Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,359,914	G→C	A80G (GCC→GGC)	rhmT ←	putative L‑rhamnonate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,359,914	0	G	C	100.0%	166.6 / NA	49	A80G (GCC→GGC)	rhmT	putative L‑rhamnonate transporter
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (19/30);  total (19/30)

GTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAAC  >  NC_000913/2359767‑2360058                                                                                                                                                    |                                                                                                                                                 gTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGccc                                                                                                                                                <  1:308637/150‑1 (MQ=255)   gagaTAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGCATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCcccc                                                                                                                                              >  2:750253/1‑150 (MQ=255)    agaTAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGcccccc                                                                                                                                             <  1:199084/150‑1 (MQ=255)      aTAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTAGCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAg                                                                                                                                           <  2:894797/150‑1 (MQ=255)        aaaTCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGGGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCAATGCCATGGCTGCCGAAAGAAATCCCAACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGttt                                                                                                                                        >  2:534817/1‑151 (MQ=255)                    ggAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCaa                                                                                                                               <  1:890284/148‑1 (MQ=255)                    ggAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAg                                                                                                                            >  1:491788/1‑151 (MQ=255)                        aaaTCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTc                                                                                                                        <  2:361040/151‑1 (MQ=255)                         aaTCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCg                                                                                                                       >  1:370643/1‑151 (MQ=255)                                   ccGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAacac                                                                                                              >  1:158343/1‑150 (MQ=255)                                   ccGAAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAccac                                                                                                              >  2:444481/1‑150 (MQ=255)                                        gcaCGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGaa                                                                                                        >  2:1018920/1‑151 (MQ=255)                                          aCGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTCTCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAAc                                                                                                      <  1:309655/151‑1 (MQ=255)                                                   agTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATAc                                                                                              <  2:32605/150‑1 (MQ=255)                                                     tGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATAcc                                                                                             <  2:439387/149‑1 (MQ=255)                                                          aCTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGa                                                                                      <  1:1034461/151‑1 (MQ=255)                                                                  aaaTTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGcc                                                                               <  2:1039300/150‑1 (MQ=255)                                                                               gTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTgcgc                                                                                                                                    <  2:384741/84‑1 (MQ=255)                                                                               gTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTgcgc                                                                                                                                    >  1:384614/1‑84 (MQ=255)                                                                                   cGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGc                                                             <  1:350713/151‑1 (MQ=255)                                                                                     gCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCtt                                                           >  2:41115/1‑151 (MQ=255)                                                                                     gCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCtt                                                           >  1:681132/1‑151 (MQ=255)                                                                                         atgccatgGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCAtt                                                       >  1:689858/1‑151 (MQ=255)                                                                                            ccatgGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTAc                                                     <  2:421909/150‑1 (MQ=255)                                                                                            ccatgGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTAc                                                     <  2:338723/150‑1 (MQ=255)                                                                                            ccatgGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCGGCACCCAGCGCATAAGCTTCATTAc                                                     <  1:847519/150‑1 (MQ=255)                                                                                             catgGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTc                                                   <  1:178042/151‑1 (MQ=255)                                                                                             catgGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTc                                                   <  1:849057/151‑1 (MQ=255)                                                                                             catgGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTc                                                   <  2:390640/151‑1 (MQ=255)                                                                                                  cTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCATccc                                              <  2:1016570/151‑1 (MQ=255)                                                                                                  cTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAAccc                                              <  2:1012220/151‑1 (MQ=255)                                                                                                  cTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAAccc                                              <  2:126197/151‑1 (MQ=255)                                                                                                  cTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAAccc                                              <  2:266615/151‑1 (MQ=255)                                                                                                        aaagaaaTCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTAt                                         <  2:465681/150‑1 (MQ=255)                                                                                                        aaagaaaTCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTAt                                         >  2:806113/1‑150 (MQ=255)                                                                                                        aaagaaaTCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTAt                                         <  2:1030408/150‑1 (MQ=255)                                                                                                        aaagaaaTCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTAt                                         <  2:882899/150‑1 (MQ=255)                                                                                                         aagaaaTCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCCTATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCa                                       >  2:658155/1‑151 (MQ=255)                                                                                                             aaTCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCt                                   <  1:805748/151‑1 (MQ=255)                                                                                                                ccccACAGCAGTGTTGTCGTACCAATCCAGTTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAGCGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGt                                <  2:378513/151‑1 (MQ=255)                                                                                                                ccccACAGCAGTGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGt                                >  1:696407/1‑151 (MQ=255)                                                                                                                     cagcagGGTTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGt                            >  1:229684/1‑150 (MQ=255)                                                                                                                        gtgtgtTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGt                            >  2:873240/3‑147 (MQ=255)                                                                                                                          gtgtTGTCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGttt                      >  1:917404/1‑151 (MQ=255)                                                                                                                           tgttgtCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGttt                      <  2:404890/150‑1 (MQ=255)                                                                                                                           tgttgtCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGtt                       <  1:186165/149‑1 (MQ=255)                                                                                                                              tgtCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCGCAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCa                  >  1:216515/1‑151 (MQ=255)                                                                                                                                tCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCaa                 <  2:1035746/150‑1 (MQ=255)                                                                                                                                tCGTACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCaa                 <  2:919587/150‑1 (MQ=255)                                                                                                                                   tACCAATCCAGGTTCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACCAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAAc              >  1:553711/1‑150 (MQ=255)                                                                                                                                               ttCTGCCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAAc  <  1:79608/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 GTGAGATAAATCATACCAGGGAAAAATCCGGCCTCCGCAGCACGAAGCAGAGTGCGAACTATCAGAAATTTCGCTTCAGTATCGGCCCATGCCATGGCTGCCGAAAGAAATCCCCACAGCAGTGTTGTCGTACCAATCCAGGTTCTGGCCCCCAGTTTGCGCATCAAAAGATTCGCCGGAACACCCAGAAACGCATATACCACAAAGAAAATGCCTGCTCCCAGCGCATAAGCTTCATTACTCAACCCGGTATCAATCTGGTAGGTCTGTTTGGCAAAACCGATATTCGAAC  >  NC_000913/2359767‑2360058

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑