New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 352814256 (0.840)6 (0.100) 6/278 5.9 10.2% coding (1674/2136 nt) yrfF putative RcsCDB‑response attenuator, inner membrane protein
?NC_000913 = 3528152 53 (0.850)coding (1684/2136 nt) yrfF putative RcsCDB‑response attenuator, inner membrane protein
Rejected: Coverage evenness skew score above cutoff.
Rejected: Frequency below cutoff threshold.

CTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3527987‑3528142
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcgccgtttCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTACTGTTGCCGAGA  <  NC_000913/3528152‑3528007
                                                                                                                                                                                                                                                                                                              
CTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGC                                                                                                                                                         <  2:121053/151‑1
                                                  GGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGAGAACCTGGTGGCAACCTCCACCGCGTCGTTTCATGCGGGATAAACGGCGCGGCGGAGGTTGCCACCAGGTTATCCA                                                                                                       >  1:663351/1‑151
                                                                             CGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATA                                                                            >  1:727303/1‑151
                                                                                   CGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGGAATAACACAT                                                                      >  1:717262/1‑151
                                                                                                         GTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTTCATGCGTGCTAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGGAATAACACATTCCCGCCATCGAGCTTCCCGG                                                 >  1:436789/1‑150
                                                                                                                              AACCTGGTGGCAACCTCCACCGCGCCGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCG                            >  1:344646/1‑150
                                                                                                                                                ACCGCGCCGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTACTGTT         >  1:426574/1‑151
                                                                                                                                                      CCGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCCCGCCATCGAGTTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTATTGTTGCCGAG   >  2:385672/1‑151
                                                                                                                                                      CCGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGAGTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTACTGTTGCCGAG   <  2:704041/151‑1
                                                                                                                                                       CGTTTCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTACTGTTGCCGAGA  >  1:292689/1‑151
                                                                                                                                                                                                                                                                                                              
CTGAAAAATGCGCTGGTCAATCTCGGCAACAGTAAAGACTGGGACGCGCTGGTAAAACGCGCCAACGCCGGGAAGCTCGATGGCGTGAATGTGTTATTACGCCCGGTGAGTGCGGAATCGCTGGATAACCTGGTGGCAACCTCCACCGCGCCGTTT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3527987‑3528142
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cgcgccgtttCATGCGTGATAAACGGCGCGGTGGAGGTTGCCACCAGGTTATCCAGCGATTCCGCACTCACCGGGCGTAATAACACATTCACGCCATCGAGCTTCCCGGCGTTGGCGCGTTTTACCAGCGCGTCCCAGTCTTTACTGTTGCCGAGA  <  NC_000913/3528152‑3528007

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.