Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 429,143 C→T G499G (GGC→GGT secD → SecYEG protein translocase auxillary subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913429,1430CT98.6% 253.9 / NA 69G499G (GGC→GGTsecDSecYEG protein translocase auxillary subunit
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base T (36/32);  minor base A (1/0);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAACAGGGGCTGGAGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCG  >  NC_000913/428997‑429286
                                                                                                                                                  |                                                                                                                                               
gAACAGGGGCTGGAGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCaa                                                                                                                                             >  1:549984/1‑151 (MQ=255)
gAACAGGGGCTGGAGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCaa                                                                                                                                             >  1:624960/1‑151 (MQ=255)
gAACAGGGGCTGGAGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCaa                                                                                                                                             >  1:29204/1‑151 (MQ=255)
      gggCTGGAGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGc                                                                                                                                          <  1:543741/148‑1 (MQ=255)
      gggCTGGAGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTGGTCTGGTTGCGACCAGTTCTCTTCTTGCCAAATTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAg                                                                                                                                      >  2:87493/1‑151 (MQ=255)
            gAGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGTGTATg                                                                                                                                  >  2:516322/1‑150 (MQ=255)
               gCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGcc                                                                                                                                <  2:373870/149‑1 (MQ=255)
               gCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGcc                                                                                                                                <  2:992007/149‑1 (MQ=255)
                cttgccttgcCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAgg                                                                                                                             <  2:586937/151‑1 (MQ=255)
                 ttgccttgccGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGt                                                                                                                            <  2:275469/151‑1 (MQ=255)
                   gccttgccGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTAt                                                                                                                          >  1:169432/1‑151 (MQ=255)
                            gTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTAt                                                                                                                 >  1:988666/1‑151 (MQ=255)
                                     tttCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTaa                                                                                                         >  1:415493/1‑150 (MQ=255)
                                     tttCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTaa                                                                                                         >  1:44297/1‑150 (MQ=255)
                                     tttCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTaa                                                                                                         >  1:461853/1‑150 (MQ=255)
                                       tCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAAccc                                                                                                      <  2:29210/151‑1 (MQ=255)
                                          aTTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTg                                                                                                   >  2:628922/1‑151 (MQ=255)
                                             cTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCgg                                                                                                <  1:999229/151‑1 (MQ=255)
                                               gTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTg                                                                                              >  1:295173/1‑151 (MQ=255)
                                               gTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTg                                                                                              >  1:882585/1‑151 (MQ=255)
                                               gTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTg                                                                                              >  2:873293/1‑151 (MQ=255)
                                                      atcatcTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGGGGCGGTCg                                                                                       >  2:1005189/1‑151 (MQ=255)
                                                             tctATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGaa                                                                                <  1:1014354/151‑1 (MQ=255)
                                                               tataAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACg                                                                              >  2:453613/1‑151 (MQ=255)
                                                               tataAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACg                                                                              <  2:295259/151‑1 (MQ=255)
                                                                ataAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGt                                                                             >  2:748867/1‑151 (MQ=255)
                                                                ataAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGt                                                                             >  1:155889/1‑151 (MQ=255)
                                                                     aagTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGa                                                                        >  2:745381/1‑151 (MQ=255)
                                                                     aagTTTGGGCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTGTCCAGGTGCAACGCTGGGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGACCGTACTg                                                                         <  2:519859/150‑1 (MQ=255)
                                                                       gTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGAt                                                                       >  2:286728/1‑150 (MQ=255)
                                                                        tttGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATc                                                                      <  1:488395/150‑1 (MQ=255)
                                                                        tttGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATc                                                                      <  1:699392/150‑1 (MQ=255)
                                                                        tttGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATc                                                                      <  1:725727/150‑1 (MQ=255)
                                                                        tttGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATc                                                                      <  2:87559/150‑1 (MQ=255)
                                                                        tttGGTCTGATTGCGACCAGTGCGCTGATTGCCAACTTGATCGTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAGCGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGAt                                                                       <  1:791862/149‑1 (MQ=255)
                                                                            gTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCaacga                                                                 >  2:211447/1‑151 (MQ=255)
                                                                             tCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGGGGCGGTCGATGCGAACGTACTGATCaacga                                                                 <  2:987212/150‑1 (MQ=255)
                                                                                gaatGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGt                                                             >  1:856850/4‑151 (MQ=255)
                                                                                gATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAAccc                                                                                                      <  2:464083/110‑1 (MQ=255)
                                                                                gATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAAccc                                                                                                      >  1:463896/1‑110 (MQ=255)
                                                                                gATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGt                                                             >  1:230412/1‑151 (MQ=255)
                                                                                           gTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAagaaga                                                  >  1:270222/1‑151 (MQ=255)
                                                                                           gTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAagaaga                                                  >  2:687458/1‑151 (MQ=255)
                                                                                           gTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAagaaga                                                  >  2:297076/1‑151 (MQ=255)
                                                                                           gTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAagaaga                                                  >  2:438804/1‑151 (MQ=255)
                                                                                            tGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAaagaag                                                   <  1:238157/149‑1 (MQ=255)
                                                                                             gCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCgg                                                                                                >  2:586124/1‑103 (MQ=255)
                                                                                             gCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCgg                                                                                                <  1:585880/103‑1 (MQ=255)
                                                                                                           cTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGt                                  >  2:925051/1‑151 (MQ=255)
                                                                                                           cTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGGGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGt                                  >  2:524389/1‑151 (MQ=255)
                                                                                                             tGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTAc                                <  2:1014441/151‑1 (MQ=255)
                                                                                                             tGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTAc                                <  1:845772/151‑1 (MQ=255)
                                                                                                                  ttAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTc                           <  2:992636/151‑1 (MQ=255)
                                                                                                                  ttAATCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGATGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTc                           <  1:213019/151‑1 (MQ=255)
                                                                                                                     aTCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAAc                        <  2:296596/151‑1 (MQ=255)
                                                                                                                     aTCGTCGGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAAc                        <  1:286645/151‑1 (MQ=255)
                                                                                                                       cgtcgGCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAg                      <  2:40230/151‑1 (MQ=255)
                                                                                                                            gCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTg                                                                         <  2:151832/95‑1 (MQ=255)
                                                                                                                            gCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTg                                                                         >  1:151797/1‑95 (MQ=255)
                                                                                                                            gCATTATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAAt                 >  2:317753/1‑151 (MQ=255)
                                                                                                                               ttATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTCCTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATtga              >  1:29039/1‑151 (MQ=255)
                                                                                                                               ttATGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATtga              <  1:966927/151‑1 (MQ=255)
                                                                                                                                 aTGTCGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATtgatg            >  1:164321/1‑151 (MQ=255)
                                                                                                                                 aTGTCGCTGTTGCCAGGAGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGGGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATtgatg            >  1:689872/1‑151 (MQ=255)
                                                                                                                                     cGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAgg        <  1:183630/151‑1 (MQ=255)
                                                                                                                                     cGCTGTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAgg        <  1:1031979/151‑1 (MQ=255)
                                                                                                                                         gTTGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTAt    >  2:437897/1‑151 (MQ=255)
                                                                                                                                           tGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCg  <  2:199072/151‑1 (MQ=255)
                                                                                                                                           tGCCAGGTGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCg  <  1:5598/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                               
GAACAGGGGCTGGAGGCTTGCCTTGCCGGTCTGCTGGTTTCTATTCTGTTCATGATCATCTTCTATAAGAAGTTTGGTCTGATTGCGACCAGTGCTCTGATTGCCAACTTGATCTTAATCGTCGGCATTATGTCGCTGTTGCCAGGCGCAACGCTGAGTATGCCAGGTATCGCGGGTATCGTCTTAACCCTTGCGGTGGCGGTCGATGCGAACGTACTGATCAACGAACGTATTAAAGAAGAGTTGAGCAACGGACGTACTGTTCAACAGGCAATTGATGAAGGTTATCG  >  NC_000913/428997‑429286

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: