New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2647275 =	65 (0.890)	6 (0.090)	6/278	6.4	8.9%	coding (51/2313 nt)	pbpC	penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
	?	NC_000913	2647305 =	62 (0.900)					coding (21/2313 nt)	pbpC	penicillin‑insensitive murein repair transglycosylase; inactive transpeptidase domain protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below cutoff threshold.

GACGCCGGGAACGTATCAGGTACCGCAACCGATGGTGGAATCAATGTATGTTCCCCAATGGCGGGCGACCGGCGCGGCTGAAGATCTGCTGATTGTCAGACCGTAAATGCCTCGCTTGTTAACCAAACGCGGCTGCTGGATAACGTTGGCAGCCGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2647431‑2647275 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcagccgcgTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCCGCGCCAGATACACC  >  NC_000913/2647305‑2647452                                                                                                                                                                                                                                                                                                                   GACGCCGGGAACGTATCAGGTACCGCAACCGATGGTGGAATCAATGTATGTTCCCCAATGGCGGGCGACCGGCGCGGCTGAAGATCTGCTGATTGTCAGACCGTAAATGCCTCGCTTGTTAACCAAACGCGGCTGCTGGATAACGTTGGCA                                                                                                                                                            >  1:314769/1‑151        GGAACGTATCAGGTACCGCAACCGATGGTGGAATCAATGTATGTTCCCCAATGGCGGGCGACCGGCGCGGCTGAAGATCTGCTGATTGTCAGACCGTAAATGCCTCGCTTGTTAACCAAACGCGGCTGCTGGATAACGTTGGCAGCCGCG                                                                                                                                                      >  1:483982/1‑150                      ACCGCAACCGATGGTGGAATCAATGTATGTTCCCCAATGGCGGGCGACCGGCGCGGCTGAAGATCTGCTGATTGGCAGGCCGGAAATGCCTCCCTTGTTAACCCAACGCCGCTGCTGGCTAACGTTGGCAGCCGCGTTTGGTTAACAAGCG                                                                                                                                       >  2:626517/1‑151                                ATGGTGGAATCAATGTATGTTCCCCAATGGCGGGCGACCGGCGCGGCTGAAGATCTGCTGATTGTCAGACCGTAAATGCCTCGCTTGTTAACCAAACGCGGCTGCTGGATAACGTTGGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTA                                                                                                                              <  1:988775/150‑1                                   GTGGAATCAATGTATGTTCCCCAATGGCGGGCGACCGGCGCGGCTGAAGATCTGCTGATTGTCAGACCGTAAATGCCTCGCTTGTTAACCAAACGCGGCTGCTGGATAACGTTGGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGG                                                                                                                           <  2:93943/150‑1                                               TATGTTCCCCAATGGCGGGCGACCGGCGCGGCTGAAGATCTGCTGATTGTCAGACCGTAAATGCCTCGCTTGTTAACCAAACGCGGCTGCTGGATAACGTTGGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGC                                                                                                              <  1:626828/151‑1                                                          ATGGCGGGCGACCGGCGCGGCTGAAGATCTGCTGATTGTCAGACCGTAAATGCCTCGCTTGTTAACCAAACGCGGCTGCTGGATAACGTTGGCAGCCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGC                                                                                                   <  1:229534/151‑1                                                                                                       GTAAATGCCTCGCTTGTTAACCAAACGCGGCTTCTGGAGAACGTTAGCAGTCGCGTTTGGTTAACAAGCGAGGCACTTCCGGTCTCACAATGAGCAGATCTTCAGCCGCCCCGGTCGCCCTCCATTTGGGTACATACCATGCTTCCATCAT                                                      >  2:128386/1‑151                                                                                                                                                         CCGCGTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCCGCGCCAGATACA    <  1:36331/151‑1                                                                                                                                                            CGTTTGGTTAACAAGCGAGGCATTGACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTTCCTGATACGTTCCCGGCGTCACCGCCCGCGCCAGATACACC  <  1:312548/150‑1                                                                                                                                                                                                                                                                                                                   GACGCCGGGAACGTATCAGGTACCGCAACCGATGGTGGAATCAATGTATGTTCCCCAATGGCGGGCGACCGGCGCGGCTGAAGATCTGCTGATTGTCAGACCGTAAATGCCTCGCTTGTTAACCAAACGCGGCTGCTGGATAACGTTGGCAGCCGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2647431‑2647275 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gcagccgcgTTTGGTTAACAAGCGAGGCATTTACGGTCTGACAATCAGCAGATCTTCAGCCGCGCCGGTCGCCCGCCATTGGGGAACATACATTGATTCCACCATCGGTTGCGGTACCTGATACGTTCCCGGCGTCACCGCCCGCGCCAGATACACC  >  NC_000913/2647305‑2647452

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.