Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	793,877	T→C	C358R (TGT→CGT)	LY180_03720 →	rhsA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	793,877	0	T	C	100.0%	66.8 / NA	29	C358R (TGT→CGT)	LY180_03720	rhsA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/15);  total (14/15)

TATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAAAAAGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAGTACAGCCCGGATGTGGTGACGGGCCTCATCA  >  CP006584/793781‑794165                                                                                                 |                                                                                                                                                                                                                                                                                                  tgaggccGGAGCAATCCGCGGGTGCGCCGCGTAACGTGCGAGGAGAAATGACGGGGCGGGAGGGTGGCGCCCGGTCACACGGGCCGACCGGAAGTCCGTTACCGTGACGACGGCGACGGGCGGGT‑GACAGAACAGCTAACCCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGt                                                                                         <  2:241660/293‑1 (MQ=11)   tGACCGCAGCAATACAAAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGttt                                                                                       <  1:571642/298‑1 (MQ=9)     aCCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGa                                                                                     >  1:483152/1‑298 (MQ=14)        gcgggaaTAAACCGGGGGGCAGGCTTACTTACGAGGATAAGTACCGGGGGGGGAGGGGGGGGCCCGGGCAAACGGGCGGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GAGAGAACAGCTAAACCCGGCAGGCTTAAGCTAGACGTATCAGGATGAGAAAGACCGCATCACCATCGCCGACAGCCTGGACCGCCGTGAAGGGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGc                                                                                  <  2:834798/293‑1 (MQ=11)          agcaATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGGGAAGTGCTGCACACGCAGGGCCAAGCCGGGCTGAAGCGGGTGGGGAAAAAGGAACACGCGGAAGGCAGCGTCACAAAGAGGCAGTTTGAAGCCg                                                                                >  1:1015325/1‑298 (MQ=11)               tACACAGGTGCGCGGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGc                                                                           >  1:626208/1‑298 (MQ=12)                  acaGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAgg                                                                        <  1:696033/298‑1 (MQ=17)                      gTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCa                                                                    <  1:945686/298‑1 (MQ=17)                       tGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGTACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCAGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCCCAg                                                                   >  1:1019943/1‑298 (MQ=12)                        gcgcAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAgg                                                                  <  1:831716/298‑1 (MQ=18)                          gcAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTTCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCTGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGc                                                                >  1:450705/1‑298 (MQ=255)                           cAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGca                                                               <  1:692420/298‑1 (MQ=21)                               tttACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGa                                                           >  1:35490/1‑298 (MQ=21)                                ttacttccGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACTACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGAc                                                          <  1:418544/298‑1 (MQ=255)                                    ttacGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGACGGAt                                                      <  1:546740/298‑1 (MQ=21)                                         atgatAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGGGGTGACAAAGGAACACGCGGACGCCAGCGGCACACAGCGCCCGTTTGACGCCGGGGGGAGGCCCAGGGACCAGGCGGagacccg                                                 >  2:811595/1‑292 (MQ=11)                                          tgatAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGACGGATGCCgca                                                >  1:878348/1‑298 (MQ=21)                                          ggagtaaggccGGGGCCGGGTGGGGGGGCACCGGCCGACGGGGCGGTGGGAAATCCGGTGCCGGTAGGAGGGCGGCGGGCGGGG‑GACAGGACAGCTAAACCCGGCAGGCGTAAGCGACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGACGGATGCCgca                                                <  2:705053/289‑1 (MQ=11)                                            atAAATACCGGGGCCGGGTGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGACGGATGCCgcagg                                              >  1:811211/1‑298 (MQ=19)                                             tAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGACGGATGCCgcaggc                                             <  1:570900/298‑1 (MQ=21)                                              aaaTACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCGACACGGATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCCGGACCGCCGTGAAGTGCTGCACACCCCGGGCGAAGCCGGGCGGAAGCGGGTGGTGAACAAGGAACCAGCGGACCGCGGCGTCACACAGAGGCAGCCTGACGCCGTGGGCAGGCACAGGGCACACGCGGaacgcgcacggc                                            >  2:362854/1‑287 (MQ=11)                                                        gCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTCAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGCAACCCTGGACCGCCGTGAAGTGCTTAACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGCAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGAGTGGCGGCTCACGGCACAGACGGCAGCCGCAGCCAGCacaacaaa                                  >  2:345179/1‑296 (MQ=11)                                                           ggATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGACTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAGTa                               <  1:359026/298‑1 (MQ=255)                                                             aTGGTGGCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAGTACa                             <  1:378703/298‑1 (MQ=255)                                                                gtggCGCACCGTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAGTACAGcc                          <  1:387969/298‑1 (MQ=255)                                                                     atctcGTCACACGGGCCGACCGGAAACCCCTTACCGTTACGGCAGCGAC‑GGCGGGTGGAAAGAACAGCTAACCCCTGCAGGCTTAAGCTACACGGATCAGTATGAGAAAGACCGCATCACCATCACCG‑‑AGCCTGGACCGCCGTGAAGTGCGGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAGTACAGCCCGGAtgt                   <  2:920306/294‑1 (MQ=11)                                                                          gTCACACGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCG‑‑AGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAGTACAGCCCGGAtgtgagat              >  1:920306/1‑294 (MQ=255)                                                                             acacGGGCCGACCGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGCCGGATGCCGCAGGCAGGACAACAGAGTACAGCCCGGATGTTGTGAc             >  1:738466/1‑298 (MQ=255)                                                                                        ccGGAAATCCGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGCTACACGTATCAGTATGAGAAAGACCGCATCACCATCACCGACAGCCTGGACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGACGGATGCCGCCGGCAGGCCAACAGAGAACAGCCCGGCAGTGGTGACGGGCCccacca  >  2:314309/1‑298 (MQ=11)                                                                                                 |                                                                                                                                                                                                                                                                                                  TATGACCGCAGCAATACACAGGTGCGCAGCTTTACTTACGATGATAAATACCGGGGCCGGATGGTGGCGCACCGTCACACGGGCCGACCGGAAATCTGTTACCGTTACGACAGCGACGGGCGGGT‑GACAGAACAGCTAAACCCGGCAGGCTTAAGTTACACGTATCAGTATGAAAAAGACCGCATCACCATCACCGACAGCCTGAACCGCCGTGAAGTGCTGCACACGCAGGGCGAAGCCGGGCTGAAGCGGGTGGTGAAAAAGGAACACGCGGACGGCAGCGTCACACAGAGTCAGTTTGACGCCGTGGGCAGGCTCAGGGCACAGACGGATGCCGCAGGCAGGACAACAGAGTACAGCCCGGATGTGGTGACGGGCCTCATCA  >  CP006584/793781‑794165

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑