Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	2,265,885	C→A	A155A (GCC→GCA)	LY180_10860 →	terminase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	2,265,885	0	C	A	83.7%	65.2 / 13.1	43	A155A (GCC→GCA)	LY180_10860	terminase
Reads supporting (aligned to +/- strand):  ref base C (0/7);  new base A (19/17);  total (19/24)
Fisher's exact test for biased strand distribution p-value = 1.23e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.65e-01

TCGAAATGAGTCTGGAAGCGCGGCTGACCCAGCTCATCATCAAACCGCAGAAAACCGGCGGTGACTTCAAGGAAATTGACCTGCTCGGAC‑GCCAGATTGAACGACTGGCACGGGTCAACCGTTACAGTCAGACCGGCAACGAGGCAGACCTTAATCCGAACGTCGCTAACCGCAACAAAGGCGGGCGTCGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCCGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAATTGTCCTGGGCAATAACGGCGCAAAACTGATTTTTCTCGGCACCAACTCCAACACCGCGCAGAGCCATAACGGCGACCTGTACGTC  >  CP006584/2265596‑2266176                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                     tccaaaTGAGTCAGGAGGGCCGCCGGGCCCAGCACAGCATGAAACCGCAGCAAACCGGCGGGGACTGCAAGGAAATGGGCCGGCTCGGAC‑GCCAGATGGGAGGCCGGGCACGGGTCAACCGGTGCAGTCAGACCGGCAACGAGGCAGACCTTAATCCGAACGTCGCTAACCGCAACAAAGGCGGGCGTCGCAAACCGGAAAAGAATTTTTTCAGTGACGAGGCCATCGAAGAGCTGGAGCAGATTTTCGTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCCGGGCTTGa                                                                                                                                                                                                                                                                                              <  2:370518/295‑1 (MQ=1)                             cccgCTCAGCATCAAACCGAAGAAAACCGGCGGTGAGTTCAAGGAAATGGACCTGCTCGGAC‑GCCAGATTGAACGACTGGAACGGGTCAACCGTGACAGTCAGACCGGCAACGAGGCAGACCTTAATCCGAACGTCGCTAACCGCAACAAAGGCGGGCGTCGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATcc                                                                                                                                                                                                                                                                  <  2:143602/295‑1 (MQ=0)                                     tgcagaacacggaGAAACCAGGCGGTGGCTGCAAGGAGAGTTACCGGCTCGGAC‑GCCAGGTTGACGGACTGGCACGGGTCCACCGTTACAGTCAGACCGGCAACGAGGCAGACCTTAAGCCGAGCGTCGCTAACCGCAACAAAGGCGGGCGTCGCAAACCGAGAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCCGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGAt                                                                                                                                                                                                                                                          <  2:734637/286‑1 (MQ=1)                                                                acggcagggAAATGGACCTG‑TCCGACGGCCAGATGAAACGACGGGCAAGGTGAAACCGTTACCGGCAGACCGGCAACGAGGCAGACCTTAATCCGAACGTCGCTAACCGAAACAAAGGCGGGCGTCGCAAACCGAAAAAGAATTGTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCCGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGa                                                                                                                                                                                                                               <  2:192659/291‑1 (MQ=1)                                                                                ccggcccGGAC‑GCTGGATTGAACGACTGGCCCGGGCAAACCGTTCCAGTCAGCCCGGCAACGCGGCAGACCTTAATCCGAACGTCGCTAACCGCAACAAAGGGGGGCGTCGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCCGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGcc                                                                                                                                                                                                               <  1:519612/292‑1 (MQ=1)                                                                                  ctcagtggatgacAGAATTAACGACGTACACGGGGAAACCGTTAGAGTCAGACCCGAGACGACGCAAACCTGAATCCGAACGTCGCCACACGAAACAAAGGCGGGCGTCGCAAACCGAAAATAAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGAAGATTTTCGTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCGTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGccc                                                                                                                                                                                                              <  2:534474/286‑1 (MQ=1)                                                                                        agggaccggATTGCACGAGTGGGACGGGTAAACCGTTCCAGGCAGACCGGCGACGCGGGAGACCGTAACCCGAACGTGGCTAGACGGAACTAGGGCGGGGGGAGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATAGCAGTTGCACTGGTATCGCGCCGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCGGCGCGCCCTGaaaa                                                                                                                                                                                                        <  2:1004914/290‑1 (MQ=1)                                                                                                                     cacccGTGACAGTCAGACCGGCACAGAGGCAGACGTAATTCCAGACGTCGCTAACCGCAACACAGGAGGGCGTCGCAAACCGAAAAAGACTTTTTGCAGTGACGAGGCCATCGACAAGCTGTAGCAGATTTTCTTTGAGCAGTCTGTCGAATCGCAGTTGCGCTGGTATCGCGCCGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATGGGCGCGACGTTTGATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGcc                                                                                                                                                                           <  2:987158/295‑1 (MQ=1)                                                                                                                      aaacggtACAGACAGACCGGCAACGGGGCAGACCTGAATCCGAACGTCGTTAACCGCAACAAAGGCGGGCGTCGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCCGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCa                                                                                                                                                                          <  2:712291/292‑1 (MQ=0)                                                                                                                                                     aCCTTAATCCGAACGTCGCTAACCGCAACAAAGGCGGGCGTCGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATa                                                                                                                                           <  1:833191/298‑1 (MQ=14)                                                                                                                                                        ttAATCCGAACGTCGCTAACCGCAACAAAGGCGGGCGTCGCAAACCGAAAAAGAAGTTTTGCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATAcat                                                                                                                                        <  1:614326/298‑1 (MQ=14)                                                                                                                                                               gAACGTCGCTAACCGCAACAAAGGCGGGCGTCGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGTAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGGTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGCCATAACCAGATTTTTCTTCTGGCCATTAAAACGCACGCCGATGTTTTACGCCAATAccttctcccc                                                                                                                                 >  2:914607/1‑289 (MQ=11)                                                                                                                                                                 aCGTCGCTAACCGCAACAAAGGCGGGCGTCGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCtt                                                                                                                               >  1:227955/1‑298 (MQ=14)                                                                                                                                                                     cGCTAACCGCAACAAAGGCGGGCGTCACAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTTAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGcc                                                                                                                           >  1:812890/1‑298 (MQ=14)                                                                                                                                                                         aaCCACAACAAAGGCGGGCGTCGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑c                                                                                                                      <  1:294629/298‑1 (MQ=14)                                                                                                                                                                                       cgggcgTCGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCCACCCCTGGAAACCGGTCATAACCAGATTTTTCTTTCGGCCAGGAAACCGCAGGGGAATGTTTTCCGCGAATACATCATCCCCTTCACCCGG‑CGGgttggcgttgac                                                                                                        >  2:282081/1‑298 (MQ=11)                                                                                                                                                                                         ggcgTCGCCAAGCGGAAAAGAATTGTTTCAGTGACGAGGCAATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTGTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTGTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTtgacct                                                                                                      <  2:382780/298‑1 (MQ=9)                                                                                                                                                                                         ggcgTCGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTtgacct                                                                                                      <  1:488540/298‑1 (MQ=14)                                                                                                                                                                                         ggcgTCGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTtgacct                                                                                                      >  2:418374/1‑298 (MQ=14)                                                                                                                                                                                         ctcgTCGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTtgacct                                                                                                      <  1:519386/296‑1 (MQ=14)                                                                                                                                                                                          gcgTCGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGAAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGCAAACCGGTCATAACCAGCTTTTTCTGTCGGCCAGTAAAACGCAGGCGTCTGTGTTCCGCGAATCTCTCACTCCCTTTGCCCGGCCTGtttgccgttaactg                                                                                                      >  2:429990/1‑296 (MQ=11)                                                                                                                                                                                           cgTCGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTtgacctga                                                                                                    <  1:515138/298‑1 (MQ=14)                                                                                                                                                                                            gTCGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTTTTGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCCTCGCCTTTGCCCGG‑CTGGGTGACGTtggcctgtg                                                                                                   >  2:519386/1‑296 (MQ=9)                                                                                                                                                                                                         aaaGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAAtt                                                                                      >  1:958379/1‑298 (MQ=17)                                                                                                                                                                                                          aaGAATTGTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAATTg                                                                                     <  1:92185/298‑1 (MQ=17)                                                                                                                                                                                                            gAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAATTGTc                                                                                   <  1:705529/298‑1 (MQ=17)                                                                                                                                                                                                                        ggagggggCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAATTGTCCTGGGCAATAAc                                                                       <  2:227955/292‑1 (MQ=17)                                                                                                                                                                                                                          aCGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCCCCTTTGCCCGG‑CTGGTTGACGTTGACCCGCCAGGTGCCCCACTTGTCCTGGGCAAAAAggg                                                                     >  2:117180/1‑298 (MQ=255)                                                                                                                                                                                                                           cGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAATTGTCCTGGGCAATAACGgc                                                                    >  1:917824/1‑298 (MQ=17)                                                                                                                                                                                                                             aGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCCGTAAAACGCAGGCGTATGTGTTCCGCGAATACATAATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTCGCCCACTTTTCCTGGGCAATAACggacc                                                                  >  2:169971/1‑295 (MQ=255)                                                                                                                                                                                                                                ccATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAATTGTCCTGGGCAATAACGGCGCaaa                                                               >  1:588545/1‑298 (MQ=17)                                                                                                                                                                                                                                 cagcGAAAGGCGGGAGCGGTTTTTCTTTGGGCAGTCTTTCGAATGTCATTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCAGCCGCGATATCCTGAAAGCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAATTGTCCTGGGCAATAACGGCGCaaaa                                                              <  2:741535/295‑1 (MQ=11)                                                                                                                                                                                                                                           gtggcggagtgtttCTTTGAGGAGTCTTTCGAATTATAGTTGCACTGGTCTCGCGCAGGGCGTGGGCACCGCATCCGGGATATCCTTAAATCCCGCCAGATTGGCGGGACGTTTGATTTTGCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAACCGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACTCAATTGTCCTGGGCAATAACGGCGCAAAACTGATTTTtc                                                    <  2:812890/287‑1 (MQ=11)                                                                                                                                                                                                                                            tGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGTGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAATTGTCCTGGGCAATAACGGCGCAAAACTGATTTTtct                                                   >  1:699666/1‑298 (MQ=17)                                                                                                                                                                                                                                                gCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAATTGTCCTGGGCAATAACGGCGCAAAACTGATTTTTCTCGGc                                               >  1:204512/1‑298 (MQ=17)                                                                                                                                                                                                                                                               gCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAATTGTCCTGGGCAATAACGGCGCAAAACTGATTTTTCTCGGCACCAACTCCAACAcc                                >  1:278645/1‑298 (MQ=17)                                                                                                                                                                                                                                                                  gTCTTTCGAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAATTGTCCTGGGCAATAACGGCGCAAAACTGATTTTTCTCGGCACCAACT‑CAACACCgcgc                            >  1:417364/1‑298 (MQ=17)                                                                                                                                                                                                                                                                         gAATATCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAATTGTCCTGGGCAATAACGGCGCAAAACTGATTTTTCTCGGCACCAACTCCAACACCGCGCAGAGcc                      <  1:915389/298‑1 (MQ=18)                                                                                                                                                                                                                                                                           atatCAGTTGCACTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAATTGTCCTGGGCAATAACGGCGCAAAACTGATTTTTCTCGGCACCAACTCCAACACCGCGCAGAGCCAt                    >  1:600726/1‑298 (MQ=18)                                                                                                                                                                                                                                                                                     cACGGGGATCGGGCAGGGCTTGGGCCCCGCAGCCGCGATATCCTGAAATCCCGCCAGGTTGGCGCGACGTTTTGTTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAATTGTCCTGGGCAATAACGGCGCAAAACTGATTTTTCTCGGCACCAACTCCAACACCGCGCAGAGCCATAACGGCGAcc          <  2:917824/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                       cTGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGCTATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGCACTGACCGGTGACCCAATTGTCCTGGGCAATAACGGCGCAAAACTGATTTTTCTCGGCACCAACTCCAACACCGCGCAGCGCCATAACGGCGACCTg        >  1:451060/1‑298 (MQ=16)                                                                                                                                                                                                                                                                                        tGGTATCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAATTGTCCTGGGCAATAACGGCGCAAAACTGATTTTTCTCGGCACCAACTCCAACACCGCGCAGAGCCATAACGGCGACCTGt       <  1:1017430/298‑1 (MQ=18)                                                                                                                                                                                                                                                                                             tCGCGCAGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCCATTGTCCTGGGCAATACCGGCGCAAAACCGATTTTTCTCGGCACCAACCACAAAACCGCGCAGAGCCATCACGGCGACCCGTaatcc  >  2:417982/1‑294 (MQ=11)                                                                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                                                                     TCGAAATGAGTCTGGAAGCGCGGCTGACCCAGCTCATCATCAAACCGCAGAAAACCGGCGGTGACTTCAAGGAAATTGACCTGCTCGGAC‑GCCAGATTGAACGACTGGCACGGGTCAACCGTTACAGTCAGACCGGCAACGAGGCAGACCTTAATCCGAACGTCGCTAACCGCAACAAAGGCGGGCGTCGCAAACCGAAAAAGAATTTTTTCAGTGACGAGGCCATCGAAAAGCTGGAGCAGATTTTCTTTGAGCAGTCTTTCGAATATCAGTTGCACTGGTATCGCGCCGGGCTTGAGCACCGCATCCGCGATATCCTGAAATCCCGCCAGATTGGCGCGACGTTTTATTTTTCCCGCGAGGCGCTGCTGCGCGCCCTGAAAACCGGTCATAACCAGATTTTTCTGTCGGCCAGTAAAACGCAGGCGTATGTGTTCCGCGAATACATCATCGCCTTTGCCCGG‑CTGGTTGACGTTGACCTGACCGGTGACCCAATTGTCCTGGGCAATAACGGCGCAAAACTGATTTTTCTCGGCACCAACTCCAACACCGCGCAGAGCCATAACGGCGACCTGTACGTC  >  CP006584/2265596‑2266176

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑