Predicted mutation
evidence seq id position mutation annotation gene description
RA CP006584 2,483,647 G→A P236S (CCG→TCG)  LY180_11800 ← NADH:ubiquinone oxidoreductase subunit M

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0065842,483,6470GA97.7% 142.9 / NA 44P236S (CCG→TCG) LY180_11800NADH:ubiquinone oxidoreductase subunit M
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (28/15);  minor base C (0/1);  total (28/16)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGGCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGA  >  CP006584/2483352‑2483934
                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                               
cTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACa                                                                                                                                                                                                                                                                                               <  1:372777/298‑1 (MQ=255)
  ggcGGGGTAGATAGCAAAAAGCAGGAAGCCCATGTGGGAAACCGAGGTGTAGGCGGTCAGACGTTTGGTATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAAACCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACAtt                                                                                                                                                                                                                                                                                             <  2:530219/296‑1 (MQ=255)
       tgtAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGAc                                                                                                                                                                                                                                                                                        >  1:72526/1‑298 (MQ=255)
                              cccAGGTGGGAAACCGGGGTGTAGGCGATCAGGAGTTTGAGAGCCGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCa                                                                                                                                                                                                                                                                 <  2:432874/298‑1 (MQ=255)
                                              aGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTc                                                                                                                                                                                                                                                 >  1:380651/1‑298 (MQ=255)
                                                            aGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCCTCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGCACGCGAAGGCGATGAAGAAGCCCACCATTAAAAAGTATTCACCACCACTGGACa                                                                                                                                                                                                                                   >  2:324632/1‑298 (MQ=255)
                                                                      tatCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGA‑AACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTc                                                                                                                                                                                                                        <  1:522339/298‑1 (MQ=255)
                                                                        tCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTCGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGACGCCCAGCACTAACAGGTATTCCACCCCACTGGCCATTGGCgctttcc                                                                                                                                                                                                                       >  2:513316/1‑292 (MQ=255)
                                                                        ccgggcagggCGCAACCCATCCAGGCACCGGAGAAGATGACGATAACCCCCGGCCACGAAGCGATTGGCGCAAACTCGGCCGATGCGGTCGGGAACAGCGGCAGGGCGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTca                                                                                                                                                                                                                       <  2:73739/291‑1 (MQ=255)
                                                                          ggTCGGGGCGAAAGCCAGCCACGACCCGTAGAAGAGGCCGATAACCCCCAGCCACATAGCGATTGGGGCAAACTCGGCCGAGGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTcagc                                                                                                                                                                                                                     <  2:326420/298‑1 (MQ=255)
                                                                            tCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTcagcag                                                                                                                                                                                                                   <  1:106838/298‑1 (MQ=255)
                                                                                    aaaGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGCTTAGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCAGCCGGTAGCCAGCAATGCAGCGGAACCACCGACATTTTGACTGCGAAGGAGATGAAGAAGCCCAAAACTAACAGGTATTCCACCCCACTGGACATTGGCGCATTAAGCAGCTCTTAAt                                                                                                                                                                                                           >  2:197146/1‑298 (MQ=255)
                                                                                          aTCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGa                                                                                                                                                                                                     >  1:203166/1‑298 (MQ=255)
                                                                                           tCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAAGCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCTTATTCAGCAGCCCTTCATTCTTGaa                                                                                                                                                                                                    >  2:418057/1‑298 (MQ=255)
                                                                                             cACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAgg                                                                                                                                                                                                  >  1:345699/1‑298 (MQ=255)
                                                                                                   ccGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTCCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCCCTGGACATTGGCGTATTCAACAGCTCTTCATAGTTAAAGGTCCAGa                                                                                                                                                                                            >  2:156879/1‑298 (MQ=255)
                                                                                                        gaagaTGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGTGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACCTTGGCGTATTCAGCAGCTCTTCATGGTTGAAGGTCCGGACGCCg                                                                                                                                                                                       >  2:242862/1‑298 (MQ=255)
                                                                                                                cGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACA‑TTTGACTGC‑CAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTAATCAGCAGCCTTTCATAGTTGAAGGTCCATACGCCGGCCGCATTGt                                                                                                                                                                             >  2:266433/1‑298 (MQ=255)
                                                                                                                   tAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTCGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGAAGCCGGTCGCATTGTa                                                                                                                                                                            >  1:353915/1‑298 (MQ=255)
                                                                                                                          cAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACa                                                                                                                                                                     <  1:266499/298‑1 (MQ=255)
                                                                                                                                        ttGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAg                                                                                                                                                       <  1:43197/298‑1 (MQ=255)
                                                                                                                                                                  gggAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGCCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACCCCGGCCGCATTGTAGTTAACAAAAACAAGCGCCAGGACGGCAATCAACATCACCCGGCCAc                                                                                                                             >  2:87759/1‑298 (MQ=255)
                                                                                                                                                                     aaCAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCg                                                                                                                          >  1:424742/1‑298 (MQ=255)
                                                                                                                                                                            gCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGAAATCAACATCACCAGGGCCCTCGCCTGGgt                                                                                                                   >  2:287682/1‑298 (MQ=255)
                                                                                                                                                                                  agaAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCACGCAACTCGCCTGGGTGTaact                                                                                                             >  2:151435/1‑296 (MQ=255)
                                                                                                                                                                                          agcaAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACt                                                                                                     >  1:291015/1‑298 (MQ=255)
                                                                                                                                                                                              aaCCGCAAGGGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGACCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGt                                                                                                 <  2:499361/298‑1 (MQ=255)
                                                                                                                                                                                               aCCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGgtg                                                                                                                  <  1:249231/280‑1 (MQ=255)
                                                                                                                                                                                               aCCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGgtg                                                                                                                  >  2:249231/1‑280 (MQ=255)
                                                                                                                                                                                                                  aGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGttt                                                                             >  1:13949/1‑298 (MQ=255)
                                                                                                                                                                                                                         tCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTc                                                                      >  1:528772/1‑298 (MQ=255)
                                                                                                                                                                                                                              gcgAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGCTGGCAATCAACATCACCAGGCCACCCGCCTGGGTGTAAATGAAGAACTAGGTCGCCGCCGGTATACGCGTTTAACCCTCAAAgg                                                                 >  2:463708/1‑298 (MQ=255)
                                                                                                                                                                                                                                gAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCt                                                               >  1:361742/1‑298 (MQ=255)
                                                                                                                                                                                                                                     cAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGCGAGAGGGTTGATg                                                          >  2:273018/1‑298 (MQ=255)
                                                                                                                                                                                                                                          gAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACAGTTTGACTGCGACGGCGATGAAGCAGCCCAGCATTAACCGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCGGGCCACTCGCCTGGGTGCAACTGACCAACTTGGCTCCCGCCGACAACACCGTTTCCCCGCAAGAGGCCTTATGccccc                                                     >  2:317441/1‑298 (MQ=255)
                                                                                                                                                                                                                                             ccGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCcaca                                                  <  1:334202/298‑1 (MQ=255)
                                                                                                                                                                                                                                                           cggagggggcgcACCGGGAACCCAGGCATGCAGGGGAACCCCCGACATTTTGAATGCGAAGGCGATGAAGAAGCCCAGCAGTAACAGGTATGCCACACCACTGGACATTGGCGTATGCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAg                                    <  2:168309/290‑1 (MQ=255)
                                                                                                                                                                                                                                                                        tCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGcc                                                                                                                               <  1:200852/194‑1 (MQ=255)
                                                                                                                                                                                                                                                                        tCCGGTAGCCAGCCATGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGcc                                                                                                                               >  2:200852/1‑194 (MQ=255)
                                                                                                                                                                                                                                                                                ccagccaTGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCAtt               <  1:287682/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                                  agccaTGCAGCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTc             >  1:254505/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                                    ccaGGCAGCGGAACCACCGCAATTTGTACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTccc           <  2:13949/298‑1 (MQ=255)
                                                                                                                                                                                                                                                                                           gCGGAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCagaagaa    >  1:563898/1‑298 (MQ=255)
                                                                                                                                                                                                                                                                                             ggAACCACCGACATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCagaagaaga  >  2:482840/1‑298 (MQ=255)
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CTGCCGGTGTAGATAGCAATCAGCACGAAGCCCATGTGGGAAACCGAGGTGTAGGCGATCAGACGTTTGATATCGGTCTGGGCGAAAGCCATCCACGCACCGTAGAAGATGCCGATAACCCCCAGCCACATAGCGATTGGCGCAAACTCTGCCGATGCGTTCGGGAACAGCGGCAGGGAGAAACGCAGCAAACCGTAAGCGGCAGTTTTCAGCAAGATCCCCGCGAGGTCAACGGAACCGGCGGTCGGAGCCTGAGAGTGCGCATCCGGTAGCCAGCCATGCAGCGGAACCACCGGCATTTTGACTGCGAAGGCGATGAAGAAGCCCAGCATTAACAGGTATTCCACACCACTGGACATTGGCGTATTCAGCAGCTCTTCATAGTTGAAGGTCCAGACGCCGGTCGCATTGTAGTGAACAAAAACCAGCGCCAGGATGGCAATCAACATCACCAGGCCACTCGCCTGGGTGTAAATGAAGAACTTGGTTGCCGCCGTGATACGCGTTTTACCGTCAGAGGCTTTATGCCCCCACAGTGCGATCAGGAAGTACATCGGCACCAGCATCATTTCCCAGAAGAAGA  >  CP006584/2483352‑2483934

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑