Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,525,413	(T)6→5	coding (391/789 nt)	yjhI ←	putative DNA‑binding transcriptional regulator

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,525,408	0	T	.	100.0%	337.8 / NA	75	coding (396/789 nt)	yjhI	putative DNA‑binding transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (37/38);  total (37/38)

TTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAGTTTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCATTGGCCGCTTAGTC  >  NC_000913/4525259‑4525552                                                                                                                                                      |                                                                                                                                                     ttttGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATTCAACTCAAGtt                                                                                                                                                     <  2:140622/151‑1 (MQ=255)                  aaaCAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCtt                                                                                                                                    <  2:887711/150‑1 (MQ=255)                      aaTTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGGTAATAATGCCTTACCCAACGCGGTAATAAGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCt                                                                                                                               <  2:148616/151‑1 (MQ=255)                        ttttttATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCtcg                                                                                                                             >  1:54775/1‑151 (MQ=255)                                 ggTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACag                                                                                                                    >  1:492519/1‑151 (MQ=255)                                  gTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACaga                                                                                                                   >  2:498373/1‑151 (MQ=255)                                  gTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACag                                                                                                                    <  1:98375/150‑1 (MQ=255)                                  gTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACag                                                                                                                    <  1:283725/150‑1 (MQ=255)                                    aaaTGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGt                                                                                                                 <  2:784687/151‑1 (MQ=255)                                    aaaTGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGt                                                                                                                 <  2:704105/151‑1 (MQ=255)                                      aTGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTcc                                                                                                               <  2:492989/151‑1 (MQ=255)                                       tGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCTAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCg                                                                                                              >  1:283166/1‑151 (MQ=255)                                         tATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGa                                                                                                            <  2:408056/151‑1 (MQ=255)                                           ttGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACt                                                                                                          <  2:1026830/151‑1 (MQ=255)                                           ttGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACt                                                                                                          <  2:1007045/151‑1 (MQ=255)                                           ttGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACt                                                                                                          <  2:300547/151‑1 (MQ=255)                                             gcgcGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACttt                                                                                                        >  2:688805/1‑151 (MQ=255)                                                cgTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCg                                                                                                      <  1:303004/150‑1 (MQ=255)                                                  tATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGAt                                                                                                    >  1:923769/1‑150 (MQ=255)                                                  tATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGAt                                                                                                    >  1:360970/1‑150 (MQ=255)                                                  tATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGAt                                                                                                    >  2:857186/1‑150 (MQ=255)                                                  tATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGAt                                                                                                    >  2:988391/1‑150 (MQ=255)                                                        gAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGCGTCCGGACTTTCGATCTTGTCg                                                                                             >  1:855778/1‑151 (MQ=255)                                                        gAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCg                                                                                             >  2:977747/1‑151 (MQ=255)                                                        gAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCg                                                                                             >  1:617917/1‑151 (MQ=255)                                                        gAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCg                                                                                             >  2:91322/1‑151 (MQ=255)                                                          gTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCg‑‑‑‑ag                                                                                       >  2:875058/1‑151 (MQ=255)                                                            tAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑agat                                                                                     <  2:926935/151‑1 (MQ=255)                                                                 tGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑agatagat                                                                                 <  2:780128/150‑1 (MQ=255)                                                                 tGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑agatagat                                                                                 <  2:254410/150‑1 (MQ=255)                                                                 tGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑agatagat                                                                                 <  2:19712/150‑1 (MQ=255)                                                                    gTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCTTATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCT‑‑‑‑ATCTAGATAGc                                                                              >  2:915659/1‑150 (MQ=255)                                                                     tGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGc                                                                              <  2:634678/149‑1 (MQ=255)                                                                           tAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGc                                                                      <  1:905824/151‑1 (MQ=255)                                                                           tAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGc                                                                      <  2:808396/151‑1 (MQ=255)                                                                           tAAAAAATAATCCAGTGCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGc                                                                      <  2:616710/151‑1 (MQ=255)                                                                                aataatCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCtctc                                                                  >  1:878148/1‑150 (MQ=255)                                                                                aataatCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTcc                                                                 <  2:775289/151‑1 (MQ=255)                                                                                 ataatCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGatagatagatagccgaaatgct                                                                 >  1:205329/1‑136 (MQ=255)                                                                                  taatCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCAt                                                               <  2:340293/151‑1 (MQ=255)                                                                                         gTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCa                                                        >  2:792049/1‑151 (MQ=255)                                                                                         gTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCa                                                        >  1:113416/1‑151 (MQ=255)                                                                                           tcttctCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAg                                                      <  1:604367/151‑1 (MQ=255)                                                                                              tctcGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATg                                                   >  1:20560/1‑151 (MQ=255)                                                                                                   tgtCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACaa                                               <  1:856895/150‑1 (MQ=255)                                                                                                     tCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAAcc                                            <  2:297668/151‑1 (MQ=255)                                                                                                      cTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCa                                           >  1:244474/1‑151 (MQ=255)                                                                                                      cTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCa                                           >  2:541734/1‑151 (MQ=255)                                                                                                             gCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCAc                                     <  2:367433/150‑1 (MQ=255)                                                                                                                aataatGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTCCAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACtttt                                 >  2:975483/1‑151 (MQ=255)                                                                                                                aataatGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACtttt                                 >  2:234874/1‑151 (MQ=255)                                                                                                                aataatGCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCACCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACtttt                                 >  2:52083/1‑151 (MQ=255)                                                                                                                      gCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGa                           >  1:199378/1‑151 (MQ=255)                                                                                                                      gCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGa                           >  1:824793/1‑151 (MQ=255)                                                                                                                      gCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGa                           >  2:404731/1‑151 (MQ=255)                                                                                                                      gCCTTACCCAACGCGGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGa                           >  2:650592/1‑151 (MQ=255)                                                                                                                                   cgGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACATCTCCTGCAt               <  1:686538/150‑1 (MQ=255)                                                                                                                                   cgGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCAt               <  1:543929/150‑1 (MQ=255)                                                                                                                                   cgGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCAt               <  1:308165/150‑1 (MQ=255)                                                                                                                                   cgGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCAt               <  2:811609/150‑1 (MQ=255)                                                                                                                                   cgGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCAt               <  1:814330/150‑1 (MQ=255)                                                                                                                                   cgGTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGATCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCAt               <  1:909388/150‑1 (MQ=255)                                                                                                                                     ntAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCATTgg            >  2:391892/2‑151 (MQ=255)                                                                                                                                     gTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCATTgg            >  1:251157/1‑151 (MQ=255)                                                                                                                                     gTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCATTgg            >  1:567782/1‑151 (MQ=255)                                                                                                                                     gTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCATTgg            >  2:476215/1‑151 (MQ=255)                                                                                                                                     gTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCATTgg            >  2:980998/1‑151 (MQ=255)                                                                                                                                     gTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCATTgg            >  2:606546/1‑151 (MQ=255)                                                                                                                                     gTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCATTgg            >  1:315152/1‑151 (MQ=255)                                                                                                                                     gTAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCATTg             <  1:94736/150‑1 (MQ=255)                                                                                                                                      tAATATGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCGAGATAGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCAt               <  2:205381/151‑1 (MQ=255)                                                                                                                                       aaTATTCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGTCCCAAGATGACAAACCAGTCCCCTTTTCAGCGACAACTCCTGCATTGGcc          <  2:690310/151‑1 (MQ=255)                                                                                                                                        atatGCAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCATTGGCCg         >  1:1048609/1‑151 (MQ=255)                                                                                                                                             cAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGTAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCATTGGCCGCTTa     <  2:710410/150‑1 (MQ=255)                                                                                                                                             cAACTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGGCCACTTTTCAGCGACAACTCCTGCATTGGCCGCTTAg    <  2:267662/151‑1 (MQ=255)                                                                                                                                               aCTCAAG‑TTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCATTGGCCGCTTAGTc  <  2:405130/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                     TTTTGTAATTCTTCCAGAAACAATTTTTTATCGGTAAATGTATTGCGCGTATGTGGAGTTAACGTGAGTGCTTCTAAAAAATAATCCAGTTCTTCTCGTGTCTTCCAGGCTAATAATGCCTTACCCAACGCGGTAATATGCAACTCAAGTTTTTTGCCAATCCAGCTTTTGCTCGTCGGTACAGAGTCCGGACTTTCGATCTTGTCG‑‑‑‑AGATAGATAGCCGAAATGCTCTCCATCGCCCCAAGATGACAAACCAGTCCACTTTTCAGCGACAACTCCTGCATTGGCCGCTTAGTC  >  NC_000913/4525259‑4525552

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑