Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,686,028	0	T	C	45.5%	62.8 / 16.6	55	E1049G (GAG→GGG)	bcsC	cellulose synthase subunit
Reads supporting (aligned to +/- strand):  ref base T (23/7);  new base C (1/24);  total (24/31)
Fisher's exact test for biased strand distribution p-value = 2.11e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGATCTTTGTCGTAATGCCAGATCATGTTGTTCAGGCCGATT  >  NC_000913/3685879‑3686174                                                                                                                                                        |                                                                                                                                                   gcgcCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCt                                                                                                                                                    >  2:372467/1‑150 (MQ=255)      gtgtAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACg                                                                                                                                              >  2:530405/1‑151 (MQ=255)                aGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGTCCACGAGCCAGACGCACCCAGCCCCCACGACCAGtttt                                                                                                                                     >  2:782599/1‑150 (MQ=255)                  cccTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTc                                                                                                                                    <  2:1089277/149‑1 (MQ=255)                    cTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTg                                                                                                                                <  1:368082/151‑1 (MQ=255)                             gccgccATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCg                                                                                                                        >  1:266483/1‑150 (MQ=255)                                 ccATCGTTGGTTTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCGGCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGCCCACGAGCCAGACGCCCCCAGCTCCCACGCCCAGTTTTCCGTGCGCTCCCGCcaca                                                                                                                   <  2:500570/151‑1 (MQ=255)                                    tCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCa                                                                                                                >  2:1139574/1‑151 (MQ=255)                                     ctttGGATTGCCGCGCCCCTTCTTCCGGCCAGTCGGTCGGGATCAGATTCACCGGCGGAAACCGCGGCATGTTTT‑T‑GGTGCGTGAATCCGCCCCCGAGCCAGACCCCCCCAGCCCCCCCGACCAGTTTCCCGTGCGCTCCCGCCACATCAc                                                                                                               <  2:302867/149‑1 (MQ=255)                                      ttggtatccgcgcgcgGCTTCTCCCGCCCGGGCGGCGGGAATCAGCTTCATCGGGGGAAACCGCGCCTTGGTTT‑T‑GGGGCGTAACTGCACCCCCGACCCACCCGCACCCACCTCCCCCGCCCATTTTCCGGTGCGCTCCCGCCACATCAc                                                                                                               <  2:862871/140‑1 (MQ=255)                                              gCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATgg                                                                                                       >  2:950795/1‑150 (MQ=255)                                                      gCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCACCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCCCCCGCCACATCACCGGTATGGCAACCGACa                                                                                              >  2:620903/1‑151 (MQ=255)                                                          cttcCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATTCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTa                                                                                          >  2:15515/1‑151 (MQ=255)                                                              cggCCGGTCGGTGGGGACCAGATTCACCAGCGGAAAACGCGGCATGGTTT‑G‑GGGGCGTAAATTCGACCACGAGCCAGACGCCCCCAGCCCCCACGACCGGTTTTCCGTGCGCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTcc                                                                                      <  1:15519/149‑1 (MQ=255)                                                              cTGCCAGTCGGTGGGGATCAGATTCATCGGCGGATACCGGGGCATGTTTT‑T‑GGTGCGTGAATGCGCCCCCGAGCCAGACGCCCCCAGCCCCCCCGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTcc                                                                                      <  1:8984/151‑1 (MQ=255)                                                                 ccAGTCGGTCGGGATCAGTTTCATCAGCGGATAACGCGGCATGTTTT‑T‑GGTGCGTGAATGCGCCCACGAGCCAGACGCCCCCAGCCCCCACGACCATTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTg                                                                                    <  1:1140103/150‑1 (MQ=255)                                                                     tcggtgggGATCAGATTCATCAGCGGATAACGCGGCATGGTTT‑T‑GGTGCGGGAATGCACCCACGAGCCAGACGCCCCCACCCCCCACGACCATTTTTCCGGGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGAc                                                                               <  1:1020775/151‑1 (MQ=255)                                                                         tcggGATCAGATTCATCGGGGGATAACGGGGCATGGTTT‑T‑GGTGCGTGAATGCGCCCCCGAGCCAGCCCCCCCCAGCCCCCCCGCCCAGTTTTCCGTGCGCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTgt                                                                            <  1:1050303/150‑1 (MQ=255)                                                                            ggctcagcctcatccCCGGATAACGCGGCATGGTTT‑T‑GGTGCGTCACTGCGACCACCAGCCAGACCCACCCCGCTCCCTCGACCAGTTTTCCGTGCGCTCCCGCCACCTCACCGGTACGGCACACCACAGGTATTCCTGCGGCCCgtagta                                                                        >  2:365851/10‑151 (MQ=255)                                                                            ggATCAGATTCATCAGCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAAc                                                                                                  >  1:48487/1‑125 (MQ=255)                                                                               tCAGTTTCATCAGCGGAAAACGCGGCATGTTTT‑T‑GGGGCGTGAAGGCGCCCACGAGCCAGACGCCCCCAGCCCCCCCGACCTTTTTTCCGTGCGCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAcc                                                                     <  1:934587/151‑1 (MQ=255)                                                                               tCAGATTCATCCGCGGAAAGCGCGGTTTGTTTT‑T‑GGGGGGTAAAGGCCCCCAGGCCCCAGGCGCCCCCCGCCCCCACCCCCTTTTTTCCGTGCCCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAcc                                                                     <  1:112835/151‑1 (MQ=255)                                                                               tCAGATTCACCGGGGGATAACGGGGCATGTTTT‑G‑GGGGCGTGAATGCGCCCACGGGCCAGACGCCCCCAGCCCCCACGACCGGTTTCCCGGGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAcc                                                                     <  1:399392/151‑1 (MQ=255)                                                                               tCAGATTCACCGGCGGATAGCGGGGCAGGGTTT‑G‑GGTGCGGGAAAGCCCCCCCGAGCCAGGCCCCCCCAGTTCCCCCGACCAGTTTTCCGTGCCCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAcc                                                                     <  1:1037398/151‑1 (MQ=255)                                                                               ccaaatccatcaGGGAAAACCGCGGCATGTTTT‑T‑GTTGCGTAAATGCGACCACGAGCCAGACGCCCCCAGCCCCCACGACCATTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAAcc                                                                     <  1:1156293/144‑1 (MQ=255)                                                                                  gATTCATCAGCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGc                                                                   >  2:507455/1‑150 (MQ=255)                                                                                   tttCACCAGGGGAAACCGCGGCTTGTTTT‑T‑GGTGGTTGAAGGCCCCCCCGAGCCAGACGCCCCCGCCCCCCCCCCCCAGTTTCCCGTGCCCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGccc                                                                 <  1:1010741/150‑1 (MQ=255)                                                                                       accaGGGGATAACGGGGCTTGTTTT‑G‑GGGGGGTAAATGCGCCCACGGGCCAGCCCCCCCCGGCCCCCACGCCCATTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGAc                                                             <  1:100750/149‑1 (MQ=255)                                                                                        tcaGCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGAc                                                             >  1:15300/1‑150 (MQ=255)                                                                                        tcaGCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGAc                                                             >  1:1138004/1‑150 (MQ=255)                                                                                        ccgggggATACCGGGGC‑TGGTTT‑TGGGTGCGTGAAGCCGACCCCGACCCAGGCGCACCCAGCTCCCCCGACCTGTTTCCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGAcc                                                            <  2:286766/146‑1 (MQ=255)                                                                                        ccaGCGAATACCGCGGCTTGTTTT‑T‑GTTGGGGAAATGCCCCCACGAGCCAAACCCCCCCAGCCCCCACGCCCTTTTTCCCGTGCGCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGAcc                                                            <  2:1059699/150‑1 (MQ=255)                                                                                         cgggggAAAACGCGGTGGGTTTT‑T‑GGGGCGTAAAGGCGCCCACGACCCAGCCGCCCCCAGCCCCCACGCCCAGTTTTCCGGGCGCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCg                                                           <  1:458371/147‑1 (MQ=255)                                                                                              gATAACGGGGCTTGTTTT‑T‑GGTGCGTGAATGCGCCCCCGAGCCAGACGCCCCCAGCCCCCACGACCAGTTTTCCGTGCGCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCgagtg                                                       <  1:178439/150‑1 (MQ=255)                                                                                              gATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGCATTCCTGCGGACTGTAGTAACCGCCCTGACCgagtga                                                      >  2:190468/1‑151 (MQ=255)                                                                                              aaaaaCGGGGCATTGTTT‑G‑GGTGCGAAAAGGCGCCCACGACCCACCCGCACCCGCCCCCCACGCCCATTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCgagtg                                                       <  1:803155/147‑1 (MQ=255)                                                                                                  ccgcgGAGTGTTTT‑T‑GGGGCGTAAATGCCCCCAGGGGCCAGACCCCCCCCGCCCCCACGCCCATTTTTCCGTGCCCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAg                                                  <  1:705443/150‑1 (MQ=255)                                                                                                  aCGCGGC‑TGGTTTGG‑GGTGGGTAAATGCGCCCCGGAGCCGGCCGCCCCCATCTCCCCCGCCCGGTTTTCCGGGCGCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAg                                                  <  1:967569/151‑1 (MQ=255)                                                                                                            tttt‑g‑GGTGCGGAAATGCGCCCCCGACCCAGCCCCCCCCCGCCCCCCCGCCCAGTTTTCCGTGCCCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGa                                         <  1:443941/149‑1 (MQ=255)                                                                                                            gTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCCCCGGGATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAg                                          >  2:668995/1‑149 (MQ=255)                                                                                                            gTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGAt                                        >  1:510973/1‑151 (MQ=255)                                                                                                            gTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGAt                                        >  1:1152155/1‑151 (MQ=255)                                                                                                             ttt‑t‑GGTGCGTGAGTCGGCCCGCGACCCAGCCGCCCCCGCCCCCCCCGACGATTTTCCCGGGCGACCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGATc                                       <  2:443937/151‑1 (MQ=255)                                                                                                                   ggTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGATCTTTg                                   >  1:628786/1‑151 (MQ=255)                                                                                                                     tGCGTGAAGGCGCCCACGCGCCAGCCGCCCCCACCCCCCCCACCCATTTTCCCGTGCCCTCCCGCCACATCACCGTTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGATCTTTGt                                  <  1:297255/150‑1 (MQ=255)                                                                                                                     ggCGTGAATGCGCCCCCGAGCCAGCCGCCCCCACCCCCCGCACCCAGTTTTCCGTGCCCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGATCTTTGt                                  <  1:1066099/149‑1 (MQ=255)                                                                                                                       cgggAAGGCGCCCCCGAGCAAGCCCCCCCCGGCCCCCACGCCCGTTTTTCCGTGCGCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGATCTTTGTCg                                <  1:178083/147‑1 (MQ=255)                                                                                                                             tccgcccgcGACCCAAACCCACCCCGCCCCCCCAACCCTTTTTCCGGGCCCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGATCTTTGTCGTAATGcc                         <  1:308382/143‑1 (MQ=255)                                                                                                                                      gAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGCGAGTAGCCACTCAGATCTTTGTCGTAATGCCAGATCAtgt                >  2:132420/1‑151 (MQ=255)                                                                                                                                       aGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGATCTTTGTCGTAATGCCAGATCAtgtt               >  1:837587/1‑151 (MQ=255)                                                                                                                                       aGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGATCTTTGTCGTAATGCCAGATCAtgtt               >  1:589044/1‑151 (MQ=255)                                                                                                                                            gACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGATCTTTGTCGTAATGCCAGATCATGTTGTTCa          >  1:194334/1‑151 (MQ=255)                                                                                                                                            gACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGATCTTTGTCGTAATGCCAGATCATGTTGTTCa          >  1:943768/1‑151 (MQ=255)                                                                                                                                             acccccccACCCCCCACGACCGTTTTCCCGGGCCCCCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGATCTTTGTCGTAATGCCAGATCATGTTGTTc           <  1:372597/144‑1 (MQ=255)                                                                                                                                                  cccAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGCGAGTAGCCACTCAGATCTTTGTCGTAATGCCAGATCATGTTGTTCAGGCCGa    >  2:1156812/1‑151 (MQ=255)                                                                                                                                                    cAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGATCTTTGTCGTAATGCCAGATCATGTTGTTCAGGCCGAtt  >  1:433171/1‑151 (MQ=255)                                                                                                                                                        |                                                                                                                                                   GCGCCGTGTAGCCGAAGCCCTGACTGCTGCCGCCATCGTTGGATTGCCGCGCAGCTTCTTCCTGCCAGTCGGTCGGGATCAGATTCATCAGCGGATAACGCGGCATGGTTT‑T‑GGTGCGTGAATGCGACCACGAGCCAGACGCACCCAGCTCCCACGACCAGTTTTCCGTGCGCTCCCGCCACATCACCGGTATGGCAAACGACAGGTATTCCTGCGGACTGTAGTAACCGCCCTGACCGAGTGAGTAGCCACTCAGATCTTTGTCGTAATGCCAGATCATGTTGTTCAGGCCGATT  >  NC_000913/3685879‑3686174

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑