑298 (MQ=255)                                                                                               aaTTTTAAGAATTGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGTTCCCCTGGGTTGCAGGGGGGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCa                                                    <  2:488027/297‑1 (MQ=255)                                                                                                   ttAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATAcc                                                >  1:868832/1‑298 (MQ=255)                                                                                                         aTAGAGGATGAAAGTGTGGTGGGGATTATCTGTATCAGCTCCCCTGGGTTGCAGGGTGGCGGCAAGATGAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTTCTTAAGTTTTGCAGCGTAGGCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGa                                          <  2:485505/298‑1 (MQ=255)                                                                                                         aTAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGTTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATAAGTCATCCTGGATCCCGACGTTGGATTTATCGTCAAAGAACAGATCGCCTTCGTTCTCATCCACGCCCATACCCaccaa                                          >  2:845667/1‑294 (MQ=255)                                                                                                         aTAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGa                                          >  1:907469/1‑298 (MQ=255)                                                                                                            tgtgagtaaaGGTCTTGGGGGGTTATCGTGTTGAGCCCCCCTGGGTTGCAGGGGTGCGGCAAGATTAAACCAGTTCGTGCGGGCAGGTTTAGACTGTGGCCGGTATGCTTAAGTTTTGCAGCGTAGTCGGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACATCACGTTATGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACTGATCGCGTTCGTGCTCATACACGTCCATACCCAACGAAcc                                       <  2:804522/291‑1 (MQ=255)                                                                                                            gagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAAcc                                       <  1:392988/298‑1 (MQ=255)                                                                                                            gagGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAAcc                                       >  1:915942/1‑298 (MQ=255)                                                                                                              ggATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGCCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGGTTTATCTTCAAAGAACAGACCGCGTTCGTTCGTATACCCGTCCACACCCCACGAACCaa                                     >  2:166036/1‑298 (MQ=255)                                                                                                                aTGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAAtt                                   <  1:99414/298‑1 (MQ=255)                                                                                                                   aaaGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCCCATTCAAGTCCATAACCAAAGAACCAATTTTc                                >  2:234730/1‑298 (MQ=255)                                                                                                                    aaGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCt                               <  2:42437/298‑1 (MQ=255)                                                                                                                       gTCATTGGGGATTATCTGAATCAGCTCCCCTGGGTTGCAGGTGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAAGACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTGGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATGTTCTGAt                            <  1:155922/298‑1 (MQ=255)                                                                                                                              gggATTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATCCGTCATCCTGGATCACGTCGTTGGAGTGATCTTCAACGAAAAGATCGCGTTCGTGCTCTCACACGTCCATACCCAAAGAACCAATTTTCTGCTTTTTCat                     >  2:481358/1‑297 (MQ=255)                                                                                                                                 aTTATCTGAATCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGATTTTTCAgcgc                  <  1:340489/298‑1 (MQ=255)                                                                                                                                          aTCAGCTCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGAAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCCAAGCACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACAAACCAATTGTCTGATTTTTAACCGATTCAAtttc         >  2:188066/1‑296 (MQ=255)                                                                                                                                                tCCCCTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGATTTTTCAGCGCTTCAATTgctgcctg   <  1:817334/298‑8 (MQ=255)                                                                                                                                                 ccccTGGGTTGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGATTTTTCAGCGCTTCAATTgctgcctga  >  1:135184/1‑290 (MQ=255)                                                                                                                                                                                                                   aTTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCaaa                                                                                    >  2:377223/1‑150 (MQ=255)                                                                                                                                                                                                                   aTTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCaaa                                                                                    <  1:377223/150‑1 (MQ=255)                                                                                                                                                                                                                      gCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGa                                          <  2:678080/189‑1 (MQ=255)                                                                                                                                                                                                                      gCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGa                                          >  1:678080/1‑189 (MQ=255)                                                                                                                                                                                                                                                       ggTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAAt                                    >  2:414151/1‑162 (MQ=255)                                                                                                                                                                                                                                                       ggTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAACAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGGATCACGTCGTTGGATTTATCTTCAAAGAACAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAAt                                    <  1:414151/162‑1 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                          CATTACTTACACATCCCGCCATCAGCAGGCTTAGCGCAACGAGCGCGGCTACTTTCTTCATTGTGGTTCTCAATTACAGTTTCTGACTCAGGGCTATTTTAAGAATAGAGGATGAAAGGTCATTGGGGATTATCTGAATCAGCTCCCCTGGAATGCAGGGGAGCGGCAAGATTAAACCAGTTCGTTCGGGCAGGTTTCGCCTTTTTCCAGATTGCTTAAGTTTTGCAGCGTAGTCTGAGAAATACTGGTCAGAGCTTCTGCTGTCAGGAATGCCTGGTGCCCGGTAAATAGCACGTTGTGGCAGGCAGACAGGCGACGGAATACGTCATCCTGAATTACGTCGTTGGATTTATCTTCAAAGAATAGATCGCGTTCGTTCTCATACACGTCCATACCCAACGAACCAATTTTCTGATTTTTCAGCGCTTCAATTGGCGTAACG  >  CP006584/1505592‑1506033

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑