Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,063,365 A→G R137R (CGT→CGC)  cbpA ← DnaK co‑chaperone; curved DNA‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,063,3650AG98.0% 160.1 / NA 49R137R (CGT→CGC) cbpADnaK co‑chaperone; curved DNA‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (33/15);  minor base C (0/1);  total (33/16)
Fisher's exact test for biased strand distribution p-value = 3.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGCATGCTGACCGAAAATTGACGAGAAGATATCGTCAAAATCTTCGGC  >  NC_000913/1063221‑1063505
                                                                                                                                                 |                                                                                                                                            
gTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTa                                                                                                                                        >  1:147230/1‑151 (MQ=255)
gTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTa                                                                                                                                        >  2:390506/1‑151 (MQ=255)
   ttCGCCCGGCGTCCCCTGGCCTTTCGGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGc                                                                                                                                     <  1:593797/151‑1 (MQ=255)
   ttCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGc                                                                                                                                     <  2:142338/151‑1 (MQ=255)
   ttCCCCCGGCGCCCCCTGGCCTTTCAGACGGATGCTTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTTCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGc                                                                                                                                     <  1:317487/151‑1 (MQ=255)
           gCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAg                                                                                                                             >  1:598596/1‑151 (MQ=255)
              tCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCg                                                                                                                           <  2:361608/150‑1 (MQ=255)
                       cTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAg                                                                                                                 >  2:793640/1‑151 (MQ=255)
                        tttCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAgg                                                                                                                >  1:783623/1‑151 (MQ=255)
                         ttCAGACGGATGCGTTGACCATTGCCGCCCCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGa                                                                                                               <  2:802350/151‑1 (MQ=255)
                               cGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCg                                                                                                                           <  1:156052/133‑1 (MQ=255)
                               cGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCg                                                                                                                           >  2:156088/1‑133 (MQ=255)
                               cGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCg                                                                                                         >  2:920899/1‑151 (MQ=255)
                                 gATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGc                                                                                                        >  2:652410/1‑150 (MQ=255)
                                    gCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCa                                                                                                      >  2:457615/1‑149 (MQ=255)
                                      gTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACtt                                                                                                   >  1:719846/1‑150 (MQ=255)
                                         gACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGAt                                                                                               >  2:546741/1‑151 (MQ=255)
                                         gACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGAt                                                                                               >  1:1024973/1‑151 (MQ=255)
                                                        ccgcggGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTAAAAACCGGCAGGTTATAGCTGATGGTGCTTTTATGCTCAGTAAGGGTTTCTTCGGGAAATACCGCCACTTCGATTTCAATATCGTGGcc                                                                                <  1:190031/151‑1 (MQ=255)
                                                           ccgGGATCTTCCCTTTCGGCTTTTTCGGAATTTCCTTTT‑CGATCATGCCAAAGGCTTTATAAACCGGCAGTTTATAGCTGAGGTTGCGTTTATGCTCAGTAGGGGTTTCTTCGAGAAATACCGCCACTTCGATTTCAATATCGTGGCcgcg                                                                             <  1:237949/151‑1 (MQ=255)
                                                               gATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGtgtg                                                                         >  1:906200/1‑151 (MQ=255)
                                                                 tCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTgg                                                                        >  1:767515/1‑150 (MQ=255)
                                                                 tCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTgg                                                                        >  1:429544/1‑150 (MQ=255)
                                                                       caTTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCg                                                                 >  1:966997/1‑151 (MQ=255)
                                                                                ttttCGGAATTTCCTTTT‑CGATCATGCAAAAGGCGTTATAACCCGGCAGTTTATAGCTGATGGTGCGTTTATGCTAATTAGGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGc                                                         <  2:922348/150‑1 (MQ=255)
                                                                                  tttggAATTTCCCGTTGCGTCCATGCAAAGGGGGTTAAAACCCGGCAGGTTAAAGTTGGGGGTGCGCTTATGGTCGGAAAGCGTTTTTTCGAGGAAGACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGcta                                                       <  2:400721/148‑2 (MQ=255)
                                                                                       attttCCTTTT‑CGTTCATGCCAAGGGCGTTATAAACGGGCAGGTTATAGCTGATGTTGCCCTTTTGCTCAGTCAGCGTTTCTTCGGGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCg                                                  <  2:437390/148‑1 (MQ=255)
                                                                                          ttCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGCa                                              >  1:504794/1‑151 (MQ=255)
                                                                                                     aTCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGCATGCTGACCGa                                    >  1:171780/1‑151 (MQ=255)
                                                                                                       cATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGCATGCTGGCCGcaa                                  >  1:852846/1‑151 (MQ=255)
                                                                                                         tGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGGATGCTGACCGAAAAt                                >  1:871321/1‑151 (MQ=255)
                                                                                                         tGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGCATGCTGGCCGGAAAt                                >  1:247199/1‑151 (MQ=255)
                                                                                                         tGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGCATGCTGACCGAAAAt                                >  1:223306/1‑151 (MQ=255)
                                                                                                         tGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCGCTGGCGGGCATGCTGGCCGAAAAt                                >  1:432771/1‑151 (MQ=255)
                                                                                                         tGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGGTGACGGCTCTGGCGGGCATGCTGACCGAAAAt                                >  1:673250/1‑151 (MQ=255)
                                                                                                           ccAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGGCTGGTGGCCGGAAAATg                              >  1:831428/1‑151 (MQ=255)
                                                                                                             aaggcctttATAACCCGGCGGGTTATAGCGGATGTTGCGTTTATGCTCAAAAGGCGTTTCTTGGAGGAATCCCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGCATGCTGACCGAAAATTGAc                            <  1:275372/144‑1 (MQ=255)
                                                                                                                   gTTATAAACCGGCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGGTGACGGCTCTGGCGGGCATGCTGACCGAAAATTTAAGggaag                      >  2:214700/1‑151 (MQ=255)
                                                                                                                     tttaACCCGGCGGGTTATGGTTGGTGTTGCGTTTTTGTTCAGTAGGGTTTTGTTGGAGGAATCCCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGCATGCTGACCGAAAATTGACGAGAAGat                    <  2:477090/149‑1 (MQ=255)
                                                                                                                       aaacccGGCAGTTTAAAGTTGTGGGTGCTTTTATGCTCAGTAGGCGTTTCTTCGGGGAATCCCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGCATGCTGACCGAAAATTGACGAGAAGatat                  <  2:753384/147‑1 (MQ=255)
                                                                                                                              gCAGGTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACAGCTCTGGCGGGATTGCTGGCCGAAAATTGACCAGGAGATATCGGCaaa           >  2:783475/1‑151 (MQ=255)
                                                                                                                                 ggTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGGCTGCTTACCGGAAATTGGCGAGAGGATATGGTCCAAAtt        >  2:463826/1‑150 (MQ=255)
                                                                                                                                 ggTTATAGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGCATGCTTCCCGAAAATTTACGAGAAGATATCGTCAAAAt         >  2:625669/1‑150 (MQ=255)
                                                                                                                                  gTTATAGCTGTTGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGCATGGTGAACGGAAATTGACGAGAAGATATCGTTAAAATc        >  2:353994/1‑150 (MQ=255)
                                                                                                                                  gTTATAGCTGATGGTCGCCTTATGCTCAGTAAGGGTTTTTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGCATGCTGACCGAAAATTGACGAGAAGATATCGTCAAAAt         <  2:879473/149‑1 (MQ=255)
                                                                                                                                    tataGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGCATGCTGACCGAAAATTGACGAGAAGATATCGTCAAAATCTTc     <  1:824954/151‑1 (MQ=255)
                                                                                                                                     ataGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGCCTGCTGACCGAAAATTGACGAGAAGATATCGTCAAAATCTTCg    >  2:612517/1‑151 (MQ=255)
                                                                                                                                       aGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGCATGCTGACCGAAAATTGACGAGAAGATATCGTCAAAATCTTCgg   >  2:581992/1‑150 (MQ=255)
                                                                                                                                       aGCTGATGGTGCGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCGCTGGCGGGCATGCTGAACGAAAAATGGCGAGGAGATATCGGCAAAATCTTCggg  >  2:684981/1‑150 (MQ=255)
                                                                                                                                                 |                                                                                                                                            
GTTTTCGCCCGGCGTCCCCTGGCCTTTCAGACGGATGCGTTGACCATTGCCGACGCCCGCCGGGATCTTCACATTCAGCGTTTTCGGAATTTCCTGTT‑CGATCATGCCAAAGGCGTTATAAACCGGCAGGTTATAGCTGATGGTACGCTTATGCTCAGTAAGCGTTTCTTCGAGGAATACCGCCACTTCGATTTCAATATCGTGGCCGCGTGTGGCGGGGCGTTGACGGCTCTGGCGGGCATGCTGACCGAAAATTGACGAGAAGATATCGTCAAAATCTTCGGC  >  NC_000913/1063221‑1063505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑