Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4960TA45.3% 62.4 / 30.2 53intergenic (+301/‑155)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (25/4);  new base A (0/24);  total (25/28)
Fisher's exact test for biased strand distribution p-value = 3.71e-11
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAG  >  NC_000913/29346‑29644
                                                                                                                                                         |                                                                                                                                                    
tttAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAatat                                                                                                                                                      <  1:809250/151‑1 (MQ=255)
  tAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                                    <  2:854389/151‑1 (MQ=255)
         aaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATcc                                                                                                                                             <  2:700374/151‑1 (MQ=255)
                                         cGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                               >  1:804667/1‑149 (MQ=255)
                                         cGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                               >  1:350458/1‑149 (MQ=255)
                                           tttttATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAcctc                                                                                                            >  1:986086/1‑150 (MQ=255)
                                             ttttttTGATGCAATTTTTTTGCTGCGCAAGCTTTTCCAAAAAAAGTTAAGATGATTTTTTTTTCGCTTAAGCCTTGTAAAAAATGCATGGCCCACAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAg                                                                                                         <  2:777337/151‑1 (MQ=255)
                                             tttATTTCATCCAATTTTCTGGCTGCACAAGCTTTTCCAAGAACAGGTTAAATTATTTTTTTTTCGCTTAATGCTTAAAAACAATGCATGCCCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAg                                                                                                         <  2:1146448/151‑1 (MQ=255)
                                                aTTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAACCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAAt                                                                                                      >  1:968195/1‑151 (MQ=255)
                                                          aTCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                            >  1:447335/1‑151 (MQ=255)
                                                          aTCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                            >  1:497343/1‑151 (MQ=255)
                                                            cttcttGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTg                                                                                           >  1:1058142/1‑150 (MQ=255)
                                                                ttgccgccgaaggcttttCCAAGAACGGTATAGATATTTTTTTTTTCGTTAAAGGCTTGAAAAACATGAATGAACCAAAAAAAAAAAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAg                                                                                       <  2:838768/137‑1 (MQ=255)
                                                                   ctgcGCAAGCGTTTCCAAAACAAGTTTAAATGATTTTTTTGCCGATAAATCCTTAAAAAACAGGAATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaaa                                                                                    <  2:52967/150‑1 (MQ=255)
                                                                   ctgcACAAGCTTTTTCCAGAACAGGTTAGATGATTTTTTTGTCGCTTAATCCCTGAAAAACATGAAGGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAt                                                                                   <  1:1002637/151‑1 (MQ=255)
                                                                     gcgcAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAAtt                                                                                  >  1:459962/1‑150 (MQ=255)
                                                                          aGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCgg                                                                            >  1:970442/1‑151 (MQ=255)
                                                                              ttttcaaaGAACAGGTTAGATGTTTTTTTTGTCTCTTAATGCCTGTAAAACATGCATGACCCAAAAAATAAAAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTaa                                                                         <  2:829607/144‑1 (MQ=255)
                                                                                  tcaaaaaCAGTTTAGATGTTTTTTTTGCCGTTTAATGCCTAAAAAACATGCATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAg                                                                     <  1:166983/146‑1 (MQ=255)
                                                                                            ggTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAtt                                                            >  1:733061/1‑151 (MQ=255)
                                                                                                      atCTTTTTGTCGTTAAAGGCCTGTAAAACGTGCAGGTGAAACAAAATAATAAAAAAAAGCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                   <  2:51516/151‑1 (MQ=255)
                                                                                                      atCTTTTTGTCGCGAAATCCCTGAAAACCATACATGACCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                   <  2:1040018/151‑1 (MQ=255)
                                                                                                       tCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                   >  1:406441/1‑150 (MQ=255)
                                                                                                       tCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                   >  1:733914/1‑150 (MQ=255)
                                                                                                        cTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtca                                                 >  2:194764/1‑151 (MQ=255)
                                                                                                          ttttttCGCTTAATCCTTAAAAAACATGAAGGAGCCAAAAAAAAAAAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatc                                               <  1:872210/151‑1 (MQ=255)
                                                                                                           tttGTCCTTTATTCCCTTAAAAACATGCTGGACCCAAAAAATATTATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGtcatca                                              <  1:807387/151‑1 (MQ=255)
                                                                                                            ttGTCGTTTAATGCTTAAAAACCATGAAGGACCAAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                             <  1:89998/151‑1 (MQ=255)
                                                                                                            ttGTCGTTTAATCCTTAAAAAACTTGCAGGGCCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTcatcat                                             <  2:519643/151‑1 (MQ=255)
                                                                                                               tCGCTTAGTCCCTGAAAACAATGCATGAGCAAAAAAAAAAAAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtg                                           <  2:782232/150‑1 (MQ=255)
                                                                                                                cGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATtgtg                                         >  1:711832/1‑151 (MQ=255)
                                                                                                                 gattACTGCCTGTAAAACATGCATGAGCCACACAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCAAGAAATTCGTCGGTAATCAGATTTGCATTGATTTACGTCATCAGTGTGa                                        >  2:209044/3‑151 (MQ=255)
                                                                                                                 ctttattgtctaaaaaaCATGTAGGCACCAAAAAATAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGa                                        <  2:1138513/138‑1 (MQ=255)
                                                                                                                  cTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                                       >  2:882756/1‑151 (MQ=255)
                                                                                                                  cTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGaa                                       >  1:1064909/1‑151 (MQ=255)
                                                                                                                   taattccttatAAAACATGAGTGACCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAt                                      <  2:70727/141‑1 (MQ=255)
                                                                                                                    aaaggCCTGAAAAACTGGCAAGAGACACAAAATAAAAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTAAGTCATCATTGTTAAtt                                     <  2:270751/147‑1 (MQ=255)
                                                                                                                      ataccTGAAAAACATGTATGAGCCAAAAAATAATATAAAAAATCCCGCCATAAAGTTGACTTATAGGGGCCATATCTTCAGAATGCAGCCGTTTGCTAGAAATTCGTCTGTATGCAGATTTGCATTGATTTACGTCATCATTGTGCATTaa                                   >  2:706754/4‑151 (MQ=255)
                                                                                                                       ttcttaaaaacCATGCAGGAGCCAAAAAAAAATAAAAAAAAGACCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAat                                  <  1:1011606/144‑1 (MQ=255)
                                                                                                                        cccTGAAAAACATGAATGACCCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAata                                 <  1:633791/150‑1 (MQ=255)
                                                                                                                          cTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGcc                                                                                         >  1:34946/1‑93 (MQ=255)
                                                                                                                          cTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                               >  2:632872/1‑151 (MQ=255)
                                                                                                                          cTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATg                               >  2:1136072/1‑151 (MQ=255)
                                                                                                                                 aCATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaa                        >  1:596765/1‑151 (MQ=255)
                                                                                                                                   tttcgcaagtaacaAAATAATAAAAAAGCCCCCGCCATTAAGTTGACTTTTAACGCCCATATCTCCAGAATGCCGCTGTTTGCCAGACATTCGTCGGTAAGCAAATTTTCATTGATTTCCGTCATCATTGTGAATTAATATGCAAATAAag                      <  2:215384/140‑1 (MQ=255)
                                                                                                                                   atgcttgccccAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAag                      <  1:698450/142‑1 (MQ=255)
                                                                                                                                    tgagtgAGCAAGAAATAAAAATAAAAGAGCCCGCCATTAAGTTTACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCCTCATTGTGAATTAATATGCAAATAAag                      <  2:877510/146‑1 (MQ=255)
                                                                                                                                     gcatgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                    >  2:44338/1‑151 (MQ=255)
                                                                                                                                     gaaggaccaacaAAAAAATAAAAAAACTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAag                      <  2:732724/140‑1 (MQ=255)
                                                                                                                                            ccACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat             >  2:864399/1‑151 (MQ=255)
                                                                                                                                              aaaaaaaaATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAatat           <  1:1011027/149‑1 (MQ=255)
                                                                                                                                                 aaaTAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGTTTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGGGAATAtt          >  1:951164/1‑149 (MQ=255)
                                                                                                                                                  aataatAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtctc       <  1:369005/151‑1 (MQ=255)
                                                                                                                                                  aataaaAAAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtctc       <  2:1081694/151‑1 (MQ=255)
                                                                                                                                                      atatAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGa   >  1:812753/1‑151 (MQ=255)
                                                                                                                                                         taAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAg  >  1:1002427/1‑149 (MQ=255)
                                                                                                                                                         |                                                                                                                                                    
TTTAATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGAG  >  NC_000913/29346‑29644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: