Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	CP006584	814,037	G→C	T139S (ACC→AGC)	gltA ←	type II citrate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP006584	814,037	0	G	C	100.0%	203.8 / NA	59	T139S (ACC→AGC)	gltA	type II citrate synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (31/28);  total (31/28)

GAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAA  >  CP006584/813743‑814325                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                  gAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTaa                                                                                                                                                                                                                                                                                                <  1:242390/298‑1 (MQ=255)                    cATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCAt                                                                                                                                                                                                                                                                             >  2:533800/1‑298 (MQ=255)                                               ggTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGCTAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTg                                                                                                                                                                                                                                                  >  2:567077/1‑298 (MQ=255)                                               ggTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTg                                                                                                                                                                                                                                                  >  1:413091/1‑298 (MQ=255)                                                   cATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGAACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAAc                                                                                                                                                                                                                                              <  1:446481/298‑1 (MQ=255)                                                         actttccgaaaatggATTAAGTCCTTACGGTTCGCACGGCGGGGAGAACATGCTGTTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACCAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCAGGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACgg                                                                                                                                                                                                                                        <  2:21955/285‑1 (MQ=255)                                                         aCGTTCCAGAATCGGATTAACTTCATACGGTTCGAACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATTGCATACTTGTCACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATCTCACGATGACGAGGAATGTTAACATCCAGCGAGTCGTGCTAAAACGCCACCAGCGAGCCACTAATCCCACCCCTGACTGCCATTGGATGTTAGTCGCTGCGGAAACCGGGGAAAAGACgg                                                                                                                                                                                                                                        >  2:227752/1‑298 (MQ=255)                                                               cggAATCGGGTTAAACTCATTCGGTAGGCGCGGCGGGGAGAAGAGCAGTTTCAGGAAGTTACCGGCATAGGGGAGGTCGTGGCGTGGGTAAACAAATGGCTGACCAATGGAATACTTGTAAAAAAGCGCGGCCGTGGTCGGCAGTTTCGACAGCAGGCGGATGGCGGAAATTTCACGATGACGAGGGTTGTTAAAATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATc                                                                                                                                                                                                                                  <  2:209520/296‑1 (MQ=255)                                                                 gAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTg                                                                                                                                                                                                                                >  1:97137/1‑298 (MQ=255)                                                                  aaTCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGc                                                                                                                                                                                                                               >  1:223869/1‑298 (MQ=255)                                                                      ggATTAACTTCATACGGTTCGCACGGCGTGGAGCACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATCCCACAAATGACTGCCATTGGATGGAAGTCGAGACGGAAAGAGGGTAACAGAAGGGTAATCTGccagt                                                                                                                                                                                                                           >  2:577049/1‑294 (MQ=255)                                                                          tAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGAt                                                                                                                                                                                                                       <  2:223869/298‑1 (MQ=255)                                                                                 aTACGGGTCGCACGGGGTGGAGAACATCATATTCAGGAAGTTACAGGCATAGGATAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTa                                                                                                                                                                                                                <  2:340334/298‑1 (MQ=255)                                                                                          gcgcGGGGTGGAGAACATCATATTCAGGGAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTc                                                                                                                                                                                                      <  2:377138/295‑1 (MQ=255)                                                                                          gCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTc                                                                                                                                                                                                      <  1:411551/298‑1 (MQ=255)                                                                                            aCGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATTGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCCACGCTAATACCACACATGACAGCCATTGGAGGGGAGTCGCGACGGAACGCGTGGAACCGGCGGGTAACCAGGCCGTGGGACATGGTTTGCACGGGTCa                                                                                                                                                                                                     >  2:55077/1‑298 (MQ=255)                                                                                            aCGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTCCACGATGACGAGGATTGTTAACATCCAGCGAGCCGTCATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGCGTGGCGACGGAAAGCGTGGAACAGACGGGTAATCTGATCGTGGATAAAGTGATG‑CCGGGTCAc                                                                                                                                                                                                    >  2:405798/1‑298 (MQ=255)                                                                                               gCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGt                                                                                                                                                                                                 >  1:328696/1‑298 (MQ=255)                                                                                                  tGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGCTTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCCCACATGACTGCCATTGGATGTGAGTCCCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGCTCGAGGTTGT‑ACGGGTCACCGAAGt                                                                                                                                                                                              >  2:345672/1‑298 (MQ=255)                                                                                                    gagaACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCCTTGGATGTGAGTCGCGACGGAAAGCGGGGAACCGACGGGTAATCTGCTCGTGGAACCTGGGATG‑ACGGGTCCCCGAAGgtt                                                                                                                                                                                            >  2:293208/1‑298 (MQ=255)                                                                                                            caaatTCAGGAAGTTACCGGCGTGAGAGAGATCGGTGCGGGGAGAAGCAAATGGCTGGACAATGGAAGACTTTGAACACATCGGGGCCATGGTCGGCATTTTCGGCAGCAGGCGGAAGGCGGCAATTTCACGATGGCGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCg                                                                                                                                                                                    <  2:396538/295‑1 (MQ=255)                                                                                                             atatTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGt                                                                                                                                                                                   <  1:162267/298‑1 (MQ=255)                                                                                                                ttCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCAt                                                                                                                                                                                <  1:267552/298‑1 (MQ=255)                                                                                                                    ggAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGCTTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATCCCACACATGACTGCCATTGGATGTGAGTCGCGCCGGAAAGCGGGGCACAGACGGGTAACCTTCTCGCGGTACATGGTGTG‑ACGGGCCACCCAGGGTTTAAATTTCTCAtcatg                                                                                                                                                                            >  2:511042/1‑294 (MQ=255)                                                                                                                     gacgTTACCGGCGTGGGGGAGGTCTTTGCGCGGCTAAACAAGTGGCTGCCCAATGGAATACTTGTAACGCATGGGGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGGGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGGGTCGTGAGAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCGTGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGt                                                                                                                                                                           <  2:310720/295‑1 (MQ=255)                                                                                                                      aaGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGtt                                                                                                                                                                          <  1:379918/298‑1 (MQ=255)                                                                                                                         ttACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCt                                                                                                                                                                       >  1:385957/1‑298 (MQ=255)                                                                                                                         ttACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAAAAGACGGGTAATCTGCTAGTTGATCATGGTATT‑GCGGGTCCCCGGAGTTTTAAATTCGGCATACTTTTCCt                                                                                                                                                                       >  2:202632/1‑298 (MQ=255)                                                                                                                                    ggAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCCATTTCACGATGACGAGGATTGTTCACATCCCGCGAGTCGTGATAGAACGCCTCCAGCGCGCCGCTACTACCACACCTGCCTGCCACTGGATGTGACTCGCGACGGAAAGAGTGGCACAGAAGGATAGTCTTCCCGTGGTCCATGGTATT‑GCGGGTCACAGTAGGTTTAAATTCGTCATACTTTTCCTCCATCGGtttg                                                                                                                                                            >  2:30907/1‑297 (MQ=255)                                                                                                                                    ggAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGCCGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGACCATGGTATG‑ACGGGTCCCCGGAGTTTTAAATTCGACATACTGTTCCTGAGTCCGtttt                                                                                                                                                            >  2:409512/1‑298 (MQ=255)                                                                                                                                     gagagaACGTGGCGCGGATAAAAAAATGGGTGAGAAATGGAATACTTGGAACAAATCGCGGCCATGGTCGGCATTTTCGACAGCCGGCGGCACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGttttt                                                                                                                                                           <  2:217319/298‑1 (MQ=255)                                                                                                                                       gagaTCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGACATACTGTTCCTGAGTCGGTTTTTCa                                                                                                                                                         >  1:158474/1‑298 (MQ=255)                                                                                                                                         gaTCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCAcc                                                                                                                                                       >  1:390046/1‑298 (MQ=255)                                                                                                                                             gTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCACGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTc                                                                                                                                                   >  2:147000/1‑298 (MQ=255)                                                                                                                                                     gATAAAAAAATGGCGGACCAATGGAATACGTGTAACCCATCGCGGCCATGGTCGGCATTTTCGACATCAGGCGGAACGCGGCAAGTTGACGATGACGAGGATTGTGAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGAt                                                                                                                                           <  2:464988/298‑1 (MQ=255)                                                                                                                                                         aaCAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTaa                                                                                                                                       >  2:121115/1‑298 (MQ=255)                                                                                                                                                          aCAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAAc                                                                                                                                      <  1:526121/298‑1 (MQ=255)                                                                                                                                                          aCAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAAc                                                                                                                                      >  1:518165/1‑298 (MQ=255)                                                                                                                                                                   tGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCa                                                                                                                             <  2:378179/298‑1 (MQ=255)                                                                                                                                                                         aTGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGt                                                                                                                       <  1:101836/298‑1 (MQ=255)                                                                                                                                                                         aTGGAATACTTGCAACCAAACGGGGGCATGGTGGGCAGTTTGGACGGCGTGCGGAACGCGGGAATTTCACGATGACGAGGATTGTTAACAGCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACGCATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGt                                                                                                                       <  2:510857/298‑1 (MQ=255)                                                                                                                                                                                      aaCACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGACGGCTGTAACAAACTTCCAGGTAGTTAGACTCGGTccc                                                                                                          >  2:89927/1‑296 (MQ=255)                                                                                                                                                                                        cacaTCGCGGCCATGGTCGGCATTTTCGAAAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGAGAGAACGCCGCCAGCGCGCCGCTAATACCACACATGGCTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCa                                                                                                        <  2:96536/298‑1 (MQ=255)                                                                                                                                                                                           aTCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCt                                                                                                     <  1:556897/298‑1 (MQ=255)                                                                                                                                                                                                ggCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcg                                                                                                >  1:428904/1‑298 (MQ=255)                                                                                                                                                                                                   cATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTgatcgatc                                                                                             >  2:545602/1‑298 (MQ=255)                                                                                                                                                                                                    aTGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGatcgatcg                                                                                            >  1:450067/1‑298 (MQ=255)                                                                                                                                                                                                          ggCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAAc                                                                                      >  2:497097/1‑298 (MQ=255)                                                                                                                                                                                                                 tCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCCCCAGCGCGCCGCTAAGACCCCACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCTTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTg                                                                               <  1:30907/298‑1 (MQ=255)                                                                                                                                                                                                                   acaggcagGCGGAACGCGGCAATTGCCCGATGCCGAGGATTTTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGAGCGCCGCTAATACCACACATGACTGCCCTTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTgca                                                                             <  2:385957/294‑1 (MQ=255)                                                                                                                                                                                                                     cagcagGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTgcagc                                                                           >  1:7317/1‑298 (MQ=255)                                                                                                                                                                                                                            cGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATAc                                                                    <  1:533800/298‑1 (MQ=255)                                                                                                                                                                                                                                                        gATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAAtttt                                        >  1:253953/1‑298 (MQ=255)                                                                                                                                                                                                                                                                    ccAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGa                            <  1:409512/298‑1 (MQ=255)                                                                                                                                                                                                                                                                          aGTCGTGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGt                      <  2:97137/298‑1 (MQ=255)                                                                                                                                                                                                                                                                               tGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTtgaag                 <  1:147000/298‑1 (MQ=255)                                                                                                                                                                                                                                                                               tGATAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTtgaag                 <  1:121115/298‑1 (MQ=255)                                                                                                                                                                                                                                                                                  tAGAACGCCGCCAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTtgaagtga              >  1:542410/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                             cAGCGCGCCGCTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCA‑CATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCCCCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCaa  >  2:135299/1‑298 (MQ=255)                                                                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                                                                  GAGGTAGAGGCGTTCTGTTCATGGTCAGCGTGCAGGATCAGAATACGGTCCATAGCACGTTCCAGAATCGGATTAACTTCATACGGTTCGCACGGCGTGGAGAACATCATATTCAGGAAGTTACCGGCATAGGAGAGATCGTTGCGCGGATAAACAAATGGCTGACCAATGGAATACTTGTAACACATCGCGGCCATGGTCGGCATTTTCGACAGCAGGCGGAACGCGGCAATTTCACGATGACGAGGATTGTTAACATCCAGCGAGTCGTGATAGAACGCCGCCAGCGCGCCGGTAATACCACACATGACTGCCATTGGATGTGAGTCGCGACGGAAAGCGTGGAACAGACGGGTAATCTGCTCGTGGATCATGGTATG‑ACGGGTCACCGTAGTTTTAAATTCGTCATACTGTTCCTGAGTCGGTTTTTCACCATTCAGCAGGATGTAACAAACTTCCAGGTAGTTAGAATCGGTCGCCAGCTGATCGATCGGGAAACCGCGGTGCAGCAAAATACCTTCATCACCATCAATAAAAGTAATTTTAGATTCGCAGGATGCGGTTGAAGTGAAGCCTGGGTCAA  >  CP006584/813743‑814325

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑