Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	3,772,505	A→G	F313F (TTT→TTC)	LY180_18030 ←	glutamate decarboxylase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	3,772,505	0	A	G	100.0%	24.1 / NA	17	F313F (TTT→TTC)	LY180_18030	glutamate decarboxylase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (10/7);  total (10/7)

AATTCATAGTACTGTGCAATTACCTGACCCGCCGGGCGGGAGAAGTTGATGGCAAAAGTACCAATTTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTC  >  CP006584/3772452‑3772792                                                      |                                                                                                                                                                                                                                                                                                 aaTTCATAGTACTGTGCAATTACCTGACCCGCCGGGCGGGAGAAGTTGATGGCGAAAGTACCAATTTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATCgg                                             <  1:915347/298‑1 (MQ=14)  caggctgggaCTGTGCAAGTACCTGTACCGCCGGGCGGGTGAAGTTGATGGCGAAAGGACCAACTTGACCACCCAGGTAGGCAACGGTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCCCTGCTCGATTTCACACGCGGCAGGCGGAAGCCCCAGACGAGATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATCGGc                                            <  2:36810/289‑1 (MQ=11)         gTACTGTGCAATTACCTGACCCGCCGGGCGGGAGAAGTTGATGGCGAAAGTACCAATTTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATCGGCCTGGAAt                                     >  1:991484/1‑298 (MQ=14)             tgtgCAATTACCTGACCCGCCGGGCGGGAGAAGTTGATGGCGAAAGTACCAATTTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTTGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATCGGCCTGGAATTTAt                                 >  1:264227/1‑298 (MQ=14)               tgCAATTACCTGACCCGCCGGGCGGGAGAAGTTGATGGCGAAAGTACCAATTTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACAGCTGGCAGCGTCGATGTGCATGTCGATGTG‑GGTACCGGTATCGGCCTGGAATTTATcc                               >  1:640185/1‑298 (MQ=14)               ggCAATTACTCGACGCGCGGGGCGGGAGAAGTGCGTGGCGAAAGTTCAAATTTGACCACCCGGGCAGTCAGTGGTGAACCCCTGGTCCTTGGGGGGCGATTGTGCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGACAGACCGAATTTATTGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATACGGGGCGACGAACGGGGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATCGGCCTGGAATTTATcc                               <  2:212027/297‑1 (MQ=11)                      aCCTGACCCGCCGGGGGGGAGAAGTTGATGGCGAAAGTACCCATGTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATCGGCCTGGAATTTATCCAGCGCAt                        <  2:384268/298‑1 (MQ=14)                       ccTGCCGCGCCGGGCGGGAGAAGTTGATGGCGAAAGTACCAATTTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATCGGCCTGGAATTTATCCAGCGCATc                       <  1:747938/298‑1 (MQ=14)                       ccTGACCCGCCGGGCGGGAGAAGTTGATGGCGAAAGTACCAATTTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATCGGCCTGGAATTTATCCAGCGCATc                       >  2:932460/1‑298 (MQ=14)                            cccGCCGGGCGGGAGAAGTTGATGGCGAAAGTACCAATTTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGGATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCCGGCGGAAGTCCCAGACGATATCCGGGGCCACGAACGGGTCCAGGCAGCCACCGCCGGCAGCGTCGGTGTGCATGCCCGTGGCGGTTCCCGTTACCGGCGTGGAATTTCCCCAgcgctacgtac                   >  2:272392/1‑291 (MQ=11)                               gccgGGCGGGAGAAGTTGATGGCGAAAGTACCAATTTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATCGGCCTGGAATTTCTCCAGCGCATCGTGCAGCg               >  1:252143/1‑298 (MQ=14)                                  gggcgggAGAAGTTGATGGCGAAAGTACCAATTTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGtt            <  1:765674/298‑1 (MQ=14)                                  gggcgggAGAAGTTGATGGCGAAAGTACCAATTTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGtt            <  1:561815/298‑1 (MQ=14)                                       ggAGAAGTTGATGGCGAAAGTACCAATTTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTggg       >  1:696285/1‑298 (MQ=14)                                        gagaAGTTGATGGCGAAAGTACCAATTTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATTGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGa      >  1:35598/1‑298 (MQ=14)                                         agaAGTTGATGGCGAAAGTACCAATTTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGaa     >  1:433519/1‑298 (MQ=14)                                            aGTTGATGGCGAAAGTACCAATTTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTc  >  1:167366/1‑298 (MQ=14)                                                      |                                                                                                                                                                                                                                                                                                 AATTCATAGTACTGTGCAATTACCTGACCCGCCGGGCGGGAGAAGTTGATGGCAAAAGTACCAATTTGACCACCCAGGTAGTCAACGTTGAACACCAGTTCCTGCGGCAGCGCTTCTTCGTCACGCCAGATAACCCAGCCGCAGCCCAGCGGAGCCAGACCGAATTTATGGCCTGAAGCACTGATCGATTTCACACGCGGCAGGCGGAAGTCCCAGACGATATCCGGGGCGACGAACGGTGCCAGGAAGCCACCGCTGGCAGCGTCGATGTGCATGTCGATGTC‑GATACCGGTATCGGCCTGGAATTTATCCAGCGCATCGTGCAGCGGTTGTGGGAACTC  >  CP006584/3772452‑3772792

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑