Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,462,889	(C)9→10	intergenic (+243/‑123)	fadL → / → yfdF	long‑chain fatty acid outer membrane transporter/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,462,880	1	.	C	90.6%	189.3 / 15.2	64	intergenic (+234/‑132)	fadL/yfdF	long‑chain fatty acid outer membrane transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base . (3/3);  new base C (18/40);  total (21/43)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.15e-01

CCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CT‑‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAA‑TCAATCAAAATGGAATAAAATCATGCTACCATCT  >  NC_000913/2462732‑2463023                                                                                                                                                           |                                                                                                                                                    cctcctGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CT‑‑cc                                                                                                                                                  <  1:632016/151‑1 (MQ=255)  ctcctGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CT‑‑cc                                                                                                                                                  >  1:832146/1‑150 (MQ=255)    cctGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CT‑‑ccccc                                                                                                                                               <  1:702202/151‑1 (MQ=255)     ctGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CT‑‑cccccc                                                                                                                                              <  1:468880/151‑1 (MQ=255)      tGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CT‑‑ccccccc                                                                                                                                             <  2:753653/151‑1 (MQ=255)      tGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CT‑‑ccccccc                                                                                                                                             <  2:599523/151‑1 (MQ=255)       gAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CT‑‑ccccccc                                                                                                                                             >  1:860401/1‑150 (MQ=255)        aGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CT‑‑cccccccc                                                                                                                                            <  1:712806/150‑1 (MQ=255)                aaaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTAt                                                                                                                                  <  2:96514/151‑1 (MQ=255)                 aaCAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CT‑‑ccccccccccctggctat                                                                                                                                  <  2:897698/151‑10 (MQ=255)                      ccTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATtgtg                                                                                                                              <  2:1037501/149‑1 (MQ=255)                         gTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCt                                                                                                                          >  2:756934/1‑150 (MQ=255)                              ccgCTTTCCCTTTCAAATCAATAAGGGGGTTCCCCAAACAAACCCTAAGGGAAAGGCATATTTTCCAGCCTTTCTCCCCG‑CCCTCCCAAAAAGTTTTCCTTTTCT‑CCTTTCCAGGA‑AACCCC‑‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATa                                                                                                                      <  2:480275/148‑1 (MQ=255)                              cAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTCCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATac                                                                                                                     <  1:850641/150‑1 (MQ=255)                                 cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTCCCCCCCCCCC‑‑TGGCTATTGTGCGCTCATacac                                                                                                                   <  2:129076/150‑1 (MQ=255)                                 cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATacac                                                                                                                   <  2:894079/149‑1 (MQ=255)                                 cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATacac                                                                                                                   <  2:559402/149‑1 (MQ=255)                                 cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATacac                                                                                                                   <  2:809479/149‑1 (MQ=255)                                 cTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑ATC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATacac                                                                                                                   <  1:67561/149‑1 (MQ=255)                                        tttCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTaa                                                                                                          >  1:722554/1‑151 (MQ=255)                                            aaaTCCATAAGCGGTATCCAAAGGCAAACCCGAAGGGGAAGGCATTTTTT‑CAGGCGTTCTGCTCCGCCCTCTCAAACAG‑TTTTCTTTTCTTCATTTCCAGGA‑GA‑‑CTCCCCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGa                                                                                                       <  2:249814/151‑1 (MQ=255)                                            aaaTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                                      <  1:503903/151‑1 (MQ=255)                                            aaaTCAATAAGCGGTATCCAAAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCCTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGAt                                                                                                      <  1:735638/151‑1 (MQ=255)                                             aaTCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CT‑‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAg                                                                                                    >  2:693193/1‑151 (MQ=255)                                               acAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                                                                   <  1:112460/150‑1 (MQ=255)                                                cAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGt                                                                                                  <  2:647477/151‑1 (MQ=255)                                                      gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaa                                                                                            >  1:662901/1‑151 (MQ=255)                                                       cGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGTTTCtaaat                                                                                           <  1:848143/151‑1 (MQ=255)                                                       cGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaat                                                                                           <  2:848354/151‑1 (MQ=255)                                                       cGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaat                                                                                           <  2:262348/151‑1 (MQ=255)                                                       cGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaat                                                                                           <  1:952624/151‑1 (MQ=255)                                                       cGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaat                                                                                           >  1:271609/1‑151 (MQ=255)                                                       cGGTATCCACAGACAAACCGGAAGGGAAAGGCATATTTT‑CATGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTTCTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATATGTTCtaaat                                                                                           <  2:640849/151‑1 (MQ=255)                                                        ggTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaata                                                                                          >  1:739618/1‑151 (MQ=255)                                                            tCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATg                                                                                      <  2:850864/151‑1 (MQ=255)                                                            tCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATg                                                                                      <  2:569280/151‑1 (MQ=255)                                                                    cAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtttt                                                                              >  2:388821/1‑151 (MQ=255)                                                                      aaCCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtttttt                                                                            >  1:628591/1‑151 (MQ=255)                                                                       aCCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTg                                                                           <  1:148319/151‑1 (MQ=255)                                                                       aCCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTg                                                                           >  2:56531/1‑151 (MQ=255)                                                                         cTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGAt                                                                         <  2:912041/151‑1 (MQ=255)                                                                            aaGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                                                                       <  1:853864/150‑1 (MQ=255)                                                                                 aaaGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAtt                                                                 <  2:433852/151‑1 (MQ=255)                                                                                    ggCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACTCTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAttt                                                                >  2:39663/1‑149 (MQ=255)                                                                                    ggCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa                                                              >  1:209810/1‑151 (MQ=255)                                                                                     gCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCTAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCCCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTc                                                               <  2:165660/149‑1 (MQ=255)                                                                                     gCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTc                                                               <  1:756672/149‑1 (MQ=255)                                                                                     gCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTc                                                               <  1:760394/149‑1 (MQ=255)                                                                                      cATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTCCTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAtt                                                            <  2:913123/151‑1 (MQ=255)                                                                                      cATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                                             <  1:706463/150‑1 (MQ=255)                                                                                      cATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt                                                             <  2:1022464/150‑1 (MQ=255)                                                                                          tttt‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGt                                                        <  2:944601/151‑1 (MQ=255)                                                                                                  gCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatata                                                  <  1:283414/150‑1 (MQ=255)                                                                                                  gCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatat                                                   <  2:266314/149‑1 (MQ=255)                                                                                                   cGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatatata                                                <  2:457478/151‑1 (MQ=255)                                                                                                   cGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatatat                                                 <  2:816653/150‑1 (MQ=255)                                                                                                   cGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTACATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatatata                                                <  2:747630/151‑1 (MQ=255)                                                                                                     ttCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATAtt                                              >  1:248924/1‑151 (MQ=255)                                                                                                     ttCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATAtt                                              >  2:489239/1‑151 (MQ=255)                                                                                                     ttCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CT‑‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATAtt                                              <  2:832426/150‑1 (MQ=255)                                                                                                       ctccTCGTCCTTCTCAAAGGG‑TTTACTTTTCTGCATTTCCAGGATTA‑‑TC‑‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATTCACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTg                                            <  2:920228/148‑1 (MQ=255)                                                                                                       cTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTg                                            <  2:641335/151‑1 (MQ=255)                                                                                                       cTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTg                                            >  1:630931/1‑151 (MQ=255)                                                                                                       cTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTg                                            <  2:727709/151‑1 (MQ=255)                                                                                                         gCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTa                                          >  1:449314/1‑151 (MQ=255)                                                                                                                         gag‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGAAATATATATTTGTAACAAA‑TCAATCAAAAt                         >  2:1017372/1‑151 (MQ=255)                                                                                                                          ag‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TTGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTTTAACAAA‑TCAATCAAAATg                        >  2:972840/1‑151 (MQ=255)                                                                                                                           g‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CT‑‑CCCCCCCCC‑‑CGGCCTTTTTGCCGTCATTCACTCCAATTTAAAATAAGTTTCTAATTAATGAGCGGTTTTTTATAATCCATTTTCTTTAGTAATTTATAATTGTATCAAATTCATTCAAATTga                       >  1:514021/1‑150 (MQ=255)                                                                                                                                         cATTTCCAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAA‑TCAATCAAAATGGAATAAAATCATGc          <  1:823921/151‑1 (MQ=255)                                                                                                                                             tCCAGGA‑TA‑‑CT‑‑CCCCCCCCCCTTGGCT‑TTGGGGGCTCATAAACCCAAATTAAAGATAGGGTCTTAAAAAATGGGGGTTTTTTTGTAGGCTATTTCTTTAGGGAATATTTATTTGTAAAAAA‑TCAATCAAAATGGAATAAAATCATTCTAc       >  2:675211/1‑150 (MQ=255)                                                                                                                                              ccAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAA‑TCAATCAAAATGGAATAAAATCATGCTACCa     >  1:941140/1‑151 (MQ=255)                                                                                                                                              ccAGGA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAA‑TCAATCAAAATGGAATAAAATCATGCTACCa     >  1:1051569/1‑151 (MQ=255)                                                                                                                                                  gA‑TA‑‑CTC‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAA‑TCAATCAAAATGGAATAAAATCATGCTACCATCt  <  1:659774/150‑1 (MQ=255)                                                                                                                                                           |                                                                                                                                                    CCTCCTGAGTTTTGCAAACAGCCTGTTGGCAGCTTGCCCTTTCAAATCAATAAGCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTT‑CAGGCGTTCTGCTCGTCCTTCTCAAAGAG‑TTTACTTTTCTGCATTTCCAGGA‑TA‑‑CT‑‑CCCCCCCCC‑‑TGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATATATTTGTAACAAA‑TCAATCAAAATGGAATAAAATCATGCTACCATCT  >  NC_000913/2462732‑2463023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑