Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	3,028,828	G→A	A112T (GCC→ACC)	LY180_14390 →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	3,028,828	0	G	A	89.5%	93.9 / NA	38	A112T (GCC→ACC)	LY180_14390	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (16/18);  minor base C (4/0);  total (20/18)
Fisher's exact test for biased strand distribution p-value = 1.07e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACCGGGATGCACTGGATGTGGCGCGAACACAGGCAGCGCTGTGGCAGACATGGCTGCTGCCGGTGACGGCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTGCTGGCCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGTGGCTGGCAGGGGAAAAGATGCTCGACAGCCTTGCCCGCTACCCGGAAGTGGCAAAAGAGGATTTTGC  >  CP006584/3028537‑3028967                                                                                                                                                                                                                                                                                                    |                                                                                                                                            gACCGGGATGCACTGGATGTGGCGCGAACACAGGCAGCGCTGTGGCAGACATGGCTGCTGCCGGTGACGGCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGAc                                                                                                                                       <  1:95006/298‑1 (MQ=255) gACCGGGAGGCAGGGGAGGTGGCGAGACCAGGGGTGGGGGTGTGGCTGACAGGGGTGCTGCGGGCGCAGGCAAAGACGCGGGTCGGGGAAGACCCCGGCTACGACGGCGATTTGCTGCGGATCCGCGACGAGATGAACAAGCTTACCGGCGCGGGTACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGGCGCAGGCAAAAGACGTGCGCGTCGCCACTGACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGAc                                                                                                                                       <  2:272462/298‑1 (MQ=17)  aCCGGGATGCACTGGATGTGGCGCGAACACAGGCAGCGCTGTGGCAGACATGGCTGCTGCCGGTGACGGCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACt                                                                                                                                      >  1:249935/1‑298 (MQ=255)         tggtctgggggtgGGGGGAACACAGGCCGCCGTGTGGCGGCGAGGGCTGCTGCCGGGGACGGCCGATACGCCGGTCGGGGAAGCCCCCGGATACCACGTCTATTTCCTGCGTATCCGCGACGAGATGAACAAACGGTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGaa                                                                                                                               <  2:885997/288‑1 (MQ=17)         tGCACTGGATGTGGCGCGAACACAGGCAGCGCTGTGGCAGACATGGCTGCTGCCGGTGACGGCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGaa                                                                                                                               >  1:176363/1‑298 (MQ=255)            aCTGGATGTGGCGCGAACACAGGCAGCGCTGTGGCAGACATGGCTGCTGCCGGTGACGGCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACGACCTCTGGGCGCGACTGCACCGCGACGGTGAGCGCTGACTGGACGAGGGGCTGGCGCTACTGCCCGGACTTGTGGGACGc                                                                                                                            >  1:640896/1‑298 (MQ=255)              ggggtgtgGCGCGAAAACAGGCAGCGCGGTGGCAGACATGGCTGCGGCCGGGGACGGCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCtt                                                                                                                          <  2:312142/294‑1 (MQ=255)                gATGTGGCGCGAACACAGGCAGCGCTGTGGCAGACATGGCTGCTGCCGGTGACGGCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCg                                                                                                                        <  1:266211/298‑1 (MQ=255)                            aaccaGGCACCGCTGTGGCAGACATGGCTGCTGCCGGTGACGGCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAActgc                                                                                                            <  1:416093/295‑1 (MQ=255)                             cacaGGCAGCGCTGTGGCAGACATGGCTGCTGCCGGTGACGGCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGCTACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCCGCTGACGCAGGCACACGACCTGCGCATCGCCCCCTACTCCCTCCGGGCGCCCCTGCACCCACACGGTCGCCCCCGACCGCCCGACGGGCCGGCGCTCCTGCCCGGCTCGGGGTGACGCTTCCGTCCGCacatccc                                                                                                           >  2:390929/1‑292 (MQ=21)                              acaGGCAGCGCTGTGGCAGACATGGCTGCTGCCGGTGACGGCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAActgctg                                                                                                          <  1:358099/298‑1 (MQ=255)                                 ggCCGCGCTGTGGCAGACATGGCTGCTGCCGGTGACGGCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACCAACTTTCCGGCGCGGATACGGACCTCATCTGCCCGCCTGCCGAACCACTCCTGCCGACGCAGGCAAAAGACGTGCCCATCGCCACTTCACACATCTGGGCGCGACTGCACCGCGACGCTGACCCCGGACCTGCGGACGGGCTGGAGCCACTGACCGGCCTGGGCGCACGCCTCGTTACGGCCCTGCTcccc                                                                                                       >  2:886194/1‑298 (MQ=255)                                                          ctgcCGGTGACGGCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTCCACCTGGGCGCGACTGCACCCCCACGGTGGGCGCGGCCTGGCTGAGGGGCTGGCGCTACTGCCCGGACTGGTTGAACCCTCCGGTACGCAACTGCCGCCCCCGCCCCCGGccccgcggcaaacgg                                                                              >  2:254873/1‑285 (MQ=18)                                                                gTGACGGCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTgg                                                                        >  1:976516/1‑298 (MQ=255)                                                                gTGACGGCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTACCCGCGACGAGATGCACACACTTTCCGGCGCGCATACGGACCTCATCTGCCAGCTAGCCCAATACCTGCTGCTGACGCAGCCAAAAGACGTGCGCATCACCACTCACTACACCCACGCGCGACGCCACCGCGCCGGTGCGCGCGGACTGGCCGGGGGGCTTGACACACTGCACGGCCGGGGGGACCGCTTCGGGCCGCAATCACCACCCTCACCACCAGCCAGCCGaaacagagctccgg                                                                        >  2:1023037/1‑287 (MQ=255)                                                                  gACGGCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAACGCGGCCTGGCTGCGGGGGCGGCGCCCCTGACCGGCCCGGTGGAACGCTTCGGTACGCAACCTCTGCCCTCCCCGCCGGCCAGCCCGCAAACGGCGCCGGAg                                                                      >  2:69246/1‑298 (MQ=18)                                                                    cGGCAAATACGCCGGTCGGGGAAGACCCCGGTTACCACGACGATTTCCTGCGTATCCGCGACGGGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGtg                                                                    <  1:482754/298‑1 (MQ=255)                                                                    cGGCAAAGAGGCCGGTCGGGGAGAACCCCGGCGACCCCGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACGTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCGGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGtg                                                                    <  2:381928/298‑1 (MQ=17)                                                                     ggCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGAACGGGGGAACGCTTCGGTACGCACCTGCTGCCCCCGCGCCCGGCCCCCCGGGCACGGGCCCGGGAGtgg                                                                   >  2:625674/1‑298 (MQ=17)                                                                        aaaTACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGtggctg                                                                <  1:720151/298‑1 (MQ=255)                                                                         aaTACGTCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGgggctgg                                                               >  1:178421/1‑298 (MQ=255)                                                                                          aGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGTGGCTGGCAGGGGAAAAGATGCTc                                              <  1:801755/298‑1 (MQ=255)                                                                                              cccGGGTACCACCAGGATTGCGTGGGGGTCCACGAGGAGATGAACAAACTTTCCCGGGCGGATACGGACCTAAGCTGCCAGCTTGACGAATCCCTGCGGCTGACGCAGGCAAAAGACGTGCGGATCGCCACGTACTACATCTGGGGGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGATTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGTGGCTGGCAGGGGAAAAGATGCTCGACa                                          <  2:925762/298‑1 (MQ=11)                                                                                                 ggCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGTGGCTGGCAGGGGAAAAGATGCTCGACAGcc                                       <  1:583709/298‑1 (MQ=255)                                                                                                  gCTACCGCGACGATTGCCTGCGTACCCGCGAGGGAATGACACAACTTCCCGGGGCGGATAGGGACCTCATCTGCGAGCTTGGCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGTGGCTGGCAGGGGAAAAGATGCTCGACAGCCt                                      <  2:581017/298‑1 (MQ=11)                                                                                                   cTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGTGGCTGGCAGGGGAAAAGATGCTCGACAGCCtt                                     >  1:172601/1‑298 (MQ=255)                                                                                                     aCCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGTGGCTGGCAGGGGAAAAGATGCTCGACAGCCTTGc                                   <  1:620377/298‑1 (MQ=255)                                                                                                            cgaTTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGTGGCTGGCCGGGGAAAAGATGCTCGACAGCCTTGCCCGCTAc                            >  1:691519/1‑298 (MQ=255)                                                                                                                ttCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGACAATGGCGCTGGAGTGGCTGGCAGGGGAAAAGATGCTCGACAGCCTTGCCCGCTACCCgg                        >  1:211513/1‑298 (MQ=255)                                                                                                                    tGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGTGGCTGGCAGGGGAAAAGATGCTCGACAGCCTTGCCCGCTACCCGGAAGt                    <  1:254873/298‑1 (MQ=38)                                                                                                                      cGTACCCGCGACGAGACGAACAAACTTTCCGGCGCGGATACGGACCTAATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTCACTACATCCGGGCGCGACCGCACCGCGACGGTGCCGCCAGACTGGCTGCGGGGCGGGCCATACTGACCGGACTGGGGGAACGCTGCGGTACGAAACTGCTGCCCTCGCGACCGGCCAGCCGGAACACGGCGACCGAGTTCCGCGCCGGGGCCAACATGCTCGCACCCCTCGCGCGCTAGCCGGaacggc                  >  2:314124/1‑294 (MQ=2)                                                                                                                       gTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGTGGCTGGCAGGGGAAAAGATGCTCGACAGCCTTGCCCGCTACCCGGAAGTGGc                 >  1:456726/1‑298 (MQ=37)                                                                                                                             gcaacgaGATGAACAAACTTTCCGGCGCGTATCCGGACCGCATCTGACAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGAGCTGGAGTGGCTGGCAGGGGAAAAGATGCTCGACAGCCTTGCCCGCTACCCGGAAGTGGCAAAaga           <  1:747978/295‑1 (MQ=255)                                                                                                                                   agaTGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGTGGCTGGCAGGGGAAAAGATGCTCGACAGCCTTGCCCGCTACCCGGAAGTGGCAAAAGAGGAttt     <  1:486833/298‑1 (MQ=17)                                                                                                                                   agaTGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGTGGCTGGCAGGGGAAAAGATGCTCGACAGCCTTGCCCGCTACCCGGAAGGGGCAAAAGAGGAttt     >  1:322817/1‑298 (MQ=17)                                                                                                                                     aTGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGTGGCTGGCAGGGGAAAAGATGCTCGACAGCCTTGCCCGCTACCCGGAAGGGGCAAAGGAGGATTTTg   >  1:16139/1‑298 (MQ=14)                                                                                                                                      tGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGTGGCTGGCAGGGGAAAAGATGCTCGACAGCCTTGCCCGCTACCCGGAAGTGGCAAAAGAGGATTTTGc  >  1:176329/1‑298 (MQ=14)                                                                                                                                      tGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCCACTGACCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCCCGCGACCGGCCAGCCGGACAATGGCGCCGGAGTGGCTGGCAGGGGAAAAGATGCCCGACAGCCTTGCCCGCAACCCCGACGCGGCAAAaaagcgatgtgc  >  2:414686/1‑290 (MQ=11)                                                                                                                                                                                                                                                                                                    |                                                                                                                                            GACCGGGATGCACTGGATGTGGCGCGAACACAGGCAGCGCTGTGGCAGACATGGCTGCTGCCGGTGACGGCAAATACGCCGGTCGGGGAAGACCCCGGCTACCACGACGATTTCCTGCGTATCCGCGACGAGATGAACAAACTTTCCGGCGCGGATACGGACCTCATCTGCCAGCTTGCCGAATCCCTGCTGCTGACGCAGGCAAAAGACGTGCGCATCGCCACTTACTACATCTGGGCGCGACTGCACCGCGACGGTGAGCGCGGACTGGCTGAGGGGCTGGCGCTGCTGGCCGGACTGGTGGAACGCTTCGGTACGCAACTGCTGCCCTCGCGACCGGCCAGCCGGAAAATGGCGCTGGAGTGGCTGGCAGGGGAAAAGATGCTCGACAGCCTTGCCCGCTACCCGGAAGTGGCAAAAGAGGATTTTGC  >  CP006584/3028537‑3028967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑