Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,665,702	0	C	A	54.4%	27.9 / 103.9	79	A37S (GCG→TCG)	gadX	acid resistance regulon transcriptional activator; autoactivator
Reads supporting (aligned to +/- strand):  ref base C (20/16);  new base A (0/43);  total (20/59)
Fisher's exact test for biased strand distribution p-value = 2.76e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.85e-07

GTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAAT‑ACAAAATTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAC  >  NC_000913/3665553‑3665840                                                                                                                                                            |                                                                                                                                               gTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAAT‑ACAAAATTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑Ca                                                                                                                                               >  1:1009744/1‑151 (MQ=255)       aaCGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAAT‑ACAAAATTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑cc                                                                                                                                        <  1:542632/151‑1 (MQ=255)                tGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAAT‑ACAAAATTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCg                                                                                                                            <  2:182516/151‑1 (MQ=255)                  aagaaACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAAT‑ACAAAATTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGc                                                                                                                           <  2:782318/150‑1 (MQ=255)                        cTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAAT‑ACAAAATTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTaaa                                                                                                                    >  1:37762/1‑151 (MQ=255)                            tttATGGGAAGGAAGATTTAATGCCCCCTCCTTGAGCATGGGAAAAAAAAATAAAAGCGTGCCCCTTAACACAAAT‑AAAAAATTTT‑CAA‑CACATTTATCTACTCAAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAg                                                                                                                <  2:562492/151‑1 (MQ=255)                                          gATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAAT‑ACAAAATTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCAtt                                                                                                  >  2:390446/1‑151 (MQ=255)                                            tttAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAAT‑ACAAAATTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTaa                                                                                                >  2:73100/1‑151 (MQ=255)                                            tttAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAAT‑ACAAAATTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTaa                                                                                                >  1:92645/1‑151 (MQ=255)                                              taacccctcctcctTGAGCATCGGAAAAAATAATGAAGGCGTTCCCCTTGACACAAAA‑AAAAAATTTT‑AAC‑CACACTTATCTACTAAAATTT‑GC‑CTTGCACCCG‑AAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                              <  1:366060/146‑1 (MQ=255)                                              taacccccccccctTGACCATGGGGAAAAAAAATGAAGGCGGTTCCCTTGACACGAAA‑AAAAAATTTA‑CAA‑CCCACTTATCTACTCGAATTT‑GG‑CTTCCACCCG‑AAAAAC‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                              <  2:85666/140‑1 (MQ=255)                                              tAATGCCTCCTCCGTGAGCATCGGGAAAAATAATAAACGCGTGTCCCTTGACACAAAA‑AAAAAATTTT‑CAA‑CACCCTTATCTTCTCAATTTT‑GG‑TTTGCACCCG‑AAAAAC‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                              <  1:425347/151‑1 (MQ=255)                                              tAATCCCCCCTCCTTGAGCAGCGGGAAAAATAAGAAAAGCGTCTCCTTTGACACGAAT‑AAAAAATTTT‑CAA‑CACACTTACCTACAAAAATTG‑GG‑CTTGCACCCA‑AAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                              <  1:416985/151‑1 (MQ=255)                                              tAATCCCCCCCCCTTGAACATCGGGAAAAATAATGAAAGCGTTCCCCTTGACCAAAAT‑ACAAAATTTA‑CAA‑CACCCTTATTTCCCAAATTTT‑GT‑TTTCCACCCA‑AAAAAC‑CCA‑GG‑T‑CCCCGCCATTAAAATAGCGATATTCACCATTAAcc                                                                                              <  1:28022/151‑1 (MQ=255)                                                  ccctcctcctGGAGCATCGGGAAAAATAATGAAAGTTTGTCCTTTGACAAAAAT‑ACAAAATTTC‑CAA‑CCCACTTTCCTCCT‑GAATTT‑TG‑CTTTCCACCA‑AAAAAA‑CCA‑GG‑TCCACCGCCATTAAAATAGCGATATTCACCATTAACCATgg                                                                                          <  1:567244/150‑1 (MQ=255)                                                    cccctcctgaagatacGGGAAAAAAAATAAAGGGGTTCCCTTTGCCACAAAT‑AAAAATTTTC‑CAC‑CCCCCTTTTCTCCTCAATTTT‑GG‑TTTCCA‑CCC‑CAAAAA‑ACA‑GGTT‑CCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTg                                                                                        <  2:615292/136‑1 (MQ=255)                                                     tcctcctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAAT‑ACAAAATTTT‑CAA‑CCCACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGCTATTCACCATTAACCATGGTga                                                                                       >  2:207218/1‑151 (MQ=255)                                                         cctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAAT‑ACAAAATTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                                                                                   >  1:60419/1‑151 (MQ=255)                                                         cctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAAT‑ACAAAATTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                                                                                   >  1:999054/1‑151 (MQ=255)                                                         cctTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAAT‑ACAAAATTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAat                                                                                   >  1:15385/1‑151 (MQ=255)                                                            tGAGCATCGGGAAAAAAAATAAAAGCGTGTCCCTTGACACAAAT‑AAAAAATTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCACCCG‑AAAAAA‑CAA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAatata                                                                                <  2:530027/151‑1 (MQ=255)                                                                tcatgggAAAAATTATTAAAGGCTTCCCCTTGACCAGAATGCCAAAATTTC‑CAA‑CACATTTTTCT‑GTTGAATTT‑GG‑CTTCACCCCGCCAAAAA‑CGT‑GG‑T‑CTCCGCCATTAAAATAGCGATATGCTCCATTAGCCATGGTGAGAATATAtt                                                                              <  1:144336/146‑1 (MQ=255)                                                                cagggggAAAAATAATGAAAGCGTTTCCCTTGACACAAAA‑AAAAAATTTT‑CAA‑CCCCCTTATTTACTAGATTTT‑GG‑TTTGCACCCG‑AAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                              <  2:223265/145‑1 (MQ=255)                                                                cagcGGGAAAAATAATAAAGGCGTGTCCCTTGCAAAGAAT‑AAAAAATTTA‑CAA‑CACCCTTATCTACTCGAATTT‑GG‑CTTGCACCCG‑AAAAAA‑CCG‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                              <  1:622815/146‑1 (MQ=255)                                                                cATCGGGAAAAATAATGAAAACGTGGCCCTGGACAAAAAA‑AAAAAATTAA‑CAA‑CCCTATTAAAAACTCGAATTT‑GG‑CTTACATCCA‑AAAAAC‑CCA‑GT‑T‑TTCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAttt                                                                             <  2:926202/150‑1 (MQ=255)                                                                cATCGGGAAAAAAAATGAAAGCGTCCCCCTTGACACGAAA‑AAAAAATTTT‑CAA‑CACACTTATCTCCTCAAATTT‑GG‑CTTGCACCCA‑AAAAAC‑CCG‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATAtt                                                                              <  2:71648/149‑1 (MQ=255)                                                                   cGGGAAAAAAAATAAAGGCTTGTCCCTTGACACAAAT‑ACAAAATTTC‑CAA‑CCCATTTATCTCCTCGATTTT‑TG‑CTTGCATCCC‑AAAAAA‑CCA‑GT‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                         <  2:521527/151‑1 (MQ=255)                                                                   cGGGAAAAAAAAAAAAAGCGTTTCCCTTGACACAAAA‑AAAAATTTTT‑CAA‑CCCCCTTATCTACCAAATTTT‑GG‑TTTCCACCCG‑AAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                         <  2:947097/151‑1 (MQ=255)                                                                    gggAAAAATAATGAAAGCGTGTCCCTTAACACGAAA‑AAAAAATTTA‑CAA‑CACATTTATCAACTGGATTTT‑GG‑TTTTCACCCG‑AAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                         <  2:1032754/150‑1 (MQ=255)                                                                    gggAAAAATAATAAAATCGTTCCCTTTGACACAAAT‑AAAAATTTTT‑CAC‑CACACTTTTCTCCTCGAATTT‑GT‑TTTGCATCCA‑CAAAAA‑CCA‑GG‑T‑CTCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                         <  2:118205/150‑1 (MQ=255)                                                                    gggAAAAAAAATAAAGGCGTGCCCTTTGCCAAAAAT‑AAAAATTTTT‑AAA‑CACATTTTTCCACGAAAATTG‑GG‑CTTCCATCCG‑AAAAAA‑CCG‑GG‑C‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                         <  2:1010103/150‑1 (MQ=255)                                                                    ggaaaaaaaaaTGAAAGCGTCTCCCTTAAAACAAAA‑AAAAAATTTT‑CAC‑CCCATTTATCTCCCCAAATTT‑GG‑TTTGCACCCG‑AAAAAA‑CAA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGt                                                                         <  2:1053841/145‑1 (MQ=255)                                                                      gaaaaaTAATAAAGGCGTTTCCTTTGACAAAAAT‑ACAAAATTTC‑AAA‑CACCTTTATCTACTCGATTTT‑GG‑CTTCCACCCG‑AAAAAA‑CCA‑GT‑T‑CCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCt                                                                       <  2:545355/147‑1 (MQ=255)                                                                           ataatGAAAGCGTGTCCCTTGACACGAAT‑ACAAAATTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATa                                                                 >  2:1022608/1‑151 (MQ=255)                                                                                       ttccctttGACACAAAA‑AAAAAATTTC‑CAC‑CCCTATTACCTACCCAATTTT‑GG‑CTTGCACCCC‑CAAAAA‑CCG‑GG‑T‑CTCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                      <  2:163671/144‑1 (MQ=255)                                                                                       ttccccTTGACAAAAAA‑AAAAATTTTA‑CAC‑CACACTTATCTCCTCAATTTT‑GG‑TTTGCACCCG‑AAAAAA‑CCG‑GT‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                      <  2:654755/147‑1 (MQ=255)                                                                                       tgtCCTTTGACACAAAT‑ACAAAATTTA‑CAA‑CACACTTATCTACTAGATTTT‑GC‑CTTGCATCCA‑AAAAAA‑CCG‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                      <  1:67383/150‑1 (MQ=255)                                                                                       tgtCCTTTGACAAAAAA‑AAAAAATTTC‑CAC‑CACACTTATCTACACAATTTT‑GG‑CTTGCACCCA‑AAAAAA‑CCG‑GT‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                      <  1:205086/150‑1 (MQ=255)                                                                                       tcccccTTGACACAAAA‑AAAAAATTTC‑CAC‑CACCCTTATCTACCCGATTTT‑GG‑CTTGCCCCCA‑AAAAAA‑CCG‑GG‑C‑CCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGAc                                                      <  2:37768/147‑1 (MQ=255)                                                                                        gtCCCTTGACACGAAT‑ACAAAATTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACaa                                                    >  1:907726/1‑151 (MQ=255)                                                                                          cccTTAACAGAAAT‑CAAAAATTTA‑CAA‑CCCATTTAGTTACTCAAATTT‑GG‑CTTTAACCCA‑AAAAAA‑CCA‑GG‑T‑ATCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATA‑TTATGTCTTGCATACGCAATGAGACAATTc                                                 <  2:486796/151‑1 (MQ=255)                                                                                            cTTGACACAACA‑AAAAATTTTT‑CAA‑CTCTTTTCTCTACTAGAATTT‑GG‑TTTCCACCCG‑AAAAAC‑CGA‑GG‑T‑CCCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTcc                                                <  2:966686/151‑1 (MQ=255)                                                                                                 cacGAAT‑AAAAAATTTC‑CAA‑CACACTTATCTACTCAAATTT‑GG‑CTTGCACCCA‑AAAAAC‑CAA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                           <  1:858047/151‑1 (MQ=255)                                                                                                  cacaaaaaaaaaaTTTT‑CAC‑CACATTTATCTACTCAAATTG‑GG‑TTTGCACCCA‑AAAAAC‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                           <  2:311430/142‑1 (MQ=255)                                                                                                   cacaaacaaaaaTTTTCCAA‑CACTTTTATCTACTCGAATTG‑GG‑CTTCCCTCCG‑AAAAAC‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCCCCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGt                                           <  2:11779/143‑1 (MQ=255)                                                                                                   caaaT‑AAAAATTTTT‑CAA‑CACACTTATCTACTCAATTTT‑GC‑CTTGCATCCA‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                         <  1:81292/149‑1 (MQ=255)                                                                                                   caaaT‑AAAAATTTTT‑AAA‑CCCATTTATCTACTCAAATTT‑GG‑CTTGCA‑CCC‑AAAAAA‑CCAGGG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                         <  2:842965/149‑1 (MQ=255)                                                                                                   caaaT‑AAAAAATTTT‑CAA‑CACCCTTATCTACTCAATTTT‑GG‑TTTCCATCCG‑CAAAAA‑CCA‑GT‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAg                                         <  1:171051/149‑1 (MQ=255)                                                                                                    aaaT‑AAAAAATTAA‑CAA‑CACATTTACCATCTGAATTTT‑CT‑GTTGCATCCG‑AAAAAA‑CCG‑TA‑A‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATg                                            <  1:962528/146‑1 (MQ=255)                                                                                                              aTTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑c                                                                                                                                >  2:105587/1‑54 (MQ=255)                                                                                                              aTTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑c                                                                                                                                <  1:105575/54‑1 (MQ=255)                                                                                                                 ttccAA‑CCCACTTTTCTACTCAAATTT‑GT‑TTTGCACCCA‑AAAAAA‑CCG‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                             <  1:390311/148‑1 (MQ=255)                                                                                                                 tt‑CAACCACACTTATCTCCCCAATTTT‑GT‑CTTCCACCCA‑AAAAAA‑CCA‑GG‑C‑AACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGt                             <  1:291696/151‑1 (MQ=255)                                                                                                                  t‑CAA‑CACATTTATCTACTCAATTTT‑GG‑TTTGCCCCCA‑AAAAAA‑CCG‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTg                           <  2:764993/151‑1 (MQ=255)                                                                                                                    tcaccccacTTATCTACTCAATTTT‑GG‑TTTGCAGCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTg                           <  1:919477/145‑1 (MQ=255)                                                                                                                      a‑cacacTTATCTACTCAATTTT‑GG‑CTTCCCTCCG‑CAAAAA‑CCG‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACt                        <  2:148623/151‑1 (MQ=255)                                                                                                                         caccTTATCTACCCGATTTG‑GG‑TTTGCACCCG‑AAAAAC‑CCA‑GT‑C‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                      <  2:736624/148‑1 (MQ=255)                                                                                                                         acacTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                      >  2:862166/1‑151 (MQ=255)                                                                                                                         acacTTATCTACCCAATTTT‑GG‑CTTCCACCCG‑AAAAAA‑CCG‑GG‑T‑CGCCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                      <  2:336960/151‑1 (MQ=255)                                                                                                                         acacTTATCTAC‑CCAATTT‑GGTTTTGCACCCA‑AAAAAC‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                      <  2:999410/151‑1 (MQ=255)                                                                                                                          ccacttacctcaccaaTTTGGG‑CTGGCACCCG‑CAAAAA‑CAA‑GT‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTa                      <  1:954763/137‑1 (MQ=255)                                                                                                                          cacTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                     >  2:601034/1‑151 (MQ=255)                                                                                                                          cacTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                     >  1:96593/1‑151 (MQ=255)                                                                                                                          cacTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTaa                     >  1:451454/1‑151 (MQ=255)                                                                                                                            cTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                   >  2:65460/1‑151 (MQ=255)                                                                                                                            cTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                   >  2:1042342/1‑151 (MQ=255)                                                                                                                            cTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAata                   >  2:1002689/1‑151 (MQ=255)                                                                                                                             ttttctcCTCAATTTT‑GG‑CTTGCACCCA‑AAAAAC‑CCG‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                  <  2:246652/148‑1 (MQ=255)                                                                                                                             ttATCTATTAAATTTT‑GG‑TTTTCACCCA‑AAAAAA‑CAG‑GT‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                  <  2:493429/151‑1 (MQ=255)                                                                                                                             ttATCTACTAAATTTT‑GG‑CTTACACCCA‑AAAAAA‑CCG‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAatat                  <  1:357783/151‑1 (MQ=255)                                                                                                                                  tACTCAATTTT‑GG‑CTTGCATCCG‑AAAAAC‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCAACATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt             <  2:852542/151‑1 (MQ=255)                                                                                                                                  tACTCAATTTG‑GG‑TTTCCATCCA‑CAAAAC‑CCA‑GT‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt             <  1:56256/151‑1 (MQ=255)                                                                                                                                  aacgcaaaTTT‑GT‑CTTGCACCCG‑AAAAAA‑CCG‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACAt             <  1:239906/145‑1 (MQ=255)                                                                                                                                      caaaTTT‑GC‑CTTGCACCCG‑AAAAAC‑CGA‑GG‑T‑AACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAAc         <  1:853820/149‑1 (MQ=255)                                                                                                                                        atttt‑GG‑TTTGCATCCA‑AAAAAA‑CCA‑GT‑C‑AACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat       <  2:278044/149‑1 (MQ=255)                                                                                                                                        aaTTT‑GT‑CTTGCTCCCA‑AAAAAC‑CCA‑GT‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat       <  1:226426/151‑1 (MQ=255)                                                                                                                                        aaTTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACat       >  2:439029/1‑151 (MQ=255)                                                                                                                                               g‑CTTGC‑TCCC‑CAAAAACCCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc  <  2:15388/150‑1 (MQ=255)                                                                                                                                                atttGCATCCG‑AAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAc  <  1:686519/148‑1 (MQ=255)                                                                                                                                                            |                                                                                                                                               GTGATGAACGAGTAATGAAGAAACTTTTTTATGTGCATGAAGATTTAATGCCTCCTCCTTGAGCATCGGGAGAAATAATGAAAGCGTGTCCCTTGACACGAAT‑ACAAAATTTT‑CAA‑CACACTTATCTACTCGAATTT‑GG‑CTTGCATCCG‑CAAAAA‑CCA‑GG‑T‑CACCGCCATTAAAATAGCGATATTCACCATTAACCATGGTGAGAATATATTTATGTCTTGCATACGCAATTAGACAATTCCCATGTAGTGATTGCATAGTTGACTTAATATTACATAAACATATTAC  >  NC_000913/3665553‑3665840

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑