| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,494,883 | (A)7→8 | coding (261/564 nt) | idnK → | D‑gluconate kinase, thermosensitive |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,494,876 | 1 | . | A | 98.2% | 210.1 / ‑3.8 | 54 | coding (254/564 nt) | idnK | D‑gluconate kinase, thermosensitive |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base A (37/16); total (38/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01 | |||||||||||
TCTTCATCCAGCCAAAAATATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑AAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCGTTACTAAAAAGTC > NC_000913/4494730‑4495016 | tCTTCATCCAGCCAAAAATATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑aaaa > 1:696930/1‑151 (MQ=255) ccAGCCAAAAATATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑aaaaa‑aaacag < 2:58532/151‑5 (MQ=255) aaaTATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGa < 1:1063919/151‑1 (MQ=255) tataGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGatat > 2:788197/1‑151 (MQ=255) gATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTa > 2:767474/1‑151 (MQ=255) gATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTa > 1:479391/1‑151 (MQ=255) gATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTa > 1:38214/1‑151 (MQ=255) gATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGACGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTa > 2:834945/1‑151 (MQ=255) aaaTGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCa > 1:401153/1‑151 (MQ=255) aaaTGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCACACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCa < 1:381519/151‑1 (MQ=255) gggTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGcc > 2:596256/1‑150 (MQ=255) gggTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGcc > 2:529745/1‑150 (MQ=255) gTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGcccc > 2:969035/1‑150 (MQ=255) gTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGcccc > 1:109604/1‑150 (MQ=255) aTTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCAt > 2:345590/1‑151 (MQ=255) ttCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATg > 2:163169/1‑151 (MQ=255) ccATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCAAtttt > 1:1263860/1‑151 (MQ=255) cATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTc < 1:169205/151‑1 (MQ=255) tATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAtt > 2:315768/1‑151 (MQ=255) aTCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAttt < 2:584243/151‑1 (MQ=255) tCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTc < 1:969387/151‑1 (MQ=255) tCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTc < 1:386690/151‑1 (MQ=255) aaGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTgg < 1:616877/150‑1 (MQ=255) aTCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt < 1:800222/149‑1 (MQ=255) aTCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAg > 2:1036910/1‑151 (MQ=255) gACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATg > 1:222093/1‑151 (MQ=255) aCTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATgg < 2:436108/151‑1 (MQ=255) tCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGa > 1:1190862/1‑151 (MQ=255) tCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGa > 2:1160878/1‑151 (MQ=255) tCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGa > 1:579544/1‑151 (MQ=255) cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTAtt > 1:1225902/1‑149 (MQ=255) gCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATg > 1:358160/1‑151 (MQ=255) ttCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCa > 1:637746/1‑151 (MQ=255) ttCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCa > 1:565054/1‑151 (MQ=255) ttCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCa > 1:858571/1‑151 (MQ=255) tCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAg < 1:1185909/151‑1 (MQ=255) cATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAg > 2:914972/1‑150 (MQ=255) aTACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCg > 1:428181/1‑151 (MQ=255) tACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGt < 2:738713/151‑1 (MQ=255) tCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTAGCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGc < 2:1266618/151‑1 (MQ=255) ataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGc < 1:898265/151‑1 (MQ=255) gAATGAAACAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTAt > 2:722029/1‑151 (MQ=255) aaaCAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCgg > 1:529242/1‑151 (MQ=255) aaaCAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCgg > 2:1170039/1‑151 (MQ=255) aaaCAGGATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGGGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCgg > 2:921784/1‑151 (MQ=255) ggATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCg > 1:852719/1‑151 (MQ=255) ggATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCg > 1:724704/1‑151 (MQ=255) ggATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCg > 2:948623/1‑151 (MQ=255) ggATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCAATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGAAGCg > 1:427468/1‑151 (MQ=255) ggATTTATTGTCTGTTCATCATT‑AAAAACAACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCg > 1:628185/1‑151 (MQ=255) gATTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCGt > 1:571796/1‑151 (MQ=255) aTTTATTGTCTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCGtt > 2:236786/1‑151 (MQ=255) cTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCGTTACTAAAAAg > 1:498728/1‑151 (MQ=255) cTGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCGTTACTAAAAAg < 1:1170511/151‑1 (MQ=255) tGTTCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCGTTACTAAAAAGt < 2:1241308/151‑1 (MQ=255) ttCATCATTAAAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCGTTACTAAAAAGTc < 2:27985/150‑1 (MQ=255) | TCTTCATCCAGCCAAAAATATAGATAAAATGTCGCAGGGTATTCCATTATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATT‑AAAAA‑AACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGCGTCGGGCTGGGCATTTTATGCCGGTAGCGTTACTAAAAAGTC > NC_000913/4494730‑4495016 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |