Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 149,214 C→T A130T (GCC→ACC)  panB ← 3‑methyl‑2‑oxobutanoate hydroxymethyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913149,2140CT98.7% 292.9 / NA 79A130T (GCC→ACC) panB3‑methyl‑2‑oxobutanoate hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (50/28);  minor base A (1/0);  total (51/28)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CCAGCAGCTGTGCCCCAGCAGCTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAG  >  NC_000913/149067‑149361
                                                                                                                                                   |                                                                                                                                                   
ccagcaGCTGTGCCCCAGCAGCTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACg                                                                                                                                                  >  1:84435/1‑151 (MQ=255)
    cagcTTTGCCCCAGCAGCTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGtt                                                                                                                                                <  2:510395/149‑1 (MQ=255)
    cagcTGTGCCCCAGCAGCTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGtt                                                                                                                                                <  1:522549/149‑1 (MQ=255)
       cTGTGCCCCAGCAGCTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCg                                                                                                                                              <  1:285629/148‑1 (MQ=255)
         gtgCCCCAGCAGCTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAg                                                                                                                                         >  2:316213/1‑151 (MQ=255)
         gtgCCCCAGCAGCTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAg                                                                                                                                         >  1:33755/1‑151 (MQ=255)
           gCCCCAGCAGCTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCa                                                                                                                                       >  2:332240/1‑151 (MQ=255)
                  cagcTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTa                                                                                                                                 <  2:376580/150‑1 (MQ=255)
                     cTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTAc                                                                                                                                <  2:234928/148‑1 (MQ=255)
                     cTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTAc                                                                                                                                <  2:420166/148‑1 (MQ=255)
                     cTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTAc                                                                                                                                <  1:828765/148‑1 (MQ=255)
                     cTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTAc                                                                                                                                <  1:774995/148‑1 (MQ=255)
                     cTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGt                                                                                                                             >  2:752919/1‑151 (MQ=255)
                     cTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGt                                                                                                                             >  1:187207/1‑151 (MQ=255)
                     cTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGt                                                                                                                             >  1:299165/1‑151 (MQ=255)
                      ttCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCACCTGAACTTTGTACCCACCGAAAATATTCACTGACTGTGGTGTTAAAACTAAGTGAAAACATACAGGCACGGAAAATTCGGTCCGCATTTGTACGGtt                                                                                                                            >  2:791943/1‑151 (MQ=255)
                       tCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGttt                                                                                                                           <  2:966060/151‑1 (MQ=255)
                             gCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTAcc                                                                                                                      <  2:482136/150‑1 (MQ=255)
                             gCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTAcc                                                                                                                      <  1:591765/150‑1 (MQ=255)
                                 aTGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCa                                                                                                                 >  2:1024514/1‑151 (MQ=255)
                                 aTGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGGGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCa                                                                                                                 >  2:523177/1‑151 (MQ=255)
                                      tCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCa                                                                                                             >  1:91941/1‑150 (MQ=255)
                                      tCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCa                                                                                                             >  1:610283/1‑150 (MQ=255)
                                          tGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGcc                                                                                                        <  2:630115/151‑1 (MQ=255)
                                                gTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAAt                                                                                                  >  2:71121/1‑151 (MQ=255)
                                                gTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAAt                                                                                                  >  1:1017717/1‑151 (MQ=255)
                                                gTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGGCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAAt                                                                                                  >  2:365509/1‑151 (MQ=255)
                                                 ttGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAAt                                                                                                  <  2:549377/150‑1 (MQ=255)
                                                 ttGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAAt                                                                                                  <  2:25919/150‑1 (MQ=255)
                                                                 tCGCCGCGCCCCTGAACTTTGTGGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCAc                                                                                 <  1:1051410/151‑1 (MQ=255)
                                                                 tCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCCCATACAGGAACGGTCCGTTCGGTCAGCATTTGTCCGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCAc                                                                                 <  2:667306/151‑1 (MQ=255)
                                                                 tCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCAc                                                                                 <  2:869482/151‑1 (MQ=255)
                                                                 tCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCAc                                                                                 <  2:374080/151‑1 (MQ=255)
                                                                         ccccTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACg                                                                          >  2:583737/1‑150 (MQ=255)
                                                                         ccccTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACg                                                                          >  1:64546/1‑150 (MQ=255)
                                                                               aaCTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAAc                                                                    <  1:1002038/150‑1 (MQ=255)
                                                                                          cACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGtt                                                        >  1:302155/1‑151 (MQ=255)
                                                                                            ccGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTTTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGtttt                                                      >  2:840433/1‑151 (MQ=255)
                                                                                               aaaaTATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGa                                                   >  2:821623/1‑151 (MQ=255)
                                                                                                  atatTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGACCGGTACGTTCGGTCAGCATTTGTACGGTTTCTCCCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAgg                                                <  1:596222/151‑1 (MQ=255)
                                                                                                  atatTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAgg                                                >  2:953121/1‑151 (MQ=255)
                                                                                                  atatTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAgg                                                >  1:166730/1‑151 (MQ=255)
                                                                                                  atatTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAgg                                                >  2:450881/1‑151 (MQ=255)
                                                                                                   tatTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGc                                               <  2:930436/151‑1 (MQ=255)
                                                                                                     ttCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCtt                                             >  2:8605/1‑151 (MQ=255)
                                                                                                        actgactgTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGtt                                          >  2:15420/1‑151 (MQ=255)
                                                                                                           gactgTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCg                                       >  1:988056/1‑151 (MQ=255)
                                                                                                            actgTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCAc                                                                                 >  1:905513/1‑108 (MQ=255)
                                                                                                            acggtggtgTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAacac                                                                                 <  2:905827/105‑1 (MQ=255)
                                                                                                             ctgTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCgg                                      >  1:133922/1‑150 (MQ=255)
                                                                                                             ctgTGGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCgg                                      >  1:304087/1‑150 (MQ=255)
                                                                                                              tgtgGTGTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCg                                    <  2:336684/151‑1 (MQ=255)
                                                                                                               gtggtgTTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGt                                   >  2:386245/1‑151 (MQ=255)
                                                                                                                  gtgtTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTgg                                 >  1:1054216/1‑150 (MQ=255)
                                                                                                                  gtgtTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTgg                                 >  2:767080/1‑150 (MQ=255)
                                                                                                                  gtgtTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTgg                                 >  2:982830/1‑150 (MQ=255)
                                                                                                                  gtgtTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTgg                                 >  2:577493/1‑150 (MQ=255)
                                                                                                                  gtgtTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTgg                                 >  2:218419/1‑150 (MQ=255)
                                                                                                                  gtgtTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTgg                                 >  1:929437/1‑150 (MQ=255)
                                                                                                                    gtTAAACCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCAt                              >  1:937182/1‑151 (MQ=255)
                                                                                                                       aaaCCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACg                           >  2:993827/1‑151 (MQ=255)
                                                                                                                       aaaCCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACg                           >  2:760533/1‑151 (MQ=255)
                                                                                                                        aaCCTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGc                          <  1:752658/151‑1 (MQ=255)
                                                                                                                          ccTAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCa                        >  2:678135/1‑151 (MQ=255)
                                                                                                                            tAAGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATa                      >  1:871567/1‑151 (MQ=255)
                                                                                                                              aGTGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATaaa                    >  2:917560/1‑151 (MQ=255)
                                                                                                                                tGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAAc                   <  1:840150/150‑1 (MQ=255)
                                                                                                                                tGACCACATACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAAc                   <  1:280313/150‑1 (MQ=255)
                                                                                                                                     acaTACAGGAACGGTACGTTCGGTCAGCATTTGGACGGTTTCTACCAGCCACTTACCGCCTTCATTTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGCGGCATACTCCATAAACGGCTgg             >  2:344010/1‑151 (MQ=255)
                                                                                                                                      caTACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGt            >  2:1056865/1‑151 (MQ=255)
                                                                                                                                       aTACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGt            >  2:743428/1‑150 (MQ=255)
                                                                                                                                       aTACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTc           <  2:219189/151‑1 (MQ=255)
                                                                                                                                        tACAGGAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTca          >  2:579617/1‑151 (MQ=255)
                                                                                                                                        tACAGGAACGGTACGTTCGGTCAGCATTTGTACGGGTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTca          >  2:492349/1‑151 (MQ=255)
                                                                                                                                            ggAACGGTACGTTCGGTCAGCATTTGTACGGTTTTTACCAGCCACTCACCGCCTTCAATTCTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTcagcca      >  2:611778/1‑151 (MQ=255)
                                                                                                                                            ggAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTcagcca      >  2:390176/1‑151 (MQ=255)
                                                                                                                                             gAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTcagccag     >  1:702487/1‑151 (MQ=255)
                                                                                                                                             gAACGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTcagcca      <  1:901830/150‑1 (MQ=255)
                                                                                                                                                cGGTACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCcagcag  >  1:793574/1‑151 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
CCAGCAGCTGTGCCCCAGCAGCTTCTAAGGCTAATGCATCGCTGAGCAGTTGATCGCCCGCTTCATCGCCGCGCCCCTGAACTTTGTAGCCACCGAAAATATTCACTGACTGTGGTGTTAAACCTAAGTGACCACATACAGGAACGGCACGTTCGGTCAGCATTTGTACGGTTTCTACCAGCCACTCACCGCCTTCAATTTTGACCATGTTAGCACCGGCACGCATAACCGTTGCGGCGTTTTCGAAGGCTTGTTCCGGCGTGGCATACGCCATAAACGGCAGGTCAGCCAGCAG  >  NC_000913/149067‑149361

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑