Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,556,2070TC55.4% 14.0 / 75.9 56S215G (AGT→GGT) rtcARNA 3'‑terminal phosphate cyclase
Reads supporting (aligned to +/- strand):  ref base T (24/1);  new base C (0/31);  total (24/32)
Fisher's exact test for biased strand distribution p-value = 5.74e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.22e-08

GCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCA  >  NC_000913/3556059‑3556350
                                                                                                                                                           |                                                                                                                                               
gCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTc                                                                                                                                                >  1:805618/1‑150 (MQ=255)
          ccTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAgtgt                                                                                                                                     >  2:489773/1‑151 (MQ=255)
             cGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAg                                                                                                                                   >  1:739584/1‑150 (MQ=255)
                         gcTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCt                                                                                                                       >  1:429762/1‑150 (MQ=255)
                             tccccgagcgaaAAAAGAGGGTCCGTTAATATTTCCACTTGCGACTACAGGCGAGACGTTATTCCCCGCCCCTGGTCCCCGCGGGGGTTTAGAAATTTTCTGTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCa                                                                                                                  <  1:580640/143‑1 (MQ=255)
                                accgccaaaaaaaagGCTTTGGGTAATATTTTCATGTGCGATGGAAAGAGAGAGGTTTTTCCCCGCCCCCGGGCCGCGCGGCTGGTTATAAAAATTCTGTACAGGCGGGGAAAATCTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACa                                                                                                               <  2:200865/142‑1 (MQ=255)
                                           aaaaGCGTGGGGTAATTTTTTCCTTTCGAACTTCAAGCGAGCCGTTTTCCCCCGCCCCCTGTCCCCGGGGCAGTTTAAAAATATTCTTTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcac                                                                                                    <  1:1018845/151‑1 (MQ=255)
                                                 tgtggtttttttttcCACTTTCGACTTAAGGCGAGCGGTTTTCCCCCGCCCCCGGGCCGCGCGGCAGGTTAGAATTTTTCTGTCCAGGAGGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGc                                                                                              <  1:132735/140‑1 (MQ=255)
                                                      gTGATTTTTCCCCTTGCGACTTCAAGCGAGACGTTATCACCCGGCCCCTGGCCGCGCGGCAGGTTATAAATTTTCTGTTCATGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAAt                                                                                          <  1:1031346/150‑1 (MQ=255)
                                                      gTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATa                                                                                         >  2:28408/1‑151 (MQ=255)
                                                       ttatatTTCCCCTTTCGACTTCAAGCGAGACGTTATTCCCCGGCCCCTGTCCGCGCGGCAGTTTAGAAATTTTCTGTTCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAg                                                                                        <  1:696074/149‑1 (MQ=255)
                                                       tGATTTTTTCTCTTGCGACTTCAAGCGAGACGTTATTCCCCGCCCCCTGGTCGCGGGGCAGGTTATGAATTTTCTGTTCATGCAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAg                                                                                        <  1:608005/151‑1 (MQ=255)
                                                        aattttttCGCTTGCGTCTACAAGCAGGACGTTTTTCCCGGCCCCCGGGTCGGGCGGCAGTTTATGAATTTTCTGTTCAGGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGa                                                                                       <  1:47712/147‑1 (MQ=255)
                                                         atatTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGaa                                                                                      >  2:866448/1‑151 (MQ=255)
                                                          tttttccccTTTCCACTTAAGGCGAGGCGTTTTTCCCCGCCCCCTGGCCGCGGGGCAGTTTATGAATTTTCTTTTAAGGAGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAAc                                                                                     <  1:489568/143‑1 (MQ=255)
                                                          tatTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGcac                                                                                                    >  1:373475/1‑136 (MQ=255)
                                                             tttCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCCCAGCAACATGGCGCGGCACACCAGCTAATAGAACtt                                                                                   >  1:947572/1‑150 (MQ=255)
                                                                    tccGACTTAAAGGGAGTCGTTTTTCCCCGGCCCCTGGGCCCGCGGCAGTTTAGAAATTTTTTGTCCAAGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGc                                                                           <  2:186454/149‑1 (MQ=255)
                                                                     tCGACTTAAAGAGAGACGGTATTCCCCGGCCCCGGGGCGCGGGGCAGTTTATAAATATTCTGTTCATGCGGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCa                                                                          <  2:44045/151‑1 (MQ=255)
                                                                         ttttaaagagggagggTATGCCCGGGCGCCGGGGCGCGGGGCGGGTTCAGCTTCTTCTGTTCATGGGGGGAAAAACCCCCCGCCAGTGTAGCGCTTTCCCGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCa                                                                          <  2:389532/134‑1 (MQ=255)
                                                                           ttCAAGCGAGGCGGTATCCCCCGCCCCCGGGTCGCGGGGCGGTTTTAGATTATTCTGTTCAGGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGc                                                                     <  2:629559/151‑1 (MQ=255)
                                                                             aaagggagggggTTTTCCCCGGCCCCGGGCCGCGCGGCAGGTTATGAATTTTCTGTCCAGGCAGGGAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGcac                                                                   <  2:495450/141‑1 (MQ=255)
                                                                                gCGAGAGGGTTTTCCCCGCCCCCCGGCCGCGCGGCAGTTTTAGAATTTTCTGTTCATCCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACaa                                                                 <  2:514584/150‑1 (MQ=255)
                                                                                   agcgggTATTCCCCGGCCCCTGCCCGCGCGGCGGTTTATAAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt                                                              <  1:407039/146‑1 (MQ=255)
                                                                                   agccgttATTCCCCGCCCCTTGGCCGCGCGGCAGTTTATGAATATTCTGTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt                                                              <  1:1000593/144‑1 (MQ=255)
                                                                                   agagGGTATCCCCCGCCCCCGGGCCGCGCGGAGGGTTATAAATATTCTGTCCAGGAAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt                                                              <  1:994573/150‑1 (MQ=255)
                                                                                   acaCGGTATTCCCCGGCCCCTGGTCGCGCGGCGGTTTATAATTTTTCTGTTCATGCAGGGAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGt                                                              <  1:523743/148‑1 (MQ=255)
                                                                                        gTATTAGCTGGCCCCTGGTCTCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGCAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                                         >  1:352773/1‑150 (MQ=255)
                                                                                        gTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACCCCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                                         >  1:578864/1‑150 (MQ=255)
                                                                                        gTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                                         >  2:13543/1‑150 (MQ=255)
                                                                                        gTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                                         >  1:1019294/1‑150 (MQ=255)
                                                                                         tATGACCCGGCCACTTGTCCCCGGGCTAGTTATTAATTTTCTTTTCCAGGGGGGAAAACCCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCcg                                                         <  1:596814/150‑1 (MQ=255)
                                                                                          gtacaCCCGGCCCCCGGGGCCCGCGGCAGTTAAAAAAAATTCTGTCCAGGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCCCGCCAGCTAATAGAGCTTCTCCACGCATCTGCACAATGTTCCcg                                                         <  2:449216/146‑1 (MQ=255)
                                                                                              aCCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATCCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTc                                                    >  1:489931/1‑150 (MQ=255)
                                                                                                    tccccAGGTCGCGCGGCAGGTTATGAATATCCTGGTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAg                                              >  2:9382/2‑151 (MQ=255)
                                                                                                     ccccTGGCCGCGCGGCGGTTAATAAATTTTTGGTCCAGGAGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGt                                             <  2:455420/151‑1 (MQ=255)
                                                                                                                     cggtttttaaaTTTTCTGTTCAGGAAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc                              <  2:858459/142‑1 (MQ=255)
                                                                                                                      ggtttttaaaTATTCTTTTCATGAGGAAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc                              <  2:435447/142‑1 (MQ=255)
                                                                                                                      ggtttATGAATTTTCGTTCAAGGGAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc                              <  2:1034521/147‑1 (MQ=255)
                                                                                                                      gggTTATAAATATTCTTTTCATGCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc                              <  2:70765/149‑1 (MQ=255)
                                                                                                                       ggTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                            >  2:198999/1‑151 (MQ=255)
                                                                                                                        tttATGAATTTTCTTTTCATGCGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACg                             <  2:255692/148‑1 (MQ=255)
                                                                                                                        tttATAAATTTTCTTTTCGTGCAGGGAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGa                            <  2:935728/149‑1 (MQ=255)
                                                                                                                        gTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                           >  1:306774/1‑151 (MQ=255)
                                                                                                                        gTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                           >  2:874323/1‑151 (MQ=255)
                                                                                                                        gTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGAt                           >  1:613418/1‑151 (MQ=255)
                                                                                                                        gTTAAGAATTTTCTGTTCAGGGGGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAAc                              <  2:646268/148‑1 (MQ=255)
                                                                                                                         ttATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATg                          >  1:995941/1‑151 (MQ=255)
                                                                                                                           aTGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGc                         >  2:285899/1‑150 (MQ=255)
                                                                                                                            tGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCa                       >  2:810597/1‑151 (MQ=255)
                                                                                                                               atatTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACgg                    >  2:528287/1‑151 (MQ=255)
                                                                                                                                       ttccaggaaGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc             <  1:942303/143‑1 (MQ=255)
                                                                                                                                       tccATGCAGGAAAAAACCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGAc             <  1:323467/148‑1 (MQ=255)
                                                                                                                                             gCAGGAAAAACCCCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCg        <  1:809516/150‑1 (MQ=255)
                                                                                                                                                  gAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGGTGCCACGGGCGAGACTTCCGTTGcca  >  2:863432/1‑151 (MQ=255)
                                                                                                                                                  gAAAAACTCCACGCCAGTGTAGCGATTTCACGCTAAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTTCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGcca  >  2:829118/1‑151 (MQ=255)
                                                                                                                                                           |                                                                                                                                               
GCCGCAACCACCTCGGCACTGACGCGCTTTTCACCGACGACAAAAAAGCGTTCGGTGATATTTTCACTTTCGACTTCAAGCGAGACGGTATTACCCGGCCCCTGGTCGCGCGGCAGGTTATGAATATTCTGTTCATGCAGGGAAAAACTCCCCGCCAGTGTAGCGATTTCACGCTCAGCAACATGGCGCGGCACACCAGCTAATAGAACTTCTCCACGCATCTGCACAATGTTCCCGCGCTCGCCAAGTTGCAAGGTGTTAAACGATGCCACGGGCGAGACTTCCGTTGCCA  >  NC_000913/3556059‑3556350

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: